Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,913,273 T→C 77.3% N515S (AAC→AGC)  prc ← carboxy‑terminal protease for penicillin‑binding protein 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,913,2730TC77.3% 283.6 / 73.2 146N515S (AAC→AGC) prccarboxy‑terminal protease for penicillin‑binding protein 3
Reads supporting (aligned to +/- strand):  ref base T (12/21);  new base C (50/62);  total (63/83)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 148 total aligned reads displayed.

CGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTAT  >  NC_000913/1913176‑1913371
                                                                                                 |                                                                                                  
cgcgTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTa                                                                                                  <  2:4088981/100‑1 (MQ=255)
  cgTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAAc                                                                                                >  2:3625436/1‑100 (MQ=255)
   gTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGTATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAAcg                                                                                               <  2:754833/100‑1 (MQ=255)
    ttATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAAcgc                                                                                              <  2:351107/100‑1 (MQ=255)
    ttATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAAcgn                                                                                              <  2:2152838/100‑2 (MQ=255)
    ttATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAAcgc                                                                                              <  1:2612809/100‑1 (MQ=255)
    ttATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAAcgc                                                                                              <  1:826162/100‑1 (MQ=255)
    ttATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGCCGTTACGCCTTTACGTTGCGTACTCCCGCCGCTAAcgc                                                                                              <  2:2567296/100‑1 (MQ=255)
    tcttCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAAcac                                                                                              <  1:2815923/97‑3 (MQ=255)
     tATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAAcgcg                                                                                             <  2:474418/100‑1 (MQ=255)
     tATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAAcgcg                                                                                             <  1:47395/100‑1 (MQ=255)
      ntCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGa                                                                                            >  1:2232120/2‑100 (MQ=255)
       tCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAAcgcaga                                                                                           >  2:2815907/1‑97 (MQ=255)
       tCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGAt                                                                                           <  1:2400207/100‑1 (MQ=255)
        cTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGCTGATGTCTGGCGTTACGCCTTTACGTTGCTTACTGCTTCCGTTAACGCGa                                                                                            >  2:871757/1‑98 (MQ=255)
         ttCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTACCGCGATAg                                                                                         >  2:1244944/1‑100 (MQ=255)
         ttCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAg                                                                                         <  1:177396/100‑1 (MQ=255)
          tCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCATCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGa                                                                                        >  1:2945764/1‑100 (MQ=255)
            gAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAAt                                                                                      >  1:1101023/1‑100 (MQ=255)
             aaTTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAAt                                                                                      <  1:2352448/99‑1 (MQ=255)
                 tctcACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTg                                                                                 <  2:2395318/100‑1 (MQ=255)
                 tctcACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTg                                                                                 <  1:863262/100‑1 (MQ=255)
                  ctcACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTgg                                                                                >  2:672977/1‑100 (MQ=255)
                  ctcACCCGTTTCCGCTTCTTCATTACCCGTCGGCATGATGATGTCTTGCGTTACACCTTTACGTTGCGTACTGCCGCCGGGAACGCGATAGAATTTCTgg                                                                                >  2:4158863/1‑100 (MQ=255)
                    cACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGAt                                                                              <  1:1782534/100‑1 (MQ=255)
                    cACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGAt                                                                              <  1:1002586/100‑1 (MQ=255)
                    cACCCGTTTCCGTTTCTTCATTACCCGTAGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCAGCTGACGCGATACAATTTCt                                                                                  >  2:309660/1‑96 (MQ=255)
                     aCCCGTTTCCTTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTGCTGCCGCCGCTAACGCGATAGAATTTCTGGATc                                                                             <  2:2735827/100‑1 (MQ=255)
                     aCCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATc                                                                             <  1:1732273/100‑1 (MQ=255)
                      cccGTTTCCGTTTCTTCATTACCCGTCTGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCg                                                                            <  2:1353739/100‑1 (MQ=255)
                      cccGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCg                                                                            <  1:2338526/100‑1 (MQ=255)
                      cccGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCg                                                                            >  1:2912567/1‑100 (MQ=255)
                      cccGTTTCCGTTTCTGCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTATTGCCGCCGCTAACGCGATAGAATTTCTGGATCg                                                                            <  1:4114465/100‑1 (MQ=255)
                        cgtttccgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCgtg                                                                          <  2:1985351/100‑1 (MQ=255)
                         gtttccgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCgtgt                                                                         >  1:4003730/1‑100 (MQ=255)
                          tttccgtttcTTCATTTCCCGTCTGCATGTTTATTTCTTGCTTTACGCCTTTACGTTGCTTACTTCCGCCGCTTATGCGATAGAATTTCTGGCTct                                                                            <  2:2787177/96‑2 (MQ=255)
                           ttccgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTAc                                                                       <  2:4095453/100‑1 (MQ=255)
                           ttccgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTAc                                                                       >  2:4146048/1‑100 (MQ=255)
                              cgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGc                                                                    >  1:3320977/1‑100 (MQ=255)
                              cgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGc                                                                    >  1:1504376/1‑100 (MQ=255)
                              cgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGc                                                                    >  1:1503033/1‑100 (MQ=255)
                               gtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGca                                                                   <  1:1337987/100‑1 (MQ=255)
                                 ttcttcATTACCCGTCTGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCTGCTAACGCGATAGAATTTCTGGATCGTGTACTGcac                                                                  <  2:2876540/99‑1 (MQ=255)
                                 ttcttcATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGcacc                                                                 <  2:3960285/100‑2 (MQ=255)
                                 ttcttcATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGcaca                                                                 >  1:3934872/1‑100 (MQ=255)
                                 ttcttcATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGcaca                                                                 >  1:2313872/1‑100 (MQ=255)
                                   cttcATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGa                                                               >  1:494321/1‑100 (MQ=255)
                                     tcATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTAATGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGGAc                                                             >  1:2861327/1‑100 (MQ=255)
                                      cATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAAcc                                                            <  1:992559/100‑1 (MQ=255)
                                         tACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAg                                                         <  2:3678491/100‑1 (MQ=255)
                                          aCCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAgc                                                        >  1:3813927/1‑100 (MQ=255)
                                               tCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTgg                                                   <  2:2118684/100‑1 (MQ=255)
                                                cGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGc                                                  <  2:579787/100‑1 (MQ=255)
                                                cGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGc                                                  <  1:3790389/100‑1 (MQ=255)
                                                cGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGCTCGTGTACTGCACAGAACCCAGCGCTGGc                                                  >  1:2011610/1‑100 (MQ=255)
                                                cGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGc                                                  <  1:110750/100‑1 (MQ=255)
                                                 ggCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGcc                                                 >  1:905698/1‑100 (MQ=255)
                                                  gCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGGAATTTTGGATCGTGTACTGGACAGAACACAGCGCTGGACa                                                >  2:3124980/1‑100 (MQ=255)
                                                     tgatgatgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTc                                             <  2:3015175/100‑1 (MQ=255)
                                                     tgatgatgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTc                                             <  2:1617700/100‑1 (MQ=255)
                                                     tgatgatgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTc                                             <  1:2384387/100‑1 (MQ=255)
                                                     tgatgatgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTc                                             <  2:1737438/100‑1 (MQ=255)
                                                      gatgatgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCa                                            <  2:1534146/100‑1 (MQ=255)
                                                      gatgatgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCa                                            >  1:2512156/1‑100 (MQ=255)
                                                         gatgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCa                                            <  1:3857755/97‑1 (MQ=255)
                                                           tgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACg                                       <  1:3490120/100‑1 (MQ=255)
                                                           tgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATACAGGACg                                       <  1:2191025/100‑1 (MQ=255)
                                                             tCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTa                                     >  2:3515208/1‑100 (MQ=255)
                                                               tagCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAAc                                   >  1:2811557/3‑100 (MQ=255)
                                                               tGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAAc                                   <  2:1896333/100‑1 (MQ=255)
                                                                  cGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATc                                >  1:664260/1‑100 (MQ=255)
                                                                   gTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCt                               >  1:3176598/1‑100 (MQ=255)
                                                                   gTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCt                               >  2:2606277/1‑100 (MQ=255)
                                                                   gTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCt                               <  1:3831543/100‑1 (MQ=255)
                                                                   gTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCt                               <  1:1188894/100‑1 (MQ=255)
                                                                      aCGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGAt                            <  1:2649491/100‑1 (MQ=255)
                                                                      aCGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGAt                            >  1:407749/1‑100 (MQ=255)
                                                                      aCGCCGTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGAt                            <  2:863997/100‑1 (MQ=255)
                                                                       cGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATc                           <  2:2110589/100‑1 (MQ=255)
                                                                        gCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCg                          <  2:2445149/100‑1 (MQ=255)
                                                                        gCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCg                          <  1:2525031/100‑1 (MQ=255)
                                                                         ccTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGt                         >  2:1920820/1‑100 (MQ=255)
                                                                              aCGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATa                    >  2:3146269/1‑100 (MQ=255)
                                                                               cGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATAc                   <  1:1852777/100‑1 (MQ=255)
                                                                               cGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATAc                   <  1:3204930/100‑1 (MQ=255)
                                                                                gTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACg                  <  1:3529791/100‑1 (MQ=255)
                                                                                 ttGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACgg                 <  1:998656/100‑1 (MQ=255)
                                                                                  tGCGTACTGCCGCTGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACACAACCCAGCGATGGCCATTCAGGACGTAACATCTGATCGTAAATACGGt                <  2:2059919/100‑1 (MQ=255)
                                                                                   gCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGtt               >  1:2237837/1‑100 (MQ=255)
                                                                                      tACTGCCGCCGCTAACGCGATAGACTTTCTGGATCGTGTACCGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCCTAAACACgggt               >  2:3075531/1‑95 (MQ=255)
                                                                                      tACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCaa            <  2:3690878/100‑1 (MQ=255)
                                                                                       aCTGCGGCCGCTAACGCGCTAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAAt           <  2:1927042/100‑1 (MQ=255)
                                                                                          gccgccGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGaa        >  2:2174663/1‑100 (MQ=255)
                                                                                            cgccgcTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACg      >  1:3328326/1‑100 (MQ=255)
                                                                                             gccgCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACgg     <  2:321285/100‑1 (MQ=255)
                                                                                             gccgCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACgg     <  1:625197/100‑1 (MQ=255)
                                                                                             gccgCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACgg     >  1:1736900/1‑100 (MQ=255)
                                                                                             gccgCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACg      <  2:3738829/99‑1 (MQ=255)
                                                                                             gccgCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACg      >  2:3540297/1‑99 (MQ=255)
                                                                                                gTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTAt  <  2:3217783/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
CGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTAT  >  NC_000913/1913176‑1913371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: