Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,925,214 T→C 72.3% I36T (ATC→ACC)  holE → DNA polymerase III, theta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,925,2140TC72.3% 232.9 / 91.2 141I36T (ATC→ACC) holEDNA polymerase III, theta subunit
Reads supporting (aligned to +/- strand):  ref base T (12/27);  new base C (47/54);  total (60/81)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

GAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACG  >  NC_000913/1925116‑1925310
                                                                                                  |                                                                                                
  aTCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGc                                                                                               <  1:272638/100‑1 (MQ=255)
  aTCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGc                                                                                               >  2:2198650/1‑100 (MQ=255)
    cTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTg                                                                                             <  2:297201/100‑1 (MQ=255)
     tGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGa                                                                                            <  1:3464802/100‑1 (MQ=255)
     tGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGa                                                                                            <  2:2150067/100‑1 (MQ=255)
       gCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAg                                                                                          <  1:674548/100‑1 (MQ=255)
        cTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGc                                                                                         <  1:1798568/100‑1 (MQ=255)
        cTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGc                                                                                         >  2:2780336/1‑100 (MQ=255)
        cTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGc                                                                                         >  1:2479025/1‑100 (MQ=255)
        cTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGc                                                                                         <  2:2451697/100‑1 (MQ=255)
        cTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGc                                                                                         >  2:1274838/1‑100 (MQ=255)
          aaaCTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCgg                                                                                       >  1:1077242/1‑100 (MQ=255)
           aaCTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATTCCGGTGACCGCTGAAGCGGt                                                                                      <  2:2544449/100‑1 (MQ=255)
            aCTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGtt                                                                                     <  1:3213066/100‑1 (MQ=255)
            aCTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGtt                                                                                     <  1:2480894/100‑1 (MQ=255)
             cTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGtt                                                                                     <  2:1200101/99‑1 (MQ=255)
               ggATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTTCAGCGGtcacc                                                                                  <  1:383283/100‑5 (MQ=255)
               ggATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTTCAGCGGtcacc                                                                                  <  1:2759625/100‑5 (MQ=255)
               ggATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGaa                                                                                  >  1:3099664/1‑100 (MQ=255)
                gATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAAc                                                                                 >  2:1107776/1‑100 (MQ=255)
                gATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAAc                                                                                 <  1:1940963/100‑1 (MQ=255)
                gATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTAAAATATGCCGGTGACAGCTGAAGCGGTTGaa                                                                                  >  2:689422/1‑99 (MQ=255)
                 aTCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACg                                                                                >  1:3235912/1‑100 (MQ=255)
                 aTCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACg                                                                                >  2:2365736/1‑100 (MQ=255)
                 aTCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACg                                                                                <  2:3216562/100‑1 (MQ=255)
                   cAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACTCTACAATATGCCGGTGACCGCTGAGGCGGTTGAACGTg                                                                              <  2:2575531/100‑1 (MQ=255)
                   cAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTg                                                                              >  2:1225597/1‑100 (MQ=255)
                    aaaCAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTg                                                                              <  2:777289/99‑1 (MQ=255)
                    aaaCAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGa                                                                             >  2:174247/1‑100 (MQ=255)
                    aaaCAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGa                                                                             <  1:1518470/100‑1 (MQ=255)
                    aaaCAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGa                                                                             <  1:3806074/100‑1 (MQ=255)
                     aaCAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGaa                                                                            <  1:3563180/100‑1 (MQ=255)
                       cAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAAc                                                                           <  2:2830306/99‑1 (MQ=255)
                         gAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGc                                                                        <  1:3682832/100‑1 (MQ=255)
                           aaTGGCTAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGAATTTAAAGAACGCTACAATATGGCGGTGAGCGCTGAACCGGTTGACCGTGCAc                                                                           >  2:49351/1‑95 (MQ=255)
                           aaTGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCt                                                                      >  1:4045133/1‑100 (MQ=255)
                              gggtAAAGTGAATGTCGAGTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGa                                                                    <  2:3200054/96‑1 (MQ=255)
                               gATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAAc                                                                  <  1:654245/100‑1 (MQ=255)
                                    aGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACAtttn                                                             <  1:117179/100‑2 (MQ=255)
                                      tGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTgcg                                                           <  2:3717021/100‑1 (MQ=255)
                                      tGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTgcg                                                           <  2:2664788/100‑1 (MQ=255)
                                       gAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTgcgc                                                          <  1:1981340/100‑1 (MQ=255)
                                       gAATGGCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAGCGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTgcgc                                                          <  2:3227726/100‑1 (MQ=255)
                                        aaTGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCa                                                         <  2:3411110/100‑1 (MQ=255)
                                           gTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCt                                                      >  1:2947821/1‑100 (MQ=255)
                                              gATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGt                                                   >  1:3350676/1‑100 (MQ=255)
                                              gATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGt                                                   <  1:4071230/100‑1 (MQ=255)
                                              gATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGt                                                   >  1:458863/1‑100 (MQ=255)
                                               aTTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGtt                                                  >  1:1161864/1‑100 (MQ=255)
                                               aTTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGtt                                                  <  1:2631284/100‑1 (MQ=255)
                                                tttGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGttt                                                 >  1:3988820/1‑100 (MQ=255)
                                                 ttGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCAGGTGACCGCTGAAGCGGTTGCACGTGAACAGCCTGAACATTTGCGCAGCTCGTTTc                                                >  2:1596305/1‑100 (MQ=255)
                                                    gcggcggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTcgcg                                             <  1:1840471/100‑1 (MQ=255)
                                                    gcggcggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTcgcg                                             <  1:2349105/100‑1 (MQ=255)
                                                     cggcggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGa                                            >  1:1869346/1‑100 (MQ=255)
                                                     cggcggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGa                                            <  2:3729934/100‑1 (MQ=255)
                                                      ggcggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAg                                           >  2:247551/1‑100 (MQ=255)
                                                      ggcggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAg                                           <  2:2203430/100‑1 (MQ=255)
                                                        cggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCg                                         >  2:3084621/1‑100 (MQ=255)
                                                         ggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGCACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCgg                                        <  2:2232640/100‑1 (MQ=255)
                                                         ggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCgg                                        >  1:347619/1‑100 (MQ=255)
                                                           ccGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCt                                      >  1:3718502/1‑100 (MQ=255)
                                                             ggggTGGCATTTAAAGATCGCTACAATATGCCGGTGACCGCTGAGGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCt                                      <  2:1015843/98‑1 (MQ=255)
                                                             ggggTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTa                                    <  1:1648171/100‑1 (MQ=255)
                                                              gggTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTAt                                   <  1:2229059/100‑1 (MQ=255)
                                                              gggTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTAt                                   <  2:2366738/100‑1 (MQ=255)
                                                               ggTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTAtt                                  >  2:1767379/1‑100 (MQ=255)
                                                               ggTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTAtt                                  <  2:3986798/100‑1 (MQ=255)
                                                                gTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTg                                 >  1:2969482/1‑100 (MQ=255)
                                                                 ttgtaTTTAAAGAATGCTACAATATGCCGTTGATTGCTGAAGCGGTTGAACGTTAATAGTCTTAATATTTTCTCAGTTTGTTTCGCGAGTGGCTTATTg                                 <  2:1953131/95‑1 (MQ=255)
                                                                 tGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGc                                >  2:1429830/1‑100 (MQ=255)
                                                                   gCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGccc                              >  1:1288087/1‑100 (MQ=255)
                                                                    cATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCa                             >  1:3052822/1‑100 (MQ=255)
                                                                    cATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCa                             >  1:1437013/1‑100 (MQ=255)
                                                                     aTTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCAc                            >  2:1543086/1‑100 (MQ=255)
                                                                     aTTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCAc                            <  1:1911537/100‑1 (MQ=255)
                                                                       ttAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCg                          >  2:823016/1‑100 (MQ=255)
                                                                        tAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGt                         >  2:307017/1‑100 (MQ=255)
                                                                           aGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTg                      <  1:4002643/100‑1 (MQ=255)
                                                                           aGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTg                      <  1:175244/100‑1 (MQ=255)
                                                                           aGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTg                      <  2:641259/100‑1 (MQ=255)
                                                                           aGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTg                      <  1:440941/100‑1 (MQ=255)
                                                                            gAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTg                      <  1:3695985/99‑1 (MQ=255)
                                                                              aCGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCt                   >  1:2344439/1‑100 (MQ=255)
                                                                                gCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTc                 <  2:2601883/100‑1 (MQ=255)
                                                                                 cTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCg                >  1:3726277/1‑100 (MQ=255)
                                                                                  tACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCgg               <  1:2242894/100‑1 (MQ=255)
                                                                                  tACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCgg               <  1:3829973/100‑1 (MQ=255)
                                                                                   aCAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTc                 >  1:2300512/1‑97 (MQ=255)
                                                                                   aCAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGt              >  1:1597571/1‑100 (MQ=255)
                                                                                    cAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTc             <  2:3440871/100‑1 (MQ=255)
                                                                                    cAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTc             <  2:1458899/100‑1 (MQ=255)
                                                                                     aaTATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCa            <  2:893952/100‑1 (MQ=255)
                                                                                      atatGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCaa           <  1:588803/100‑1 (MQ=255)
                                                                                       tatGCCTGGGACCGCTGAAACGTTTGAACGTGAGCAGCGCGAAAATTTGCGCTGCGGTGTTCGCGAGCGGCTTATTGGCCGCCTTTTGGCTTAggg              <  2:728098/96‑2 (MQ=255)
                                                                                       tatGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAAt          <  2:3774216/100‑1 (MQ=255)
                                                                                          gCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTg       >  1:4146650/1‑100 (MQ=255)
                                                                                          gCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTg       <  2:2968868/100‑1 (MQ=255)
                                                                                          gCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTg       >  2:213566/1‑100 (MQ=255)
                                                                                                gATCGCTGACGCGGTTGAAAGTAAACATCTTAAAAATTTGCGCAGCTGGTTTCGCAAGCGGCATATTGCCCACAGTTTGACTTCGGTCAATCTGTCACg  >  2:3654714/1‑99 (MQ=255)
                                                                                                  |                                                                                                
GAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACG  >  NC_000913/1925116‑1925310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: