Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,946,955 G→A 18.0% A141V (GCC→GTC)  ruvC ← component of RuvABC resolvasome, endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,946,9550GA18.0% 301.6 / 48.1 133A141V (GCC→GTC) ruvCcomponent of RuvABC resolvasome, endonuclease
Reads supporting (aligned to +/- strand):  ref base G (59/49);  new base A (9/15);  total (68/65)
Fisher's exact test for biased strand distribution p-value = 1.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 141 total aligned reads displayed.

AACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGTTTGC  >  NC_000913/1946857‑1947053
                                                                                                  |                                                                                                    
aaCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATcggc                                                                                                     >  2:203542/1‑100 (MQ=255)
  cGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATcggcgg                                                                                                   <  1:2291003/100‑1 (MQ=255)
  cGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATcggcgg                                                                                                   <  1:553546/100‑1 (MQ=255)
  cGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATcggcgg                                                                                                   >  1:1677182/1‑100 (MQ=255)
  cGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATcgacgg                                                                                                   <  2:382673/100‑1 (MQ=255)
    cAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCa                                                                                                 >  2:187080/1‑100 (MQ=255)
          cccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGcc                                                                                           <  2:2948290/100‑1 (MQ=255)
                cGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGa                                                                                     >  1:775133/1‑100 (MQ=255)
                cGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATACGCCTGTgg                                                                                      >  1:3052176/1‑99 (MQ=255)
                cGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATccacaggcg                                                                                       <  2:3284833/98‑8 (MQ=255)
                 gCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGAt                                                                                    <  1:586189/100‑1 (MQ=255)
                     ggTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGc                                                                                <  2:1698454/100‑1 (MQ=255)
                     ggTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGc                                                                                <  2:1698031/100‑1 (MQ=255)
                     ggTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGc                                                                                <  1:2025518/100‑1 (MQ=255)
                       ttCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCgg                                                                              >  1:2875925/1‑100 (MQ=255)
                       ttCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCgg                                                                              <  2:803166/100‑1 (MQ=255)
                       ttCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCgg                                                                              <  1:2405575/100‑1 (MQ=255)
                        tCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCggg                                                                             <  1:3373714/100‑1 (MQ=255)
                        tCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCggg                                                                             <  2:2674469/100‑1 (MQ=255)
                         cAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGc                                                                            <  1:3355474/100‑1 (MQ=255)
                           gCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCa                                                                           >  2:3341139/1‑99 (MQ=255)
                            ccGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGt                                                                         >  1:3156986/1‑100 (MQ=255)
                              gcgATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGttt                                                                       <  2:1808883/100‑1 (MQ=255)
                              gcgATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGttt                                                                       >  1:703245/1‑100 (MQ=255)
                                gatctgCTCATCTGCATCGCTTTCGCACTAACGTGGCATTGCGTGATAACATTCGCTAGCTCATCGGCGGCATCCGTCTGTGGATTATCGGTCAGTTTCa                                                                     <  2:3633275/95‑1 (MQ=255)
                                gATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCa                                                                     <  1:3723390/100‑1 (MQ=255)
                                  ttCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGc                                                                   <  1:3160324/100‑1 (MQ=255)
                                    cGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCaa                                                                 <  1:2080677/100‑1 (MQ=255)
                                     gCTCTTCTGCATCGCATTCTGACTAACGAGGCCGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCGGCAGCCTGTGGATTAGCGGGCAGTTTCAGCAAg                                                                >  2:1893814/1‑100 (MQ=255)
                                     gCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAg                                                                >  2:3035558/1‑100 (MQ=255)
                                     gCTCATCTGCATCACATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAg                                                                <  1:900381/100‑1 (MQ=255)
                                       tCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGt                                                              >  2:697481/1‑100 (MQ=255)
                                        cATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTg                                                             >  1:295020/1‑100 (MQ=255)
                                         aTCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGc                                                            <  2:3100421/100‑1 (MQ=255)
                                          tCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCg                                                           >  2:4041505/1‑100 (MQ=255)
                                          tCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCg                                                           >  1:154897/1‑100 (MQ=255)
                                          tCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCg                                                           >  2:2154707/1‑100 (MQ=255)
                                           cTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCgg                                                          <  1:2020558/100‑1 (MQ=255)
                                           cTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCgg                                                          <  1:216618/100‑1 (MQ=255)
                                            tGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATAGGCGGCATCCGCCTGTGGATTAGCGGGAAGTTTCAGCAAGGTGCgg                                                          >  1:3866888/1‑99 (MQ=255)
                                             gCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGAc                                                        >  2:797881/1‑100 (MQ=255)
                                             gCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGAc                                                        <  1:752521/100‑1 (MQ=255)
                                             gCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGAc                                                        >  1:2688877/1‑100 (MQ=255)
                                             gCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGAc                                                        >  1:2962187/1‑100 (MQ=255)
                                             gCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGAc                                                        >  2:108360/1‑100 (MQ=255)
                                              cATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGAcc                                                       >  2:2575183/1‑100 (MQ=255)
                                               aTCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCa                                                      >  1:621135/1‑100 (MQ=255)
                                                 cGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCata                                                    <  1:1276467/100‑1 (MQ=255)
                                                  gCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCatat                                                   >  1:3855726/1‑100 (MQ=255)
                                                  gCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCatat                                                   >  1:1367432/1‑100 (MQ=255)
                                                    aTTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATAtg‑‑c                                               <  1:1025376/100‑1 (MQ=255)
                                                    aTTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATAtg‑‑c                                               >  1:2149381/1‑100 (MQ=255)
                                                       cTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATAtg‑‑ctgc                                            >  1:1585823/1‑100 (MQ=255)
                                                       cTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATAtg‑‑ctgc                                            <  2:3433158/100‑1 (MQ=255)
                                                        tGACTAACGTGGGAGTGGGTGATAGGCATCGCCTGCGCATCGTCGGCATCCGCCTGTGGAGTCGCGGCCAGTGTCAGGAAGGCGCCGGCCATATC‑‑CTGCa                                           <  2:3588388/100‑1 (MQ=255)
                                                        tGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCa                                           >  1:3107575/1‑100 (MQ=255)
                                                         gACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCAc                                          >  1:1108843/1‑100 (MQ=255)
                                                            tAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTg                                       <  1:1820856/100‑1 (MQ=255)
                                                             aaCGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTgg                                      >  2:3177800/1‑100 (MQ=255)
                                                             aaCGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTgg                                      >  2:2199699/1‑100 (MQ=255)
                                                             aaCGTGGCAGTGAGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTgg                                      >  1:2737414/1‑100 (MQ=255)
                                                               cGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCt                                    <  2:3197328/100‑1 (MQ=255)
                                                                  ggCCGTGCGTAATAGCAATCGCAAGCGCATCGGCGGCATCCGCCTGTGCATTACCCGGCTGTTTCAGTCAGGTCC‑‑AACATATGGCCTGCACCTGGCtt                                   <  2:3478193/98‑1 (MQ=255)
                                                                  ggCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCtttt                                 >  1:157215/1‑100 (MQ=255)
                                                                   ctaGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCttttt                                <  1:1284357/98‑1 (MQ=255)
                                                                     aGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTc                              <  1:2904129/100‑1 (MQ=255)
                                                                     aGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTc                              >  1:1130628/1‑100 (MQ=255)
                                                                      gTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCg                             <  2:2885285/100‑1 (MQ=255)
                                                                      gTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCg                             <  1:2897914/100‑1 (MQ=255)
                                                                      gTGGGTGATAGCAAACGCCAGAGCAACGGCGGCACCCGCCTGTGGCTAAACGAGAAGTCTCAGCAAGACGCCGACCAAAAG‑‑CTGCACCTGGCctttt                                >  2:1192392/1‑97 (MQ=255)
                                                                       tGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCgg                            <  2:3519102/100‑1 (MQ=255)
                                                                       tGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCgg                            <  2:3961470/100‑1 (MQ=255)
                                                                        gggTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGc                           <  1:1554691/100‑1 (MQ=255)
                                                                        gggTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGc                           <  1:2055299/100‑1 (MQ=255)
                                                                        gggTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGc                           >  1:207818/1‑100 (MQ=255)
                                                                         ggTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTCGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCGGGCTTTTTTCGGc                           >  2:2794278/1‑99 (MQ=255)
                                                                         ggTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCa                          >  2:634860/1‑100 (MQ=255)
                                                                            gATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTg                       >  2:902529/1‑100 (MQ=255)
                                                                            gATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTg                       <  2:1173613/100‑1 (MQ=255)
                                                                              tAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCACGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGcc                     <  2:2854833/100‑1 (MQ=255)
                                                                              tAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGcc                     >  1:3262740/1‑100 (MQ=255)
                                                                               aacAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCa                    >  2:3426077/3‑100 (MQ=255)
                                                                               aGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACGATATG‑‑CGGCACCTGGCTTTTTTCGGCACTGCCa                    >  2:892603/1‑100 (MQ=255)
                                                                                gCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCaa                   <  2:537847/100‑1 (MQ=255)
                                                                                gCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCaa                   >  2:318324/1‑100 (MQ=255)
                                                                                gCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCaa                   >  2:3536828/1‑100 (MQ=255)
                                                                                gCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCaa                   >  1:1820990/1‑100 (MQ=255)
                                                                                 cAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAAt                  <  1:2071419/100‑1 (MQ=255)
                                                                                 cAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAAt                  >  1:3915392/1‑100 (MQ=255)
                                                                                  aaTCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAAta                 <  1:2765415/100‑1 (MQ=255)
                                                                                      gCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAAtaccta             >  1:2955872/1‑100 (MQ=255)
                                                                                       ccAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAAtaccta             <  2:11293/99‑1 (MQ=255)
                                                                                       ccAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCAGTGCCAAtacctac            <  1:2828249/100‑1 (MQ=255)
                                                                                        cAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAAtacctacc           <  2:3458026/100‑1 (MQ=255)
                                                                                         aGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAATACCTacca          <  1:3732622/100‑1 (MQ=255)
                                                                                            gcATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCg       >  2:2402734/1‑100 (MQ=255)
                                                                                              aTCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CGGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGtt     >  1:2338019/1‑100 (MQ=255)
                                                                                               tCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGttt    >  1:2089593/1‑100 (MQ=255)
                                                                                               tCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGttt    <  2:3915362/100‑1 (MQ=255)
                                                                                                cggcggCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGTTTg   <  1:492441/100‑1 (MQ=255)
                                                                                                  |                                                                                                    
AACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATG‑‑CTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGTTTGC  >  NC_000913/1946857‑1947053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: