Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,014,867 A→G 79.1% I547V (ATA→GTA)  fliF → flagellar basal‑body MS‑ring and collar protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,014,8670AG79.1% 226.3 / 49.4 110I547V (ATA→GTA) fliFflagellar basal‑body MS‑ring and collar protein
Reads supporting (aligned to +/- strand):  ref base A (12/11);  new base G (38/48);  total (50/60)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

CGCTAACCAACGTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACC  >  NC_000913/2014770‑2014964
                                                                                                 |                                                                                                 
cgcTAACCAACGTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGgta                                                                                                 <  1:3405149/100‑1 (MQ=255)
 gcTAACCAACGTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGataa                                                                                                >  1:3267909/1‑100 (MQ=255)
  cTAACCAACGTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGgtaaa                                                                                               >  2:29691/1‑100 (MQ=255)
         aCGTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGAt                                                                                        <  1:3702015/100‑1 (MQ=255)
          cGTCTGGGGGCAAAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATc                                                                                       <  1:1448898/100‑1 (MQ=255)
           gTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATc                                                                                       <  1:3617637/99‑1 (MQ=255)
           gTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCa                                                                                      >  1:3490315/1‑100 (MQ=255)
           gTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCa                                                                                      <  2:3269676/100‑1 (MQ=255)
           gTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCa                                                                                      >  1:627874/1‑100 (MQ=255)
            tCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCAt                                                                                     >  2:4166206/1‑100 (MQ=255)
             cTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATg                                                                                    >  2:2094402/1‑100 (MQ=255)
             cTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATg                                                                                    <  2:289152/100‑1 (MQ=255)
             cTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATg                                                                                    <  1:2675955/100‑1 (MQ=255)
              tGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGa                                                                                   <  2:3641809/100‑1 (MQ=255)
              tGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGa                                                                                   <  1:629878/100‑1 (MQ=255)
              tGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGa                                                                                   >  2:208698/1‑100 (MQ=255)
              tGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGa                                                                                   >  1:3232151/1‑100 (MQ=255)
              tGGGAGCATAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCTCTGGTCATTCGCCAGTGGTTAAATAACTATCATg                                                                                    <  2:4126022/99‑1 (MQ=255)
               gggggCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAg                                                                                  <  2:741237/100‑1 (MQ=255)
               gggggCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAg                                                                                  <  1:1951944/100‑1 (MQ=255)
               gggggCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAg                                                                                  <  1:3383102/100‑1 (MQ=255)
               gggggCAGAAGTCATGAGCCAGCGTATCCGTGAAATGACTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAg                                                                                  >  2:2224528/1‑100 (MQ=255)
               gggggCAGAAGTCATGAGCCAGCGTATCCGAGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGATCATTCGCCAGTGGGTAAATAACGATCATGAg                                                                                  <  2:4157650/100‑1 (MQ=255)
                ggggCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGt                                                                                 >  2:901013/1‑100 (MQ=255)
                 gggCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTa                                                                                >  1:3633837/1‑100 (MQ=255)
                    cAGGAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAAcc                                                                             <  2:2200020/100‑1 (MQ=255)
                    cAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAAcc                                                                             >  2:2112519/1‑100 (MQ=255)
                     aGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAAcc                                                                             <  2:3575889/99‑1 (MQ=255)
                      gAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTg                                                                           <  1:1569174/100‑1 (MQ=255)
                      gAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTg                                                                           >  1:1551760/1‑100 (MQ=255)
                        aGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATTATGAGTAACCt                                                                            >  2:4041266/1‑97 (MQ=255)
                        aGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGAc                                                                         <  2:3232130/100‑1 (MQ=255)
                         gTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACa                                                                        <  1:702981/100‑1 (MQ=255)
                         gTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACa                                                                        >  2:2773169/1‑100 (MQ=255)
                         gTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGGCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACa                                                                        <  1:427683/100‑1 (MQ=255)
                          tCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAg                                                                       <  2:3848604/100‑1 (MQ=255)
                           cATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAgg                                                                      >  2:885898/1‑100 (MQ=255)
                             tGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCa                                                                    <  1:1389274/100‑1 (MQ=255)
                               aGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCAcc                                                                  <  2:30142/100‑1 (MQ=255)
                               aGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCAcc                                                                  <  1:240495/100‑1 (MQ=255)
                                gCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCg                                                                 <  2:773186/100‑1 (MQ=255)
                                  cAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGAt                                                               <  1:678947/100‑1 (MQ=255)
                                  cAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCAAt                                                               <  2:3527529/100‑1 (MQ=255)
                                   aGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATa                                                              <  1:3128294/100‑1 (MQ=255)
                                    gCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATaa                                                             <  1:1063278/100‑1 (MQ=255)
                                    gCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATaa                                                             <  2:1051640/100‑1 (MQ=255)
                                     cGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATaaa                                                            <  1:1599875/100‑1 (MQ=255)
                                         tCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCg                                                        <  2:3439710/100‑1 (MQ=255)
                                         tCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCg                                                        <  1:2326224/100‑1 (MQ=255)
                                          ccGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGt                                                       >  2:2885049/1‑100 (MQ=255)
                                           cGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGt                                                       >  1:210461/1‑99 (MQ=255)
                                           cGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTc                                                      <  1:1937606/100‑1 (MQ=255)
                                             tGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCAt                                                    <  1:3612601/100‑1 (MQ=255)
                                              gAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATc                                                   <  2:250595/100‑1 (MQ=255)
                                               aaaTGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATcc                                                  >  1:2988592/1‑100 (MQ=255)
                                                  tGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCctgc                                               <  2:1625840/100‑1 (MQ=255)
                                                   gTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCctgct                                              >  1:2920590/1‑100 (MQ=255)
                                                     cTGAGAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCctgctg                                             <  2:1961995/99‑1 (MQ=255)
                                                       gATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCGGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCtgatg                                          <  1:3443377/100‑1 (MQ=255)
                                                       gATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCtgatg                                          >  1:4135510/1‑100 (MQ=255)
                                                        aTAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAAAGATCATGAGTAGCCTGACAGGCACCGATAATAGCGTCATCCTGCtgatga                                         <  2:3149807/100‑1 (MQ=255)
                                                         tAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGAc                                        >  2:2553041/1‑100 (MQ=255)
                                                         tAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGAc                                        <  1:1875823/100‑1 (MQ=255)
                                                           aCGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGAcc                                       <  2:1133198/99‑1 (MQ=255)
                                                           aCGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCa                                      >  2:3135534/1‑100 (MQ=255)
                                                            cGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCAt                                     >  2:3943022/1‑100 (MQ=255)
                                                             gATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCAtt                                    >  1:232145/1‑100 (MQ=255)
                                                              aTCCGCGCGTTGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCTTCCTGCTGATGACCATTg                                   <  1:3661288/100‑1 (MQ=255)
                                                              aTCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTg                                   >  1:39738/1‑100 (MQ=255)
                                                              aTCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTg                                   >  2:628166/1‑100 (MQ=255)
                                                                 cgcgcgTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCg                                >  1:1916405/1‑100 (MQ=255)
                                                                 cgcgcgTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCg                                >  1:192850/1‑100 (MQ=255)
                                                                  gcgcgTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGa                               <  2:4038708/100‑1 (MQ=255)
                                                                   cgcgTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGaa                              <  1:3893559/100‑1 (MQ=255)
                                                                    gcgTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACTTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAg                             <  1:1911008/100‑1 (MQ=255)
                                                                    gcgTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAg                             <  1:3595837/100‑1 (MQ=255)
                                                                    gcgTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAg                             <  2:1174977/100‑1 (MQ=255)
                                                                     cgTGGTGGCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGa                            <  2:1420492/100‑1 (MQ=255)
                                                                        ggtggCGCTGGTCATTCGCCAGTGGGTAAAGAACGATCATGAGTAACCTGACAGGCACCGAGAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGAcc                          <  2:3600213/99‑1 (MQ=255)
                                                                         gtggCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCgg                        <  2:1019198/100‑1 (MQ=255)
                                                                          tggCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCggg                       >  2:1995783/1‑100 (MQ=255)
                                                                          tggCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCgg                        >  2:2274320/1‑99 (MQ=255)
                                                                           ggCGCTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGggc                      >  1:3071154/1‑100 (MQ=255)
                                                                              ncTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGCAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGggcggc                   >  2:2232165/2‑100 (MQ=255)
                                                                              gcTGGTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGggcggc                   >  1:830654/1‑100 (MQ=255)
                                                                                 ggTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCaga                >  1:846220/1‑100 (MQ=255)
                                                                                  gTCATTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCagag               >  1:2288117/1‑100 (MQ=255)
                                                                                     aTTCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGgtg            <  2:2015583/100‑1 (MQ=255)
                                                                                     aTTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGgtg            >  2:2623604/1‑100 (MQ=255)
                                                                                      ttCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAACAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGgtgt           >  1:243009/1‑100 (MQ=255)
                                                                                       tCGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGtt          <  1:200831/100‑1 (MQ=255)
                                                                                        cGCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTc         >  1:1734753/1‑100 (MQ=255)
                                                                                         gCCAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATACTGCTGATAACCATTGGCGAAAACCGGGCGGCAGAGGTGTTCa        >  2:692833/1‑100 (MQ=255)
                                                                                         gCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGAACAGATAAAAGCGTCATCCTGCTGATGACAATTGGCGAAGACCAAGCACCAGAGGAGTTCa        >  2:3108976/1‑100 (MQ=255)
                                                                                          cccgTGGATAAATAACGATCATGAGTAACCTGACAGGCACCCATAAAAGCGCCATCCTGCTGATGCCCATTGGCGAAGACCGGGCGGCAGAGGCGCTCaa       >  2:2294977/4‑100 (MQ=255)
                                                                                          ccAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCaa       <  1:2642468/100‑1 (MQ=255)
                                                                                           cAGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAg      <  1:3034707/100‑1 (MQ=255)
                                                                                           cAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAg      <  1:2773185/100‑1 (MQ=255)
                                                                                            aGTGGGTAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGc     <  2:2307111/100‑1 (MQ=255)
                                                                                               ggATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCAcc  <  2:1327265/100‑1 (MQ=255)
                                                                                                 |                                                                                                 
CGCTAACCAACGTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACC  >  NC_000913/2014770‑2014964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: