Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,036,066 A→C 78.3% T78P (ACG→CCG)  hchA → glyoxalase III and Hsp31 molecular chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,036,0660AC78.3% 342.8 / 75.2 146T78P (ACG→CCG) hchAglyoxalase III and Hsp31 molecular chaperone
Reads supporting (aligned to +/- strand):  ref base A (8/24);  new base C (52/62);  total (60/86)
Fisher's exact test for biased strand distribution p-value = 4.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

TATCGCGGTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTGGGCTATGC  >  NC_000913/2035967‑2036162
                                                                                                   |                                                                                                
tATCGCGGTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGaaa                                                                                                  >  2:1446811/1‑100 (MQ=255)
   cgcgGTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGt                                                                                               >  1:579249/1‑100 (MQ=255)
    gcgGTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGtt                                                                                              >  2:4089859/1‑100 (MQ=255)
      ggTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTtgc                                                                                            <  2:29007/100‑1 (MQ=255)
      ggTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTtgc                                                                                            >  1:1176002/1‑100 (MQ=255)
          aaCATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGcc                                                                                        >  2:641368/1‑100 (MQ=255)
            cATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGt                                                                                      >  2:2360272/1‑100 (MQ=255)
            cATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGt                                                                                      >  1:870864/1‑100 (MQ=255)
             aTAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACCTTGATGCCGtt                                                                                     <  2:2189959/100‑1 (MQ=255)
              tAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTg                                                                                    <  1:1793688/100‑1 (MQ=255)
              tAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTg                                                                                    <  1:1570817/100‑1 (MQ=255)
              tAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTg                                                                                    >  1:3007867/1‑100 (MQ=255)
              tAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTg                                                                                    <  2:3773768/100‑1 (MQ=255)
              tAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTg                                                                                    <  1:751763/100‑1 (MQ=255)
               ataaTTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGt                                                                                   <  2:2533196/98‑1 (MQ=255)
                aaaTTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTa                                                                                  >  2:1560555/1‑100 (MQ=255)
                aaaTTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTa                                                                                  <  1:3275855/100‑1 (MQ=255)
                aaaTTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTa                                                                                  <  2:1681089/100‑1 (MQ=255)
                aaaTTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTa                                                                                  <  1:2453106/100‑1 (MQ=255)
                aaaTTCTGGTGATCGCCGAGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTa                                                                                  <  2:3202897/100‑1 (MQ=255)
                   ttCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTatca                                                                               <  1:1948428/100‑1 (MQ=255)
                         tGATCGCCGCTGACTAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCAGAATGAAACGTTGCTGCCGTTGTATCATCTCCa                                                                         <  2:2744359/100‑1 (MQ=255)
                         tGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCa                                                                         <  2:800744/100‑1 (MQ=255)
                         tGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCa                                                                         >  1:2783657/1‑100 (MQ=255)
                         tGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCa                                                                         <  2:1250206/100‑1 (MQ=255)
                            tCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCAtgc                                                                      <  1:3215667/100‑1 (MQ=255)
                               ccgcGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCAtgctgc                                                                   >  1:2591511/1‑100 (MQ=255)
                                cgcgGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCa                                                                  <  1:3085837/100‑1 (MQ=255)
                                 gcgGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAg                                                                 <  1:3077759/100‑1 (MQ=255)
                                 gcgGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAg                                                                 <  1:2171657/100‑1 (MQ=255)
                                     aCGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGttt                                                             >  1:1756087/1‑100 (MQ=255)
                                     aCGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGttt                                                             <  1:530757/100‑1 (MQ=255)
                                      cGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGttt                                                             <  2:641974/99‑1 (MQ=255)
                                      cGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttc                                                            <  2:3961645/100‑1 (MQ=255)
                                       gAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcg                                                           <  1:936754/100‑1 (MQ=255)
                                       gAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcg                                                           <  1:1253219/100‑1 (MQ=255)
                                       gAACGTTATTTGCCGACAGATAACGGAAACCTGTTCTCGATCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttc                                                            <  2:2642100/99‑1 (MQ=255)
                                        aaCGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcga                                                          >  1:1036030/1‑100 (MQ=255)
                                        aaCGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcga                                                          <  2:1627806/100‑1 (MQ=255)
                                          cGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcgaat                                                        >  1:740453/1‑100 (MQ=255)
                                          cGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcgaat                                                        <  2:3609477/100‑1 (MQ=255)
                                            ttATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcgaattc                                                      >  2:3033251/1‑100 (MQ=255)
                                             tATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcgaattcg                                                     <  2:3228181/100‑1 (MQ=255)
                                              aTTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcgaattcga                                                    <  1:2307737/100‑1 (MQ=255)
                                              aTTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcgaattcga                                                    <  1:578782/100‑1 (MQ=255)
                                                 tGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGt                                                 <  2:870859/100‑1 (MQ=255)
                                                 tGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGCTTTCGAATTCGAAGt                                                 >  1:764542/1‑100 (MQ=255)
                                                  gCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTg                                                <  1:2990539/100‑1 (MQ=255)
                                                   ccgaccgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTgg                                               <  1:976183/100‑1 (MQ=255)
                                                     gaccgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGATGTGGCg                                             >  2:2773226/1‑100 (MQ=255)
                                                     gaccgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCg                                             <  2:1673490/100‑1 (MQ=255)
                                                       ccgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGAc                                           <  2:3231600/100‑1 (MQ=255)
                                                        cgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGAcc                                          >  1:742155/1‑100 (MQ=255)
                                                         naTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCa                                         >  2:3418404/2‑100 (MQ=255)
                                                         gaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGAc                                           <  2:2874791/98‑1 (MQ=255)
                                                         gaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCa                                         >  2:112173/1‑100 (MQ=255)
                                                           tAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCAtt                                       >  1:1828207/1‑100 (MQ=255)
                                                             aCGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGTTGCAGGTTTCGAATTCGAAGTAGCGGCCATTTc                                     >  2:1969419/1‑100 (MQ=255)
                                                             aCGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTc                                     >  1:3498691/1‑100 (MQ=255)
                                                              cGGAAAACTGTTCTCGACCGTTAACCATCCGATTGACACGTTGCTGCCGTTGTATCATCACCATGCCGCACGTTTCGAATTCGAAGTGGCGACCAttt                                      >  2:1406364/1‑98 (MQ=255)
                                                              cGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTcc                                    >  2:3108808/1‑100 (MQ=255)
                                                              cGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTcc                                    >  2:3551016/1‑100 (MQ=255)
                                                                gAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCACGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCgg                                  <  1:3451320/100‑1 (MQ=255)
                                                                 aaaaCTGTTCTCTACCTGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGTTTTCTAATTCGAAGTGGCGACCATTTCCg                                   <  2:1501229/98‑1 (MQ=255)
                                                                  aaaCTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTc                                <  1:794765/100‑1 (MQ=255)
                                                                   aaCTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCt                               <  1:2232217/100‑1 (MQ=255)
                                                                   aaCTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCt                               <  1:2847889/100‑1 (MQ=255)
                                                                    aCTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtg                              <  1:951414/100‑1 (MQ=255)
                                                                     cTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtga                             <  1:3707270/100‑1 (MQ=255)
                                                                     cTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtga                             >  2:529473/1‑100 (MQ=255)
                                                                      tGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgat                            >  2:1833416/1‑100 (MQ=255)
                                                                       gTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgatg                           <  1:3611924/100‑1 (MQ=255)
                                                                       gTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgatg                           <  1:2045230/100‑1 (MQ=255)
                                                                       gTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgatg                           <  2:2256896/100‑1 (MQ=255)
                                                                       gTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgatg                           <  1:436516/100‑1 (MQ=255)
                                                                        ttCTCGACCGGTAACCATTCTATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgatga                          <  2:4168909/100‑1 (MQ=255)
                                                                        ttCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgatga                          <  1:3866336/100‑1 (MQ=255)
                                                                        ttCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgatga                          <  1:939910/100‑1 (MQ=255)
                                                                         tctcGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGAc                         >  1:2697327/1‑100 (MQ=255)
                                                                         tctcGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGAc                         >  1:3760823/1‑100 (MQ=255)
                                                                         tctcGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGAc                         <  1:1969175/100‑1 (MQ=255)
                                                                         tctcGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGAc                         >  2:3339117/1‑100 (MQ=255)
                                                                             gACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAg                     <  1:3621572/100‑1 (MQ=255)
                                                                               ccGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGtt                   <  1:2532664/100‑1 (MQ=255)
                                                                                cGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGttt                  <  2:3038067/100‑1 (MQ=255)
                                                                                cGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGttt                  >  1:2111286/1‑100 (MQ=255)
                                                                                 ggTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTg                 <  2:1092839/100‑1 (MQ=255)
                                                                                 ggTAACCATCCGATTGAAACGTTGCTGCCGTTATATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTg                 >  1:1827472/1‑100 (MQ=255)
                                                                                   tAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGaa               <  1:3173783/100‑1 (MQ=255)
                                                                                     aCCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAAt              >  2:1746655/1‑99 (MQ=255)
                                                                                      ccATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATAc            <  1:2515486/100‑1 (MQ=255)
                                                                                      ccATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATAc            >  1:3862282/1‑100 (MQ=255)
                                                                                      ccATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATAc            >  1:3202923/1‑100 (MQ=255)
                                                                                        aTCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTg          >  1:3499976/1‑100 (MQ=255)
                                                                                        aTCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTg          >  1:1569815/1‑100 (MQ=255)
                                                                                         tCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTgg         <  1:2661891/100‑1 (MQ=255)
                                                                                             aTTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTGGGCTa     >  1:1061576/1‑100 (MQ=255)
                                                                                               tGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTGGGCTATg   <  1:837852/100‑1 (MQ=255)
                                                                                                gAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTGGGCTATGc  >  2:1879059/1‑100 (MQ=255)
                                                                                                gAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATAACCAAGTTTGAATACTGGGCTATGc  >  1:1033559/1‑100 (MQ=255)
                                                                                                   |                                                                                                
TATCGCGGTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTGGGCTATGC  >  NC_000913/2035967‑2036162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: