Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,808,281 (A)5→4 coding (486/861 nt) yniA → fructosamine kinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,808,2770A.96.2% 339.0 / 6.4 78coding (482/861 nt)yniAfructosamine kinase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base . (31/44);  total (31/47)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

ACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCCTTCTCTGTTGCACGGC  >  NC_000913/1808178‑1808371
                                                                                                   |                                                                                              
aCGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGa                                                                                                >  2:1552564/1‑100 (MQ=255)
aCGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGa                                                                                                <  1:30498/100‑1 (MQ=255)
 cgcgCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGaa                                                                                               >  2:151582/1‑100 (MQ=255)
 cgcgCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGaa                                                                                               >  1:507745/1‑100 (MQ=255)
   cgcTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGaaaa                                                                                             >  2:131641/1‑100 (MQ=255)
     ctctCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGaaaagg                                                                                           >  2:1307981/1‑98 (MQ=255)
     ctctCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGaaaagg                                                                                           <  2:606225/100‑3 (MQ=255)
      tctcCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAggg                                                                                          <  2:1646745/100‑1 (MQ=255)
       ctcCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGaaaaggga                                                                                         >  1:1036708/1‑96 (MQ=255)
         ccACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATc                                                                                      >  2:868962/1‑100 (MQ=255)
         ccACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATc                                                                                      >  1:148056/1‑100 (MQ=255)
         ccACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATc                                                                                      >  2:524709/1‑100 (MQ=255)
          cacaACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCg                                                                                     >  1:1601273/1‑100 (MQ=255)
           acaACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGc                                                                                    <  2:507744/100‑1 (MQ=255)
              aCTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCttt                                                                                 >  1:476819/1‑100 (MQ=255)
               cTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTc                                                                                <  2:1253504/100‑1 (MQ=255)
               cTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTc                                                                                >  2:491978/1‑100 (MQ=255)
                tCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCg                                                                               <  1:767628/100‑1 (MQ=255)
                 ccACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCgg                                                                              >  2:980299/1‑100 (MQ=255)
                 ccACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCgg                                                                              <  1:524710/100‑1 (MQ=255)
                  cacaGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGc                                                                             <  1:1774619/100‑1 (MQ=255)
                  cacaGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGc                                                                             <  2:390895/100‑1 (MQ=255)
                  cacaGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCCGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGc                                                                             <  2:562549/100‑1 (MQ=255)
                     aGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAat                                                                          <  2:1032307/100‑1 (MQ=255)
                      gCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAata                                                                         <  2:1188109/100‑1 (MQ=255)
                            cacCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGAc                                                                    >  2:1090888/1‑99 (MQ=255)
                             acCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGAc                                                                    <  2:589906/98‑1 (MQ=255)
                             acCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGc                                                                  <  2:2010337/100‑1 (MQ=255)
                              ccTGGCAACGTCGCTGGTCAACGTTTTTTTCTTAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGcc                                                                 >  2:1193974/1‑100 (MQ=255)
                              ccTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGcc                                                                 >  1:1720089/1‑100 (MQ=255)
                               cTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCa                                                                >  1:1706846/1‑100 (MQ=255)
                                 ggCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCAtc                                                              >  1:1624714/1‑100 (MQ=255)
                                 ggCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCAtc                                                              >  2:183026/1‑100 (MQ=255)
                                  gCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCAtcg                                                             >  1:450375/1‑100 (MQ=255)
                                  gCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCAtcg                                                             <  1:1045622/100‑1 (MQ=255)
                                  gCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCAtc                                                              >  2:2026500/1‑99 (MQ=255)
                                   cAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCAtcgt                                                            >  1:1623036/1‑100 (MQ=255)
                                       gTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAg                                                        <  2:476818/100‑1 (MQ=255)
                                       gTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAg                                                        >  1:1168534/1‑100 (MQ=255)
                                       gTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAg                                                        >  1:1496033/1‑100 (MQ=255)
                                        tCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGc                                                       <  2:1844899/100‑1 (MQ=255)
                                        tCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGc                                                       <  2:1052300/100‑1 (MQ=255)
                                         cGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACTCCATCGTCGAgta                                                      <  1:1382931/100‑3 (MQ=255)
                                            tGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCatat                                                   <  2:1720076/100‑1 (MQ=255)
                                             ggTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATAtt                                                  >  1:939252/1‑100 (MQ=255)
                                              gTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTc                                                 <  1:1718677/100‑1 (MQ=255)
                                                cAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTcag                                               <  2:1753194/100‑1 (MQ=255)
                                                         ttGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTg                                      <  2:2033371/100‑1 (MQ=255)
                                                          tGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTgg                                     <  1:1307989/100‑1 (MQ=255)
                                                           gCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGc                                    >  1:1821014/1‑100 (MQ=255)
                                                           gCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGc                                    >  1:1146133/1‑100 (MQ=255)
                                                            cTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGcc                                   <  1:868965/100‑1 (MQ=255)
                                                            cTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGcc                                   <  1:1440182/100‑1 (MQ=255)
                                                              gAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTc                                 >  1:100259/1‑100 (MQ=255)
                                                              gAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTc                                 <  1:1193980/100‑1 (MQ=255)
                                                              gAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTc                                 <  1:964347/100‑1 (MQ=255)
                                                                acaacGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTcac                               <  2:526570/100‑1 (MQ=255)
                                                                acaacGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTcac                               <  2:995537/100‑1 (MQ=255)
                                                                   acGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATc                            >  1:1350524/1‑100 (MQ=255)
                                                                    cGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATc                            <  2:669890/100‑1 (MQ=255)
                                                                    cGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCa                           >  2:926171/1‑100 (MQ=255)
                                                                    cGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCCGGCCTCACATCa                           <  1:1337791/100‑1 (MQ=255)
                                                                      gATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAAc                         <  1:183027/100‑1 (MQ=255)
                                                                        ttGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCg                       <  2:152213/100‑1 (MQ=255)
                                                                        ttGGCTGGCAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCg                       <  1:1286923/100‑1 (MQ=255)
                                                                          tcttccgatctGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCa                     <  1:991180/90‑1 (MQ=255)
                                                                          ggctggcAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCa                     >  1:895706/1‑100 (MQ=255)
                                                                          ggctggcAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCa                     >  1:1771850/1‑100 (MQ=255)
                                                                           gctggcAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAg                    <  1:980303/100‑1 (MQ=255)
                                                                           gctggcAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAg                    <  1:524289/100‑1 (MQ=255)
                                                                            ctggcAGTTGGAACTGGCTGCAGAAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAg                    <  1:1552574/100‑1 (MQ=255)
                                                                            ctggcAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGc                   >  1:406056/1‑100 (MQ=255)
                                                                             tggcAGTTGGAACTGGCTGCAGAAAAGGTCTCGCTTTCTGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGcc                  <  1:415856/100‑1 (MQ=255)
                                                                             tggcAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGcc                  <  2:1820998/100‑1 (MQ=255)
                                                                             tggcAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATACTCAGCAGCGTCTGGCCTCACATCAACCGCAGcc                  <  2:1350515/100‑1 (MQ=255)
                                                                                cAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCCTtc               <  2:1987259/100‑1 (MQ=255)
                                                                                cAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCCTtc               <  2:1442007/100‑1 (MQ=255)
                                                                                cAGTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCCTtc               <  2:943640/100‑1 (MQ=255)
                                                                                  gTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGCGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCCTtctc             <  2:1706833/100‑1 (MQ=255)
                                                                                  gTTGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCCTtctc             >  1:37924/1‑100 (MQ=255)
                                                                                   ttGGAACTGGCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCCTTctct            <  1:131642/100‑1 (MQ=255)
                                                                                   ttGGAAATGGCTGCCGAACAGGGATCGCTTTCGGCCATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCTTtc               <  2:678295/97‑1 (MQ=255)
                                                                                           ggCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCCTTCTCTGTTGCACg    >  2:2019862/1‑100 (MQ=255)
                                                                                           ggCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCCTTCTCTGTTGCACg    <  2:939248/100‑1 (MQ=255)
                                                                                           ggCTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCCTTCTCTGTTGCACg    >  1:875949/1‑100 (MQ=255)
                                                                                             cTGCAGAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCCTTCTCTGTTGCACGGc  >  1:1693392/1‑100 (MQ=255)
                                                                                                   |                                                                                              
ACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGAACTGGCTGCAGAAAAAGGGATCGCTTTCGGCAATATCGACGCCATCGTCGAGCATATTCAGCAGCGTCTGGCCTCACATCAACCGCAGCCTTCTCTGTTGCACGGC  >  NC_000913/1808178‑1808371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: