| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,563,518 | (A)5→6 | intergenic (+401/‑74) | yffP → / → yffQ | CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,563,513 | 1 | . | A | 95.6% | 234.1 / 3.7 | 68 | intergenic (+396/‑79) | yffP/yffQ | CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base A (37/28); total (38/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑AAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCG > NC_000913/2563414‑2563607 | aGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGt < 1:444630/100‑1 (MQ=255) gTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aa > 2:1046749/1‑100 (MQ=255) gTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aa > 2:580289/1‑100 (MQ=255) tGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaa < 1:1031909/100‑1 (MQ=255) tGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaa > 2:1394505/1‑100 (MQ=255) aTTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaaaaa < 2:1509288/100‑2 (MQ=255) tttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaaaaatg < 1:1263650/100‑4 (MQ=255) tttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaaaaatg < 1:580291/100‑4 (MQ=255) tttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaaaaatg < 1:1441234/100‑4 (MQ=255) gtgATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGaaa > 2:1925898/1‑100 (MQ=255) tGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGt > 1:446310/1‑100 (MQ=255) tGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGt > 1:1774258/1‑100 (MQ=255) gCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTg < 1:992420/100‑1 (MQ=255) aGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTggg < 2:1774244/100‑1 (MQ=255) gATCGCTTTTTCCTTTTAGTCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTTCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGa > 2:818172/1‑100 (MQ=255) gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGa > 2:547369/1‑100 (MQ=255) gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGa > 1:708800/1‑100 (MQ=255) gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGa > 2:269873/1‑100 (MQ=255) gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGa > 1:1266412/1‑100 (MQ=255) aTCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGaa < 1:890780/100‑1 (MQ=255) aTCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGaa > 1:867786/1‑100 (MQ=255) aTCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGaa > 1:867527/1‑100 (MQ=255) gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTg > 1:1738019/1‑100 (MQ=255) cTTTTTCCCTTCAGGCATTTTTTTTTTTTTTTTTCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGATAAAAAA‑TGAAACGGTGGGAAGt > 2:1300693/1‑98 (MQ=255) tttttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑AAAAA‑TGAAACGGTGGGAAGTGGc < 1:1768745/99‑1 (MQ=255) ttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCttt < 2:55631/100‑1 (MQ=255) ttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCttt > 2:1082062/1‑100 (MQ=255) ccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACt < 1:848527/100‑1 (MQ=255) cTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTg > 2:1560154/1‑100 (MQ=255) ttCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGATAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTgg < 1:1300700/100‑1 (MQ=255) cAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTg < 2:421228/100‑1 (MQ=255) ggCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGAt > 1:1940815/1‑100 (MQ=255) ggCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑AAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATg < 1:315265/100‑1 (MQ=255) cATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATgg < 2:910353/100‑1 (MQ=255) aTTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGt > 2:2005962/1‑100 (MQ=255) aTTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGt > 2:2027757/1‑100 (MQ=255) aTTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑AAAAACTGAAACGGTGGGAAGTGGCTTTACTGGTGATGGt > 2:728084/1‑100 (MQ=255) tttttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTa > 1:1876064/1‑100 (MQ=255) ttttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTaa > 2:897920/1‑100 (MQ=255) ttttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTaa < 1:1649405/100‑1 (MQ=255) tttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTg > 1:1724178/1‑100 (MQ=255) ttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg < 1:2042573/100‑1 (MQ=255) ttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg < 2:1648770/100‑1 (MQ=255) tAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGt > 2:434556/1‑100 (MQ=255) tAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGt > 1:661894/1‑100 (MQ=255) aTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGttt > 2:728133/1‑100 (MQ=255) aTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGttt > 2:1982907/1‑100 (MQ=255) tcaatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGc < 1:434557/100‑1 (MQ=255) tcaatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGc < 2:1538095/100‑1 (MQ=255) aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg < 1:1330665/100‑1 (MQ=255) aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg < 2:1947302/100‑1 (MQ=255) atcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGt > 1:1716166/1‑100 (MQ=255) caaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAt < 1:1042929/100‑1 (MQ=255) caaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAt > 2:212560/1‑100 (MQ=255) caaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAt < 1:1394514/100‑1 (MQ=255) aaaGGCAGTTAACAACAATAAAAGAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAAc < 2:13343/100‑1 (MQ=255) ggCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgt > 1:1173548/1‑100 (MQ=255) cttccgatctcaaAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAagg < 2:14558/90‑1 (MQ=255) tCCCAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAagga < 1:693396/100‑1 (MQ=255) cccAACAACAAAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggag > 2:848027/1‑100 (MQ=255) acaaAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCa > 2:438024/1‑100 (MQ=255) acaaAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCa > 1:1820726/1‑100 (MQ=255) acaaAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCa > 1:1281726/1‑100 (MQ=255) caaAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggagatcggaag > 1:14558/1‑92 (MQ=255) caaAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCAt < 2:986036/100‑1 (MQ=255) caaAATAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCAt < 1:547370/100‑1 (MQ=255) aaTAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGaa < 1:736098/100‑1 (MQ=255) tAAAGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAAc < 2:1851098/100‑1 (MQ=255) aaaGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACt > 1:1807999/1‑100 (MQ=255) aaGATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtt > 1:1456260/1‑100 (MQ=255) gATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt < 1:725445/100‑1 (MQ=255) gATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt < 1:269874/100‑1 (MQ=255) gATGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt > 1:1523728/1‑100 (MQ=255) tGGGAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTaa > 1:1461781/1‑100 (MQ=255) ggAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAAt > 1:1836944/1‑99 (MQ=255) gAGGTAAAAAA‑TGAATCGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCg < 1:2009191/100‑1 (MQ=255) gAGGTAAAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTaa < 2:872019/97‑1 (MQ=255) | AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑AAAAA‑TGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCG > NC_000913/2563414‑2563607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |