Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9780AC22.9% 270.0 / 84.1 125intergenic (‑188/+120)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base A (41/43);  new base C (24/1);  total (80/45)
Fisher's exact test for biased strand distribution p-value = 1.22e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

Only 100 of 128 total aligned reads displayed.

AAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAT  >  NC_000913/3705880‑3706077
                                                                                                  |                                                                                                            
aaGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTaa                                                                                                             >  2:185475/1‑100 (MQ=255)
  gTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACg                                                                                                           >  1:1531632/1‑100 (MQ=255)
    aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                                         <  1:1934732/100‑1 (MQ=255)
    aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                                         <  2:135796/100‑1 (MQ=255)
     ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                                        <  2:447341/100‑1 (MQ=255)
       gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                      >  2:513448/1‑100 (MQ=255)
        ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                     <  1:1175273/100‑1 (MQ=255)
         tgcCGGGTGGCGCCGCACGCAGAGGCACAGCGCCCCCGGGGATACAGATTGTTGACAAAACGCGCCTTGTTTTTTCCGGGTGCGGGGGTAACACCCTAt                                                                                                     >  2:812873/1‑99 (MQ=255)
          gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAtgc                                                                                                   >  2:1239799/1‑98 (MQ=255)
          gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                   >  1:1692397/1‑100 (MQ=255)
            cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                                 <  1:1042429/100‑1 (MQ=255)
             ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                                <  2:100884/100‑1 (MQ=255)
             ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                                <  1:1276452/100‑1 (MQ=255)
             ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCggn                                                                                                <  2:130339/100‑2 (MQ=37)
                tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                             <  1:754256/100‑1 (MQ=255)
                 ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                                            <  2:2017305/100‑1 (MQ=255)
                  gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                          >  1:369723/1‑100 (MQ=255)
                  gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGACCTACa                                                                                          <  1:1230610/100‑1 (MQ=255)
                  gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATACCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                          >  2:1076651/1‑100 (MQ=255)
                      tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                                      <  1:1538744/100‑1 (MQ=255)
                      tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                                      <  2:1529846/100‑1 (MQ=255)
                       gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                     >  2:917648/1‑100 (MQ=255)
                          cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                  <  2:1330249/100‑1 (MQ=255)
                           gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                 <  1:1948479/100‑1 (MQ=255)
                           gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                 <  2:75817/100‑1 (MQ=255)
                           gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                 <  2:647344/100‑1 (MQ=255)
                           gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                 <  2:918729/100‑1 (MQ=255)
                              gagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCaaa                                                                              >  1:1580336/1‑100 (MQ=255)
                                ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                            >  2:1518837/1‑100 (MQ=255)
                                  cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                          >  1:225286/1‑100 (MQ=255)
                                    cagtgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                        <  2:1629131/96‑1 (MQ=255)
                                    caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                        <  1:917652/100‑1 (MQ=255)
                                      gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                                      >  2:1997445/1‑100 (MQ=255)
                                      gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAACTCAATAc                                                                      <  1:1078162/100‑1 (MQ=255)
                                              gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGa                                                             >  1:1145712/1‑100 (MQ=255)
                                                  ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt‑‑‑t                                                    <  1:1976458/100‑1 (MQ=255)
                                                   taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt‑‑‑tt                                                   >  1:1305967/1‑100 (MQ=255)
                                                    aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTc                                                  >  2:1962867/1‑100 (MQ=255)
                                                     cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                               <  1:831111/100‑1 (MQ=255)
                                                      aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTa                                             <  1:1493074/100‑1 (MQ=255)
                                                       gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTa                                             <  1:1814764/100‑1 (MQ=255)
                                                           gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                         >  1:1016987/1‑100 (MQ=255)
                                                            ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCt                                        >  1:90518/1‑100 (MQ=255)
                                                              gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGa                                      <  1:513449/100‑1 (MQ=255)
                                                                cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATa                                    <  1:1997463/100‑1 (MQ=255)
                                                                cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATa                                    <  2:1145706/100‑1 (MQ=255)
                                                                     tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCgg                               >  2:1530745/1‑100 (MQ=255)
                                                                        gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgc                            >  2:1740492/1‑100 (MQ=255)
                                                                         gaatttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAgcgc                          >  1:446531/11‑100 (MQ=17)
                                                                         cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                           >  1:1234768/1‑100 (MQ=255)
                                                                          attttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAgcgc                          >  1:1189078/10‑100 (MQ=17)
                                                                           ttttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATA‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCa                         >  2:1660720/9‑100 (MQ=18)
                                                                            tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATA‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCAt                        >  2:1717047/8‑100 (MQ=18)
                                                                             ttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                      >  1:933144/7‑100 (MQ=18)
                                                                             ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATc                       >  2:863018/7‑100 (MQ=21)
                                                                             gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                       <  1:607170/100‑1 (MQ=255)
                                                                             gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                       <  1:115794/100‑1 (MQ=255)
                                                                              tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                      <  2:90517/100‑1 (MQ=255)
                                                                              tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                      >  2:1295220/1‑100 (MQ=255)
                                                                               ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg                     >  2:802348/1‑100 (MQ=255)
                                                                               ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg                     >  1:1382930/1‑100 (MQ=255)
                                                                               gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                     >  1:1353622/5‑100 (MQ=17)
                                                                               gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                     >  2:724531/5‑100 (MQ=17)
                                                                                taggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                    <  2:1353613/97‑1 (MQ=17)
                                                                                taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAgg                    >  2:779351/4‑100 (MQ=18)
                                                                                taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATA‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                    >  1:482653/4‑100 (MQ=18)
                                                                                 aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                   >  1:826989/3‑100 (MQ=17)
                                                                                 aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTGTGCAAATTCAATATATTGCAGGAAA‑‑‑CAC‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGc                   >  2:170194/3‑100 (MQ=18)
                                                                                 aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                   >  2:529570/1‑100 (MQ=255)
                                                                                  tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                  >  2:1504659/1‑100 (MQ=255)
                                                                                  ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAACAGTGCAAATTCAATATATTGCAATCTT‑‑‑CTG‑‑GTAGGCCTGATAAGCATCGCGCATCAGGCa                  >  1:636226/2‑100 (MQ=18)
                                                                                  ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                  >  2:162785/2‑100 (MQ=17)
                                                                                  ggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                  >  2:576395/2‑100 (MQ=18)
                                                                                   gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                 >  1:1175680/1‑100 (MQ=255)
                                                                                   gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                 >  1:718180/1‑100 (MQ=255)
                                                                                   gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                 >  1:1238606/1‑99 (MQ=21)
                                                                                    ccggataaggcgTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATA‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                >  2:1724019/9‑98 (MQ=18)
                                                                                    ccggataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttt               >  1:96428/9‑100 (MQ=21)
                                                                                    ccGGATGCGGCGTTAACGCCTTATCCAGCCTACAACATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt                >  2:1408652/1‑100 (MQ=255)
                                                                                     cggataaggcgTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGATC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagtt               >  2:1176964/8‑97 (MQ=21)
                                                                                     cggataaggcgTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGATC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtt               >  1:1643817/8‑100 (MQ=18)
                                                                                      ngataaggcgTTCACGCCGCATCCGGCCTACAAAAATATGCAAATTCAATAGATTGCAGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt              >  1:1439136/7‑100 (MQ=17)
                                                                                      ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt              >  1:393829/7‑100 (MQ=17)
                                                                                      ggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaattc              >  1:1749440/7‑96 (MQ=21)
                                                                                      ggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaattc              >  1:1798937/7‑96 (MQ=21)
                                                                                      ggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt              >  1:507001/7‑100 (MQ=18)
                                                                                      ggataaggcgTTCACGCCGCATCCGGCCTACAAAACTGTGCAAATTCAATATATTGCAGGAAA‑‑‑CAC‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGCAgttt              >  2:866043/7‑100 (MQ=18)
                                                                                      ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt              <  1:1862894/100‑1 (MQ=255)
                                                                                       gataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtttt             >  1:806205/6‑100 (MQ=17)
                                                                                       gataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtttt             >  1:1526459/6‑100 (MQ=18)
                                                                                       gataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAAC‑‑‑GAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaactac             >  1:1824891/6‑95 (MQ=25)
                                                                                       gATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAttttt             <  1:1013930/100‑1 (MQ=255)
                                                                                         taaggcgTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCAatttgg            >  2:1131838/4‑98 (MQ=21)
                                                                                         tGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc           >  2:1134089/1‑100 (MQ=255)
                                                                                           cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct         >  2:440645/1‑100 (MQ=255)
                                                                                           cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct         >  2:422702/1‑100 (MQ=255)
                                                                                            ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt        >  1:1599143/1‑100 (MQ=255)
                                                                                                ttAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcc    <  1:372874/100‑1 (MQ=255)
                                                                                                  aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt  >  1:1571042/1‑100 (MQ=255)
                                                                                                  aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt  >  2:1014562/1‑100 (MQ=255)
                                                                                                  |                                                                                                            
AAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAT  >  NC_000913/3705880‑3706077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: