| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,627,706 | T→G | F131C (TTT→TGT) | nadR → | trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,627,706 | 0 | T | G | 97.3% | 257.0 / ‑0.8 | 73 | F131C (TTT→TGT) | nadR | trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (36/35); total (37/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
GGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAA > NC_000913/4627609‑4627802 | ggTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTa < 2:735462/100‑1 (MQ=255) ttGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAata > 1:278492/1‑100 (MQ=255) ttGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAata > 1:1304393/1‑100 (MQ=255) tGACGATACCCGAGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAatat > 1:1344961/1‑100 (MQ=255) aCGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCa > 2:833159/1‑100 (MQ=255) aTACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGaa < 1:784657/100‑1 (MQ=255) aCCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGaaaa > 1:615625/1‑100 (MQ=255) cccGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGaaaaa < 1:196575/100‑1 (MQ=255) cGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCAGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAata < 2:1259810/100‑1 (MQ=255) gACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTc < 1:769902/100‑1 (MQ=255) aCCGCGCGTTGTTCGAAGACGGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAAAATAAGAAAAATATTc < 2:596595/99‑1 (MQ=255) aCCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCg > 1:1414172/1‑100 (MQ=255) aCCGCGCGTTATTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTCATCGCAAACTTGTAAATATCAGAAAAATATTCg < 2:1344952/100‑1 (MQ=255) ncgcgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGc > 2:1876867/2‑100 (MQ=255) ccGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGc < 2:1987514/100‑1 (MQ=255) ccGCGCGTTGTTCAAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGc > 2:1265900/1‑100 (MQ=255) cgcgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCa > 1:328553/1‑100 (MQ=255) cgcgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCa < 1:1579187/100‑1 (MQ=255) gcgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCAt < 1:1517132/100‑1 (MQ=255) cgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCAtt > 1:1712461/1‑100 (MQ=255) gcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTc < 2:977533/100‑1 (MQ=255) tgttCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGc > 2:1972106/1‑100 (MQ=255) tgttCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGc > 1:897031/1‑100 (MQ=255) gttCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAGAATATTCGCATTCATGCt > 1:1193392/1‑100 (MQ=255) gttCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCt > 2:255010/1‑100 (MQ=255) cGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTc < 1:676827/100‑1 (MQ=255) gACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACg < 2:686729/100‑1 (MQ=255) cAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGaa < 1:1645368/100‑1 (MQ=255) cAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGaa > 2:1851209/1‑100 (MQ=255) aGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAag < 1:1576799/100‑1 (MQ=255) gCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAgg < 1:120363/100‑1 (MQ=255) ccATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATGTCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAggg > 1:1886858/1‑100 (MQ=255) ccATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAggg > 2:986811/1‑100 (MQ=255) cATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGc > 1:1989997/1‑100 (MQ=255) aTGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTGGTAAATGTCAGGAAAATATTCGCATTCATGCTTTCAACGAAGATGGCa > 1:1116610/1‑100 (MQ=255) tGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCAt < 2:328552/100‑1 (MQ=255) tCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATgg < 2:695954/100‑1 (MQ=255) tCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATgg < 2:615623/100‑1 (MQ=255) caacgcagcCGACTGTGCCGAATCGTCTGAGTTGTTTATTTTAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATg < 2:307711/94‑1 (MQ=255) gcagcaGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAg < 1:1172829/100‑1 (MQ=255) gcagcaGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAg > 2:1172823/1‑100 (MQ=255) gcagcaGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAg > 2:1775700/1‑100 (MQ=255) cagcagCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGc < 2:226754/100‑1 (MQ=255) gcagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCg < 2:1304385/100‑1 (MQ=255) gcagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCg < 1:1972124/100‑1 (MQ=255) gcagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCg < 2:278491/100‑1 (MQ=255) agcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTa > 2:184860/1‑100 (MQ=255) gcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGCGGGCCTGGAGCCGt > 1:1111896/1‑98 (MQ=255) gcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAt > 2:79977/1‑100 (MQ=255) gACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCg < 2:1290473/100‑1 (MQ=255) gACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCg > 1:34017/1‑100 (MQ=255) ccGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCa > 1:1311057/1‑100 (MQ=255) gCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGc > 2:589606/1‑100 (MQ=255) ccGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCt > 2:1878981/1‑100 (MQ=255) cGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTg > 2:725441/1‑100 (MQ=255) ggATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTgg < 2:1111891/100‑1 (MQ=255) gATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTggn < 1:1876884/100‑2 (MQ=255) aTCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGa > 2:2028302/1‑100 (MQ=255) tCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtgtg < 1:1265906/100‑1 (MQ=255) tGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtgtgtg < 1:1142077/100‑1 (MQ=255) cGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGa > 2:1143512/1‑100 (MQ=255) cGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGa > 2:103591/1‑100 (MQ=255) gTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAg < 2:421453/100‑1 (MQ=255) tATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACgg > 1:1639133/1‑100 (MQ=255) tATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACgg < 2:897028/100‑1 (MQ=255) ttGCAAACTTGTAAATATCAGAAAAATATTCGCATTCACGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCa < 2:1116605/100‑1 (MQ=255) tGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCAt < 1:307712/100‑1 (MQ=255) tGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCAt > 1:125971/1‑100 (MQ=255) gCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATc > 1:1770642/1‑100 (MQ=255) cAAACTTGTAAATATCAGAACAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCa > 2:1246224/1‑100 (MQ=255) cAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCa < 1:1588025/100‑1 (MQ=255) aCTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCaaaa < 2:195852/100‑1 (MQ=255) cTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCaaaaa > 2:1002903/1‑100 (MQ=255) | GGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAA > NC_000913/4627609‑4627802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |