Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 756,886 | T→A | L327Q (CTG→CAG) | sdhA → | succinate dehydrogenase, flavoprotein subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 756,886 | 0 | T | A | 98.7% | 266.9 / NA | 75 | L327Q (CTG→CAG) | sdhA | succinate dehydrogenase, flavoprotein subunit |
Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (39/35); total (39/36) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGG > NC_000913/756791‑756982 | cGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCg > 2:811230/1‑100 (MQ=255) gAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCgg > 1:1247772/1‑100 (MQ=255) gAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCgg > 1:908203/1‑100 (MQ=255) gAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGAGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCgg > 2:1095380/1‑100 (MQ=255) aaaTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCggg < 2:1554284/100‑1 (MQ=255) ccGTGAAGGTCGCGGCTGTGATAGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATc > 2:1187591/1‑100 (MQ=255) cGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATcc > 2:1711899/1‑100 (MQ=255) gTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCt > 2:353711/1‑100 (MQ=255) tGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTg < 2:912121/100‑1 (MQ=255) ngAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTg > 2:474022/2‑100 (MQ=255) gAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTgg < 1:1936968/100‑1 (MQ=255) aGGTCGCTGCTGTGATGGTCCGTGGGGGCCACATGCGAAACTGAATCTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAg < 2:2036428/100‑1 (MQ=255) aGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCCGGTATCCTGGAg < 2:24117/100‑1 (MQ=255) cgGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTcc < 1:707403/100‑1 (MQ=255) gCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCg < 1:649108/100‑1 (MQ=255) cTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGt < 2:896811/100‑1 (MQ=255) tgtgATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTa > 2:1573172/1‑100 (MQ=255) agctattgTCCGTGTGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTa < 1:1908109/93‑1 (MQ=255) tgATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTAcc < 2:914372/100‑1 (MQ=255) gATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCt > 1:1041900/1‑100 (MQ=255) aTGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCtt < 1:1963420/100‑1 (MQ=255) aTGGTACGTGGGGGCCACACGCGAAACTGAAACTAGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCTGGTATCCTGGAGCTTTCCCgcaagat > 2:1908091/1‑94 (MQ=255) tGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTc > 1:1523071/1‑100 (MQ=255) tGGTCCGTGGCGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGCAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTcc < 2:1820925/91‑1 (MQ=255) ggTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCg < 2:1523062/100‑1 (MQ=255) ccGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTc < 2:314468/100‑1 (MQ=255) gggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGt > 1:1813746/1‑100 (MQ=255) gggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGt > 2:443212/1‑100 (MQ=255) ggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTc < 2:1490186/100‑1 (MQ=255) gCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAt < 2:1555533/100‑1 (MQ=255) gCCACACGCGAAAATGAAACTCGATCACCTGGGTAAAGTAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAt > 2:1261768/1‑100 (MQ=255) ccACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATc > 1:844416/1‑100 (MQ=255) cacaCGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATcc > 2:1009467/1‑100 (MQ=255) acacGCGAAACTGAATCTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg < 2:677790/100‑1 (MQ=255) acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg > 2:955555/1‑100 (MQ=255) acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg > 1:285123/1‑100 (MQ=255) ccgatctactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGt < 2:385355/93‑1 (MQ=255) acGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGt < 1:443213/100‑1 (MQ=255) gcgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTgn < 2:1499026/100‑2 (MQ=255) cgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaa < 1:1261774/100‑1 (MQ=255) gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaaa < 1:1294161/100‑1 (MQ=255) aaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAag < 2:1166403/100‑1 (MQ=255) aaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAag < 2:1748760/100‑1 (MQ=255) aaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAag > 2:546910/1‑100 (MQ=255) actgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGtagatcgg > 1:385356/1‑93 (MQ=255) ctgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGc > 2:286333/1‑100 (MQ=255) ctgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGc > 1:1474499/1‑100 (MQ=255) ctCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTATCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTc > 2:1305691/1‑100 (MQ=255) cGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCg > 2:1858238/1‑100 (MQ=255) cGATCACCTGGGCAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCg > 1:1343032/1‑100 (MQ=255) aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGt < 1:2040651/100‑1 (MQ=255) aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGt < 1:955559/100‑1 (MQ=255) aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGt < 1:795613/100‑1 (MQ=255) cACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTa > 2:1402354/1‑100 (MQ=255) cTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATcc < 1:1155893/100‑1 (MQ=255) cTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATcc < 2:1544815/100‑1 (MQ=255) tGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATccc > 1:721825/1‑100 (MQ=255) tGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATccc > 1:723506/1‑100 (MQ=255) tAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc < 1:1858255/100‑1 (MQ=255) aaaGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCt > 1:1640729/1‑100 (MQ=255) aagaagTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTg > 1:820735/1‑100 (MQ=255) gaagTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTc > 1:1835639/1‑100 (MQ=255) agTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAc < 2:1474490/100‑1 (MQ=255) gTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACt < 2:315822/100‑1 (MQ=255) ctcGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACa > 2:334963/1‑100 (MQ=255) cGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatg < 1:749602/100‑1 (MQ=255) cGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatg > 2:1484155/1‑100 (MQ=255) cGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatg < 2:1608771/100‑1 (MQ=255) cGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACATCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatg > 2:940074/1‑100 (MQ=255) gAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatga > 2:1460664/1‑100 (MQ=255) ccGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGc > 2:751539/1‑100 (MQ=255) ccGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGc < 2:844413/100‑1 (MQ=255) ccGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGc > 1:1549822/1‑100 (MQ=255) ccGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGc > 1:1214673/1‑100 (MQ=255) gTCTGCCGGTTATCCTGGAGCTTTCCCTTACCTTCGCTCACGTCGACCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCgg < 2:1152897/100‑1 (MQ=255) | CGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGG > NC_000913/756791‑756982 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |