Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 756,886 T→A L327Q (CTG→CAG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,8860TA98.7% 266.9 / NA 75L327Q (CTG→CAG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (39/35);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGG  >  NC_000913/756791‑756982
                                                                                               |                                                                                                
cGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCg                                                                                              >  2:811230/1‑100 (MQ=255)
 gAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCgg                                                                                             >  1:1247772/1‑100 (MQ=255)
 gAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCgg                                                                                             >  1:908203/1‑100 (MQ=255)
 gAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGAGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCgg                                                                                             >  2:1095380/1‑100 (MQ=255)
  aaaTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCggg                                                                                            <  2:1554284/100‑1 (MQ=255)
      ccGTGAAGGTCGCGGCTGTGATAGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATc                                                                                        >  2:1187591/1‑100 (MQ=255)
       cGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATcc                                                                                       >  2:1711899/1‑100 (MQ=255)
        gTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCt                                                                                      >  2:353711/1‑100 (MQ=255)
         tGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTg                                                                                     <  2:912121/100‑1 (MQ=255)
         ngAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTg                                                                                     >  2:474022/2‑100 (MQ=255)
          gAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTgg                                                                                    <  1:1936968/100‑1 (MQ=255)
            aGGTCGCTGCTGTGATGGTCCGTGGGGGCCACATGCGAAACTGAATCTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAg                                                                                  <  2:2036428/100‑1 (MQ=255)
            aGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCCGGTATCCTGGAg                                                                                  <  2:24117/100‑1 (MQ=255)
                  cgGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTcc                                                                            <  1:707403/100‑1 (MQ=255)
                    gCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCg                                                                          <  1:649108/100‑1 (MQ=255)
                     cTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGt                                                                         <  2:896811/100‑1 (MQ=255)
                      tgtgATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTa                                                                        >  2:1573172/1‑100 (MQ=255)
                      agctattgTCCGTGTGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTa                                                                        <  1:1908109/93‑1 (MQ=255)
                        tgATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTAcc                                                                      <  2:914372/100‑1 (MQ=255)
                         gATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCt                                                                     >  1:1041900/1‑100 (MQ=255)
                          aTGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCtt                                                                    <  1:1963420/100‑1 (MQ=255)
                          aTGGTACGTGGGGGCCACACGCGAAACTGAAACTAGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCTGGTATCCTGGAGCTTTCCCgcaagat                                                                    >  2:1908091/1‑94 (MQ=255)
                           tGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTc                                                                   >  1:1523071/1‑100 (MQ=255)
                           tGGTCCGTGGCGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGCAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTcc                                                                            <  2:1820925/91‑1 (MQ=255)
                            ggTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCg                                                                  <  2:1523062/100‑1 (MQ=255)
                               ccGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTc                                                               <  2:314468/100‑1 (MQ=255)
                                   gggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGt                                                           >  1:1813746/1‑100 (MQ=255)
                                   gggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGt                                                           >  2:443212/1‑100 (MQ=255)
                                    ggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTc                                                          <  2:1490186/100‑1 (MQ=255)
                                       gCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAt                                                       <  2:1555533/100‑1 (MQ=255)
                                       gCCACACGCGAAAATGAAACTCGATCACCTGGGTAAAGTAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAt                                                       >  2:1261768/1‑100 (MQ=255)
                                        ccACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATc                                                      >  1:844416/1‑100 (MQ=255)
                                         cacaCGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATcc                                                     >  2:1009467/1‑100 (MQ=255)
                                          acacGCGAAACTGAATCTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg                                                    <  2:677790/100‑1 (MQ=255)
                                          acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg                                                    >  2:955555/1‑100 (MQ=255)
                                          acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg                                                    >  1:285123/1‑100 (MQ=255)
                                            ccgatctactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGt                                                  <  2:385355/93‑1 (MQ=255)
                                            acGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGt                                                  <  1:443213/100‑1 (MQ=255)
                                              gcgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTgn                                                <  2:1499026/100‑2 (MQ=255)
                                               cgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaa                                               <  1:1261774/100‑1 (MQ=255)
                                                gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaaa                                              <  1:1294161/100‑1 (MQ=255)
                                                 aaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAag                                             <  2:1166403/100‑1 (MQ=255)
                                                 aaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAag                                             <  2:1748760/100‑1 (MQ=255)
                                                 aaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAag                                             >  2:546910/1‑100 (MQ=255)
                                                   actgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGtagatcgg                                           >  1:385356/1‑93 (MQ=255)
                                                    ctgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGc                                          >  2:286333/1‑100 (MQ=255)
                                                    ctgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGc                                          >  1:1474499/1‑100 (MQ=255)
                                                          ctCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTATCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTc                                    >  2:1305691/1‑100 (MQ=255)
                                                            cGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCg                                  >  2:1858238/1‑100 (MQ=255)
                                                            cGATCACCTGGGCAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCg                                  >  1:1343032/1‑100 (MQ=255)
                                                              aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGt                                <  1:2040651/100‑1 (MQ=255)
                                                              aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGt                                <  1:955559/100‑1 (MQ=255)
                                                              aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGt                                <  1:795613/100‑1 (MQ=255)
                                                                cACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTa                              >  2:1402354/1‑100 (MQ=255)
                                                                   cTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATcc                           <  1:1155893/100‑1 (MQ=255)
                                                                   cTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATcc                           <  2:1544815/100‑1 (MQ=255)
                                                                    tGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATccc                          >  1:721825/1‑100 (MQ=255)
                                                                    tGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATccc                          >  1:723506/1‑100 (MQ=255)
                                                                        tAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                      <  1:1858255/100‑1 (MQ=255)
                                                                         aaaGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCt                     >  1:1640729/1‑100 (MQ=255)
                                                                          aagaagTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTg                    >  1:820735/1‑100 (MQ=255)
                                                                            gaagTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTc                  >  1:1835639/1‑100 (MQ=255)
                                                                              agTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAc                <  2:1474490/100‑1 (MQ=255)
                                                                               gTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACt               <  2:315822/100‑1 (MQ=255)
                                                                                  ctcGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACa            >  2:334963/1‑100 (MQ=255)
                                                                                    cGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatg          <  1:749602/100‑1 (MQ=255)
                                                                                    cGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatg          >  2:1484155/1‑100 (MQ=255)
                                                                                    cGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatg          <  2:1608771/100‑1 (MQ=255)
                                                                                    cGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACATCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatg          >  2:940074/1‑100 (MQ=255)
                                                                                     gAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatga         >  2:1460664/1‑100 (MQ=255)
                                                                                          ccGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGc    >  2:751539/1‑100 (MQ=255)
                                                                                          ccGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGc    <  2:844413/100‑1 (MQ=255)
                                                                                          ccGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGc    >  1:1549822/1‑100 (MQ=255)
                                                                                          ccGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGc    >  1:1214673/1‑100 (MQ=255)
                                                                                            gTCTGCCGGTTATCCTGGAGCTTTCCCTTACCTTCGCTCACGTCGACCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCgg  <  2:1152897/100‑1 (MQ=255)
                                                                                               |                                                                                                
CGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGG  >  NC_000913/756791‑756982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: