Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 882,856 | C→A | R178L (CGT→CTT) | ybjG ← | undecaprenyl pyrophosphate phosphatase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 882,856 | 0 | C | A | 98.4% | 224.2 / ‑3.4 | 63 | R178L (CGT→CTT) | ybjG | undecaprenyl pyrophosphate phosphatase |
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (27/35); total (27/36) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 |
AGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGG > NC_000913/882761‑882950 | aGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTg < 1:146441/100‑1 (MQ=255) gCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGa < 2:784336/100‑1 (MQ=255) gCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGa < 1:1901547/100‑1 (MQ=255) ccGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGAt < 2:1073314/100‑1 (MQ=255) ccGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGAt < 1:1265553/100‑1 (MQ=255) ccGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGAt > 1:1849534/1‑100 (MQ=255) ccGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGAt < 1:1732912/100‑1 (MQ=255) cGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGAtn < 2:149330/100‑2 (MQ=255) gCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATag < 2:1177846/100‑1 (MQ=255) aaTACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGtt < 2:369984/100‑1 (MQ=255) aaTACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGtn < 1:880805/100‑2 (MQ=255) ctacACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATg > 2:1653573/1‑100 (MQ=255) acacTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGcc > 2:116339/1‑100 (MQ=255) acacTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGcc < 1:1231865/100‑1 (MQ=255) cacTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGccc > 2:481798/1‑100 (MQ=255) tttttAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCAt > 1:1254801/1‑100 (MQ=255) cAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGc > 2:773721/1‑100 (MQ=255) cAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGc < 2:1849518/100‑1 (MQ=255) agagATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGcc > 1:309638/1‑100 (MQ=255) agagATCAGTCACGCACCCAGCCTCTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGcc < 1:1541571/100‑1 (MQ=255) gagaTCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCa < 2:1306052/100‑1 (MQ=255) gagaTCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCa > 2:887569/1‑100 (MQ=255) gagaTCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCa < 1:1906749/100‑1 (MQ=255) agaTCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAg < 1:428659/100‑1 (MQ=255) gaTCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGa > 2:1383625/1‑100 (MQ=255) aTCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGAt > 1:1235781/1‑100 (MQ=255) aTCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGAt < 1:481799/100‑1 (MQ=255) tCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATa < 1:1901703/100‑1 (MQ=255) tCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCt < 1:1292261/100‑1 (MQ=255) cACGCACCCAGCCTTTGTGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTg > 2:1719017/1‑100 (MQ=255) cACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTg < 2:1051558/100‑1 (MQ=255) cACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCa > 1:995209/1‑100 (MQ=255) cccAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACt > 1:1882143/1‑100 (MQ=255) ccAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTa < 1:1634255/100‑1 (MQ=255) cAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTaa > 2:1503553/1‑100 (MQ=255) ccTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGc < 2:971926/100‑1 (MQ=255) ttGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGc < 1:1451120/100‑1 (MQ=255) ttGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGc < 2:1892400/100‑1 (MQ=255) tGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGcc < 1:1503562/100‑1 (MQ=255) gATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATc > 2:616428/1‑100 (MQ=255) gATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATc < 2:1235775/100‑1 (MQ=255) gATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATc < 2:6687/100‑1 (MQ=255) cGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCAt < 2:1894598/99‑1 (MQ=255) cGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATa < 1:1841745/100‑1 (MQ=255) aaTGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTg > 2:1620292/1‑100 (MQ=255) aaTGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTg > 2:177124/1‑100 (MQ=255) tGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGcc < 2:1127961/100‑1 (MQ=255) gCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCa > 2:162987/1‑100 (MQ=255) cAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAg > 2:773339/1‑100 (MQ=255) cAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAg < 2:816039/100‑1 (MQ=255) aaaaCAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGc > 1:399605/1‑100 (MQ=255) aaaCAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCa > 2:815786/1‑100 (MQ=255) aCAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAAt < 1:1620176/100‑1 (MQ=255) cAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGTCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATc < 1:1520279/100‑1 (MQ=255) cgcgATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCAcc > 2:1059304/1‑100 (MQ=255) cgcgATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCAcc < 1:2040859/100‑1 (MQ=255) cgATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGa > 2:2028565/1‑100 (MQ=255) ccACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCata > 1:856544/1‑100 (MQ=255) ccACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCat > 2:1866796/1‑99 (MQ=255) aCGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATc < 2:995205/100‑1 (MQ=255) ttGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGc > 2:234420/1‑100 (MQ=255) ttGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGc < 1:670442/100‑1 (MQ=255) gCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCgg > 2:1761668/1‑100 (MQ=255) | AGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGG > NC_000913/882761‑882950 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |