| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,143,125 | C→T | D415N (GAC→AAC) | rne ← | endoribonuclease; RNA‑binding protein;RNA degradosome binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,143,125 | 0 | C | T | 98.2% | 190.4 / ‑5.0 | 56 | D415N (GAC→AAC) | rne | endoribonuclease; RNA‑binding protein;RNA degradosome binding protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (26/29); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGG > NC_000913/1143031‑1143219 | ggCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtacgca > 2:2013131/1‑100 (MQ=255)ggCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtacgca > 2:1180025/1‑100 (MQ=255) caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGt > 2:231668/1‑100 (MQ=255) aGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTg > 2:68998/1‑100 (MQ=255) ggAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGc > 1:140479/1‑100 (MQ=255) gAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGcc < 1:2004802/100‑1 (MQ=255) gAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGcc < 1:717256/100‑1 (MQ=255) tcttccgatctGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCa < 1:1016048/90‑1 (MQ=255) aCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAg < 1:429425/100‑1 (MQ=255) cAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGt < 1:231376/100‑1 (MQ=255) aTGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTAc < 1:1047006/100‑1 (MQ=255) aTGGCGTGAACTTCCCGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTAc < 1:2013149/100‑1 (MQ=255) tGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTAcc < 2:140478/100‑1 (MQ=255) cGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGa < 1:128382/100‑1 (MQ=255) cGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGa > 2:810069/1‑100 (MQ=255) gTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaa < 2:676264/100‑1 (MQ=255) gTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaa > 2:1149662/1‑100 (MQ=255) gTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaa > 1:1361805/1‑100 (MQ=255) tGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaac < 1:1308870/100‑1 (MQ=255) aCTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaacaac > 2:1560189/1‑100 (MQ=255) ttCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAAcgc > 2:1469134/1‑100 (MQ=255) tctcTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtg > 2:218286/1‑100 (MQ=255) tctcTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtg > 2:1975554/1‑100 (MQ=255) tctcTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtg > 2:1045260/1‑100 (MQ=255) ctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtgatg > 2:861307/1‑100 (MQ=255) tttCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtgatga > 2:326760/1‑100 (MQ=255) ttCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGAc < 1:164973/100‑1 (MQ=255) tCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACt > 1:1881490/1‑100 (MQ=255) cAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTCCGCACGGTGCCAGTACCAGAACAACGCAGACAAACGTGATGACTg < 1:1180031/100‑1 (MQ=255) cAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTg < 1:1149668/100‑1 (MQ=255) aGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTgg < 2:904555/100‑1 (MQ=255) aGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTgg > 1:862580/1‑100 (MQ=255) cttcttcttCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTc > 2:1727527/1‑100 (MQ=255) tcttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCAc < 2:643774/100‑1 (MQ=255) tcttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCAc < 1:1324253/100‑1 (MQ=255) cttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGCTGACTGCATTCAcc > 2:1385695/1‑100 (MQ=255) ttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCAccc < 2:1879947/100‑1 (MQ=255) ttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCAccc < 1:1681683/100‑1 (MQ=255) tcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCa > 1:1319835/1‑100 (MQ=255) tcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTg > 2:1865885/1‑100 (MQ=255) aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg < 2:733590/100‑1 (MQ=255) tCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATgg < 1:816814/100‑1 (MQ=255) cGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCa < 2:229256/100‑1 (MQ=255) cAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAgg > 2:502629/1‑100 (MQ=255) gAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGgcg < 2:1149261/100‑1 (MQ=255) gagagCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGa > 1:89150/1‑99 (MQ=255) gagagCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGAc > 2:99397/1‑100 (MQ=255) gagagCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGAc > 1:699002/1‑100 (MQ=255) gagCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACgg > 2:1351719/1‑100 (MQ=255) gCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGa < 2:1306681/100‑1 (MQ=255) ctaCAGCGATGCGTTGTTACGTACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGAc > 2:1322134/3‑100 (MQ=255) cGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACggggc > 1:1058499/1‑98 (MQ=255) cGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGAc < 2:512787/100‑1 (MQ=255) cGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGAc < 1:670157/100‑1 (MQ=255) cGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTcc < 1:810072/100‑1 (MQ=255) aTTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcag < 2:466864/100‑1 (MQ=255) aTTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcag > 1:266031/1‑100 (MQ=255) ttCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagc < 1:326761/100‑1 (MQ=255) gttgttACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAgg > 2:1593205/1‑100 (MQ=255) | GGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGG > NC_000913/1143031‑1143219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |