Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,460,438 T→C L462S (TTG→TCG)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,460,4380TC99.1% 427.0 / ‑4.9 116L462S (TTG→TCG) paaH3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (63/52);  total (63/53)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 119 total aligned reads displayed.

CTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGC  >  NC_000913/1460341‑1460534
                                                                                                 |                                                                                                
cTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTCTGGCGAAGAACGCTATCGCCCATGTTCATcgc                                                                                                <  2:890027/100‑1 (MQ=255)
cTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATcgc                                                                                                <  2:1148200/100‑1 (MQ=255)
   gCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATcgctgc                                                                                             <  2:1681154/100‑1 (MQ=255)
    ccTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTgcg                                                                                            >  1:261334/1‑100 (MQ=255)
    ccTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTgcg                                                                                            <  1:1292517/100‑1 (MQ=255)
    ccTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTgcg                                                                                            <  1:1796753/100‑1 (MQ=255)
     cTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTgcgc                                                                                           >  2:363503/1‑100 (MQ=255)
     cTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTgcgc                                                                                           >  2:172792/1‑100 (MQ=255)
      tGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGcc                                                                                          >  2:640450/1‑100 (MQ=255)
      tGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGcc                                                                                          <  1:1357908/100‑1 (MQ=255)
      tGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGcc                                                                                          <  1:602860/100‑1 (MQ=255)
       ggggAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCa                                                                                         >  1:431155/1‑100 (MQ=255)
         ggAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAAc                                                                                       >  2:443936/1‑100 (MQ=255)
           aGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACgg                                                                                     >  1:430342/1‑100 (MQ=255)
            gcgcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACggg                                                                                    >  2:313645/1‑100 (MQ=255)
             ngcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGgc                                                                                   >  1:272221/2‑100 (MQ=255)
             cgcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGgc                                                                                   <  1:343445/100‑1 (MQ=255)
              gcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACACCATCACTATGGCGAACAACGCAACCGCCCATGTTCATCGCTGCGCCAACGGgcg                                                                                  >  1:1401740/1‑100 (MQ=255)
               cAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGgcgc                                                                                 >  1:345604/1‑100 (MQ=255)
                    tggctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTg                                                                            <  2:1634882/100‑1 (MQ=255)
                      gctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTgg                                                                           <  1:1598896/99‑1 (MQ=255)
                       ctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGgag                                                                         <  2:292554/100‑1 (MQ=255)
                       ctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGgag                                                                         >  1:271280/1‑100 (MQ=255)
                         ggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGagag                                                                       <  2:1504661/100‑1 (MQ=255)
                         ggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGagag                                                                       >  1:420964/1‑100 (MQ=255)
                          gcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGc                                                                      <  1:41599/100‑1 (MQ=255)
                           cAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACGATGACGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCg                                                                     >  2:1152639/1‑100 (MQ=255)
                              cGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGtt                                                                  >  1:1499648/1‑100 (MQ=255)
                                aaTATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTAt                                                                <  1:1720115/100‑1 (MQ=255)
                                aaTATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTAt                                                                <  1:1107662/100‑1 (MQ=255)
                                 atatTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATg                                                               <  2:791157/100‑1 (MQ=255)
                                 atatTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATg                                                               >  2:95493/1‑100 (MQ=255)
                                   atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAg                                                             >  2:1316936/1‑100 (MQ=255)
                                   atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAg                                                             >  1:1267704/1‑100 (MQ=255)
                                    ttAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGt                                                            >  2:212511/1‑100 (MQ=255)
                                    ttAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGt                                                            >  1:1973994/1‑100 (MQ=255)
                                     tAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTc                                                           >  2:1787247/1‑100 (MQ=255)
                                      aaGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCa                                                          <  1:1484721/100‑1 (MQ=255)
                                      aaGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCa                                                          <  2:330548/100‑1 (MQ=255)
                                      aaGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCa                                                          >  2:1831215/1‑100 (MQ=255)
                                      aaGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCa                                                          >  2:1893143/1‑100 (MQ=255)
                                        ggCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCAtn                                                        <  2:1988541/100‑2 (MQ=255)
                                        ggCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCAt                                                         >  1:98556/1‑99 (MQ=255)
                                          cTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAg                                                      <  1:640452/100‑1 (MQ=255)
                                           tCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAgg                                                     <  1:163641/100‑1 (MQ=255)
                                             cTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAgatcggaagag                                                   >  2:37103/1‑90 (MQ=255)
                                             cTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGcc                                                   >  1:257736/1‑100 (MQ=255)
                                              ttGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCt                                                  >  2:670793/1‑100 (MQ=255)
                                               tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTg                                                 <  1:1947688/100‑1 (MQ=255)
                                               tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTg                                                 >  2:326101/1‑100 (MQ=255)
                                               tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTg                                                 <  2:1711373/100‑1 (MQ=255)
                                               tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTg                                                 >  2:1525651/1‑100 (MQ=255)
                                                gAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTgg                                                <  2:55577/100‑1 (MQ=255)
                                                   aaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCaa                                             >  1:480863/1‑100 (MQ=255)
                                                   aaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCaa                                             >  2:1926215/1‑100 (MQ=255)
                                                    aTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCaaa                                            >  1:400969/1‑100 (MQ=255)
                                                    aTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCaaa                                            <  2:261333/100‑1 (MQ=255)
                                                     tCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCaaaa                                           >  2:545566/1‑100 (MQ=255)
                                                      cTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAAt                                          <  1:1694350/100‑1 (MQ=255)
                                                        aCAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGc                                        <  1:24956/100‑1 (MQ=255)
                                                         cAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGcc                                       <  1:326102/100‑1 (MQ=255)
                                                          aGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGccc                                      <  1:1787262/100‑1 (MQ=255)
                                                          aGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGccc                                      <  2:1342208/100‑1 (MQ=255)
                                                           gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                     >  2:1613139/1‑100 (MQ=255)
                                                           gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                     >  1:959585/1‑100 (MQ=255)
                                                           gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                     <  1:648962/100‑1 (MQ=255)
                                                            cATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCAt                                    >  2:1905428/1‑100 (MQ=255)
                                                                 cTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAAt                               >  2:600104/1‑100 (MQ=255)
                                                                 cTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAAt                               >  1:659246/1‑100 (MQ=255)
                                                                   aTGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGt                             <  1:1992359/100‑1 (MQ=255)
                                                                    tGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTa                            <  2:1499639/100‑1 (MQ=255)
                                                                    tGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTa                            <  1:79256/100‑1 (MQ=255)
                                                                    tGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTa                            <  1:313646/100‑1 (MQ=255)
                                                                     ggCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTAt                           >  1:1985363/1‑100 (MQ=255)
                                                                      gCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATg                          <  1:1161654/100‑1 (MQ=255)
                                                                      gCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATg                          >  1:1958163/1‑100 (MQ=255)
                                                                      gCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATg                          <  1:968539/100‑1 (MQ=255)
                                                                        gaagaaCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATgag                        <  1:729738/100‑1 (MQ=255)
                                                                         aagaaCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATgaga                       <  2:1744597/100‑1 (MQ=255)
                                                                           gaaCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAAc                     >  2:508798/1‑100 (MQ=255)
                                                                           gaaCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAAc                     <  2:521271/100‑1 (MQ=255)
                                                                           gaaCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAAc                     >  2:752481/1‑100 (MQ=255)
                                                                           gaaCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAAc                     >  1:1855473/1‑100 (MQ=255)
                                                                            aaCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACg                    <  1:443937/100‑1 (MQ=255)
                                                                            aaCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACg                    <  1:1388631/100‑1 (MQ=255)
                                                                             aCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGa                   >  1:1758394/1‑100 (MQ=255)
                                                                               gCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATg                 <  1:545567/100‑1 (MQ=255)
                                                                                cTATCGCCCACGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGc                >  2:213375/1‑100 (MQ=255)
                                                                                 tATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATgn                <  2:272220/99‑2 (MQ=255)
                                                                                   tCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTg             <  1:1700970/100‑1 (MQ=255)
                                                                                    cGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgc            <  1:1205973/100‑1 (MQ=255)
                                                                                     gCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcg           >  2:599713/1‑100 (MQ=255)
                                                                                      cccATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcgc          >  1:1018093/1‑100 (MQ=255)
                                                                                       ccATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGcc         >  2:814536/1‑100 (MQ=255)
                                                                                       ccATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGcc         >  1:174574/1‑100 (MQ=255)
                                                                                          tGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCaaa      <  2:1758380/100‑1 (MQ=255)
                                                                                           gTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAg     >  2:885906/1‑100 (MQ=255)
                                                                                            ttCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgc    <  2:543147/100‑1 (MQ=255)
                                                                                             tCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgcg   >  1:1823770/1‑100 (MQ=255)
                                                                                              cATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgcgc  >  2:1293562/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGC  >  NC_000913/1460341‑1460534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: