Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,720,828	C→T	intergenic (‑4/‑197)	slyA ← / → ydhI	global transcriptional regulator/DUF1656 family putative inner membrane efflux pump associated protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,720,828	0	C	T	97.9%	335.5 / ‑2.3	96	intergenic (‑4/‑197)	slyA/ydhI	global transcriptional regulator/DUF1656 family putative inner membrane efflux pump associated protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (47/47);  total (48/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

TTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTT  >  NC_000913/1720731‑1720923                                                                                                  |                                                                                                tttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTAt                                                                                               <  1:279475/100‑1 (MQ=255)  ttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTAtc                                                                                              <  1:1350396/100‑1 (MQ=255)   tgtgTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTAtct                                                                                             <  1:192742/100‑1 (MQ=255)    gtgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTAtctc                                                                                            >  2:907086/1‑100 (MQ=255)    gtgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTAtctc                                                                                            >  2:282701/1‑100 (MQ=255)    gtgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTAtctc                                                                                            >  1:189098/1‑100 (MQ=255)     tgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTcc                                                                                           >  1:51499/1‑100 (MQ=255)     tgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTcc                                                                                           >  1:1668683/1‑100 (MQ=255)     tgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTcc                                                                                           <  1:1809274/100‑1 (MQ=255)      gtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCt                                                                                          >  1:1034869/1‑100 (MQ=255)      gtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCt                                                                                          >  2:1182756/1‑100 (MQ=255)         aaCTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTtat                                                                                       <  2:1984120/100‑1 (MQ=255)          aCTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTtata                                                                                      <  2:2006257/100‑1 (MQ=255)            tCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAAt                                                                                    <  2:1278025/100‑1 (MQ=255)                gCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGc                                                                                <  2:256418/100‑1 (MQ=255)                    tttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGc                                                                            >  2:1770819/1‑100 (MQ=255)                    tttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGc                                                                            <  1:1492880/100‑1 (MQ=255)                     ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCGGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCt                                                                           >  1:1779201/1‑100 (MQ=255)                      tCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTa                                                                          <  2:1668670/100‑1 (MQ=255)                        aGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAg                                                                        >  2:2000273/1‑100 (MQ=255)                         ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGa                                                                       >  2:72345/1‑100 (MQ=255)                         ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGa                                                                       >  2:1317837/1‑100 (MQ=255)                         ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGa                                                                       >  2:336286/1‑100 (MQ=255)                          gCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGat                                                                      <  1:1502459/100‑1 (MQ=255)                              tGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGAtatta                                                                  <  2:232304/100‑1 (MQ=255)                              tGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGAtatta                                                                  <  2:232497/100‑1 (MQ=255)                              tGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGAtatta                                                                  >  2:1747626/1‑100 (MQ=255)                               ggTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGAtattat                                                                 >  1:300843/1‑100 (MQ=255)                                gTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAACTAGCTTGCTAAGAtattacg                                                                <  2:582987/100‑3 (MQ=255)                                     tCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCt                                                           >  1:1027923/1‑100 (MQ=255)                                      cAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtt                                                          <  1:72345/100‑1 (MQ=255)                                      cAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtt                                                          >  2:274921/1‑100 (MQ=255)                                       agagCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCttt                                                         >  1:1764989/1‑100 (MQ=255)                                       agagCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCttt                                                         >  1:1387169/1‑100 (MQ=255)                                        gagCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtttt                                                        <  1:2033274/100‑1 (MQ=255)                                         agCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTa                                                       <  1:907090/100‑1 (MQ=255)                                          gCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg                                                      <  2:1240917/100‑1 (MQ=255)                                          gCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg                                                      <  1:1140081/100‑1 (MQ=255)                                          gCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg                                                      <  2:51499/100‑1 (MQ=255)                                            aCGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg                                                      <  2:758284/98‑1 (MQ=255)                                            aCGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGaa                                                    >  2:1305660/1‑100 (MQ=255)                                             cGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAt                                                   <  2:201698/100‑1 (MQ=255)                                             cGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAt                                                   >  2:377664/1‑100 (MQ=255)                                               ccATATGCGCACCAACTGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAg                                                 <  2:1199806/100‑1 (MQ=255)                                               ccATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAg                                                 >  2:1417707/1‑100 (MQ=255)                                               ccATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAg                                                 >  2:574170/1‑100 (MQ=255)                                                 atatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtg                                               <  1:498324/100‑1 (MQ=255)                                                   atGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGtgtg                                             >  1:1898083/1‑100 (MQ=255)                                                    tGCGCACCCACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgc                                            <  1:1452914/100‑1 (MQ=255)                                                    tGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgc                                            <  2:211645/100‑1 (MQ=255)                                                    tGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgc                                            <  1:24690/100‑1 (MQ=255)                                                    tGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgc                                            >  1:662441/1‑100 (MQ=255)                                                     gcgcACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgca                                           >  2:2043992/1‑100 (MQ=255)                                                     gcgcACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgca                                           >  2:445463/1‑100 (MQ=255)                                                     gcgcACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgca                                           <  1:1625285/100‑1 (MQ=255)                                                      ngcACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcag                                          >  2:1209790/2‑100 (MQ=255)                                                      cgcACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcag                                          >  1:1630235/1‑100 (MQ=255)                                                      cgcACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcag                                          <  1:336287/100‑1 (MQ=255)                                                      cgcACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcag                                          >  1:1535224/1‑100 (MQ=255)                                                       gcACCAACCGTGCCAGATCGGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagc                                         <  2:1779186/100‑1 (MQ=255)                                                       gcACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcag                                          <  2:1647054/99‑1 (MQ=255)                                                        cACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagca                                        >  1:1353574/1‑100 (MQ=255)                                                        cACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagca                                        >  1:1901754/1‑100 (MQ=255)                                                         aCCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCaa                                       >  1:64854/1‑100 (MQ=255)                                                            aaCCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTg                                    <  1:445464/100‑1 (MQ=255)                                                            aaCCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTg                                    <  2:189097/100‑1 (MQ=255)                                                             aCCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGt                                   >  1:444012/1‑100 (MQ=255)                                                               cGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTAt                                 >  2:1448434/1‑100 (MQ=255)                                                                 tGCCAGATTAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTg                               <  2:1898065/100‑1 (MQ=255)                                                                 tGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTg                               >  1:455244/1‑100 (MQ=255)                                                                  gCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGc                              <  1:1358537/100‑1 (MQ=255)                                                                   ccAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCt                             <  2:1480616/100‑1 (MQ=255)                                                                   ccAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCt                             <  1:270837/100‑1 (MQ=255)                                                                   ccAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGAGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCt                             >  2:1438353/1‑100 (MQ=255)                                                                    cAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTa                            >  1:1569379/1‑100 (MQ=255)                                                                    cAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTa                            <  1:262541/100‑1 (MQ=255)                                                                       aTCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTaaaa                         >  1:265077/1‑100 (MQ=255)                                                                       aTCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTaaaa                         <  1:18220/100‑1 (MQ=255)                                                                       aTCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTaaaa                         >  1:468506/1‑100 (MQ=255)                                                                       aTCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTaaaa                         >  2:1504586/1‑100 (MQ=255)                                                                         cAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACa                       <  1:1770833/100‑1 (MQ=255)                                                                          aGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACaa                      <  2:1366783/100‑1 (MQ=255)                                                                           gAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACaaa                     <  2:1357625/100‑1 (MQ=255)                                                                             aCCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATg                   <  2:150703/100‑1 (MQ=255)                                                                               cTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATg                   <  2:1027918/98‑1 (MQ=255)                                                                                 aGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTAtt               >  2:62717/1‑100 (MQ=255)                                                                                   tGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATtgc             <  1:1747640/100‑1 (MQ=255)                                                                                    ggCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATtgct            >  2:1555498/1‑100 (MQ=255)                                                                                      cGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGATTATTGTGCAGCAATTTTATTGCTAAAACAAATGTATttcttc          >  2:1971620/1‑98 (MQ=255)                                                                                      cGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATtgctgc          <  1:274922/100‑1 (MQ=255)                                                                                        aTTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCAt        <  2:1694347/100‑1 (MQ=255)                                                                                           ccAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTg     >  1:1660178/1‑100 (MQ=255)                                                                                           ccAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTg     >  1:1804247/1‑100 (MQ=255)                                                                                            cAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTgg    >  2:1964287/1‑100 (MQ=255)                                                                                             aaTTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGt   <  2:1034864/100‑1 (MQ=255)                                                                                              aTTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGtt  >  1:250628/1‑100 (MQ=255)                                                                                                  |                                                                                                TTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTT  >  NC_000913/1720731‑1720923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑