| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,827,031 | T→A | D410V (GAT→GTT) | astB ← | succinylarginine dihydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,827,031 | 0 | T | A | 96.4% | 184.6 / ‑2.5 | 56 | D410V (GAT→GTT) | astB | succinylarginine dihydrolase |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base A (31/23); total (33/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
CTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCC > NC_000913/1826934‑1827127 | ctcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACg < 2:654927/100‑1 (MQ=255) tcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACgg > 1:1055901/1‑100 (MQ=255) cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgc < 2:592528/100‑1 (MQ=255) gcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagn < 1:1777982/100‑2 (MQ=255) ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGt > 2:921390/1‑100 (MQ=255) ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGt > 2:1934465/1‑100 (MQ=255) cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAgg > 1:1652090/1‑100 (MQ=255) cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAgg < 1:1351397/100‑1 (MQ=255) aTAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGa > 2:644184/1‑100 (MQ=255) aaaCCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATc > 1:1964611/1‑100 (MQ=255) accgaaccgaGATTCAGTAATTGGCTCACTACATCCAGTGCTTCACGCCCTTCGCGCAGCACTTTCGGGTCGGCCAGCACGGCAGCAGTAAGGCCCTcgc > 2:1233495/1‑100 (MQ=255) ccgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcgcg < 1:1453748/100‑1 (MQ=255) aaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgta > 2:1793741/1‑100 (MQ=255) accgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtag < 2:1527662/100‑1 (MQ=255) ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGgtagt > 2:811222/1‑100 (MQ=255) ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagt < 1:686058/100‑1 (MQ=255) cgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagta < 2:735905/100‑1 (MQ=255) cgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagta < 1:502644/100‑1 (MQ=255) cAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATcc > 2:1426916/1‑100 (MQ=255) aGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCa < 2:10095/100‑1 (MQ=255) tAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAcc < 1:1803818/100‑1 (MQ=255) aTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCa > 1:1594366/1‑100 (MQ=255) ttGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAg < 1:509210/100‑1 (MQ=255) ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTc > 1:1880521/1‑100 (MQ=255) naaTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTg > 1:307360/2‑100 (MQ=255) cAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTg < 1:346482/100‑1 (MQ=255) cAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTg < 1:1767786/100‑1 (MQ=255) aaTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGa < 1:1225843/100‑1 (MQ=255) tACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgc < 1:1510475/100‑1 (MQ=255) aCATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcg > 1:846380/1‑100 (MQ=255) ntGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGACCCACCCAGTCATTGAGCGCATTaaa > 2:1418764/2‑100 (MQ=255) gTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCCGTCATTGAGCGCATTaaa > 1:1427002/1‑100 (MQ=255) gTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTaaa > 1:1515264/1‑100 (MQ=255) gCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACa > 2:623583/1‑100 (MQ=255) gCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACa > 1:1528077/1‑100 (MQ=255) cTTCCCGCCCTTCGCGCAGCAATTGCGCGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAg > 2:458247/1‑100 (MQ=255) tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg > 2:1446189/1‑100 (MQ=255) cccGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGt > 2:483292/1‑100 (MQ=255) cGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTAt > 1:332185/1‑100 (MQ=255) tccgatctcgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGGAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATc < 1:327503/92‑1 (MQ=255) cccTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCg < 2:714687/100‑1 (MQ=255) tCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtca < 1:1934483/100‑1 (MQ=255) cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATcagatcgga > 2:327502/1‑92 (MQ=255) gcaATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAc < 1:921394/100‑1 (MQ=255) aTTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgc > 1:860885/1‑100 (MQ=255) ttGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgcc < 1:232127/100‑1 (MQ=255) ttGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgcc < 2:745546/100‑1 (MQ=255) tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg > 1:975893/1‑100 (MQ=255) gggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGAt > 2:1936321/1‑100 (MQ=255) ggCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAcc > 2:1871180/1‑100 (MQ=255) ggCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAcc < 1:623585/100‑1 (MQ=255) gCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCg > 2:1741622/1‑100 (MQ=255) gCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCg > 1:992896/1‑100 (MQ=255) gCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCg > 1:970304/1‑100 (MQ=255) ccAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGc > 1:1212060/1‑100 (MQ=255) aGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGccc > 1:1432178/1‑100 (MQ=255) | CTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCC > NC_000913/1826934‑1827127 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |