| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,281,279 | A→T | A216A (GCA→GCT) | yejH → | putative ATP‑dependent DNA or RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,281,279 | 0 | A | T | 98.4% | 214.3 / ‑3.7 | 62 | A216A (GCA→GCT) | yejH | putative ATP‑dependent DNA or RNA helicase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (40/21); total (41/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
GCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGT > NC_000913/2281183‑2281373 | gCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCag < 1:434518/100‑1 (MQ=255)gCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCag > 1:589037/1‑100 (MQ=255) cTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCaga < 1:1585577/100‑1 (MQ=255) gCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAc > 1:499113/1‑100 (MQ=255) cTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑g > 1:101866/1‑100 (MQ=255) tGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑gg < 1:192192/100‑1 (MQ=255) ccgatcttatGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑gg < 2:1730590/93‑1 (MQ=255) ttATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTg < 2:202834/100‑1 (MQ=255) tataTGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGt > 1:817774/1‑100 (MQ=255) tatGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑ggagatcgg > 1:1730603/1‑93 (MQ=255) tatGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTc < 1:1499566/100‑1 (MQ=255) gATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGc < 2:541577/100‑1 (MQ=255) aaaCACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAg > 2:1394717/1‑100 (MQ=255) cacGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCg > 1:1745269/1‑100 (MQ=255) cacGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCg > 2:29286/1‑100 (MQ=255) cTATCTGACGCCGCCAGAACGACTGTATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGAtct < 2:1307943/99‑1 (MQ=255) cTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGAtctc > 1:119559/1‑100 (MQ=255) tGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCg > 1:1443226/1‑100 (MQ=255) tGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCg < 2:499112/100‑1 (MQ=255) gccgccAGAACGGCTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGa < 2:817771/99‑1 (MQ=255) ccgccAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAg > 1:1315051/1‑100 (MQ=255) cAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaa > 2:1017472/1‑100 (MQ=255) aGAACGCCTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaaa < 1:1322446/100‑1 (MQ=255) aGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaaa > 1:1349640/1‑100 (MQ=255) cGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaac > 2:615801/1‑100 (MQ=255) ggATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaacaa > 1:163052/1‑100 (MQ=255) ggATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaacaa > 2:1346752/1‑100 (MQ=255) ggATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaaca > 1:825598/1‑99 (MQ=255) atatGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACg < 1:1909499/100‑1 (MQ=255) tatGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGt < 1:1346761/100‑1 (MQ=255) tatGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGt > 1:1683151/1‑100 (MQ=255) atGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTa < 2:1745255/100‑1 (MQ=255) tGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTAt > 1:484412/1‑100 (MQ=255) tGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTAt > 2:588835/1‑100 (MQ=255) gCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTAtt > 1:748917/1‑100 (MQ=255) gCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTAtt > 1:2043321/1‑100 (MQ=255) ccAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTa > 1:1145298/1‑100 (MQ=255) cAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTAc > 2:145959/1‑100 (MQ=255) gtagtGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGTTCTCAACCGTGAGCTGAAAAAACAACAACGTATTAccc > 2:454853/1‑100 (MQ=255) tGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGca > 2:1956879/1‑100 (MQ=255) gCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac > 1:1662590/1‑100 (MQ=255) gCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac > 1:448012/1‑100 (MQ=255) gCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac > 2:440929/1‑100 (MQ=255) gCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac > 2:410868/1‑100 (MQ=255) cAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcaca > 2:1951042/1‑100 (MQ=255) aaTACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCAcat > 2:549568/1‑100 (MQ=255) aaTACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCAcat < 1:1280798/100‑1 (MQ=255) tACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCAcatca > 1:1234244/1‑100 (MQ=255) tACGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCAcatca < 1:925589/100‑1 (MQ=255) cGATTTCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACatcatc < 1:253866/100‑1 (MQ=255) tttCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGc > 2:754685/1‑100 (MQ=255) tCAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCa < 1:1394726/100‑1 (MQ=255) cAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAg > 2:1181898/1‑100 (MQ=255) cAGCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGAGAGCAGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAg < 2:589035/100‑1 (MQ=255) gCCGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACataatctggctgat > 1:1531293/1‑92 (MQ=255) cGCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTa > 1:1553394/1‑100 (MQ=255) gCTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTAt > 2:680119/1‑100 (MQ=255) cTTGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATg < 2:1371838/100‑1 (MQ=255) ttGCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGTTCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATgg < 1:454854/100‑1 (MQ=255) gCAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAg > 2:1292498/1‑100 (MQ=255) gCAGGCACAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAg > 2:430414/1‑100 (MQ=255) cAGGCTCAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGt > 2:1539657/1‑100 (MQ=255) | GCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAAC‑GGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGT > NC_000913/2281183‑2281373 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |