| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,436,937 | A→T | D305E (GAT→GAA) | pdxB ← | erythronate‑4‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,436,937 | 0 | A | T | 97.5% | 279.7 / ‑2.6 | 80 | D305E (GAT→GAA) | pdxB | erythronate‑4‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (40/38); total (41/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
ATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCTATA > NC_000913/2436841‑2437032 | aTCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCg < 1:5614/100‑1 (MQ=255)aTCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCg > 1:70111/1‑100 (MQ=255)aTCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCg < 1:1334525/100‑1 (MQ=255)aTCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCg > 2:1852599/1‑100 (MQ=255)aTCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCg < 2:1981975/100‑1 (MQ=255) cGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAg < 2:621812/100‑1 (MQ=255) cGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAg > 2:461714/1‑100 (MQ=255) gAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGc > 1:350810/1‑100 (MQ=255) gAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGc < 1:346067/100‑1 (MQ=255) aaCTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCg < 1:1540802/100‑1 (MQ=255) aaCTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCg > 1:816774/1‑100 (MQ=255) cTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCggg > 1:1283721/1‑100 (MQ=255) tCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGc < 1:1635519/100‑1 (MQ=255) tCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGc > 1:297044/1‑100 (MQ=255) cACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGcc > 2:1947874/1‑100 (MQ=255) cACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGcc > 2:600977/1‑100 (MQ=255) cGGTATCCCGGCGACCTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGc < 1:2011833/100‑1 (MQ=255) gTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAg < 2:1459825/100‑1 (MQ=255) tATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGc < 1:1209128/100‑1 (MQ=255) cccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTa > 1:1776919/1‑100 (MQ=255) ccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTaa < 2:1360238/100‑1 (MQ=255) ccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTaa > 2:2029099/1‑100 (MQ=255) gCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTGCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGc < 2:1257323/100‑1 (MQ=255) cGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCg < 2:271998/100‑1 (MQ=255) cGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCg < 1:657454/100‑1 (MQ=255) aCTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGAc > 2:1379347/1‑100 (MQ=255) aCTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGAc < 2:19117/100‑1 (MQ=255) aCTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGAc > 1:639035/1‑100 (MQ=255) cTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGAcc > 2:318986/1‑100 (MQ=255) cGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAAct > 2:1146609/1‑100 (MQ=255) cGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCGAGCGGGCCATGCAGCGTAATGCGACCAAAct > 1:1594035/1‑100 (MQ=255) gCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTgg < 1:1227238/100‑1 (MQ=255) gCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTgg > 2:842709/1‑100 (MQ=255) cGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgc > 1:478868/1‑100 (MQ=255) cGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgc < 1:1910915/100‑1 (MQ=255) gTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgcgc > 2:1663696/1‑100 (MQ=255) gCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcag < 1:600979/100‑1 (MQ=255) acGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcag < 1:760215/99‑1 (MQ=255) gTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCCGCGTCGGTTGTTCGAGCGGGCCCTGCAGCGCAATGCGACCAGAc > 2:577477/1‑88 (MQ=255) gTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggc < 2:1470639/100‑1 (MQ=255) gTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggc > 2:550786/1‑100 (MQ=255) aTCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGt > 1:376047/1‑100 (MQ=255) gcggcgCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCACACTCTGGCGCAGGCAGTAAt > 2:1949035/1‑100 (MQ=255) cggcgCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATg < 2:282512/100‑1 (MQ=255) cggcgCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATg < 2:816771/100‑1 (MQ=255) cggcgCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATg < 2:1579108/100‑1 (MQ=255) cgcACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTAt > 1:610929/1‑100 (MQ=255) tcacaTCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATc < 2:1980675/99‑1 (MQ=255) cacaTCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTAtac < 1:1379356/100‑3 (MQ=255) cacaTCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATca > 1:1980693/1‑99 (MQ=255) acaTCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCa < 1:405280/100‑1 (MQ=255) catcatACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAg > 1:1622933/1‑100 (MQ=255) atcatACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAgc < 1:972134/100‑1 (MQ=255) catACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAgcgc < 1:821371/100‑1 (MQ=255) tACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCaa > 1:867414/1‑100 (MQ=255) acacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGGAGGCAGTAATGTATCCAGCGCAAc > 2:241241/1‑100 (MQ=255) acacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAAc < 1:1550522/100‑1 (MQ=255) cacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACg > 1:1736876/1‑100 (MQ=255) cacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACg > 1:342236/1‑100 (MQ=255) cacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACg < 1:550788/100‑1 (MQ=255) ccAAATGCACCAGCCTTTTCAGCGTGGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCTAGAGCAACGTg > 2:576815/1‑100 (MQ=255) ccAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTg > 2:793209/1‑100 (MQ=255) ccAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTg > 2:972420/1‑100 (MQ=255) gCACCAGCCTTTTCAGCATCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTc > 2:827779/1‑100 (MQ=255) ccAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATg > 1:1262361/1‑100 (MQ=255) cAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGc < 2:297043/100‑1 (MQ=255) cAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGc < 1:1346788/100‑1 (MQ=255) aGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGcc > 2:896713/1‑100 (MQ=255) aGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGcc < 2:350809/100‑1 (MQ=255) aGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGcc < 2:376046/100‑1 (MQ=255) aGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGcc > 2:48289/1‑100 (MQ=255) gCCTTTTCAGCGTCGCTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGccc < 1:2011753/100‑1 (MQ=255) cTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCaa > 2:909831/1‑100 (MQ=255) cTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCa < 1:793212/99‑1 (MQ=255) ttttCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAAt < 2:261537/100‑1 (MQ=255) tCAGCGTAGGTAGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATaaa > 2:1075181/1‑100 (MQ=255) cAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAAc > 2:561567/1‑100 (MQ=255) gTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGc < 1:2029117/100‑1 (MQ=255) tCGTTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCt < 2:1577022/100‑1 (MQ=255) ggTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCtat > 1:1076927/1‑100 (MQ=255) gttgttCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCtata < 1:827782/100‑1 (MQ=255) gttgttCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCtata > 2:1764143/1‑100 (MQ=255) | ATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCTATA > NC_000913/2436841‑2437032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |