Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,817,931 T→C intergenic (‑71/+128) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,817,9310TC99.0% 359.7 / ‑4.5 102intergenic (‑71/+128)argQ/argZtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (56/45);  total (57/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 102 total aligned reads displayed.

CTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTC  >  NC_000913/2817836‑2818026
                                                                                               |                                                                                               
ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCgg                                                                                             >  1:1270572/1‑100 (MQ=255)
ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCgg                                                                                             >  2:1715361/1‑100 (MQ=255)
ctctATCCAGCTGAGCTACGGATACATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCgg                                                                                             >  1:1731906/1‑100 (MQ=255)
 tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGTACCCggg                                                                                            >  2:1732932/1‑100 (MQ=255)
  ctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGgga                                                                                           <  2:1372012/100‑1 (MQ=255)
   tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGCTGCACCCCggag                                                                                          >  2:1902756/1‑100 (MQ=255)
    aTCCAGCTGAGCTACGGATGCATCGGAAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGggagg                                                                                         <  1:158401/100‑1 (MQ=255)
     tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGggagga                                                                                        <  2:1039581/100‑1 (MQ=255)
      ccAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGGCATATCAATTAAGAGATGGTGCACCCGGGAGGAt                                                                                       <  2:2007367/100‑1 (MQ=255)
       cAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGAtt                                                                                      <  1:1347116/100‑1 (MQ=255)
       cAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGAtt                                                                                      >  1:1285455/1‑100 (MQ=255)
         gCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTAc                                                                                    <  1:1332281/100‑1 (MQ=255)
         gCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTAc                                                                                    <  2:1976080/100‑1 (MQ=255)
            gAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCg                                                                                 >  2:1611771/1‑100 (MQ=255)
            gAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCg                                                                                 <  1:484293/100‑1 (MQ=255)
             aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATCCCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGc                                                                                <  2:925751/100‑1 (MQ=255)
             aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGc                                                                                >  2:165753/1‑100 (MQ=255)
                tACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCtt                                                                             >  1:1778735/1‑100 (MQ=255)
                 aCGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTg                                                                            >  2:1486887/1‑100 (MQ=255)
                   ggATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCg                                                                          >  2:827712/1‑100 (MQ=255)
                     aTGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGc                                                                        <  2:1655885/100‑1 (MQ=255)
                     aTGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGc                                                                        <  2:623353/100‑1 (MQ=255)
                      tGCATCGTGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCt                                                                       <  2:1102167/100‑1 (MQ=255)
                       gCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTc                                                                      <  1:1981498/100‑1 (MQ=255)
                        cATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCt                                                                       >  2:371792/1‑98 (MQ=255)
                        cATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                     >  2:1915874/1‑100 (MQ=255)
                         aTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGc                                                                    >  1:264668/1‑100 (MQ=255)
                         aTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGc                                                                    >  1:240017/1‑100 (MQ=255)
                          tCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGcc                                                                   >  1:924769/1‑100 (MQ=255)
                          gatctaaaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGcc                                                                   <  1:350816/95‑1 (MQ=255)
                           cGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGccc                                                                  >  1:166953/1‑100 (MQ=255)
                           cGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGATGCACCCGGGAGGATTACTCGCCTTGCGGCTCGccc                                                                  >  1:796473/1‑100 (MQ=255)
                            gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCt                                                                 >  1:50374/1‑100 (MQ=255)
                              gAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                               >  2:1213557/1‑100 (MQ=255)
                               aaaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGccagatc                                                              >  2:350815/1‑95 (MQ=255)
                                aaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCgg                                                             >  1:263526/1‑100 (MQ=255)
                                aaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCgg                                                             >  2:589241/1‑100 (MQ=255)
                                 aaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCggg                                                            <  1:1554811/100‑1 (MQ=255)
                                   cTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGcc                                                          <  1:125643/100‑1 (MQ=255)
                                   cTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGcc                                                          <  1:403114/100‑1 (MQ=255)
                                       ttCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTg                                                      >  2:681975/1‑100 (MQ=255)
                                        tCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGc                                                     <  1:16617/100‑1 (MQ=255)
                                         cTACTGCTGATATTGATGCCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCt                                                    >  2:1722226/1‑100 (MQ=255)
                                         cTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCt                                                    >  1:1622561/1‑100 (MQ=255)
                                            ctgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCttt                                                  <  1:946147/99‑3 (MQ=255)
                                            ctgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                 >  1:1793191/1‑100 (MQ=255)
                                            ctgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGACGTTGCTaaa                                                 >  2:1339600/1‑100 (MQ=255)
                                             tgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAg                                                <  2:708989/100‑1 (MQ=255)
                                              gctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGc                                               >  2:781195/1‑100 (MQ=255)
                                              gctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGc                                               <  1:1213563/100‑1 (MQ=255)
                                              gctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGc                                               <  2:1170044/100‑1 (MQ=255)
                                               ctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCa                                              >  2:629746/1‑100 (MQ=255)
                                               ctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCa                                              <  1:1611784/100‑1 (MQ=255)
                                                  atatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACg                                           <  2:1159745/100‑1 (MQ=255)
                                                   tatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGt                                          >  2:689847/1‑100 (MQ=255)
                                                    atTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGtt                                         >  1:258020/1‑100 (MQ=255)
                                                     ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTTAGCAACggcc                                        <  1:1670205/100‑4 (MQ=14)
                                                     ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTa                                        <  1:781198/100‑1 (MQ=255)
                                                      tGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTTAGCAACggccc                                       <  2:1546281/100‑5 (MQ=11)
                                                        aTACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatca                                     <  1:978095/100‑1 (MQ=255)
                                                          aCCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcatc                                   >  1:1450282/1‑100 (MQ=255)
                                                          aCCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcatc                                   <  2:882901/100‑1 (MQ=255)
                                                          aCCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcatc                                   <  1:576514/100‑1 (MQ=255)
                                                           ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTTAGCAACggcccgaagg                                  <  2:1526831/100‑10 (MQ=11)
                                                            cGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTTAGCAACggcccgaaggg                                 <  2:264667/100‑11 (MQ=14)
                                                            cGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTTAGCAACggcccgaaggg                                 <  2:240016/100‑11 (MQ=11)
                                                                  taAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGc                           >  2:458139/1‑100 (MQ=255)
                                                                   aaaGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGcc                          >  2:590084/1‑100 (MQ=255)
                                                                   aaaGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGcc                          >  1:825799/1‑100 (MQ=255)
                                                                   aaaGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGcc                          >  2:162556/1‑100 (MQ=255)
                                                                     aGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTg                        >  2:1509066/1‑100 (MQ=255)
                                                                        cATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCgg                     >  2:340537/1‑100 (MQ=255)
                                                                        cATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCgg                     >  2:623436/1‑100 (MQ=255)
                                                                         atatCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                    >  2:1510529/1‑100 (MQ=255)
                                                                         atatCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                    <  2:924765/100‑1 (MQ=255)
                                                                          tatCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCTTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCTACGTTATCATCCCGGGTGcgccatctc                   >  2:947085/1‑92 (MQ=11)
                                                                            tCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTaa                 >  1:487619/1‑100 (MQ=255)
                                                                             cAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAt                >  1:492493/1‑100 (MQ=255)
                                                                             cAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAt                >  2:505766/1‑100 (MQ=255)
                                                                               aTTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTc              >  1:74213/1‑100 (MQ=255)
                                                                               aTTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTc              >  2:1125303/1‑100 (MQ=255)
                                                                               aTTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTc              >  1:822528/1‑100 (MQ=255)
                                                                                ttAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTcc             <  2:1913131/100‑1 (MQ=255)
                                                                                  aaGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc           >  2:793459/1‑100 (MQ=255)
                                                                                  aaGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc           >  1:600914/1‑100 (MQ=255)
                                                                                  aaGAGATGGTGCACCCGGGAGAATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc           <  2:604167/100‑1 (MQ=255)
                                                                                   agagATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt          <  1:843596/100‑1 (MQ=255)
                                                                                     agaTGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc        <  2:258019/100‑1 (MQ=255)
                                                                                      gaTGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAcc       <  1:1722239/100‑1 (MQ=255)
                                                                                      gaTGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc        <  1:165754/99‑1 (MQ=255)
                                                                                       aTGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACct      >  2:1603952/1‑100 (MQ=255)
                                                                                        tGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACctc     >  1:764452/1‑100 (MQ=255)
                                                                                         ggTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCACCGCTCTCATCCCTAGTGCCTGCGGTTAATTCCGCTACctc     >  2:387506/1‑99 (MQ=17)
                                                                                          gTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCtt   >  2:255906/1‑100 (MQ=255)
                                                                                           tGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCTGCTACCTCTTc  >  2:1923503/1‑100 (MQ=255)
                                                                                           tGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc  <  2:1270566/100‑1 (MQ=255)
                                                                                           tGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc  <  2:1450273/100‑1 (MQ=255)
                                                                                           tGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc  <  1:589243/100‑1 (MQ=255)
                                                                                           tGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc  <  1:1902774/100‑1 (MQ=255)
                                                                                           tGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAATGCCTGCGGTTAATTCCGCTACCTCTTc  <  2:1778720/100‑1 (MQ=255)
                                                                                               |                                                                                               
CTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTC  >  NC_000913/2817836‑2818026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: