Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,887,352 | T→A | intergenic (‑133/+226) | ygcB ← / ← cysH | Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,887,352 | 0 | T | A | 98.8% | 298.9 / ‑3.9 | 84 | intergenic (‑133/+226) | ygcB/cysH | Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent |
Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (49/34); total (49/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 |
TCAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTC > NC_000913/2887257‑2887447 | tCAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGttt > 2:1668436/1‑100 (MQ=255) tCAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGttt > 1:1575696/1‑100 (MQ=255) aGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTgg > 2:1182980/1‑100 (MQ=255) gCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTggg > 2:752732/1‑100 (MQ=255) ggCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTc > 1:1212238/1‑100 (MQ=255) gCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCg < 1:752735/100‑1 (MQ=255) tATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGaa > 2:768866/1‑100 (MQ=255) tATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGaa > 1:1624538/1‑100 (MQ=255) tttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACg > 1:99816/1‑100 (MQ=255) ttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGc > 2:600794/1‑100 (MQ=255) tttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCt > 2:381011/1‑100 (MQ=255) ttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTg < 1:986657/100‑1 (MQ=255) tCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTgg > 1:17300/1‑100 (MQ=255) cGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTACCATTCGAATAGTTTGGGTTCGAACGCTGGc > 2:1817162/1‑100 (MQ=255) cGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGc > 2:62343/1‑100 (MQ=255) tgctgcTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTc < 1:1139218/100‑1 (MQ=255) gctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCa > 2:1933645/1‑100 (MQ=255) gctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCa > 1:1506549/1‑100 (MQ=255) tgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAgg < 1:875001/100‑1 (MQ=255) tgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAgg < 2:1367478/100‑1 (MQ=255) tgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAgg > 2:833721/1‑100 (MQ=255) gctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGt > 2:435573/1‑100 (MQ=255) cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGa < 2:17300/100‑1 (MQ=255) aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGAt < 2:1914162/100‑1 (MQ=255) aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGAt < 2:955068/100‑1 (MQ=255) aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGAt > 1:618425/1‑100 (MQ=255) tAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATa > 1:1437762/1‑100 (MQ=255) aaaTATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTTGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAg < 2:1262614/100‑1 (MQ=255) tatCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGaaa < 1:1855231/100‑1 (MQ=255) tCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAata < 1:768869/100‑1 (MQ=255) gAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATc < 1:62343/100‑1 (MQ=255) gAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATc > 2:371455/1‑100 (MQ=255) aaTTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCg < 1:1668449/100‑1 (MQ=255) aaTTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCg < 1:1815418/100‑1 (MQ=255) aaTTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCg < 1:133345/100‑1 (MQ=255) aTTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGc > 2:856205/1‑100 (MQ=255) tACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCt < 1:1533657/100‑1 (MQ=255) tACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCt > 2:1675092/1‑100 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCTAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTg < 2:758486/100‑1 (MQ=255) cAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTgg > 1:1330529/1‑100 (MQ=255) gTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTgggg > 2:275738/1‑100 (MQ=255) gATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCt > 1:1841362/1‑100 (MQ=255) gATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCt > 2:1147171/1‑100 (MQ=255) taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTg < 2:41092/100‑1 (MQ=255) taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTg > 1:1814092/1‑100 (MQ=255) cacaTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTc < 2:540226/100‑1 (MQ=255) cacaTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTc > 1:543894/1‑100 (MQ=255) cacaTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTc > 2:1591082/1‑100 (MQ=255) cacaTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTc > 1:772489/1‑100 (MQ=255) caTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTcta > 2:1242307/1‑98 (MQ=255) caTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCa > 2:102601/1‑100 (MQ=255) caTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCa < 2:1112434/100‑1 (MQ=255) gTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCt > 2:459354/1‑100 (MQ=255) gTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCt < 2:772486/100‑1 (MQ=255) gTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCt < 1:381012/100‑1 (MQ=255) tCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTg < 1:1675105/100‑1 (MQ=255) cTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCTAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCt > 1:1633735/1‑98 (MQ=255) cTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTgg < 1:1821184/100‑1 (MQ=255) tGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGaa > 1:726224/1‑100 (MQ=255) tGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGaa > 1:1901628/1‑100 (MQ=255) ccTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAAcc > 1:488338/1‑100 (MQ=255) cTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCt > 1:339214/1‑100 (MQ=255) cccTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcg > 2:1041708/1‑100 (MQ=255) cccTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcg > 1:1301920/1‑100 (MQ=255) ccTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgc > 2:1890123/1‑100 (MQ=255) cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg < 1:275739/100‑1 (MQ=255) cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg < 2:1624525/100‑1 (MQ=255) cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg < 2:488337/100‑1 (MQ=255) cTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTcgcg < 2:1212232/100‑1 (MQ=255) agaagaGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCt < 1:600796/100‑1 (MQ=255) agaagaGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCt < 2:1437753/100‑1 (MQ=255) agaagaGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCt < 1:371456/100‑1 (MQ=255) aagaGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTa < 1:193942/100‑1 (MQ=255) cTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCa > 1:838615/1‑100 (MQ=255) aCATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGAc < 1:102602/100‑1 (MQ=255) cATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGAc > 2:1841772/1‑99 (MQ=255) cATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACa > 2:587146/1‑100 (MQ=255) cATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACa > 2:1698462/1‑100 (MQ=255) cATTCGAATAGTTTGGGTTCGAACGCTGCCCTCAGGTTGATCGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGACTGCTTAACGCCAGAc > 2:1002834/1‑99 (MQ=255) tCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGcc < 1:834838/100‑1 (MQ=255) tCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGcc < 2:813083/100‑1 (MQ=255) cGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGcca > 1:813086/1‑99 (MQ=255) gAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTc > 2:581607/1‑100 (MQ=255) gAATAGTTTGGGTTCGAACGCTGGCCTCAGATTCATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCAAGACAGCCTc > 2:1342985/1‑100 (MQ=255) | TCAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTC > NC_000913/2887257‑2887447 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |