Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 265,810 T→A Q93L (CAG→CTG)  ykfB ← CP4‑6 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913265,8100TA100.0% 296.8 / NA 83Q93L (CAG→CTG) ykfBCP4‑6 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (41/42);  total (41/42)

TCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAA  >  NC_000913/265717‑265905
                                                                                             |                                                                                               
tCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGATGGAATAATGTACTGCAGGTGAt                                                                                           <  2:1189502/100‑1 (MQ=255)
tCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGAt                                                                                           <  1:2000173/100‑1 (MQ=255)
 cGAACTGCCCGGCCTCAGAGGTATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATc                                                                                          <  2:1595014/100‑1 (MQ=255)
   aaCTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCa                                                                                        <  1:161287/100‑1 (MQ=255)
    aCTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCAt                                                                                       >  1:1010292/1‑100 (MQ=255)
    aCTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCAt                                                                                       >  1:53274/1‑100 (MQ=255)
     cTGCCCGGCCTCAGAGATATTGCTGATGAAGTGAAACCATGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATc                                                                                      <  2:1432984/100‑1 (MQ=255)
     cTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATc                                                                                      <  2:29836/100‑1 (MQ=255)
       gCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCAc                                                                                    >  2:421728/1‑100 (MQ=255)
         ccGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTa                                                                                  >  2:801536/1‑100 (MQ=255)
            gCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACgg                                                                               <  1:792319/100‑1 (MQ=255)
             ccTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGt                                                                              <  2:1867262/100‑1 (MQ=255)
             ccTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGt                                                                              >  1:1189574/1‑100 (MQ=255)
              cTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTg                                                                             >  2:19316/1‑100 (MQ=255)
                cAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGAt                                                                           >  1:1743849/1‑100 (MQ=255)
                  gagaTATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGAtag                                                                         <  2:1044424/100‑1 (MQ=255)
                  gagaTATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCAAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGAtag                                                                         >  2:416551/1‑100 (MQ=255)
                       atTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAgtg                                                                    <  2:946401/100‑1 (MQ=255)
                       atTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAgtg                                                                    <  1:174450/100‑1 (MQ=255)
                        ttGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAgtg                                                                    >  1:1938531/1‑99 (MQ=255)
                            tgatgaAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTgtcgtc                                                               >  1:1128403/1‑100 (MQ=255)
                             gatgaAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCa                                                              >  2:1867205/1‑100 (MQ=255)
                             gatgaAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCa                                                              >  2:2551/1‑100 (MQ=255)
                                 aaGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGt                                                          >  2:445643/1‑100 (MQ=255)
                                 aaGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGt                                                          <  1:920785/100‑1 (MQ=255)
                                  aGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTg                                                         <  2:849115/100‑1 (MQ=255)
                                  aGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTg                                                         >  2:138961/1‑100 (MQ=255)
                                  aGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTg                                                         >  1:991233/1‑100 (MQ=255)
                                   gTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGc                                                        >  2:1582931/1‑100 (MQ=255)
                                   gTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGc                                                        >  1:503036/1‑100 (MQ=255)
                                   gTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGc                                                        <  1:573028/100‑1 (MQ=255)
                                    tCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCg                                                       >  1:899620/1‑100 (MQ=255)
                                     cAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGa                                                      >  1:101632/1‑100 (MQ=255)
                                     cAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGa                                                      >  2:976495/1‑100 (MQ=255)
                                     cAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAAGTTAGCCATGACTACGGTGATAGGAGTGTCGTCATCGTGCGa                                                      >  2:1431249/1‑100 (MQ=255)
                                       aaCCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATCGTAGTGTCGTCATCGTGCGAAc                                                    <  2:1189568/100‑1 (MQ=255)
                                       aaCCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAAc                                                    <  2:1695114/100‑1 (MQ=255)
                                           aGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGAc                                                <  2:586791/100‑1 (MQ=255)
                                           aGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGAc                                                >  2:1769268/1‑100 (MQ=255)
                                            ggCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGAcc                                               <  1:377797/100‑1 (MQ=255)
                                             gCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCa                                              <  2:503035/100‑1 (MQ=255)
                                                ttATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATc                                            <  1:1704485/99‑1 (MQ=255)
                                                ttATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCt                                           >  1:370439/1‑100 (MQ=255)
                                                  aTCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTg                                         <  1:750409/100‑1 (MQ=255)
                                                    cGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTc                                       >  2:318459/1‑100 (MQ=255)
                                                     gCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCa                                      >  2:835638/1‑100 (MQ=255)
                                                     gCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCa                                      >  1:1906766/1‑100 (MQ=255)
                                                     gCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCa                                      >  2:106790/1‑100 (MQ=255)
                                                       cGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATc                                    <  1:1046252/100‑1 (MQ=255)
                                                        gTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCg                                   <  2:370438/100‑1 (MQ=255)
                                                         ttACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCgg                                  <  1:976499/100‑1 (MQ=255)
                                                         ttACGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCgg                                  <  1:1319923/100‑1 (MQ=255)
                                                           aCGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCa                                <  2:1020684/100‑1 (MQ=255)
                                                            cGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCaa                               >  1:253245/1‑100 (MQ=255)
                                                            cGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCaa                               >  1:1313366/1‑100 (MQ=255)
                                                            cGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCaa                               >  1:1479662/1‑100 (MQ=255)
                                                            cGCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCaa                               >  2:325105/1‑100 (MQ=255)
                                                             gCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAg                              <  2:331301/100‑1 (MQ=255)
                                                             gCCAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAg                              <  2:1012859/100‑1 (MQ=255)
                                                              cccgCCCACGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTCGTGTCGTCACCGTGCGAAATGACCATCTTGTCATCGGCAAgg                             <  1:1431258/97‑1 (MQ=255)
                                                              ccAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAgg                             <  1:1415606/100‑1 (MQ=255)
                                                               cAGCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAggg                            <  2:53274/100‑1 (MQ=255)
                                                                 gCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCTTGCGAACTGACCATCTTGTCATCGGCAAGGGTa                          <  2:1339390/100‑1 (MQ=255)
                                                                 gCCCAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTa                          <  2:965181/100‑1 (MQ=255)
                                                                   ccAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTaag                        >  1:1577723/1‑100 (MQ=255)
                                                                   ccAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTaag                        >  1:777963/1‑100 (MQ=255)
                                                                    cAGGCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTaaga                       >  2:320181/1‑100 (MQ=255)
                                                                      ggCTGGACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTaagaaa                     <  1:241988/100‑1 (MQ=255)
                                                                          ggACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACt                 >  2:625821/1‑100 (MQ=255)
                                                                          ggACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACt                 >  2:1497187/1‑100 (MQ=255)
                                                                           gagggAATAATGTACTGCAGGGGATCGATGACTAGGGTGATAGTAGTGGCGTCATGGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTg                <  2:2015339/97‑1 (MQ=255)
                                                                           gACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTg                <  1:1582942/100‑1 (MQ=255)
                                                                           gACGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTg                >  2:1235657/1‑100 (MQ=255)
                                                                            aCGGAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGa               >  1:835565/1‑100 (MQ=255)
                                                                               gAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATg            >  2:1012629/1‑100 (MQ=255)
                                                                               gAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATg            <  2:628355/100‑1 (MQ=255)
                                                                               gAATAATGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATg            >  2:1289859/1‑100 (MQ=255)
                                                                                aataatGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATg            <  1:138962/99‑1 (MQ=255)
                                                                                 ataatGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCt          <  1:19316/100‑1 (MQ=255)
                                                                                    atGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGAt       >  2:839469/1‑100 (MQ=255)
                                                                                     tGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATa      <  1:421729/100‑1 (MQ=255)
                                                                                     tGTACTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATa      <  2:1743835/100‑1 (MQ=255)
                                                                                        aCTGCAGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGaa   <  1:320741/100‑1 (MQ=255)
                                                                                         ctgcagGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGaaa  >  2:245232/1‑100 (MQ=255)
                                                                                         ctgcagGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGaaa  >  2:63913/1‑100 (MQ=255)
                                                                                             |                                                                                               
TCGAACTGCCCGGCCTCAGAGATATTGCTGATGAAGTCAAACCAGGCGTTATCGCCGTTACGCCAGCCCAGGCTGGACGGAATAATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAA  >  NC_000913/265717‑265905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: