| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,582,272 | G→A | C137Y (TGT→TAT) | yhhZ → | putative Hcp1 family polymorphic toxin protein; putative colicin‑like DNase/tRNase activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,582,272 | 0 | G | A | 98.8% | 289.7 / ‑4.5 | 83 | C137Y (TGT→TAT) | yhhZ | putative Hcp1 family polymorphic toxin protein; putative colicin‑like DNase/tRNase activity |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (39/42); total (40/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAA > NC_000913/3582175‑3582368 | cAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATc < 2:1817464/100‑1 (MQ=255)cAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATc > 1:1409274/1‑100 (MQ=255) cTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAAc > 2:1391228/1‑100 (MQ=255) aaGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACAt > 1:1645801/1‑100 (MQ=255) aaGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTATATCaa > 2:1631677/1‑97 (MQ=255) gagGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCt < 1:293026/100‑1 (MQ=255) cgcTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATc > 2:1447026/1‑100 (MQ=255) gcTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATcn < 2:1409265/100‑2 (MQ=255) cTTTTTTATCGGCTATTCATCACCAGATCATTGAAATCCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGc < 1:1944657/100‑1 (MQ=255) ttttttATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCa > 2:481253/1‑100 (MQ=255) tttttATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCaa > 1:49047/1‑100 (MQ=255) tttATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAAt < 1:1160865/100‑1 (MQ=255) tttATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAAt < 1:387052/100‑1 (MQ=255) ttATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATa < 1:1981142/100‑1 (MQ=255) tCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCg < 2:1355113/100‑1 (MQ=255) cGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGa < 1:1270031/100‑1 (MQ=255) ggCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAg > 1:816962/1‑100 (MQ=255) ggCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAg > 2:727330/1‑100 (MQ=255) ggCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAg < 2:216103/100‑1 (MQ=255) gCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGt < 1:1450413/100‑1 (MQ=255) tATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTc > 2:563582/1‑100 (MQ=255) gatctatcaCCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTc < 2:1999754/95‑1 (MQ=255) aTTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCa < 2:2011294/100‑1 (MQ=255) atcaCCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAgatc > 1:1999772/1‑97 (MQ=255) aGATCATTGAAAACCAACTGGATACAGAAACAATGACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGc < 1:1607424/100‑1 (MQ=255) aGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGc < 1:1666562/100‑1 (MQ=255) aGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGc < 1:1391237/100‑1 (MQ=255) aGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGc < 1:1387326/100‑1 (MQ=255) aGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGc < 2:1529480/100‑1 (MQ=255) gATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCa > 2:1025696/1‑100 (MQ=255) aTCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCAc < 1:1485051/100‑1 (MQ=255) aTTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTcc > 1:1876274/1‑100 (MQ=255) aTTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTcc < 1:2033903/100‑1 (MQ=255) aTTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTc > 2:331974/1‑99 (MQ=255) ttGAAAACCTACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTccc > 2:1603605/1‑100 (MQ=255) ttGAAAACCAACTGGATACAGAAACAATAACAATTAGTTATGAATTTATCCCCTATCAAAATCTTATCGCAAATAACGAGTTCAGCTATTTGGCACTccc > 2:1037274/1‑100 (MQ=255) tGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCt > 2:1112639/1‑100 (MQ=255) gAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTg > 1:601476/1‑100 (MQ=255) gAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTg < 1:1101201/100‑1 (MQ=255) aaCCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGaaa > 1:1087198/1‑100 (MQ=255) aCCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGaaaa < 1:1354360/100‑1 (MQ=255) ccAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAAt < 2:1672316/100‑1 (MQ=255) cAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAAtt > 2:804706/1‑100 (MQ=255) aaCTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATta > 1:318514/1‑100 (MQ=255) cTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATtata < 1:65023/100‑1 (MQ=255) ggATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAAc < 1:873157/100‑1 (MQ=255) gATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAAcc < 1:462318/100‑1 (MQ=255) gATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAAcc < 1:1037279/100‑1 (MQ=255) gATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAAcc < 2:1175056/100‑1 (MQ=255) aTACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCg < 2:864331/100‑1 (MQ=255) tACAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCgt > 1:170701/1‑100 (MQ=255) cAGAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCgttt > 2:1233892/1‑100 (MQ=255) cttccgatctaCTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCgtttg < 1:509413/90‑1 (MQ=255) gAAACAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCgtttgt > 2:1030113/1‑100 (MQ=255) aaCAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCgtttgttt > 1:1277239/1‑100 (MQ=255) cAATAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGttttt > 1:1090986/1‑100 (MQ=255) tAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTAcc < 2:601474/100‑1 (MQ=255) tAACTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTAcc < 1:1603617/100‑1 (MQ=255) aaCTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCa < 2:78319/100‑1 (MQ=255) aCTATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCgtttgagatcggaag > 2:509412/1‑90 (MQ=255) tATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAAt > 2:667554/1‑100 (MQ=255) tATTAGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAAt > 2:2018465/1‑100 (MQ=255) aGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaa < 1:883656/100‑1 (MQ=255) aGTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaa > 2:205202/1‑100 (MQ=255) gTTATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaa > 2:1444734/1‑100 (MQ=255) ttATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaa > 2:492064/1‑100 (MQ=255) tATGAATTTATCCTCTCTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaaa < 2:1193202/100‑1 (MQ=255) tATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaaa < 1:727332/100‑1 (MQ=255) tATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaaa > 1:1507007/1‑100 (MQ=255) tATGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaaa > 2:1589871/1‑100 (MQ=255) aTGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaaaa > 1:1598271/1‑100 (MQ=255) aTGAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaaaaaa < 1:1233898/100‑1 (MQ=255) gAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAAcc < 1:1626117/100‑1 (MQ=255) gAATTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAAcc < 2:5272/100‑1 (MQ=255) aaTTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACca > 1:1452614/1‑100 (MQ=255) aaTTTATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACca < 2:1087193/100‑1 (MQ=255) tttATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACcaaa > 2:1340856/1‑100 (MQ=255) ttATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACcaaac < 1:1589883/100‑1 (MQ=255) tATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACcaaaca < 1:1447035/100‑1 (MQ=255) tATCCTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACcaaaca < 1:1445290/100‑1 (MQ=255) ccTCTATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAaat > 1:532782/1‑100 (MQ=255) tctATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAaataa > 1:381810/1‑100 (MQ=255) tctATCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAaataa > 2:1014399/1‑100 (MQ=255) | CAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAA > NC_000913/3582175‑3582368 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |