Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,622,796 T→G L1202R (CTG→CGG)  rhsB → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,622,7960TG98.8% 217.1 / NA 83L1202R (CTG→CGG) rhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base G (50/31);  minor base A (0/1);  total (51/32)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGATA  >  NC_000913/3622701‑3622892
                                                                                               |                                                                                                
tgatgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAt                                                                                              >  1:913886/1‑100 (MQ=25)
 gatgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAtt                                                                                             <  2:238595/100‑1 (MQ=18)
  atgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTa                                                                                            <  1:1664397/100‑1 (MQ=18)
   tgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTAc                                                                                           >  2:1347853/1‑100 (MQ=17)
    gaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACa                                                                                          <  2:967042/100‑1 (MQ=18)
    gaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACa                                                                                          >  1:1085665/1‑100 (MQ=18)
     aaTGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACaa                                                                                         >  2:1864012/1‑100 (MQ=18)
        ggggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCg                                                                                      >  1:544199/1‑100 (MQ=18)
        ggggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCg                                                                                      >  1:1799303/1‑100 (MQ=18)
          ggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGcc                                                                                    >  1:73981/1‑100 (MQ=18)
           gCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCa                                                                                   >  2:78243/1‑100 (MQ=18)
             aaCCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCAcc                                                                                 <  1:268329/100‑1 (MQ=18)
             aaCCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCAcc                                                                                 >  1:1088328/1‑100 (MQ=17)
              aCCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCg                                                                                >  1:1109859/1‑100 (MQ=18)
                ctgctgAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCt                                                                              <  1:1864029/100‑1 (MQ=18)
                 tgctgAATGAAGAGACCGCGCATCAGCTGCGGCAGCTTATCCGGCGGCTGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGc                                                                               <  2:1850982/98‑1 (MQ=11)
                 tgctgAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCta                                                                             >  2:801473/1‑100 (MQ=18)
                  gctgAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtat                                                                            >  1:6129/1‑100 (MQ=18)
                  gctgAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtat                                                                            >  1:1988488/1‑100 (MQ=18)
                    tgaatgaaGATAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGCGTCCGGCCGGTATTACAACCGCCACCGCtatta                                                                          <  2:1337104/100‑1 (MQ=255)
                    tgaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtatta                                                                          >  1:631711/1‑100 (MQ=18)
                    tgaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtatta                                                                          >  1:596818/1‑100 (MQ=18)
                     gaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtattat                                                                         >  1:288520/1‑100 (MQ=18)
                     gaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtattat                                                                         <  1:1017102/100‑1 (MQ=18)
                     gaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtattat                                                                         >  1:1007021/1‑100 (MQ=18)
                     gaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtatta                                                                          >  1:1593236/1‑99 (MQ=18)
                       atgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGa                                                                       >  1:1742351/1‑100 (MQ=18)
                       atgaaGAGAACCCGCATCAGCTGCAGCAGCTTAACCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGa                                                                       >  2:1226917/1‑100 (MQ=255)
                          aaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAccc                                                                    >  2:95881/1‑100 (MQ=18)
                           agagAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGTCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCg                                                                   >  2:1864461/1‑100 (MQ=255)
                           agagAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCg                                                                   >  2:1447876/1‑100 (MQ=18)
                                aCCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCa                                                              <  1:1215357/100‑1 (MQ=18)
                                 cccGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAg                                                             >  1:900227/1‑100 (MQ=18)
                                  ccGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAgg                                                            <  1:1321780/100‑1 (MQ=18)
                                   cGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAggg                                                           >  2:180840/1‑100 (MQ=17)
                                       tCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGa                                                       <  2:275439/100‑1 (MQ=18)
                                         aGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGata                                                     >  1:301062/1‑100 (MQ=17)
                                           cTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGatata                                                   >  1:90252/1‑100 (MQ=18)
                                             gcagcaGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATc                                                 <  2:73981/100‑1 (MQ=18)
                                                 cagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTc                                             <  2:913882/100‑1 (MQ=17)
                                                 cagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTc                                             <  1:801476/100‑1 (MQ=18)
                                                  agcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCa                                            >  2:522971/1‑100 (MQ=33)
                                                  agcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCa                                            >  2:359125/1‑100 (MQ=18)
                                                  agcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCa                                            >  2:359140/1‑100 (MQ=18)
                                                  agcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTACTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCa                                            >  2:1939762/1‑100 (MQ=255)
                                                   gcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAg                                           <  1:1226923/100‑1 (MQ=18)
                                                        tCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATcc                                      >  2:1852650/1‑100 (MQ=17)
                                                        tCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATcc                                      <  2:491576/100‑1 (MQ=17)
                                                        tCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATcc                                      <  1:522874/100‑1 (MQ=18)
                                                        tCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATcc                                      <  1:484113/100‑1 (MQ=18)
                                                        ctcGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATcc                                      <  1:120607/98‑1 (MQ=12)
                                                         ccGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCg                                     <  1:1264130/100‑1 (MQ=18)
                                                         ccGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCg                                     <  1:1447885/100‑1 (MQ=18)
                                                          cGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATc                                       >  2:120606/1‑97 (MQ=17)
                                                           ggCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCCCTCAGGATCCGAt                                   <  1:1370565/100‑1 (MQ=9)
                                                           ggCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGAt                                   <  1:95882/100‑1 (MQ=14)
                                                            gCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGAtt                                  >  1:1989459/1‑100 (MQ=11)
                                                                ccGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGAc                              >  1:264267/1‑100 (MQ=14)
                                                                 cGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACt                             >  1:557092/1‑100 (MQ=17)
                                                                  ggggCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTg                            >  2:1896801/1‑100 (MQ=17)
                                                                      cagcagTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgg                        >  2:727532/1‑100 (MQ=25)
                                                                          agTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgggggn                    <  2:1988470/100‑2 (MQ=255)
                                                                          agTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgggggg                    >  1:1300887/1‑100 (MQ=255)
                                                                             atgatgAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATg                 >  1:453177/1‑100 (MQ=255)
                                                                             atgatgAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATg                 >  1:2004922/1‑100 (MQ=255)
                                                                             atgatgAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATGATGACCCGCTGCAGGGGCGCTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATg                 >  2:1119544/1‑100 (MQ=255)
                                                                              tgatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATgg                <  2:1109854/100‑1 (MQ=255)
                                                                              tgatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATgg                >  1:290200/1‑100 (MQ=255)
                                                                              tgatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATgg                >  1:1839876/1‑100 (MQ=255)
                                                                               gatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGa               >  2:1630320/1‑100 (MQ=255)
                                                                               gatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGa               >  2:529404/1‑100 (MQ=255)
                                                                               gatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGa               <  2:900224/100‑1 (MQ=255)
                                                                                 tgaGGAGTCCGGCCGGTATTATAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATAGATCACTGAGGATCCGATTGGACTGAAGGGGGGATGGAAc             >  2:1431823/1‑100 (MQ=14)
                                                                                 tgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCCGGCTCCGATTGGACTGCAGGGGGGATGGAAc             >  1:697039/1‑100 (MQ=11)
                                                                                  gaggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAAcc            <  1:359126/100‑1 (MQ=255)
                                                                                  gaggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAAcc            <  1:359141/100‑1 (MQ=255)
                                                                                    ggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGGTGCAGGGGCGATATATCACTCAGGATCCGGTTGGACTGAAGGGGGGATGGAACCTg          >  2:384775/1‑100 (MQ=255)
                                                                                      agTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTa        <  1:1045462/100‑1 (MQ=255)
                                                                                        tCCGGCCAGTATGACAACCCCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATg      <  1:1793595/100‑1 (MQ=255)
                                                                                          cGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGa    <  1:727534/100‑1 (MQ=255)
                                                                                           ggCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGat   >  2:100907/1‑100 (MQ=255)
                                                                                           ggCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGa    <  2:1085660/99‑1 (MQ=255)
                                                                                            gCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGata  >  2:1628445/1‑100 (MQ=255)
                                                                                               |                                                                                                
TGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGATA  >  NC_000913/3622701‑3622892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑