Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,636,979	A→T	D144E (GAT→GAA)	yhiN ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,636,979	0	A	T	100.0%	198.1 / NA	56	D144E (GAT→GAA)	yhiN	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (30/26);  total (30/26)

TCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATCCACCAGCA  >  NC_000913/3636885‑3637073                                                                                               |                                                                                               tCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAg                                                                                           <  2:1800333/100‑1 (MQ=255)  cTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGc                                                                                          >  1:695059/1‑100 (MQ=255)     ataACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTg                                                                                       <  1:85816/100‑1 (MQ=255)      taACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGa                                                                                      >  1:221898/1‑100 (MQ=255)       aaCCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTgta                                                                                     >  2:1033869/1‑98 (MQ=255)        aCCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAg                                                                                    >  2:795548/1‑100 (MQ=255)          cAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAg                                                                                    <  2:1575492/98‑1 (MQ=255)          cAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGcc                                                                                  >  2:1897050/1‑100 (MQ=255)          cAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGcc                                                                                  >  1:1966754/1‑100 (MQ=255)          cAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGcc                                                                                  >  2:62942/1‑100 (MQ=255)           aaaCGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCt                                                                                 >  1:100034/1‑100 (MQ=255)               ggCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGttt                                                                             <  2:1909981/100‑1 (MQ=255)               ggCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGttt                                                                             <  2:695565/100‑1 (MQ=255)                    cgcgCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATcc                                                                        >  2:1951572/1‑100 (MQ=255)                    cgcgCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATcc                                                                        >  2:941545/1‑100 (MQ=255)                      cgcCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCtt                                                                      >  2:843238/1‑100 (MQ=255)                        cccAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCg                                                                    >  1:1624189/1‑100 (MQ=255)                         ccAGCCCCGGCATTGAAAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCg                                                                    <  2:778324/99‑1 (MQ=255)                          cAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGcc                                                                  <  1:1927537/100‑1 (MQ=255)                          cAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGcc                                                                  >  2:1565198/1‑100 (MQ=255)                                     ttGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCAc                                                       <  2:2013771/100‑1 (MQ=255)                                      tGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTGCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACt                                                      <  1:191088/100‑1 (MQ=255)                                             ccaccaGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTAc                                               >  1:527530/1‑100 (MQ=255)                                              caccaGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTa                                                <  2:1689107/98‑1 (MQ=255)                                               accaGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGc                                             <  1:875999/100‑1 (MQ=255)                                                ccaGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCa                                            >  1:979961/1‑100 (MQ=255)                                                 caGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCaa                                           >  1:705872/1‑100 (MQ=255)                                                 caGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCaa                                           >  1:530670/1‑100 (MQ=255)                                                   gTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATGCTTCGCCACACTCAGCACTTCGCTACGCAATc                                         >  2:1649629/1‑100 (MQ=255)                                                    tCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCt                                        <  1:1551234/100‑1 (MQ=255)                                                      gctaTGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGa                                      <  2:1212750/97‑1 (MQ=255)                                                         aTGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAgg                                   <  1:1246288/100‑1 (MQ=255)                                                           gACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTc                                 >  1:1750396/1‑100 (MQ=255)                                                            aCCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTca                                >  1:100563/1‑100 (MQ=255)                                                            aCCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTca                                <  1:793363/100‑1 (MQ=255)                                                            aCCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTca                                >  1:717844/1‑100 (MQ=255)                                                               aGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACAt                             >  1:1425178/1‑100 (MQ=255)                                                                gCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACAtt                            >  1:1839587/1‑100 (MQ=255)                                                                gCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACAtt                            <  1:34677/100‑1 (MQ=255)                                                                gCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACAtt                            >  2:424945/1‑100 (MQ=255)                                                                 cTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTg                           >  2:59463/1‑100 (MQ=255)                                                                   tttCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGcc                         <  1:941549/100‑1 (MQ=255)                                                                    ttCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGccc                        <  1:1951590/100‑1 (MQ=255)                                                                     tCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCt                       <  2:567599/100‑1 (MQ=255)                                                                      cGCAACCGACAGTCATGCTGTTCAGTTCAAGCGTGCAGCCTGTTTCACCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACAtt                            <  2:763534/94‑1 (MQ=255)                                                                       gCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTtc                     <  2:100033/100‑1 (MQ=255)                                                                             gACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCAc               >  1:1321175/1‑100 (MQ=255)                                                                                 gTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCAt           <  2:1966736/100‑1 (MQ=255)                                                                                 gTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCAt           <  1:42952/100‑1 (MQ=255)                                                                                  tCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATc          <  2:1513766/100‑1 (MQ=255)                                                                                     tGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATccac       >  2:1071258/1‑100 (MQ=255)                                                                                       ccGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATccacca     >  1:1767782/1‑100 (MQ=255)                                                                                       ccGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATccacca     >  1:1725217/1‑100 (MQ=255)                                                                                        cGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATCCACCAg    <  2:1624176/100‑1 (MQ=255)                                                                                          ttCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATCCACCAGCa  >  2:1915095/1‑100 (MQ=255)                                                                                          ttCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATCCACCAGCa  <  2:100562/100‑1 (MQ=255)                                                                                               |                                                                                               TCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATCCACCAGCA  >  NC_000913/3636885‑3637073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑