| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,950,468 | G→T | intergenic (+48/‑39) | ilvL → / → ilvX | ilvG operon leader peptide/uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,950,468 | 0 | G | T | 95.7% | 223.4 / 1.9 | 70 | intergenic (+48/‑39) | ilvL/ilvX | ilvG operon leader peptide/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (30/37); total (31/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01 | |||||||||||
TGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑GTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATG > NC_000913/3950371‑3950562 | tgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGATTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑ttt > 2:93111/1‑100 (MQ=255) gATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑tttt < 2:683786/100‑1 (MQ=255) ttattaTCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑tttttt < 2:906127/100‑1 (MQ=255) tattatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑Ttttttt > 1:1606682/1‑100 (MQ=255) attatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑tttttttt < 1:1426688/100‑1 (MQ=255) tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTg < 2:1668809/100‑1 (MQ=255) tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTg < 1:910561/100‑1 (MQ=255) taccccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTg < 1:675327/97‑1 (MQ=255) tctccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGa < 1:1539400/97‑1 (MQ=255) atCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTg > 2:1244872/1‑99 (MQ=255) tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACTAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGAc < 2:1273833/100‑1 (MQ=255) cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGAcc > 2:32055/1‑100 (MQ=255) ccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGaag > 2:1539391/1‑97 (MQ=255) ccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCt > 1:961832/1‑100 (MQ=255) cACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCtt < 2:849471/100‑1 (MQ=255) aCCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTa > 2:1857906/1‑100 (MQ=255) cggTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGAACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTa < 2:616414/97‑1 (MQ=255) cGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTaaa < 2:961828/100‑1 (MQ=255) gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTaaaa < 2:1446820/100‑1 (MQ=255) gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTaaaa < 1:1644489/100‑1 (MQ=255) tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTaaaaa > 1:1725398/1‑100 (MQ=255) gCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAAc < 2:1240422/100‑1 (MQ=255) cGGGGCTGCACTTAGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACa < 1:1092079/100‑1 (MQ=255) ggCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATaa > 1:1460644/1‑100 (MQ=255) gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAAc < 1:10890/100‑1 (MQ=255) tGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCg > 2:1135068/1‑100 (MQ=255) cACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCgag > 1:1047934/1‑100 (MQ=255) cACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCgag > 1:676311/1‑100 (MQ=255) aCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCgagg > 1:1416219/1‑100 (MQ=255) ttGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCgaggag < 1:205289/100‑1 (MQ=255) tGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGc > 2:1801788/1‑100 (MQ=255) gACGAGTAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAg < 1:606183/100‑1 (MQ=255) gACGAGCAAAGGCTTAGAGATCATGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCTGGGG‑GTCTTTGTTGATGTTAAAAACATTACCTTGGATCAg > 2:1396728/1‑100 (MQ=255) cGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGAc > 2:593777/1‑100 (MQ=255) cGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑GTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGAc < 2:57106/100‑1 (MQ=255) ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt > 2:101521/1‑100 (MQ=255) ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt > 1:2005815/1‑100 (MQ=255) ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt > 1:271858/1‑100 (MQ=255) aGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAAt < 2:1989985/100‑1 (MQ=255) ggCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGatcggaagag > 2:1487984/1‑91 (MQ=255) cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc < 2:1052789/100‑1 (MQ=255) cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGAACCAAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc < 2:1416210/100‑1 (MQ=255) ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc < 1:1963789/99‑1 (MQ=255) ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa < 2:1606670/100‑1 (MQ=255) tAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAg > 2:1876081/1‑100 (MQ=255) agagATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc > 2:649829/1‑100 (MQ=255) agaTCAAGCCTTACCGCCGCAAGGCCCCCGCGGCGAAAGGTCCGGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc < 1:2040122/99‑1 (MQ=255) agaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGca < 2:1725385/99‑1 (MQ=255) gaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcaca > 1:1417844/1‑100 (MQ=255) gaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAAAACAGAACAAGACAATGAATAACAGca > 1:1109326/1‑98 (MQ=255) aaGCCTTAACGAACTAAGGCCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAt < 1:972969/100‑1 (MQ=255) ccTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACCATGAAGAACAGCCCAAAATTCt > 2:904317/1‑100 (MQ=255) ccTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCt > 1:1496279/1‑100 (MQ=255) ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGt < 2:80732/100‑1 (MQ=255) aCGAACTAAGACCCCCGCACCGAGAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc < 1:32055/100‑1 (MQ=255) aCGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc < 2:1840974/100‑1 (MQ=255) aCGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc < 2:700804/100‑1 (MQ=255) aCGAACTAAGACCCCCGCACCGAAAGGTCC‑GGG‑GTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct < 1:63151/100‑1 (MQ=255) aaCTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa < 1:1801804/100‑1 (MQ=255) aaCTAAGACCCCCGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa > 1:1659338/1‑100 (MQ=255) cTAAGCCCCCCGCCCCGAGAGGGCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCaa < 1:1857923/99‑1 (MQ=255) cccccGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAg < 2:271857/100‑1 (MQ=255) ccccGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAgg > 2:226050/1‑100 (MQ=255) ccccGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAgg < 2:1659325/100‑1 (MQ=255) cccGCACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGa > 1:892117/1‑100 (MQ=255) cACCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACggg > 2:1548723/1‑100 (MQ=255) aCCGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACgggg < 2:300871/100‑1 (MQ=255) ccGAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGa > 1:473020/1‑100 (MQ=255) gAAAGGTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac < 2:1047929/100‑1 (MQ=255) gAAAGGTCCGGGG‑TTTTTTTTTGACCTAAAAAACATAACCGAGGAGCAGACAATGAAAAAAAAAAAAAAATTCTGTTTCTCAAGATTCAGGACGGGGa < 2:892114/98‑1 (MQ=255) gTCCGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaactaact < 2:1220514/100‑1 (MQ=255) ccGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAactaacta < 1:314833/99‑1 (MQ=255) ccGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTAt > 1:203690/1‑100 (MQ=255) cGGGG‑TTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCATAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATg < 2:1863975/100‑1 (MQ=255) | TGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGG‑GTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATG > NC_000913/3950371‑3950562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |