Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,523,304	G→T	P264H (CCT→CAT)	yjhG ←	putative dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,523,304	0	G	T	100.0%	264.8 / NA	75	P264H (CCT→CAT)	yjhG	putative dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/42);  total (33/42)

TCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGA  >  NC_000913/4523209‑4523396                                                                                                |                                                                                             tCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTc                                                                                          <  2:428172/100‑1 (MQ=255) tCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTc                                                                                          >  2:1625473/1‑100 (MQ=255)   gCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCAc                                                                                        >  1:111124/1‑100 (MQ=255)   gCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCAc                                                                                        >  2:1474174/1‑100 (MQ=255)        aTCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGaa                                                                                   >  2:1144943/1‑100 (MQ=255)           ggTGAGAATTTCCCGGGTGGTGATGCCTTTTTTTTTTTTTTTTAGCGCAGCTCGCGCGGAAGCTCTGTCGATCTCCCGCCACACATGCTCACCGGaa                                                                                   >  2:782733/1‑97 (MQ=255)           ggTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAggg                                                                                >  1:610985/1‑100 (MQ=255)            gTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAgggg                                                                               <  2:859732/100‑1 (MQ=255)             tGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGc                                                                              <  2:172005/100‑1 (MQ=255)              gagaATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGcc                                                                             <  2:1368866/100‑1 (MQ=255)                gaATTTCCCGGGTGGTGATGCCTTTTTTTTTTAGTTTTAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAg                                                                           >  2:804548/1‑100 (MQ=255)                gaATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAg                                                                           >  1:850789/1‑100 (MQ=255)                    ttCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCt                                                                       <  1:1765292/100‑1 (MQ=255)                    ttCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCt                                                                       <  1:97073/100‑1 (MQ=255)                     tCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTg                                                                      >  1:1472455/1‑100 (MQ=255)                     tCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTg                                                                      <  1:1654237/100‑1 (MQ=255)                      cccGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCCGGGCt                                                                       >  2:997502/1‑98 (MQ=255)                       ccGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGaa                                                                    >  2:210390/1‑100 (MQ=255)                        cGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAAt                                                                   <  2:1934990/100‑1 (MQ=255)                        cGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAAt                                                                   <  2:440539/100‑1 (MQ=255)                         gggTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAAtg                                                                  <  1:1555094/100‑1 (MQ=255)                            tggtgATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTgg                                                               <  1:1677765/100‑1 (MQ=255)                            tggtgATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTgg                                                               <  1:210391/100‑1 (MQ=255)                            tggtgATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTgg                                                               <  2:1043414/100‑1 (MQ=255)                             ggtgATGCCTTTTTTTTTTTTTTTTAGCGCAGCACGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGTGGCCAGGGCTGAATGTggg                                                              >  2:1579357/1‑100 (MQ=255)                             ggtgATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTggg                                                              <  2:591634/100‑1 (MQ=255)                              gtgATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGa                                                             <  2:181929/100‑1 (MQ=255)                               tgATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGAt                                                            >  2:270010/1‑100 (MQ=255)                               tgATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGAt                                                            <  1:1625486/100‑1 (MQ=255)                                 aTGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTg                                                          <  2:1285294/100‑1 (MQ=255)                                  tGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGc                                                         >  2:506122/1‑100 (MQ=255)                                  tGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGc                                                         <  2:635041/100‑1 (MQ=255)                                  tGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGc                                                         >  2:1527261/1‑100 (MQ=255)                                   gCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGcc                                                        <  1:1394968/100‑1 (MQ=255)                                       ttttGACTCAGGTTCAGCGGAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTc                                                    <  1:1853265/100‑1 (MQ=255)                                        tttGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTcc                                                   <  1:120075/100‑1 (MQ=255)                                        tttGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTcc                                                   >  2:899314/1‑100 (MQ=255)                                           gACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCaa                                                >  1:1240484/1‑100 (MQ=255)                                           gACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCaa                                                >  1:327264/1‑100 (MQ=255)                                           gACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCaa                                                >  1:20008/1‑100 (MQ=255)                                            aCTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAAt                                               >  2:307832/1‑100 (MQ=255)                                            aCTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAAt                                               <  2:1585522/100‑1 (MQ=255)                                             cTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATc                                              >  1:1536807/1‑100 (MQ=255)                                             cTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATc                                              >  1:119384/1‑100 (MQ=255)                                             cTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATc                                              >  1:1602287/1‑100 (MQ=255)                                             cTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATc                                              <  1:1548471/100‑1 (MQ=255)                                              tCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGACTGGCAATCCCacatt                                             <  1:1874700/100‑5 (MQ=255)                                                 ggTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTc                                          <  1:1144949/100‑1 (MQ=255)                                                  gTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCg                                         >  1:101743/1‑100 (MQ=255)                                                   ttCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCgg                                        <  2:1474643/100‑1 (MQ=255)                                                   ttCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCgg                                        <  1:858714/100‑1 (MQ=255)                                                   ttCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGCGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCg                                         >  2:2026082/1‑99 (MQ=255)                                                     cAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGcc                                      <  2:206304/100‑1 (MQ=255)                                                                gcgcgGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTgaga                           <  1:301949/100‑1 (MQ=255)                                                                 cgcgGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGAt                          <  2:497839/100‑1 (MQ=255)                                                                  gctgAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATg                         <  2:1383741/97‑1 (MQ=255)                                                                        gCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCgg                   <  2:119383/100‑1 (MQ=255)                                                                        gCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCgg                   <  1:569109/100‑1 (MQ=255)                                                                        gCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCgg                   >  1:617023/1‑100 (MQ=255)                                                                         ctctGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGc                  >  1:2021807/1‑100 (MQ=255)                                                                         ctctGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGc                  <  2:593738/100‑1 (MQ=255)                                                                          tctGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCa                 >  1:1477963/1‑100 (MQ=255)                                                                            tGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGt               <  1:804551/100‑1 (MQ=255)                                                                            tGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGt               >  1:1973784/1‑100 (MQ=255)                                                                             ccagagcttccGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTg              <  2:1719651/91‑1 (MQ=255)                                                                               cGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGcc            <  1:1373417/100‑1 (MQ=255)                                                                                    tcCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCaaaa       >  1:806434/1‑100 (MQ=255)                                                                                    tcCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCaaaa       >  2:1891123/1‑100 (MQ=255)                                                                                     cccGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCaaaaa      >  1:867558/1‑100 (MQ=255)                                                                                     cccGCCACACATGCTCACCGGAAGGGGCCAGGGATGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACGACCTGAGATGTCCCGGCAGTGCCCaaaaa      >  2:1225683/1‑100 (MQ=255)                                                                                       cGCCACACATGCTCGCCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAAtt    <  2:1906676/100‑1 (MQ=255)                                                                                       cGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAAtt    <  2:336622/100‑1 (MQ=255)                                                                                       cGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAAtt    <  2:20008/100‑1 (MQ=255)                                                                                        gCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTg   <  2:973594/100‑1 (MQ=255)                                                                                         ccACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGa  <  2:610983/100‑1 (MQ=255)                                                                                                |                                                                                             TCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGA  >  NC_000913/4523209‑4523396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑