Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,868,859 Δ1 bp 15.9% intergenic (+17/‑96) yeaG → / → yeaH protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,868,8590C.15.9% 416.0 / 82.5 145intergenic (+17/‑96)yeaG/yeaHprotein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base C (54/67);  new base . (9/14);  total (63/82)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

Only 100 of 156 total aligned reads displayed.

ACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGA  >  NC_000913/1868760‑1868958
                                                                                                   |                                                                                                    
aCCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTac                                                                                                      <  2:3303813/100‑1 (MQ=255)
  cGTATGATGGAAAAATGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTacac                                                                                                    <  2:3456142/100‑1 (MQ=255)
   gTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAcaca                                                                                                   >  1:851788/1‑100 (MQ=255)
     atgctgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATg                                                                                                 <  1:3318413/100‑1 (MQ=255)
     atgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATg                                                                                                 <  1:3317828/100‑1 (MQ=255)
      tgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTATTAACAACCCTGGCCCGGTACACATg                                                                                                 >  1:3444092/1‑99 (MQ=255)
       gatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGtt                                                                                              <  2:3025807/100‑1 (MQ=255)
         tgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGtgtac                                                                                            >  1:2343169/1‑96 (MQ=255)
          ggAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTAcc                                                                                           <  2:2952897/100‑1 (MQ=255)
           gAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCg                                                                                          >  2:3786801/1‑100 (MQ=255)
            aaaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCgg                                                                                         <  2:1591241/100‑1 (MQ=255)
             aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCggg                                                                                        <  1:704797/100‑1 (MQ=255)
             aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCggg                                                                                        <  2:3612727/100‑1 (MQ=255)
              aaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGc                                                                                       >  1:1135523/1‑100 (MQ=255)
              aaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGc                                                                                       <  1:1937410/100‑1 (MQ=255)
                aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCt                                                                                     <  2:767702/100‑1 (MQ=255)
                aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGcca                                                                                     <  2:2943085/100‑2 (MQ=39)
                 ngCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTa                                                                                    >  2:2289362/2‑100 (MQ=255)
                 ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                    <  2:3372376/100‑3 (MQ=39)
                 ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                    <  2:2381937/100‑3 (MQ=39)
                  gCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTAc                                                                                   >  2:440253/1‑100 (MQ=255)
                   cTACCCCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGCCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACa                                                                                  <  1:2046039/100‑1 (MQ=255)
                   cTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACa                                                                                  <  2:3077839/100‑1 (MQ=255)
                   cTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACa                                                                                  <  2:3980771/100‑1 (MQ=255)
                      cacCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccagggttg                                                                               <  1:1737079/100‑8 (MQ=37)
                        cccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGAc                                                                             >  2:1847588/1‑100 (MQ=255)
                        cccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGAc                                                                             >  2:1853168/1‑100 (MQ=255)
                        cccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGAc                                                                             >  1:3094284/1‑100 (MQ=255)
                           gTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGc                                                                          <  2:560565/100‑1 (MQ=255)
                               aCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAAc                                                                      <  2:1447501/100‑1 (MQ=255)
                                cAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAAcc                                                                     <  1:1578953/100‑1 (MQ=255)
                                  ggTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGt                                                                   >  2:2228164/1‑100 (MQ=255)
                                       gTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGcc                                                              <  2:89027/100‑1 (MQ=255)
                                          tACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTga                                                           >  1:882968/1‑100 (MQ=255)
                                             tgtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGaa                                                        >  2:3563532/1‑100 (MQ=255)
                                              gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg                                                       >  1:1616575/1‑100 (MQ=255)
                                              gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg                                                       >  2:1015318/1‑100 (MQ=255)
                                                gCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCg                                                     <  1:1132972/100‑1 (MQ=255)
                                                gCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCg                                                     >  2:2690738/1‑100 (MQ=255)
                                                gCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCg                                                     >  2:2835968/1‑100 (MQ=255)
                                                gCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCg                                                     >  2:1954585/1‑100 (MQ=255)
                                                gCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCg                                                     >  1:72669/1‑100 (MQ=255)
                                                gCGAATGGTATTTCCGCGTACGTGAATCGCCTTAACAACCCTGGCACGGAACACATCTTACCGGGCATAAAACGACCGCGCACCGCGCGCCTgcgc                                                         >  1:3964538/1‑93 (MQ=255)
                                                 cGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCgg                                                    >  2:3800393/1‑100 (MQ=255)
                                                 cGAAGGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCgg                                                    <  1:2555908/100‑1 (MQ=255)
                                                  gAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGc                                                   <  2:2031231/100‑1 (MQ=255)
                                                  gAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGc                                                   <  1:1509422/100‑1 (MQ=255)
                                                  gAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGACCTGAGAAGCGGc                                                   <  1:1387129/100‑1 (MQ=255)
                                                   aaTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCa                                                  <  1:2397993/100‑1 (MQ=255)
                                                     tGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAac                                                <  2:75162/100‑1 (MQ=255)
                                                     tGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAac                                                >  1:2668174/1‑100 (MQ=255)
                                                         aTTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg                                            >  2:1252845/1‑100 (MQ=255)
                                                          tttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCTACACAgg                                           <  2:2238935/100‑1 (MQ=255)
                                                          tttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAgg                                           <  2:3453188/100‑1 (MQ=255)
                                                          tttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAgg                                           <  1:4086823/100‑1 (MQ=255)
                                                                cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGc                                     >  1:4092985/1‑100 (MQ=255)
                                                                  tacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCAt                                   >  1:1282942/1‑100 (MQ=255)
                                                                    cgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATAc                                 <  1:1694050/100‑1 (MQ=255)
                                                                      taAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAg                               <  1:1768471/100‑1 (MQ=255)
                                                                       aaaTCGTCTTAACAACCCTGGACCGGTACAAATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCATGAGAAGCGGCAACACAGGCGTAGCATACAGt                              >  1:2482229/1‑100 (MQ=255)
                                                                        aaTCGTCTTACCAACCCTGGCCCGGTAGCCATGTTACCGGGCCTACACCGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGtt                             <  2:2326829/100‑1 (MQ=255)
                                                                         aTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTg                            <  2:420746/100‑1 (MQ=255)
                                                                         aTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTg                            <  2:3558359/100‑1 (MQ=255)
                                                                         aTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTg                            <  1:3910405/100‑1 (MQ=255)
                                                                          tCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTgg                           <  1:2447390/100‑1 (MQ=255)
                                                                           cGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGCACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                          <  1:3470056/100‑1 (MQ=255)
                                                                           cGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                          <  1:1539583/100‑1 (MQ=255)
                                                                           cGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                          <  2:2791694/100‑1 (MQ=255)
                                                                            gTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                          >  2:1703198/1‑99 (MQ=255)
                                                                            gTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa                         <  2:3425154/100‑1 (MQ=255)
                                                                              cTTCACAACCCTGGCCCGGTACACATCTGACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACTGGCGTAGCATACAGTTGGCaaa                       <  1:2477508/100‑1 (MQ=255)
                                                                              cTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCaaa                       >  2:3374378/1‑100 (MQ=255)
                                                                                tAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTCGCATACAGTTGGCAAATg                     >  1:1295657/1‑100 (MQ=255)
                                                                                tAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATg                     <  2:3752791/100‑1 (MQ=255)
                                                                                 aacaccCCTGGCCCGGTCCACATGTTCCCGGTCCTCCAACCACAGCGAACCGTGGGCCTGAGAAGCGGTAACACAGGCGTAGCATACAGTTGGCaat                       <  1:1872443/97‑2 (MQ=255)
                                                                                 aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt                    >  1:4204777/1‑100 (MQ=255)
                                                                                 aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt                    >  2:1985474/1‑100 (MQ=255)
                                                                                  acaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgta                   >  1:2197320/1‑100 (MQ=255)
                                                                                   caacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtag                  >  2:3226581/1‑100 (MQ=255)
                                                                                   caacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtag                  <  1:627531/100‑1 (MQ=255)
                                                                                    aacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACATTTGCCAACTgtatg                 <  2:1663489/100‑3 (MQ=255)
                                                                                    aacCCTGACCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAAAgta                   >  1:3583284/1‑98 (MQ=255)
                                                                                         tGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg            <  2:663593/100‑1 (MQ=255)
                                                                                         tGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg            <  2:2330820/100‑1 (MQ=255)
                                                                                          ggCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggg           >  2:3267112/1‑100 (MQ=255)
                                                                                           gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg          >  1:3390291/1‑100 (MQ=255)
                                                                                           gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg          <  2:3076839/100‑1 (MQ=255)
                                                                                           gCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg          >  2:1558114/1‑100 (MQ=255)
                                                                                           gCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg          >  2:2719329/1‑100 (MQ=255)
                                                                                           gCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggg           >  1:3878722/1‑99 (MQ=255)
                                                                                            cccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggggg         >  1:1395694/1‑100 (MQ=255)
                                                                                            cccGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGAATGAGAAGCGGCAACACAGGCGAAGCATAAAGTTGGCAAAGGTAGTACgcggg          >  1:2079220/1‑99 (MQ=255)
                                                                                             ccGGTACACATGTGACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc        <  1:2685178/100‑1 (MQ=255)
                                                                                              cGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCa       <  2:3335550/100‑1 (MQ=255)
                                                                                                gTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCata     <  2:1282946/100‑1 (MQ=255)
                                                                                                gTACACATGTTACCGGGCCTACAACGACAGCGAACCGTCGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCata     <  1:820708/100‑1 (MQ=255)
                                                                                                  acacATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATg   <  2:2526220/100‑1 (MQ=255)
                                                                                                  acacATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATg   <  2:2267430/100‑1 (MQ=255)
                                                                                                   cacaTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGa  <  2:2425660/100‑1 (MQ=255)
                                                                                                   cacaTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGa  >  1:4200226/1‑100 (MQ=255)
                                                                                                   |                                                                                                    
ACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGA  >  NC_000913/1868760‑1868958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: