BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,989,323	(A)_8→7	100%	coding (20/483 nt)	yqeJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,989,316	0	A	.	53.3%	250.2 / inf	154	coding (13/483 nt)	yqeJ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (34/36); new base . (46/36); total (80/74)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

CGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCA  >  NC_000913/2989217‑2989415

cGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATta‑ta                                                                                                     <  1:1688632/100‑1 (MQ=255)

    gatgatTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGCTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑Taaaaa                                                                                                 >  2:311377/1‑100 (MQ=255)

        atTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑Taaaaaaact                                                                                             <  2:1362988/100‑3 (MQ=255)

        atTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAAc                                                                                             <  2:718006/100‑1 (MQ=255)

         ttGTTATGGCATGAAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑Taaaaaaactt                                                                                            <  2:3218998/100‑4 (MQ=255)

          tGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑Taaaaaaactta                                                                                           <  2:1194925/100‑5 (MQ=255)

          tGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑Taaaaaaactta                                                                                           <  1:2762241/100‑5 (MQ=255)

           gTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACttat                                                                                         >  2:2092743/1‑100 (MQ=255)

            ttATGGCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACttat                                                                                         >  2:3039473/1‑100 (MQ=255)

             tATGGAATTCAATAAAAACTATAACCAACATGACGTGG‑CCAACTGTTTAACCCTGTATTACAGTGAGGTTACTCGTGATTGATTA‑T‑AAAAAAACttatt                                                                                        >  1:643060/1‑99 (MQ=255)

              aTGGCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTtattat                                                                                      >  1:936085/1‑100 (MQ=255)

                ggCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTAttattt                                                                                    >  2:3763947/1‑100 (MQ=255)

                 gCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTAttattta                                                                                   >  2:515165/1‑100 (MQ=255)

                  cATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTAttattta                                                                                   >  1:3760062/1‑100 (MQ=255)

                    tGCGATAAAAACTATAACCAACATG‑AGTGGTCATTCTGTTTAACCTTCTCTTACCGTGGGGTTAATCGGGATTGGTTA‑T‑AAAAAAACTTAttatttat                                                                                  <  1:2466170/98‑1 (MQ=255)

                      cAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTa                                                                              <  2:832953/100‑1 (MQ=255)

                      cAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTAACGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTAtttt                                                                               >  1:1349436/1‑100 (MQ=255)

                       aaTTAAAATTATAACCTACATGAGATGG‑CAAACTGTTTAACTCTGTCTCACCGTAAGGTTAATCGTTAATAATTA‑TAAAAAAAACTTATtatttatt                                                                                 <  1:856113/97‑1 (MQ=255)

                       aaTAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTa                                                                              >  1:3778632/1‑100 (MQ=255)

                         tAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGt                                                                            >  1:4069626/1‑100 (MQ=255)

                         tAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGtt                                                                           >  1:3926350/1‑100 (MQ=255)

                          aaaaaCTATAACCAACATGGAGTGG‑CAAACTGTTTAACCATGTCTTACCGTGATGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGtt                                                                           <  1:495163/99‑1 (MQ=255)

                          aaaaaCTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGtt                                                                           <  2:1253256/100‑1 (MQ=255)

                          aaaaaCTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGttt                                                                          >  2:2028659/1‑100 (MQ=255)

                          aaaaaCTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGttt                                                                          <  2:2353204/100‑1 (MQ=255)

                            aaaCTATAACCAACATGAAGTGG‑CACACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGttttt                                                                        <  2:2324630/100‑1 (MQ=255)

                            aaaCTATAACCAACATGAAGTCG‑CAAACAATTTAACACTGTCTTACCGTGATGTTAATAAAAAAAAAAAA‑‑AAAAAAAACTTATTATTTATTTTAGttttt                                                                        <  1:2517552/100‑1 (MQ=255)

                             aaCTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGttttt                                                                        >  1:3132442/1‑100 (MQ=255)

                              acgataACCAACAAGAAGTAA‑CAAAATGTATAACACTGTATTATCGTGATTTTAATCGTAAAAAAAAA‑‑AAAAAAAACTTATTATTTATTTTAGTTTTTAt                                                                      <  1:1640066/97‑1 (MQ=255)

                              aCTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTAt                                                                      >  1:1718822/1‑100 (MQ=255)

                               cTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATc                                                                     >  2:883234/1‑100 (MQ=255)

                                tataACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TTAAAAAAACTTATTATTTATTTTAGTTTTTATc                                                                     <  2:4060646/100‑1 (MQ=255)

                                  taACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAg                                                                   <  2:1416360/100‑1 (MQ=255)

                                   aaCCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTg                                                                 <  1:4003233/100‑1 (MQ=255)

                                    aCCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTg                                                                 <  2:2541913/100‑1 (MQ=255)

                                    aCCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTgg                                                                >  2:2386568/1‑100 (MQ=255)

                                    aCCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTgg                                                                <  2:1057906/100‑1 (MQ=255)

                                     ccAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTggg                                                               >  1:1013138/1‑100 (MQ=255)

                                      cAACATTAAGGGG‑CGAGCTGTTTAACCCTGTCGTAAAGTTAAGTTAATCGTAATTGATTA‑‑GAAAAAAACTTAGTATTTTTTTTAGTATTTATCAGtt                                                                 <  1:751365/97‑2 (MQ=255)

                                      cAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGt                                                              <  2:1457521/100‑1 (MQ=255)

                                       aaCATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGt                                                              >  1:603004/1‑100 (MQ=255)

                                        aCATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGtt                                                             <  1:1327891/100‑1 (MQ=255)

                                        aCATGAAGTGA‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATAGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGttt                                                            >  1:202601/1‑100 (MQ=255)

                                          aTGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTAt                                                          >  2:878582/1‑100 (MQ=255)

                                           tGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTAt                                                          >  2:2992695/1‑100 (MQ=255)

                                           tGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTAt                                                          >  1:2973766/1‑100 (MQ=255)

                                             aaGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATtc                                                        <  2:1364148/100‑1 (MQ=255)

                                                tGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTctct                                                     >  2:1609043/1‑100 (MQ=255)

                                                tGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCtt                                                    <  2:598559/100‑1 (MQ=255)

                                                 gg‑CAAACTGTTTAACCCTGTCTTACTGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCtttt                                                    >  1:2658621/1‑100 (MQ=255)

                                                 gg‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTc                                                   <  2:2634366/100‑1 (MQ=255)

                                                 gg‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTc                                                   <  1:3272803/100‑1 (MQ=255)

                                                  g‑CAAACTGTTTAACCTTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTc                                                   <  2:4213336/100‑1 (MQ=255)

                                                  g‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTc                                                   <  1:3207274/100‑1 (MQ=255)

                                                  g‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCa                                                  >  1:2555683/1‑100 (MQ=255)

                                                    cAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCa                                                  <  2:2307224/100‑1 (MQ=255)

                                                    cAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCa                                                  >  1:3018175/1‑100 (MQ=255)

                                                    cAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCAc                                                 <  2:1929727/100‑1 (MQ=255)

                                                     aaaCTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACt                                                <  2:1718827/100‑1 (MQ=255)

                                                     aaaCTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACt                                                >  1:3359273/1‑100 (MQ=255)

                                                     aaaCTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATCTTAGTTTTTATCAGTGGGTTTATTCTCTTCACt                                                >  2:780714/1‑100 (MQ=255)

                                                       aCTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTg                                               <  2:334938/100‑1 (MQ=255)

                                                         tGTTTAACCATGTCTTACCGTGAGGTTAATCGTGATTGAAAA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGt                                              <  1:1725036/99‑1 (MQ=255)

                                                          gTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAGAAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGt                                              >  1:2321600/1‑99 (MQ=255)

                                                          gTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCt                                            >  2:983148/1‑100 (MQ=255)

                                                          gTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTa                                           <  1:722300/100‑1 (MQ=255)

                                                            ttAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTAtt                                         >  1:1800007/1‑100 (MQ=255)

                                                              aaCCCTTTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTc                                        <  1:2928244/100‑1 (MQ=255)

                                                              aaCCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCt                                       <  1:1195209/100‑1 (MQ=255)

                                                               aCCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCtt                                      <  1:2066132/100‑1 (MQ=255)

                                                                cccTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCtt                                      >  1:3829688/1‑100 (MQ=255)

                                                                  cTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTac                                    <  1:2223706/100‑1 (MQ=255)

                                                                   tGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTaca                                   >  1:1382393/1‑100 (MQ=255)

                                                                      cTTACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCACTTCACTGTCTATTCTTACACTGc                               >  1:3390344/1‑100 (MQ=255)

                                                                       ttACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGc                               <  1:1948113/100‑1 (MQ=255)

                                                                       ttACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCa                              <  1:502842/100‑1 (MQ=255)

                                                                       ttACCGTGAGGTTAATCGTGATAAATTA‑‑AAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGc                               <  1:3600539/99‑1 (MQ=255)

                                                                        tACTGTGAGGTTAATCGTGATTAAAAA‑AAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTg                                <  1:1593234/98‑1 (MQ=255)

                                                                        tACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAg                             <  1:3637997/100‑1 (MQ=255)

                                                                        tACCGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAg                             <  2:4114558/100‑1 (MQ=255)

                                                                         aCCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAg                             >  2:2810382/1‑100 (MQ=255)

                                                                          ccGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGaa                           >  1:1780557/1‑100 (MQ=255)

                                                                          ccGTGAGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGaa                           >  1:1750743/1‑100 (MQ=255)

                                                                               aGGTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTTTCTATTCTTACACTGCAGAAAAAAt                      >  1:297447/1‑100 (MQ=255)

                                                                                ggTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTATTACACTGTAGAAAAAAt                      >  2:94406/1‑100 (MQ=255)

                                                                                ggTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTAAGTTCTTATCAACGAGGTTATTAACTTAACTGTGTATTTTTACACTGCAGgaa                          >  1:932850/1‑96 (MQ=255)

                                                                                ggTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATg                     <  2:4082345/100‑1 (MQ=255)

                                                                                ggTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATg                     <  2:274656/100‑1 (MQ=255)

                                                                                 gTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCTGTGGGTTTATTCTCTTCACTGTTTATTCTTACACTGCAGAAAAAAt                      >  1:3570790/1‑98 (MQ=255)

                                                                                 gTTAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATg                     >  1:2798994/1‑99 (MQ=255)

                                                                                   tAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTAACTGTCTATTCTTACACTGCAGAAAAAATGat                   >  2:2970932/1‑100 (MQ=255)

                                                                                   tAATCGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGata                  <  1:129696/100‑1 (MQ=255)

                                                                                    aaTCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGata                  <  2:3454920/100‑1 (MQ=255)

                                                                                       cGTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGAtatata              <  2:1432061/100‑1 (MQ=255)

                                                                                        gTGATTGATTA‑T‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATaa             >  2:1733881/1‑100 (MQ=255)

                                                                                             tgattA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGaa         <  2:3132454/100‑1 (MQ=255)

                                                                                              gattA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGaaa        >  2:1123317/1‑100 (MQ=255)

                                                                                               attA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTTTGTTTATTCTCTTCACTTTATATTCTTACACTGCAGAAAAAATGATATATAACGAAAc       <  1:2103973/100‑1 (MQ=255)

                                                                                                ataaaaaaaaaaaCTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGaaa        <  1:3141897/94‑1 (MQ=255)

                                                                                                    taAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGc   <  1:2824792/100‑1 (MQ=255)

CGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGG‑CAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTA‑TAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCA  >  NC_000913/2989217‑2989415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`