Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,360,916 (A)6→5 100% intergenic (+300/‑260) gltD → / → gltF glutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,360,9110A.50.3% 319.2 / inf 173intergenic (+295/‑265)gltD/gltFglutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (42/44);  new base . (44/43);  total (86/87)
Fisher's exact test for biased strand distribution p-value = 8.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 188 total aligned reads displayed.

ATTACTTGTTAACACCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCA  >  NC_000913/3360812‑3361009
                                                                                                   |                                                                                                  
aTTACTTGTTAACACCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAAtata                                                                                                    >  2:1463664/1‑100 (MQ=255)
    cTTGTTAACACCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATaaaaa                                                                                                >  1:110734/1‑100 (MQ=255)
    cTTGTTAACACCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATaaaaa                                                                                                <  1:1386919/100‑1 (MQ=255)
    cTTGTTAACACCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATaaaaa                                                                                                >  2:1347192/1‑100 (MQ=255)
    cTTGTTAACACCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATaaaaa                                                                                                >  1:3432454/1‑100 (MQ=255)
      tGTTAACACTTCTGACAATACCGATTCTACAAGCTATTCAGTCTTTTCATTCAATGTAAATTGTCTCGATATTAATATACAAAATATGAATATaaaaaa                                                                                               >  1:2257173/1‑99 (MQ=255)
      tGTTAACACCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAAc                                                                                              >  1:3569937/1‑100 (MQ=255)
      tGTTAACACCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAAc                                                                                              <  1:3517097/100‑1 (MQ=255)
      tGTTAACACCTCTGACAATACCGATTCTGCAAGCTATTAAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAAc                                                                                              <  1:1945792/100‑1 (MQ=255)
       gTTAACACCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATaaaaac                                                                                               <  2:1655020/98‑2 (MQ=255)
        ttAACACCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCa                                                                                            <  1:1074315/100‑1 (MQ=255)
         tAACACCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCaa                                                                                           >  2:3515027/1‑100 (MQ=255)
           acacCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAata                                                                                         >  1:398551/1‑100 (MQ=255)
            cacCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAatata                                                                                       >  1:526798/1‑100 (MQ=255)
             acCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAatata                                                                                       <  1:3175597/100‑1 (MQ=255)
                  tGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATc                                                                                  <  2:166783/100‑1 (MQ=255)
                  tGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATcc                                                                                 <  1:573456/100‑1 (MQ=255)
                   gACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATcc                                                                                 <  1:773654/100‑1 (MQ=255)
                      aaTACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTa                                                                              >  1:2977066/1‑100 (MQ=255)
                       aTACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAAt                                                                            <  2:2830949/100‑1 (MQ=255)
                          ccGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTAt                                                                         >  1:3431535/1‑100 (MQ=255)
                           cGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTAt                                                                         >  2:600514/1‑100 (MQ=255)
                           cGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTAt                                                                         >  1:1557151/1‑100 (MQ=255)
                            gATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATc                                                                        >  1:2423123/1‑100 (MQ=255)
                            gATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATc                                                                        <  2:495462/100‑1 (MQ=255)
                             aTTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCtg                                                                      <  2:4260183/100‑2 (MQ=255)
                              ttCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTGATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTa                                                                          <  1:2367703/96‑1 (MQ=255)
                              ttCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCtat                                                                     <  2:339954/100‑1 (MQ=255)
                                 taaaaacTATTAAATCTTTTAATTCCATATAAATTCTCTCGATATTAATATATAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCtat                                                                     <  1:3153202/92‑1 (MQ=255)
                                 tGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCtatata                                                                  >  2:3759812/1‑100 (MQ=255)
                                   cAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATAtt                                                                >  2:2304761/1‑100 (MQ=255)
                                    aaGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATAtt                                                                <  1:331414/100‑1 (MQ=255)
                                    aaGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATAttt                                                               >  1:3672617/1‑100 (MQ=255)
                                      gCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATATACACCAATATATTTTCCTTAATTATCCATATAttt                                                               >  1:1903061/1‑98 (MQ=255)
                                        tATTCAGTCTCTTCATTCCATGTAAATTGTCACGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTcc                                                            <  2:2987818/100‑1 (MQ=255)
                                         aTTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCt                                                           >  1:1849637/1‑100 (MQ=255)
                                          ttCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTAt                                                         >  2:2824582/1‑100 (MQ=255)
                                           tCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTAtt                                                        <  2:3744810/100‑1 (MQ=255)
                                           tCAGTCTTTTCATTCCATGTAAATTGTATAGATATTAATATACAAAATATGAATATAAAAAAACAATATATTATCCTTAATTATTTATATATTTTcat                                                           >  1:3778692/1‑96 (MQ=255)
                                             agacTTTTCATACCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTAtttt                                                      <  1:1062075/97‑1 (MQ=255)
                                             aGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTAttt                                                       >  2:3186330/1‑100 (MQ=255)
                                              gTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTAtttt                                                      >  2:3235828/1‑100 (MQ=255)
                                              gTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTAttttt                                                     >  2:1856054/1‑100 (MQ=255)
                                              gTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTAtttt                                                      <  1:1928039/99‑1 (MQ=255)
                                                cTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCa                                                   >  2:107490/1‑100 (MQ=255)
                                                 ttttCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTAttttttt                                                   >  1:2002523/1‑98 (MQ=255)
                                                 ttttCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCa                                                   <  1:1642781/99‑1 (MQ=255)
                                                    tCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAgcgc                                               <  1:1758910/100‑1 (MQ=255)
                                                      aTTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCa                                              >  1:252206/1‑100 (MQ=255)
                                                      aTTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTAACTATATATTTTCCTATTTTTAAgcg                                                >  1:1980957/1‑98 (MQ=255)
                                                      aTTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCATTAATTATCTATATATTTTCCTATTTTTCAGCGCa                                              >  1:254720/1‑100 (MQ=255)
                                                         ccATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAAc                                           <  2:2955101/100‑1 (MQ=255)
                                                         ccATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACa                                          <  1:2793728/100‑1 (MQ=255)
                                                          cATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACa                                          >  2:1337688/1‑100 (MQ=255)
                                                          cATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACa                                          <  2:1738291/100‑1 (MQ=255)
                                                          cATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACAt                                         >  1:4252670/1‑100 (MQ=255)
                                                            tGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTc                                       >  1:2212472/1‑100 (MQ=255)
                                                            tGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTc                                       >  1:3939740/1‑100 (MQ=255)
                                                             gTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCa                                      <  1:655543/100‑1 (MQ=255)
                                                               aaaTTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCa                                      <  2:538509/99‑1 (MQ=255)
                                                               aaaTTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCAt                                     >  2:3672969/1‑100 (MQ=255)
                                                               aaaTTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATg                                    >  2:3798923/1‑100 (MQ=255)
                                                               aaaTTGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATg                                    <  1:2133114/100‑1 (MQ=255)
                                                                  ttGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTaa                                 <  2:404344/100‑1 (MQ=255)
                                                                  ttGTCTCGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTaa                                 <  1:2304755/100‑1 (MQ=255)
                                                                     tctcGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAAt                                >  1:839081/1‑99 (MQ=255)
                                                                      ctcGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGaa                             >  2:2195160/1‑100 (MQ=255)
                                                                      ctcGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGaa                             >  1:3357909/1‑100 (MQ=255)
                                                                      ctcGATATTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGa                              >  1:2070723/1‑99 (MQ=255)
                                                                        cGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGaat                            <  2:4132734/100‑1 (MQ=255)
                                                                        cGATAATAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTCTTTTTCAGCGCAAACATTCATGTAATGaat                            <  2:1868348/100‑1 (MQ=255)
                                                                          atatTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATg                                    <  1:978212/89‑1 (MQ=255)
                                                                          atatTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGaataat                         <  2:3131176/100‑1 (MQ=255)
                                                                            atTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTa                       >  2:1173645/1‑100 (MQ=255)
                                                                            atTAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTa                       <  2:3567996/100‑1 (MQ=255)
                                                                             ttAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTa                       >  2:676071/1‑100 (MQ=255)
                                                                             ttAATATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTAt                      >  2:2754353/1‑100 (MQ=255)
                                                                               aaTATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAGACATTCATGTAATGAATAATTATc                     <  1:1461629/100‑1 (MQ=255)
                                                                               aaTATACAAAATATGAATATAAAAAACCAATATATTAGCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATTACATGaatgtttgcg                     <  1:4051356/100‑8 (MQ=255)
                                                                               aaTATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATca                    >  2:389765/1‑100 (MQ=255)
                                                                               aaTATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATca                    <  2:2225273/100‑1 (MQ=255)
                                                                               aaTATACAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATca                    >  2:759421/1‑100 (MQ=255)
                                                                                 tataCAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATcac                   <  2:1078116/100‑1 (MQ=255)
                                                                                  gatctaaaTATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATcacc                  <  2:2633708/95‑1 (MQ=255)
                                                                                  ataCAAAATATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATTAATAATTATcacc                  <  2:2106647/99‑1 (MQ=255)
                                                                                   taCAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATcacca                 >  2:1524156/1‑100 (MQ=255)
                                                                                     cAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTTATCTTAAACATTCATGTAATGAATAATTATCACcaca               >  1:3039212/1‑100 (MQ=255)
                                                                                     cAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACcaca               >  2:484111/1‑100 (MQ=255)
                                                                                     cAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATAGATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACcaca               >  2:1917615/1‑100 (MQ=255)
                                                                                     cAAAATATGAATATAAAAACCAATATATTATCGTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACaa              >  2:3330578/1‑100 (MQ=255)
                                                                                       aaaTATGAATATAAAAAACCAATATATTATCCTTAATTGTCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATcaccagatc             >  1:2633699/1‑96 (MQ=255)
                                                                                        aaTATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAacc           <  1:4092956/100‑1 (MQ=255)
                                                                                        aaTATGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAacc           >  2:3701417/1‑100 (MQ=255)
                                                                                          tatGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAacca          <  2:1559192/100‑1 (MQ=255)
                                                                                          tatGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAaccac         <  2:3650966/100‑1 (MQ=255)
                                                                                           atGAATATAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAaccacc        <  2:902757/100‑1 (MQ=255)
                                                                                            tGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAaccacc        <  1:1833568/100‑1 (MQ=255)
                                                                                              aaTATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTc      >  2:2386067/1‑100 (MQ=255)
                                                                                                tataAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCAc    >  2:3102973/1‑100 (MQ=255)
                                                                                                  taAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCa  >  1:2751856/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
ATTACTTGTTAACACCTCTGACAATACCGATTCTGCAAGCTATTCAGTCTTTTCATTCCATGTAAATTGTCTCGATATTAATATACAAAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCA  >  NC_000913/3360812‑3361009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: