Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,520,500 Δ1 bp 16.0% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5000A.16.0% 573.2 / 107.9 200intergenic (‑176/+171)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base A (82/86);  new base . (18/14);  total (100/100)
Fisher's exact test for biased strand distribution p-value = 5.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

Only 100 of 208 total aligned reads displayed.

TGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGC  >  NC_000913/4520401‑4520599
                                                                                                   |                                                                                                    
tGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTATAAAGCCGgaga                                                                                                      >  1:2062054/1‑100 (MQ=255)
 ggCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGAc                                                                                                     <  2:1887206/100‑1 (MQ=255)
   cATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAt                                                                                                   >  2:85292/1‑100 (MQ=255)
   cATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAt                                                                                                   >  1:617544/1‑100 (MQ=255)
    aTGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATg                                                                                                  <  1:1879751/100‑1 (MQ=255)
      gATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCt                                                                                               >  2:3498331/1‑100 (MQ=255)
        ttttATACAGATATTTTATTCTTTTACATGAAATAACCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTcc                                                                                             <  2:1191976/100‑1 (MQ=255)
           tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGc                                                                                          >  1:1365322/1‑100 (MQ=255)
           tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGc                                                                                          <  2:3907422/100‑1 (MQ=255)
            ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCt                                                                                         <  1:3320703/100‑1 (MQ=255)
             taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCtt                                                                                        <  2:567090/100‑1 (MQ=38)
             taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCtt                                                                                        <  2:1935271/100‑1 (MQ=38)
              aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCttt                                                                                       >  2:3984931/1‑100 (MQ=255)
              aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCttt                                                                                       <  2:831811/100‑1 (MQ=255)
              aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCttt                                                                                       <  1:3474015/100‑1 (MQ=38)
              aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCAATACTGTAAAGCCGGAGCATGTCTCCGGCttt                                                                                       <  1:3191638/100‑1 (MQ=38)
               cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGGGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                      <  1:683985/100‑1 (MQ=255)
               cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                      >  1:2078443/1‑100 (MQ=255)
               cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                      >  1:737139/1‑100 (MQ=255)
                aGATATTTTATTCTTTTACATGACATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTGCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                      <  1:2712336/99‑1 (MQ=255)
                aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTAc                                                                                     <  2:1752692/100‑1 (MQ=255)
                aGATATTTTATTCTATTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTAc                                                                                     <  1:2908898/100‑1 (MQ=255)
                 gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCTATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATACTCCGGCTTTACa                                                                                    >  1:1000322/1‑100 (MQ=255)
                  atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCTATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAg                                                                                   <  1:788404/100‑1 (MQ=37)
                    atTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCTGGCTTTACAGTa                                                                                 <  2:2813794/100‑1 (MQ=255)
                     ttttATTCTTTTACATGAAATAAACCAGCAAACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCTGGCTTTACAGTAt                                                                                >  1:2809342/1‑100 (MQ=255)
                        tATTCGTTTACATGACATAAACCATCATACCATGTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTAc                                                                             <  1:3924553/100‑1 (MQ=39)
                          ttCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTg                                                                           <  2:3334990/100‑1 (MQ=255)
                            cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgag                                                                         >  1:4160870/1‑100 (MQ=255)
                            cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgag                                                                         >  1:3249561/1‑100 (MQ=255)
                                  naTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                   >  1:2089937/2‑100 (MQ=255)
                                  cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                   >  2:1861375/1‑100 (MQ=255)
                                  cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                   >  1:4052353/1‑100 (MQ=255)
                                  cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                   >  1:2142451/1‑100 (MQ=255)
                                  cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                   >  1:2579690/1‑100 (MQ=255)
                                     gAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAccc                                                                >  2:4269183/1‑100 (MQ=255)
                                       aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCAt                                                              >  2:1618851/1‑100 (MQ=255)
                                         tAAACCAGCATACCATTTGAGCGATCGCCCGCTTTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATc                                                             <  1:1628520/99‑1 (MQ=255)
                                          aaaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                           >  2:1892727/1‑100 (MQ=255)
                                           aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                          <  1:1274455/100‑1 (MQ=255)
                                           aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                          <  2:2389444/100‑1 (MQ=255)
                                           aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                          >  2:3283160/1‑100 (MQ=255)
                                             ccAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                        <  2:1634311/100‑1 (MQ=255)
                                               aGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATa                                                      <  2:2081471/100‑1 (MQ=255)
                                                gCATACCATTTGAGCGATCGCTCGCTGTTTCCGCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCAt                                                       >  1:1731611/1‑98 (MQ=255)
                                                  aTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                   <  1:1760567/100‑1 (MQ=255)
                                                   tACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                  >  2:3846010/1‑100 (MQ=255)
                                                    aCCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                   <  1:715520/98‑1 (MQ=255)
                                                     ccATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                <  2:1106740/100‑1 (MQ=255)
                                                     ccATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                >  2:2507580/1‑100 (MQ=255)
                                                       aTTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAgg                                              <  2:2307736/100‑1 (MQ=255)
                                                         ttGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGtt                                            >  1:2689163/1‑100 (MQ=255)
                                                          tctgCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTc                                           <  1:2490048/97‑1 (MQ=255)
                                                          tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATTCCCCCATCAAGCATAATAGGAGGtt                                            >  2:3355051/1‑99 (MQ=38)
                                                           gAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATATACCCATCAAGCATAATCGAAGGTTCa                                          <  2:2603425/100‑1 (MQ=255)
                                                             gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                        >  1:748173/1‑100 (MQ=255)
                                                             gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                        >  2:3360445/1‑100 (MQ=255)
                                                               ggtCGCCCGCTGTGCACACATACGGTAAAGCCAGAGACATGCGACGGCTTTACAGACTTACTCAGAATAGACCCATCCAGCATAATCGAAGGTCCAat                                        >  1:2174326/3‑98 (MQ=255)
                                                               gagcGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCa                                          <  1:1240412/93‑1 (MQ=255)
                                                                  cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                   <  2:4160892/100‑1 (MQ=255)
                                                                  cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAGGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                   <  1:342037/100‑1 (MQ=255)
                                                                          gTTCCCCCATACTGTAAGGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAAGACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                           >  1:194257/1‑100 (MQ=255)
                                                                          gTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTCTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                           <  1:3442669/100‑1 (MQ=255)
                                                                           ttCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGTTTCAATATCGCCCCTTTTTg                          <  1:2603506/100‑1 (MQ=255)
                                                                           ttCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTg                          <  1:3586676/100‑1 (MQ=255)
                                                                           ttCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTg                          <  1:3726704/100‑1 (MQ=255)
                                                                           ttCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTg                          <  2:2166542/100‑1 (MQ=255)
                                                                            tCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                         >  2:3185901/1‑100 (MQ=255)
                                                                             cccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                        >  2:2228182/1‑100 (MQ=255)
                                                                              ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                       >  1:1787042/1‑100 (MQ=255)
                                                                               cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCACGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                      >  1:3022573/1‑100 (MQ=255)
                                                                               cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                      <  1:578943/100‑1 (MQ=255)
                                                                               cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                      <  2:3296825/100‑1 (MQ=255)
                                                                                    aCTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTGTTTGATCGCCAGc                 <  2:2901342/100‑1 (MQ=255)
                                                                                     cTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCTTCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                  <  2:3643977/98‑1 (MQ=255)
                                                                                     cTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATACTCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                 >  1:3817463/1‑99 (MQ=255)
                                                                                     cTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                 >  1:846546/1‑99 (MQ=38)
                                                                                     cTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                >  2:708420/1‑100 (MQ=38)
                                                                                     cTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                >  2:1226003/1‑100 (MQ=38)
                                                                                      tGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGATCAATATCGCCCCTTTTTGATCGCCAGCGa               >  2:2356903/1‑100 (MQ=37)
                                                                                      tGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAAAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATAGCCAGCGa               >  1:2744510/1‑100 (MQ=37)
                                                                                       gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCCATATCGCCCCTTTTTGATCCCCCGc                 >  1:2403821/1‑97 (MQ=255)
                                                                                       gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt              >  1:906494/1‑100 (MQ=255)
                                                                                       gTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt              >  1:1207936/1‑100 (MQ=38)
                                                                                        tAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt             <  2:617546/100‑1 (MQ=255)
                                                                                        tAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCGATATCGCCCCTTTTTGATCGCCAGCGAtt             >  1:1528068/1‑100 (MQ=38)
                                                                                        tAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt              >  1:2946064/1‑99 (MQ=38)
                                                                                         aaaGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc            >  2:324339/1‑100 (MQ=38)
                                                                                         aaaGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAAACGAAGGTTCAATATCGACCCTTTTTGATCGCCAGCGACTc            >  2:1627107/1‑100 (MQ=37)
                                                                                          aaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg           <  2:1048786/100‑1 (MQ=255)
                                                                                          aaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg           >  2:1560435/1‑100 (MQ=255)
                                                                                           aGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGt          >  1:1227775/1‑100 (MQ=38)
                                                                                            gCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTc         >  2:1379197/1‑100 (MQ=255)
                                                                                            gCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTc         <  1:1084353/100‑1 (MQ=255)
                                                                                              cGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCAc       <  2:1507283/100‑1 (MQ=255)
                                                                                              cGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCAc       <  2:3255175/100‑1 (MQ=255)
                                                                                               ggAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCAcc      >  2:3434533/1‑100 (MQ=255)
                                                                                                gagaCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCg     <  1:486399/100‑1 (MQ=255)
                                                                                                 ataCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCg     <  1:512593/97‑1 (MQ=255)
                                                                                                  gaCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTg   >  2:4109241/1‑100 (MQ=255)
                                                                                                   |                                                                                                    
TGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGC  >  NC_000913/4520401‑4520599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: