Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,712,518 (C)6→5 100% intergenic (+360/‑251) nth → / → dtpA DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,712,5130C.97.0% 599.6 / 7.2 135intergenic (+355/‑256)nth/dtpADNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base . (68/63);  total (69/66)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 144 total aligned reads displayed.

TGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCA  >  NC_000913/1712417‑1712608
                                                                                                |                                                                                               
tGACCTACAAAACATTACACTGGCTATTTTTTTTTTTTTTTACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCTATCAccc                                                                                               >  1:1140511/1‑99 (MQ=255)
tGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAcccc                                                                                              >  2:4005857/1‑100 (MQ=255)
  aCCTACAAAACATTACACTGGCTATTTTTCAGAAAATGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACCTATCGCCCTTAACCCATCaccacc                                                                                             >  1:1245601/1‑99 (MQ=255)
   ccTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctc                                                                                           >  2:2293086/1‑98 (MQ=255)
   ccTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctc                                                                                           >  1:2747476/1‑98 (MQ=255)
      aCAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTca                                                                                       >  2:2774596/1‑100 (MQ=255)
       cAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAACCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcag                                                                                      <  1:1383324/100‑1 (MQ=255)
       cAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcag                                                                                      <  2:1485607/100‑1 (MQ=255)
        aaaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcag                                                                                      >  1:4226655/1‑99 (MQ=255)
         aaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtg                                                                                    <  2:4150210/100‑1 (MQ=255)
         aaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtg                                                                                    >  2:3331303/1‑100 (MQ=255)
         aaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtg                                                                                    >  1:290792/1‑100 (MQ=255)
         aaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtg                                                                                    >  2:3268031/1‑100 (MQ=255)
          aaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgc                                                                                   <  2:3436741/100‑1 (MQ=255)
            cATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgcag                                                                                 <  2:1128654/100‑1 (MQ=255)
             aTTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgcagt                                                                                <  2:3074472/100‑1 (MQ=255)
             aTTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgcagt                                                                                >  1:2781552/1‑100 (MQ=255)
              ttACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgcagtg                                                                               <  2:1973281/100‑1 (MQ=255)
              ttACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgcagtg                                                                               <  1:3694255/100‑1 (MQ=255)
                atacTGGCTATTTATCAGAAACTGGACTATCACACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaa                                                                             >  2:2879943/3‑100 (MQ=255)
                 cacTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaa                                                                            <  1:2620179/100‑1 (MQ=255)
                 cacTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaa                                                                            >  1:4261706/1‑100 (MQ=255)
                  acTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaaa                                                                           >  2:2594563/1‑100 (MQ=255)
                    tGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaaaaa                                                                         >  2:1904215/1‑100 (MQ=255)
                     ggCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAAt                                                                        >  1:2746569/1‑100 (MQ=255)
                      gCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATc                                                                       <  1:694008/100‑1 (MQ=255)
                      gCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATc                                                                       >  2:3357910/1‑100 (MQ=255)
                       cTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCt                                                                      <  2:3081704/100‑1 (MQ=255)
                        tATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTg                                                                     <  2:2170363/100‑1 (MQ=255)
                        tATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCAATCACACCCTCTCAGTGCAGTGAAAAAATCTg                                                                     >  2:502885/1‑100 (MQ=255)
                         aTTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGc                                                                    >  1:3937355/1‑100 (MQ=255)
                            tttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGt                                                                 <  1:4118005/100‑1 (MQ=255)
                               cAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTa                                                               >  1:3945833/1‑100 (MQ=255)
                               cAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTAc                                                              >  1:3174512/1‑100 (MQ=255)
                               cAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTAc                                                              >  1:813476/1‑100 (MQ=255)
                                aGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACg                                                             <  2:239213/100‑1 (MQ=255)
                                 gAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGt                                                            <  2:3642658/100‑1 (MQ=255)
                                 gAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGt                                                            >  2:3931982/1‑100 (MQ=255)
                                 gAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGt                                                            >  1:1190310/1‑100 (MQ=255)
                                      tGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGtttttt                                                       <  1:2049376/100‑1 (MQ=255)
                                      tGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGtttttt                                                       <  1:1091603/100‑1 (MQ=255)
                                       ggACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTg                                                      <  1:4196336/100‑1 (MQ=255)
                                       ggACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAAAGAAATAAAAAAATCTGCCGTTACGTTTTTTg                                                      <  1:1904211/100‑1 (MQ=255)
                                       ggACGATCTTACTATTAAAAACGCAAAATAGCAGAACATATCTCCGTTAAGCCATCACCCCCTCTCAGGGCAGTGAAAAAATCTGCCGTTACGtttttt                                                       <  2:936984/99‑1 (MQ=255)
                                        gACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTg                                                      <  1:960344/99‑1 (MQ=255)
                                        gACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGa                                                     <  1:1435584/100‑1 (MQ=255)
                                          cTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGaaa                                                   <  2:1495542/100‑1 (MQ=255)
                                            aTCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGaaaaa                                                 >  1:3898833/1‑100 (MQ=255)
                                              ctcACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCGGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAAtt                                               >  1:1267785/1‑100 (MQ=255)
                                              ctcACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAAtt                                               >  2:902201/1‑100 (MQ=255)
                                               tcACTAGTAAAAACGCTAGATAGCAGAACATATCGACGTTAAGCCATCACCCTCTGTCAGTGCAGTCAAAAAATCTGAGGTTACGTTTTTTAaaaagt                                                >  1:4079253/1‑96 (MQ=255)
                                                cACTAGTAAAAACGTTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                             >  1:2313565/1‑100 (MQ=255)
                                                cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                             <  2:3122385/100‑1 (MQ=255)
                                                cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                             >  2:1998472/1‑100 (MQ=255)
                                                 aCTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTaa                                            >  2:2861521/1‑100 (MQ=255)
                                                    aGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGc                                         >  2:1320062/1‑100 (MQ=255)
                                                    aGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGc                                         <  1:346931/100‑1 (MQ=255)
                                                    aGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGc                                         <  1:2861104/100‑1 (MQ=255)
                                                      tAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTg                                       >  2:2770681/1‑100 (MQ=255)
                                                      tAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTg                                       >  2:2009134/1‑100 (MQ=255)
                                                       aaaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTgg                                      >  1:3477473/1‑100 (MQ=255)
                                                        aaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGa                                     <  2:110673/100‑1 (MQ=255)
                                                        aaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCGCAGTGCAGTGAAAAAATCTGCCGTTACGTGGTTTGAAAAAGTTAACGCTgg                                      >  2:1261351/1‑99 (MQ=255)
                                                           aCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATaa                                  <  1:1009683/100‑1 (MQ=255)
                                                            cGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAAc                                 <  1:2889019/100‑1 (MQ=255)
                                                             gCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACa                                <  2:3898851/100‑1 (MQ=255)
                                                             gCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACa                                <  2:353203/100‑1 (MQ=255)
                                                             gCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACa                                <  2:2695222/100‑1 (MQ=255)
                                                             gCTAAATAGAAGAAAATATCGCCGTTAAGCCATCACCCTATAAAAAAAAAAAAAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACa                                <  1:1005909/100‑1 (MQ=255)
                                                              cTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACAt                               <  2:37941/100‑1 (MQ=255)
                                                                aaaTAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACAttt                             <  1:1524025/100‑1 (MQ=255)
                                                                 aaTAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTTTTTATTTTAACGCTGTATAACAt                               >  1:1695761/1‑97 (MQ=255)
                                                                 aaTAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGAGCAGTGAAAAAATCTGCCGTAAAAAAAAAAAAAAAATTTAACGCTGGATAACATTTc                            <  1:1634800/100‑1 (MQ=255)
                                                                  aTAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTcc                           >  1:2095724/1‑100 (MQ=255)
                                                                    aacAGAACATATCTACGTTAAGCCATTATCCTCTCTCAAAAAAAAAAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTcc                           <  1:1300571/96‑1 (MQ=255)
                                                                    aGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTccc                          <  1:4163798/99‑1 (MQ=255)
                                                                     gCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCgg                        <  2:261035/100‑1 (MQ=255)
                                                                     gCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCgg                        >  1:2464442/1‑100 (MQ=255)
                                                                     gCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCgg                        <  2:2044771/100‑1 (MQ=255)
                                                                     gCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTGGAAAAATTTAACGCTGGATAACATTTCCCgg                        <  1:2002615/100‑1 (MQ=255)
                                                                       aGAACATATCGCCGTTAAGCCATCACCCCCTCACAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGaa                      >  2:4059012/1‑100 (MQ=255)
                                                                        gAACATATCGCGGTTAAGCCATCACCCCCTCTCAATGCAGTGAAAAAATCCGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAAAATTTCCCGGaa                      >  1:3244832/1‑99 (MQ=255)
                                                                            atatCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGt                  <  2:3945851/100‑1 (MQ=255)
                                                                             tatCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAAttcaac                >  2:68505/1‑95 (MQ=255)
                                                                              atCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGa               <  2:3477486/100‑1 (MQ=255)
                                                                                cGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTTAAt             >  2:501740/1‑100 (MQ=255)
                                                                                    gTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAATAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTccc         >  1:59135/1‑100 (MQ=255)
                                                                                       aaGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCTGTTACGTTTTTTGAAAAATCTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGc      <  1:2329443/100‑1 (MQ=255)
                                                                                       aaGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGc      <  2:186832/100‑1 (MQ=255)
                                                                                        aGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGcc     <  1:4239813/100‑1 (MQ=255)
                                                                                        aGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGcc     <  2:577726/100‑1 (MQ=255)
                                                                                         gCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCt    >  1:1590648/1‑100 (MQ=255)
                                                                                         gCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCt    >  2:3256638/1‑100 (MQ=255)
                                                                                         gCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCt    >  1:519094/1‑100 (MQ=255)
                                                                                          ccATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTc   >  2:356560/1‑100 (MQ=255)
                                                                                          ccATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTc   >  2:416230/1‑100 (MQ=255)
                                                                                          ccATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTc   >  1:3078792/1‑100 (MQ=255)
                                                                                          ccATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTACCCGCCTc   >  2:352436/1‑100 (MQ=255)
                                                                                           cATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCa  >  1:1352935/1‑100 (MQ=255)
                                                                                           cATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGATGGATAACATTTCCCGGAATGGTTTAATTCCCCGCCTCa  >  1:3475515/1‑100 (MQ=255)
                                                                                                |                                                                                               
TGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCA  >  NC_000913/1712417‑1712608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: