Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,427,065:1	+A	100%	intergenic (‑32/+11)	alaU ← / ← ileU	tRNA‑Ala/tRNA‑Ile

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,427,065	1	.	A	60.0%	‑6.1 / 2.7	5	intergenic (‑32/+11)	alaU/ileU	tRNA‑Ala/tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base A (0/3);  total (0/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGC‑CGTGCAAATTTGGTAG  >  NC_000913/3426977‑3427081                                                                                          |                 cGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCACG‑GCAAAttt       <  1:3341829/100‑1 (MQ=1) cGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCACG‑GCAAAttt       <  2:1635714/100‑1 (MQ=1) cGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCACG‑GCAAAttt       <  2:4004250/100‑1 (MQ=1)   agcctcAGACCTCTTGCGGGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGACGCATAACATGTAGTTAAAACCTCTTCAAATTTGC‑ACGGCAAATTTg      <  1:220575/94‑1 (MQ=1)      cggaGACGTGCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTAGAGCCCCATAACATGTAGTTAAAACCTCGTCAAATTTGC‑ACGGCAAATTTgaaga  <  1:1837425/97‑5 (MQ=1)                                                                                          |                 CGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGC‑CGTGCAAATTTGGTAG  >  NC_000913/3426977‑3427081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑