Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 497,139:1 (T)5→6 100% intergenic (+145/‑36) htpG → / → adk protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913497,1341.T95.3% 494.6 / 15.2 148intergenic (+140/‑41)htpG/adkprotein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
Reads supporting (aligned to +/- strand):  ref base . (4/3);  new base T (60/80);  total (64/84)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

TCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAG  >  NC_000913/497037‑497228
                                                                                                   |                                                                                                
tcatcaTCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtt                                                                                               >  1:2162734/1‑100 (MQ=255)
tcatcaTCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGCGGTATCGTTTATCGCtt                                                                                               >  1:778372/1‑100 (MQ=255)
 catcatCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttt                                                                                             >  2:4085580/1‑100 (MQ=255)
 catcatCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttt                                                                                             >  2:3704615/1‑100 (MQ=255)
 catcatCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttt                                                                                             >  2:3270843/1‑100 (MQ=255)
   tcatcTTCACTTACAACCAATTATCTCGTCACTAACCTTTTGAGCCCCAGGTGCCTTTCTTGACGCAATCGCCTGTTGGTGGTATCGTTTATCGCtt                                                                                               <  1:1200633/97‑1 (MQ=255)
     atcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTCATCGCttttttc                                                                                         <  2:2808620/100‑3 (MQ=255)
      tcTGCCCTTTCCGCAAATTATCTCCCCATTAACCGTTTCTGCCCCAGATACCTTTCTTGAGGCAATCGCCTGGTGGTGGTATCGTTTATCGCTTCTTTCa                                                                                         >  1:3903787/1‑100 (MQ=255)
       cTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttcaa                                                                                       >  2:1232914/1‑96 (MQ=255)
        tGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttcaaa                                                                                      >  1:3010352/1‑95 (MQ=255)
            cTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAAt                                                                                   <  2:806139/100‑1 (MQ=255)
            cTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAAt                                                                                   >  1:810780/1‑100 (MQ=255)
              ttCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTc                                                                                 <  2:2469532/100‑1 (MQ=255)
              ttCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTc                                                                                 <  2:127905/100‑1 (MQ=255)
              ttCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTc                                                                                 <  1:1661155/100‑1 (MQ=255)
               tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  2:1681564/100‑1 (MQ=255)
               tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  2:2683500/100‑1 (MQ=255)
               tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  2:311014/100‑1 (MQ=255)
                 cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              >  2:1054909/1‑100 (MQ=255)
                 cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              >  2:3087051/1‑100 (MQ=255)
                 cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              >  1:3118582/1‑100 (MQ=255)
                 cGCAAATTATCTCGACATTAACCGGTTCAGCCCCAAGTGACTTTCTTGAGGCAATCGACTGTTGGTTGTATCGTTTATCGCTTTTTTCAAAGAATTCGa                                                                               >  1:1960676/1‑99 (MQ=255)
                   cAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGacac                                                                            >  2:1875725/1‑100 (MQ=255)
                   cAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGacac                                                                            <  2:870285/100‑1 (MQ=255)
                    aaaTTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGAcaca                                                                           >  1:4216112/1‑100 (MQ=255)
                     aaTTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACAt                                                                          <  1:4168113/100‑1 (MQ=255)
                      aTTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACAtt                                                                         <  1:972310/100‑1 (MQ=255)
                        tATCTCGCCATTAACCGTTTCAGCCCCAGGCGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACAtttt                                                                       >  1:3672978/1‑100 (MQ=255)
                            tcGCCATTAACCGTTTCCGTCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGCTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAgg                                                                   <  1:3998609/100‑1 (MQ=255)
                             cGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAgtg                                                                  <  1:1011922/100‑3 (MQ=255)
                             cGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAggg                                                                  <  1:3094025/100‑1 (MQ=255)
                             cGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAggg                                                                  >  2:4177857/1‑100 (MQ=255)
                               ccATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGa                                                                >  2:3931515/1‑100 (MQ=255)
                                   tAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAtttt                                                            >  2:3446050/1‑100 (MQ=255)
                                   tAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAtttt                                                            >  2:3440218/1‑100 (MQ=255)
                                    aaCCGTTTCAGCCCCAGGTGCCTTTCTTTAGGCAATCGCCTGTTTGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTc                                                           <  1:3599686/100‑1 (MQ=255)
                                    aaCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTc                                                           >  2:2082506/1‑100 (MQ=255)
                                    aaCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTc                                                           >  2:3861219/1‑100 (MQ=255)
                                     aCCGTTTCAGCCCCAGGTGCCTTTCTTGCGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAGAATTCGACACATTTTAAGGGGATTTTCg                                                          <  1:3447205/100‑1 (MQ=255)
                                     aCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCg                                                          >  2:2097804/1‑100 (MQ=255)
                                     aCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCg                                                          <  2:2309211/100‑1 (MQ=255)
                                      ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         <  1:2430463/100‑1 (MQ=255)
                                      ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         >  2:223508/1‑100 (MQ=255)
                                          ttCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATg                                                     >  2:690439/1‑100 (MQ=255)
                                           tCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg                                                   <  2:1520283/100‑1 (MQ=255)
                                            cAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg                                                   <  2:3283670/100‑1 (MQ=255)
                                            cAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg                                                   >  2:2362420/1‑100 (MQ=255)
                                              gCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTa                                                 >  2:2533525/1‑100 (MQ=255)
                                               ccccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTAt                                                <  2:475399/100‑1 (MQ=255)
                                                cccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAACATTCGACACATTTTAAGGGGATTTTCGCAATCCGTATc                                               >  1:4234849/1‑100 (MQ=255)
                                                cccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAATTTCGACACATTTTAAGGGGATTTTCGCAATGCGTATc                                               >  2:2967294/1‑100 (MQ=255)
                                                cccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTAt                                                >  1:3660251/1‑99 (MQ=255)
                                                cccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATc                                               <  1:2114146/100‑1 (MQ=255)
                                                cccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATc                                               <  2:2475607/100‑1 (MQ=255)
                                                    ggTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTc                                           >  1:3250676/1‑100 (MQ=255)
                                                     gTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATc                                               <  1:4274854/95‑1 (MQ=255)
                                                       gCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGATTTTTTCAAAACATTCGACACATTTTAAGGGGATTTTCGCAATACGTATCGTTCTGc                                        >  1:3731248/1‑100 (MQ=255)
                                                        ccTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCt                                       >  2:2328586/1‑100 (MQ=255)
                                                        ccTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCt                                       >  1:333263/1‑100 (MQ=255)
                                                          tttCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTTTTTTTTTTTTACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCtt                                      >  1:834714/1‑99 (MQ=255)
                                                            tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTTAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  2:705495/100‑1 (MQ=255)
                                                            tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  2:1842167/100‑1 (MQ=255)
                                                            tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  2:3424212/100‑1 (MQ=255)
                                                            tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  1:675468/100‑1 (MQ=255)
                                                               tGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCt                                >  2:3600209/1‑100 (MQ=255)
                                                               tGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCt                                >  1:3432037/1‑100 (MQ=255)
                                                                gAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTTTTTTTTTTTTACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgcg                                  >  1:1389460/1‑97 (MQ=255)
                                                                  ggCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTTTTTTTTTTTGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCt                                >  1:2518886/1‑97 (MQ=255)
                                                                  ggCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCg                             >  1:1607822/1‑100 (MQ=255)
                                                                    cAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCggg                           >  2:112552/1‑100 (MQ=255)
                                                                      aTCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgcg                         >  2:2488286/1‑100 (MQ=255)
                                                                        cGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGcgcg                       >  1:1030128/1‑100 (MQ=255)
                                                                         gcattgtggtgaTATTGTTAATCGATATTTTCAGAAAATTCGATGCATCTTAAGGGCGTTATCGCAATGCCTATCATTCTGCTTGGCGCTCCggg                           <  1:2646890/89‑1 (MQ=255)
                                                                         gCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGACTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgg                      <  1:2336295/100‑1 (MQ=255)
                                                                           cTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgggg                    <  2:2541536/100‑1 (MQ=255)
                                                                             gTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGaa                  >  1:1173863/1‑100 (MQ=255)
                                                                               tggtggTATCTTTTATGGCTATATGAAAAAAATTCGACACATTGTAAGGGGAGTTTCGCAAGGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAg                <  1:2623339/100‑1 (MQ=255)
                                                                               tggtggTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAg                >  1:3912605/1‑100 (MQ=255)
                                                                               tggtggTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAg                <  2:3797838/100‑1 (MQ=255)
                                                                               ccgatctATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAg                <  2:2419549/94‑1 (MQ=255)
                                                                                ggtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAgg               <  1:1395703/100‑1 (MQ=255)
                                                                                ggtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAgg               <  1:608038/100‑1 (MQ=255)
                                                                                 gtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg              >  2:1638331/1‑100 (MQ=255)
                                                                                 gtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg              <  1:1675334/100‑1 (MQ=255)
                                                                                 gtggtATCGTTTATCGCGTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg              <  2:55888/100‑1 (MQ=255)
                                                                                   ggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGAc            <  1:107145/100‑1 (MQ=255)
                                                                                   ggtATCGTTTATCGCTTTTTTCAAAAAATTCGAAACATTTTAAGGGGATTTTCGCAATGCGTAGCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGAc            >  1:567891/1‑100 (MQ=255)
                                                                                   ggtATCGTTTAGCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGAc            <  1:3983690/100‑1 (MQ=255)
                                                                                    gtATCGTTTATCGCTTTTTTCAAAGAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACt           <  2:2010899/100‑1 (MQ=255)
                                                                                      aTCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAgagatcgg         >  1:2419543/1‑93 (MQ=255)
                                                                                      aTCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGAGGAAAGGGACtga         <  2:2874505/100‑3 (MQ=255)
                                                                                        cGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAgg       <  2:3183871/100‑1 (MQ=255)
                                                                                         gTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGc      <  2:1362640/100‑1 (MQ=255)
                                                                                         gTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGc      >  1:1211115/1‑100 (MQ=255)
                                                                                         gTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTACCATTCTGCTTCGCGCTCCGGGCGCGGGGTAAGGGACTCAgcc      <  2:2887104/100‑3 (MQ=255)
                                                                                           ttATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTc    <  2:1224850/100‑1 (MQ=255)
                                                                                           ttATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTc    >  2:2447866/1‑100 (MQ=255)
                                                                                            tATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTc    <  1:4085558/99‑1 (MQ=255)
                                                                                            tATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCa   <  2:3672994/100‑1 (MQ=255)
                                                                                             aTCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAg  >  2:2294183/1‑100 (MQ=255)
                                                                                                   |                                                                                                
TCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAG  >  NC_000913/497037‑497228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: