Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,520,504:1 +T 15.1% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5041.T15.1% 705.1 / 134.4 212intergenic (‑180/+167)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base . (89/91);  new base T (18/14);  total (107/105)
Fisher's exact test for biased strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

Only 100 of 214 total aligned reads displayed.

ATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAC  >  NC_000913/4520405‑4520603
                                                                                                    |                                                                                                   
   aTTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTc                                                                                                  >  2:3402797/1‑100 (MQ=255)
       tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGc                                                                                              <  2:3907422/100‑1 (MQ=255)
       tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGc                                                                                              >  1:1365322/1‑100 (MQ=255)
        ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCt                                                                                             >  2:1484210/1‑100 (MQ=255)
        ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCt                                                                                             <  2:1789452/100‑1 (MQ=38)
         taCTGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCtt                                                                                            <  1:3808739/100‑1 (MQ=255)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCtt                                                                                            <  2:1910096/100‑1 (MQ=255)
          aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCttt                                                                                           <  1:3151358/100‑1 (MQ=255)
          aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCttt                                                                                           <  2:2161310/100‑1 (MQ=255)
          aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCttt                                                                                           >  2:3984931/1‑100 (MQ=255)
          aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCttt                                                                                           <  1:4254880/100‑1 (MQ=255)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGGGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                          <  1:683985/100‑1 (MQ=255)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                          >  1:737139/1‑100 (MQ=255)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                          >  1:2078443/1‑100 (MQ=255)
            aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTAc                                                                                         <  2:835562/100‑1 (MQ=255)
            aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCTGGCTTTAc                                                                                         <  2:3358629/100‑1 (MQ=38)
            aGATATTTTATTCTATTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTAc                                                                                         <  1:2908898/100‑1 (MQ=255)
             gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCTATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATACTCCGGCTTTACa                                                                                        >  1:1000322/1‑100 (MQ=255)
             gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACa                                                                                        >  2:1199215/1‑100 (MQ=255)
                 ttttATTCTTTTACATGAAATAAACCAGCAAACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCTGGCTTTACAGTAt                                                                                    >  1:2809342/1‑100 (MQ=255)
                    tATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTAc                                                                                 <  2:3344112/100‑1 (MQ=255)
                      ttCTTTTACATGAAATAAACCAGCATACTATTTGAGCGATCGCCAGATGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTAACAGTATTACTg                                                                               >  2:1358352/1‑100 (MQ=255)
                        cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgag                                                                             <  1:3242344/100‑1 (MQ=255)
                              naTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                       >  1:2089937/2‑100 (MQ=255)
                              cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                       >  1:2142451/1‑100 (MQ=255)
                               aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATacac                                                                      >  2:563502/1‑100 (MQ=255)
                                 gAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAccc                                                                    >  2:2335202/1‑100 (MQ=255)
                                   aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCAt                                                                  >  2:1618851/1‑100 (MQ=255)
                                    aTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATc                                                                 >  2:568617/1‑100 (MQ=255)
                                     tAAACCAGCATACCATTTGAGCGATCGCCCGCTTTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATc                                                                 <  1:1628520/99‑1 (MQ=255)
                                     tAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCa                                                                <  1:3866597/100‑1 (MQ=255)
                                      aaaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                               <  1:3498318/100‑1 (MQ=255)
                                       aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                              <  1:3426279/100‑1 (MQ=255)
                                       aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                              <  1:1274455/100‑1 (MQ=255)
                                       aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                              >  2:3283601/1‑100 (MQ=255)
                                        aCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGc                                                             >  1:3325067/1‑100 (MQ=255)
                                        aCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGc                                                             <  2:3544980/100‑1 (MQ=255)
                                         ccAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                            <  2:1634311/100‑1 (MQ=255)
                                            gCATACCATTTGAGCGATCGCTCGCTGTTTCCGCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCAt                                                           >  1:1731611/1‑98 (MQ=255)
                                              aTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                       <  1:1760567/100‑1 (MQ=255)
                                               tACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                      >  2:3846010/1‑100 (MQ=255)
                                                aCCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                       <  1:715520/98‑1 (MQ=255)
                                                 ccATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                    <  2:1106740/100‑1 (MQ=255)
                                                  cATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg                                                   <  2:1716401/100‑1 (MQ=255)
                                                   aTTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAgg                                                  >  2:729344/1‑100 (MQ=255)
                                                   aTTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAgg                                                  >  2:639536/1‑100 (MQ=255)
                                                      tctgCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTc                                               <  1:2490048/97‑1 (MQ=255)
                                                      tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTc                                               <  2:2680456/100‑1 (MQ=255)
                                                      tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTc                                               <  2:284775/100‑1 (MQ=255)
                                                       gAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCa                                              >  2:4152630/1‑100 (MQ=255)
                                                         gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCaga                                            >  2:2490054/1‑98 (MQ=255)
                                                         gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                            >  1:748173/1‑100 (MQ=255)
                                                           ggtCGCCCGCTGTGCACACATACGGTAAAGCCAGAGACATGCGACGGCTTTACAGACTTACTCAGAATAGACCCATCCAGCATAATCGAAGGTCCAat                                            >  1:2174326/3‑98 (MQ=255)
                                                           gagcGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCa                                              <  1:1240412/93‑1 (MQ=255)
                                                            aTCGCCCGCTGTTCCCCCATACTGTAGAGCCGGAGACATGCTCCGGCTTTACAGTGTTACTGGGAATAAACCCATCAAGGCTAATCGAAGGTTCaag                                            >  1:1904823/1‑96 (MQ=255)
                                                            aTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATc                                         >  1:599889/1‑100 (MQ=255)
                                                              cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                       <  2:2238678/100‑1 (MQ=255)
                                                              cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                       >  1:3255163/1‑100 (MQ=255)
                                                               gaccGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATTATCGAAGGTTCAATATCGcc                                      <  1:2598498/98‑1 (MQ=255)
                                                                cccGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGccc                                     >  2:2177845/1‑100 (MQ=255)
                                                                     tGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                                <  2:236813/100‑1 (MQ=255)
                                                                      gTTCCCCCATACTGTAAGGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAAGACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                               >  1:194257/1‑100 (MQ=255)
                                                                      gTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                               <  2:4176108/100‑1 (MQ=255)
                                                                      gTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCAttttt                               >  2:331936/1‑100 (MQ=255)
                                                                       ttCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTg                              <  1:3726704/100‑1 (MQ=255)
                                                                       ttCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTg                              <  2:324919/100‑1 (MQ=255)
                                                                        tCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                             >  2:3185901/1‑100 (MQ=255)
                                                                         cccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  1:2724997/1‑100 (MQ=255)
                                                                         cccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  2:2580095/1‑100 (MQ=255)
                                                                          ccccATCCTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                           <  1:2689880/100‑1 (MQ=255)
                                                                          ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                           >  1:1787042/1‑100 (MQ=255)
                                                                                 cTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                    >  2:1878457/1‑100 (MQ=255)
                                                                                 cTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                    >  1:3446333/1‑100 (MQ=255)
                                                                                 cTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                    <  1:4152608/100‑1 (MQ=255)
                                                                                 cTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                    >  2:2531741/1‑100 (MQ=255)
                                                                                 cTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                     >  1:846546/1‑99 (MQ=38)
                                                                                  tGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATAGCCCCTTTTTGATCACCAGCGa                   >  1:779037/1‑100 (MQ=255)
                                                                                  tGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAAAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATAGCCAGCGa                   >  1:2744510/1‑100 (MQ=37)
                                                                                   gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGTTCCAATATCGCCCCTTTTTGCTCGCCAGCGAt                  >  2:3454424/1‑100 (MQ=255)
                                                                                   gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCCATATCGCCCCTTTTTGATCCCCCGc                     >  1:2403821/1‑97 (MQ=255)
                                                                                   gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                  >  1:906494/1‑100 (MQ=255)
                                                                                   gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATAGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                  >  2:326881/1‑100 (MQ=255)
                                                                                   gTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                  >  1:1207936/1‑100 (MQ=38)
                                                                                    tAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                  >  1:2946064/1‑99 (MQ=38)
                                                                                     aaaGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAAACGAAGGTTCAATATCGACCCTTTTTGATCGCCAGCGACTc                >  2:1627107/1‑100 (MQ=37)
                                                                                      aaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               >  2:1560435/1‑100 (MQ=255)
                                                                                      aaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               <  2:1048786/100‑1 (MQ=255)
                                                                                      aaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               <  1:4030224/100‑1 (MQ=255)
                                                                                      aaGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGCATACACCCATCAAGCATAATCGAAGGATCCATATCGCCCCTTTTTGATAGCCAGCGa                   >  1:554078/1‑96 (MQ=25)
                                                                                       aGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGt              <  2:2078447/100‑1 (MQ=255)
                                                                                       aGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGt              >  1:1227775/1‑100 (MQ=38)
                                                                                        gCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTc             <  1:1084353/100‑1 (MQ=255)
                                                                                           ggAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCAcc          >  2:3063836/1‑100 (MQ=255)
                                                                                            gagaCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCg         <  1:486399/100‑1 (MQ=255)
                                                                                                cATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGc      >  2:1179276/1‑99 (MQ=255)
                                                                                                cATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa     <  2:3588248/100‑1 (MQ=255)
                                                                                                cATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCAAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa     >  1:1499080/1‑100 (MQ=255)
                                                                                                  tGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGa   >  1:265305/1‑100 (MQ=255)
                                                                                                  tGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGa   >  2:3771131/1‑100 (MQ=255)
                                                                                                   gCTGCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAc  <  1:1838610/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
ATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAC  >  NC_000913/4520405‑4520603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: