New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4625174 =213 (1.390)8 (0.060) 4/166 NT 4.0% coding (280/969 nt) serB 3‑phosphoserine phosphatase
?NC_000913 4625219 = 181 (1.260)coding (325/969 nt) serB 3‑phosphoserine phosphatase
Rejected: Frequency below cutoff threshold.

ATCAATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4625278‑4625174
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgcgcACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCG  >  NC_000913/4625219‑4625317
                                                                                                                                                                                                            
ATCAATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGC                                                                                                          <  2:1503992/100‑1
ATCAATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGC                                                                                                          >  2:2863151/1‑100
ATCAATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGC                                                                                                          >  2:3704148/1‑100
 TCAATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCT                                                                                                         >  2:1712803/1‑100
 TCAATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCT                                                                                                         >  1:1640331/1‑100
   AATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGC                                                                                                       <  1:1823992/100‑1
   AATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGC                                                                                                       <  1:274863/100‑1
   AATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGC                                                                                                       >  2:4186689/1‑100
   AATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGC                                                                                                       <  2:3012793/100‑1
   AATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGC                                                                                                       <  1:1381315/100‑1
   AATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGC                                                                                                       >  2:1305544/1‑100
   AATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGC                                                                                                       >  2:608982/1‑100
   AATACACTCAATCTGGATGGCGGTGGAGTCCAAATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTG                                                                                                        <  1:3838594/99‑1
    ATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCG                                                                                                      <  2:3905599/100‑1
    ATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCG                                                                                                      <  2:3401855/100‑1
    ATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCG                                                                                                      <  2:2307552/100‑1
    ATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCG                                                                                                      <  1:1256511/100‑1
    ATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCG                                                                                                      >  1:2979992/1‑100
                                                     CCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATT                                                     >  2:2760221/1‑100
                                                       GGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGA                                                   >  1:121320/1‑100
                                                         CGTGCGCAGGTGCGGGATTTTCGCCAGCGGGGCGACATCCAGCTGCGCACGCCGGGATTGCTGGTGATGGATATGGGCGCCACCGCCATCCAGACTGCAT                                                 >  1:925110/1‑100
                                                         CGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCGCACGCCGGGTTTGCTGGTGATGGCTATGGACTCCACCGCCATCCAGATTGAAT                                                 >  1:2038354/1‑100
                                                          GTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGGCATCCAGCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATG                                                >  1:2820554/1‑100
                                                          GTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATG                                                >  2:1681124/1‑100
                                                          GTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATG                                                >  1:1533723/1‑100
                                                          GTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCGCACGCCGGGTTTGCTGGGGATGGATATGGACTCCACCGCCATCCAGATTGAATG                                                >  2:1907852/1‑100
                                                                                                    TGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGTCAAACTGGCCGGAACGGGCGAGATGGT      >  2:147824/1‑100
                                                                                                     GCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTG     >  1:4199737/1‑100
                                                                                                       GCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGC   <  2:3284071/100‑1
                                                                                                       GCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGC   <  2:842891/100‑1
                                                                                                        CACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCG  <  2:2514655/100‑1
                                                                                                                                                                                                            
ATCAATACATTCAATCTGGATGGCGGTGGAGTCCATATCCATCACCAGCAAACCCGGCGTGCGCAGGTGCGGGATTTTCCCCAGCGGGGCGACATCCAGCTGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4625278‑4625174
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgcgcACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCG  >  NC_000913/4625219‑4625317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.