Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,809,610 A→G 42.3% E77E (GAA→GAG yniC → 2‑deoxyglucose‑6‑P phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,809,6100AG42.3% 49.4 / 175.0 142E77E (GAA→GAGyniC2‑deoxyglucose‑6‑P phosphatase
Reads supporting (aligned to +/- strand):  ref base A (50/32);  new base G (31/29);  total (81/61)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.90e-01

Only 100 of 142 total aligned reads displayed.

AGCTGCCGGACACCTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTAT  >  NC_000913/1809513‑1809707
                                                                                                 |                                                                                                 
aGCTGCCGGACACCTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCg                                                                                                 <  2:3027023/100‑1 (MQ=255)
aGCTGCCGGACACCTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCg                                                                                                 <  1:3311541/100‑1 (MQ=255)
  cTGCCGGACACCTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACggg                                                                                               >  2:87194/1‑100 (MQ=255)
    gCCGGACACCTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGtt                                                                                             >  2:370745/1‑100 (MQ=255)
      cGGACACCTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTAt                                                                                           >  2:1798742/1‑100 (MQ=255)
      cGGACACCGTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTa                                                                                            <  1:412156/99‑1 (MQ=255)
       ggACACCTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTAtt                                                                                          >  1:4176864/1‑100 (MQ=255)
          cacCTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGcc                                                                                       >  2:812998/1‑100 (MQ=255)
            ccTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCg                                                                                     >  2:508940/1‑100 (MQ=255)
            ccTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCg                                                                                     >  2:1335759/1‑100 (MQ=255)
            ccTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCg                                                                                     >  2:893703/1‑100 (MQ=255)
            ccTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCg                                                                                     >  2:1517006/1‑100 (MQ=255)
              ttAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTg                                                                                   <  2:3454865/100‑1 (MQ=255)
              ttAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTg                                                                                   <  1:1986209/100‑1 (MQ=255)
               tAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGc                                                                                  <  2:1245630/100‑1 (MQ=255)
                 ggTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCa                                                                                >  2:3630344/1‑100 (MQ=255)
                 ggTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTACCCGTGcc                                                                                 >  2:1719213/1‑99 (MQ=255)
                  ntttACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTgtc                                                                                 >  1:2057685/2‑96 (MQ=255)
                  gTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCCGGAAGTAGTAGAACGGGTTATTGCCCGTGCCa                                                                                >  1:2207107/1‑99 (MQ=255)
                  gTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGACGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCAt                                                                               <  1:2627181/100‑1 (MQ=255)
                       cGCATCGATATGGTGGTCGCTCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCAttt                                                                             >  1:1868727/1‑97 (MQ=255)
                       cGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCAc                                                                          <  1:452000/100‑1 (MQ=255)
                       cGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCAc                                                                          >  1:3338093/1‑100 (MQ=255)
                       cGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCAc                                                                          <  1:1615354/100‑1 (MQ=255)
                        gCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACt                                                                         <  1:1983651/100‑1 (MQ=255)
                        gCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACt                                                                         >  2:2504455/1‑100 (MQ=255)
                         cATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTg                                                                        >  2:2364931/1‑100 (MQ=255)
                         cATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTg                                                                        >  1:2951685/1‑100 (MQ=255)
                         cATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTg                                                                        >  1:3149538/1‑100 (MQ=255)
                           tCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGt                                                                      >  2:1049884/1‑100 (MQ=255)
                            cGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGtt                                                                     <  1:779018/100‑1 (MQ=255)
                             gATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTg                                                                    >  2:1763843/1‑100 (MQ=255)
                             gATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTg                                                                    >  1:3847701/1‑100 (MQ=255)
                              atatGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGa                                                                   >  2:2824988/1‑100 (MQ=255)
                                atGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAag                                                                 >  1:592092/1‑100 (MQ=255)
                                 tggtggTCGATGTTTGGTTCGCCAAGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGAAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAag                                                                 >  1:2708061/1‑99 (MQ=255)
                                 tggtggTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAaga                                                                <  1:2819006/100‑1 (MQ=255)
                                 tggtggTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTACAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAaga                                                                <  1:2280592/100‑1 (MQ=255)
                                 nggtggtCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAaga                                                                >  2:1752856/2‑100 (MQ=255)
                                  ggtggtCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAagag                                                               <  2:450818/100‑1 (MQ=255)
                                  ggtggtCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAagag                                                               >  1:56977/1‑100 (MQ=255)
                                  ggtggtCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAagag                                                               >  1:2873086/1‑100 (MQ=255)
                                  ggtggtCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAagag                                                               >  1:373989/1‑100 (MQ=255)
                                   gtggtCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAgaga                                                              <  2:318492/100‑1 (MQ=255)
                                   gtggtCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAgaga                                                              >  2:1284929/1‑100 (MQ=255)
                                     ggtCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGac                                                             >  1:1499126/1‑99 (MQ=255)
                                     gggcGATCTTGGGTACGCCCGGCGCCTGTCGAGTGGGCCAAGCCGTCAGGGAGTGGGAGAACGGGTTATTGCCCGGGCCATTGCACGGGTTGAAGAGaca                                                            <  1:2696008/97‑1 (MQ=255)
                                      gtCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGacac                                                           >  2:431835/1‑100 (MQ=255)
                                       tCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGACCGTGCCATTTCACTGGTTGAAGAGACACg                                                          >  2:1031235/1‑100 (MQ=255)
                                           tCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCa                                                      <  2:707632/100‑1 (MQ=255)
                                            cTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCat                                                     <  2:2229/100‑1 (MQ=255)
                                               tGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCattat                                                  <  2:2736418/100‑1 (MQ=255)
                                               tGGTACGCCCGGCAACCATGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCattan                                                  <  1:1341882/100‑2 (MQ=255)
                                                ggTACGCCCGGCAACCGTGGAATGGGCCACGCCGTCAGGAAGTAGTAGCACGGGTTATTGCCCGTGCCATTTCACTGGTTGAACAGACACGTCCctt                                                    >  1:3705506/1‑97 (MQ=255)
                                                ggTACGCCCGGCAACCGTGGAAGGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCattatt                                                 <  2:1616388/100‑1 (MQ=255)
                                                 tctcGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCAttatta                                                <  2:2589998/97‑1 (MQ=255)
                                                 gTACGCCCGCGAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGGGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCAttatta                                                <  1:1796781/100‑1 (MQ=255)
                                                  tACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTAc                                               <  1:1915390/100‑1 (MQ=255)
                                                    cGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCAttattaaga                                             >  1:2589990/1‑97 (MQ=255)
                                                     gCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTGTTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAg                                            >  1:1108603/1‑100 (MQ=255)
                                                      cccGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAgg                                           >  2:2728703/1‑100 (MQ=255)
                                                      cccGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAgg                                           >  1:3056675/1‑100 (MQ=255)
                                                      cccGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAgg                                           <  2:2317812/100‑1 (MQ=255)
                                                      cccGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAgg                                           <  2:215046/100‑1 (MQ=255)
                                                      cccGGAAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAgg                                           <  1:1105548/100‑1 (MQ=255)
                                                       ccGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGc                                          >  1:3175474/1‑100 (MQ=255)
                                                            agccGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTgcg                                     <  1:3340412/98‑1 (MQ=255)
                                                             aCCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTgcgc                                    >  1:1248815/1‑100 (MQ=255)
                                                               cGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGa                                  >  1:3413511/1‑100 (MQ=255)
                                                               cGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGa                                  >  2:3835824/1‑100 (MQ=255)
                                                                        ggCCAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGc                         >  2:4090549/1‑100 (MQ=255)
                                                                        ggCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGc                         >  2:4105410/1‑100 (MQ=255)
                                                                        ggCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCAGTATTACCAGGCGTGCGCGAAGCCGTTg                          >  2:2987792/1‑99 (MQ=255)
                                                                          ccAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGt                       <  2:3843562/100‑1 (MQ=255)
                                                                          ccAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGt                       <  2:373991/100‑1 (MQ=255)
                                                                           cAAGCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGtt                      <  2:2915013/100‑1 (MQ=255)
                                                                            aaGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTa                     <  2:2577554/100‑1 (MQ=255)
                                                                             aGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTAt                    >  1:2103189/1‑100 (MQ=255)
                                                                              gCCGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATg                   >  2:1698565/1‑100 (MQ=255)
                                                                              gCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATg                   <  1:256819/100‑1 (MQ=255)
                                                                              gCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATg                   >  1:1743237/1‑100 (MQ=255)
                                                                                cGTCAGGAAGTAGTAGAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCa                 >  1:647901/1‑100 (MQ=255)
                                                                                 gTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCaa                <  1:879932/100‑1 (MQ=255)
                                                                                 gTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCaa                >  2:1462625/1‑100 (MQ=255)
                                                                                   cAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAg              >  2:3022847/1‑100 (MQ=255)
                                                                                     ggAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGaa            >  1:1588014/1‑100 (MQ=255)
                                                                                     ggAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGaa            >  2:3915838/1‑100 (MQ=255)
                                                                                     ggAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGaa            >  2:159303/1‑100 (MQ=255)
                                                                                      gAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAAc           <  1:3409263/100‑1 (MQ=255)
                                                                                       aaGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACa          >  1:3524107/1‑100 (MQ=255)
                                                                                          tagtagAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAgg       <  2:2614791/100‑1 (MQ=255)
                                                                                           agtagAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAgg       <  1:1325890/99‑1 (MQ=255)
                                                                                           agtagAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGt      <  2:1574327/100‑1 (MQ=255)
                                                                                           agtagAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGt      >  1:1877266/1‑100 (MQ=255)
                                                                                           agtagAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGt      >  2:1226567/1‑100 (MQ=255)
                                                                                            gtagAGCGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGtt     >  2:3329803/1‑100 (MQ=255)
                                                                                            gtagAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGtt     >  2:3894828/1‑100 (MQ=255)
                                                                                             tagAGCGGGTTATTGCCGGCGCCCTTGCTCTGGTTGAAGAGACACGCCCATTACTACCAGGAGTGCGCGCAGCGGTTGCGGTATGCAAAGGACAAGGttc    >  1:3062670/1‑99 (MQ=255)
                                                                                               gAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTAt  >  2:1251615/1‑100 (MQ=255)
                                                                                               gAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAACCCGTTGCGTTATGCAAAGAACAAGGTTTAt  >  2:2117674/1‑100 (MQ=255)
                                                                                                 |                                                                                                 
AGCTGCCGGACACCTTAGGTTTACGCATCGATATGGTGGTCGATCTTTGGTACGCCCGGCAACCGTGGAATGGGCCAAGCCGTCAGGAAGTAGTAGAACGGGTTATTGCCCGTGCCATTTCACTGGTTGAAGAGACACGTCCATTATTACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTAT  >  NC_000913/1809513‑1809707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: