Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,827,031 T→A 100% D410V (GAT→GTT)  astB ← succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,827,0310TA98.6% 466.3 / ‑3.1 141D410V (GAT→GTT) astBsuccinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (75/63);  total (75/66)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 141 total aligned reads displayed.

CTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCC  >  NC_000913/1826934‑1827127
                                                                                                 |                                                                                                
ctcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCGCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACg                                                                                                >  2:3005890/1‑100 (MQ=255)
ctcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACg                                                                                                >  2:3462110/1‑100 (MQ=255)
  cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgc                                                                                              <  2:3487451/100‑1 (MQ=255)
  cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgc                                                                                              <  2:1137621/100‑1 (MQ=255)
  cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgc                                                                                              <  2:2056255/100‑1 (MQ=255)
    cgcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcag                                                                                            >  2:1879317/1‑100 (MQ=255)
    cgcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcag                                                                                            <  2:386101/100‑1 (MQ=255)
     gcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGTCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagc                                                                                           <  2:1074045/100‑1 (MQ=255)
     gcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGgcagc                                                                                           <  1:2075826/100‑1 (MQ=255)
      cTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagca                                                                                          >  1:2824435/1‑100 (MQ=255)
       tGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGcagcag                                                                                         <  2:131296/100‑1 (MQ=255)
       tGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGcagcag                                                                                         <  1:3752380/100‑1 (MQ=255)
        ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGcagcag                                                                                         >  2:2103173/1‑99 (MQ=255)
        ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGt                                                                                        >  1:3717254/1‑100 (MQ=255)
        ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGt                                                                                        >  2:3897491/1‑100 (MQ=255)
            cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAgg                                                                                    <  1:1204031/100‑1 (MQ=255)
            cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAgg                                                                                    <  1:632909/100‑1 (MQ=255)
             ggATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGc                                                                                   >  2:3682772/1‑100 (MQ=255)
              gATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCCGCAGCAAGGCg                                                                                  >  1:423693/1‑100 (MQ=255)
              gATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCg                                                                                  >  2:2044908/1‑100 (MQ=255)
                  aaccgaaccgAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcg                                                                              >  1:2663782/1‑100 (MQ=255)
                  aaccgaaccgAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcg                                                                              >  2:3495827/1‑100 (MQ=255)
                  aaccgaaccgAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcg                                                                              >  2:4225861/1‑100 (MQ=255)
                  aaccgaaccgAGATTCAGTAATTGGCTCAATACATCCAGTACTTCCCGCTCGTCGCGCAGCAATTGAGGGTGGGGCAGAACGGCAGCAGTAAGGCGATcg                                                                              >  1:1052310/1‑100 (MQ=255)
                   accgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcgc                                                                             >  1:1123270/1‑100 (MQ=255)
                    ncgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcgcg                                                                            >  2:3328742/2‑100 (MQ=255)
                     cgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCgg                                                                           <  2:1855016/100‑1 (MQ=255)
                     cgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCgg                                                                           <  2:2291069/100‑1 (MQ=255)
                     cgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCgg                                                                           <  1:1408000/100‑1 (MQ=255)
                      gaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgt                                                                          <  2:2406906/100‑1 (MQ=255)
                      gaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgt                                                                          <  1:1763185/100‑1 (MQ=255)
                         ccgaGATTCAGTAATTGGCTCAATGCATCCATTGCTTCCCGCCCTTCGCGCAGCAATTGCGGCCCGGCCAGAACGGCTGCAGTAAGGCGATcgc                                                                             >  1:2518907/1‑94 (MQ=255)
                         ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagt                                                                       >  2:1674364/1‑100 (MQ=255)
                         ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagt                                                                       >  2:4109435/1‑100 (MQ=255)
                         ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGCTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtag                                                                        >  1:604177/1‑99 (MQ=255)
                         ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTACGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagt                                                                       >  1:366634/1‑100 (MQ=255)
                         ccgaGATTCAGTAATTAGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGAACGCGgtagt                                                                       >  2:166884/1‑100 (MQ=255)
                           gagaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTaa                                                                     >  1:3072676/1‑100 (MQ=255)
                            agaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAAc                                                                    <  2:3180/100‑1 (MQ=255)
                             gaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACg                                                                   <  2:1128577/100‑1 (MQ=255)
                              aTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGa                                                                  >  2:3913783/1‑100 (MQ=255)
                                tCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATc                                                                <  1:198550/100‑1 (MQ=255)
                                 cAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATcc                                                               >  1:851094/1‑100 (MQ=255)
                                 cAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATcc                                                               >  2:529578/1‑100 (MQ=255)
                                  aGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCa                                                              <  1:3066768/100‑1 (MQ=255)
                                   gTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAc                                                             >  2:3001200/1‑100 (MQ=255)
                                     aaTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCATCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAccc                                                           <  1:2079930/100‑1 (MQ=255)
                                     aaTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAccc                                                           <  2:1758048/100‑1 (MQ=255)
                                         ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAg                                                         >  1:3226225/1‑98 (MQ=255)
                                         ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTc                                                       >  1:2326602/1‑100 (MQ=255)
                                         ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTc                                                       >  2:1007904/1‑100 (MQ=255)
                                          gCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCa                                                      >  2:1104433/1‑100 (MQ=255)
                                             cAATACAGCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTg                                                   <  1:3986557/100‑1 (MQ=255)
                                               aTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAg                                                 <  1:2612582/100‑1 (MQ=255)
                                                  cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcgc                                              >  2:3090468/1‑100 (MQ=255)
                                                  cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcgc                                              >  2:203345/1‑100 (MQ=255)
                                                  cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcgc                                              >  1:2245197/1‑100 (MQ=255)
                                                   aTCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCa                                             >  2:2950975/1‑100 (MQ=255)
                                                    tCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAt                                            <  2:966385/100‑1 (MQ=255)
                                                     ccAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAtt                                           <  2:2560105/100‑1 (MQ=255)
                                                      cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                          <  1:2500634/100‑1 (MQ=255)
                                                      cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                          >  2:3411992/1‑100 (MQ=255)
                                                      cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                          >  2:3392960/1‑100 (MQ=255)
                                                       aGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTaa                                         >  1:3574916/1‑100 (MQ=255)
                                                          gCTTCCCGCCCTTCGCGCAGCCATTGCGGGTCGGCCAGAACGGCAGAAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGTGCGCATTAAAc                                       <  1:787002/99‑1 (MQ=255)
                                                          gCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACa                                      <  1:4078427/100‑1 (MQ=255)
                                                          gCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACa                                      >  2:32377/1‑100 (MQ=255)
                                                          gCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACa                                      >  2:2125680/1‑100 (MQ=255)
                                                          gCTTCCCGCCCGTCGCGCAGCAAGTGCGGTTCGGCCAGACCGGCAGCATTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAAc                                       <  2:3874067/99‑1 (MQ=255)
                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCGAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCACATTAAACAGCg                                   <  1:3001188/100‑1 (MQ=255)
                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCATAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCATATTTAACAGCg                                   <  1:2242267/100‑1 (MQ=255)
                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                   >  1:1013300/1‑100 (MQ=255)
                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                   <  2:2663792/100‑1 (MQ=255)
                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                   <  1:347973/100‑1 (MQ=255)
                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                   >  1:186050/1‑100 (MQ=255)
                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                   >  2:1650659/1‑100 (MQ=255)
                                                              cccGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGt                                  <  2:3066825/100‑1 (MQ=255)
                                                               ccGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGt                                  <  1:272161/99‑1 (MQ=255)
                                                               ccGCCCTTCGCGCAGCAATTGCGGGCCGGCCAGAACGGCAGCAGTAAGGCGATCGCTGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGc                                    <  1:1052677/97‑1 (MQ=255)
                                                                   ccTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGt                             >  1:3339180/1‑100 (MQ=255)
                                                                    cTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGt                             <  2:1035689/99‑1 (MQ=255)
                                                                       cgcgCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcat                         >  2:2921715/1‑100 (MQ=255)
                                                                       cgcgCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcat                         <  2:3130794/100‑1 (MQ=255)
                                                                       cgcgCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtc                           >  1:3322827/1‑98 (MQ=255)
                                                                        gcgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatc                        <  2:3072688/100‑1 (MQ=255)
                                                                         cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca                       >  2:901296/1‑100 (MQ=255)
                                                                              caATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAcc                  >  2:1763876/1‑100 (MQ=255)
                                                                               aaTTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccg                 <  2:3981096/100‑1 (MQ=255)
                                                                                aTTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgc                >  1:3221436/1‑100 (MQ=255)
                                                                                aTTGCGGGTCGGCCAGAACGGCACCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgc                <  1:16158/100‑1 (MQ=255)
                                                                                  tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg              >  2:2468360/1‑100 (MQ=255)
                                                                                  tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg              <  2:293858/100‑1 (MQ=255)
                                                                                   gCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCgg             <  1:2745008/100‑1 (MQ=255)
                                                                                    cGGGCCGGCCAGATCGGCAGCAGTAATGCGATCGCGTTAGTACCGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGa            <  2:4128697/100‑1 (MQ=255)
                                                                                     gggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGAt           <  1:3228644/100‑1 (MQ=255)
                                                                                       gTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTc         >  1:708664/1‑100 (MQ=255)
                                                                                          ggCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAcc      >  1:3197366/1‑100 (MQ=255)
                                                                                          ggCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAcc      >  1:1123035/1‑100 (MQ=255)
                                                                                           gCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCg     <  1:52596/100‑1 (MQ=255)
                                                                                            ccAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGc    >  1:832627/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCC  >  NC_000913/1826934‑1827127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: