Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,841,079 C→A 36.2% A109S (GCG→TCG)  ynjF ← CDP‑alcohol phosphatidyltransferase family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,841,0790CA36.2% 172.8 / 165.3 155A109S (GCG→TCG) ynjFCDP‑alcohol phosphatidyltransferase family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (56/43);  new base A (30/26);  total (86/69)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 161 total aligned reads displayed.

CGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAGCT  >  NC_000913/1840980‑1841177
                                                                                                   |                                                                                                  
cGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAgcgc                                                                                                    >  2:2011437/1‑100 (MQ=255)
 ggggTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCa                                                                                                   >  2:1277109/1‑100 (MQ=255)
   ggTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCAtt                                                                                                 >  2:3438603/1‑100 (MQ=255)
   ggTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAAtt                                                                                                 >  1:635501/1‑100 (MQ=255)
    gTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCAttt                                                                                                <  1:303874/100‑1 (MQ=255)
    gTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAAttt                                                                                                <  1:4139855/100‑1 (MQ=255)
     ntATCAATCTGATGTTTTGCTGCCAGTGCAGCATAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCAtttt                                                                                               >  1:2819998/2‑100 (MQ=255)
      tATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTg                                                                                              >  2:175120/1‑100 (MQ=255)
      tATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTg                                                                                              <  2:3347659/100‑1 (MQ=255)
      tATCAAGCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTg                                                                                              <  2:466097/100‑1 (MQ=255)
       aTCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGc                                                                                             >  1:2454387/1‑100 (MQ=255)
       aTCAATCTGATGTTTTGCTGCAAGTGCAGCAAAGGCGAGAAAACCGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGc                                                                                             <  1:567425/100‑1 (MQ=255)
        tCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCTGTGCCGATAAACGCAAACAAAAACCAGCCGCCTGCCAGCGCATTTTGCt                                                                                            >  1:2905121/1‑100 (MQ=255)
        tCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCACATTTTGCt                                                                                            >  1:2172967/1‑100 (MQ=255)
          aaTCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTcc                                                                                          >  2:466301/1‑100 (MQ=255)
            tCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTACTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCgg                                                                                        <  2:1703304/100‑1 (MQ=255)
            tCTGATGTTTTGCTCCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCgg                                                                                        <  1:2907796/100‑1 (MQ=255)
             cTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGt                                                                                       >  1:1995392/1‑100 (MQ=255)
             cTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGt                                                                                       <  1:4108351/100‑1 (MQ=255)
               gATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTgc                                                                                     <  2:3172627/100‑1 (MQ=255)
                aTGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTgca                                                                                    <  1:2215981/100‑1 (MQ=255)
                  gTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTgcagc                                                                                  >  2:193301/1‑100 (MQ=255)
                    tttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCaa                                                                                >  2:1586519/1‑100 (MQ=255)
                    tttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCaa                                                                                >  2:1862317/1‑100 (MQ=255)
                     ttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCCGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAg                                                                               <  2:3004647/100‑1 (MQ=255)
                      tgctgcCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGa                                                                              >  2:1073776/1‑100 (MQ=255)
                       gctgcCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGaa                                                                             >  2:2382272/1‑100 (MQ=255)
                       gctgcCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGaa                                                                             >  1:1493226/1‑100 (MQ=255)
                        ctgcCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAAt                                                                            >  1:3917351/1‑100 (MQ=255)
                        ctgcCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAAt                                                                            <  1:2171368/100‑1 (MQ=255)
                        ccgcCAGTGCAGCGAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAAt                                                                            >  2:2901929/3‑100 (MQ=255)
                         tgcCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATACACGCAAAAAACAACCAGCCGCCCGGCAGCGCATTTCGATCCGGTGAAGCAAGa                                                                              >  1:3972995/1‑97 (MQ=255)
                          gcCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATaa                                                                          >  2:204298/1‑100 (MQ=255)
                          gcCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATaa                                                                          >  2:3942853/1‑100 (MQ=255)
                           cactggcagcaAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATaaa                                                                         >  2:4130866/6‑100 (MQ=255)
                            cAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAg                                                                        <  1:2059088/100‑1 (MQ=255)
                            cAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAg                                                                        <  1:4260075/100‑1 (MQ=255)
                             aGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAATCGCAAACAACAACCAGCCGCCGGCCAGCTCATTTTGCGCCGGTGCAGCAAGAATCAAGc                                                                       <  1:2609791/100‑1 (MQ=255)
                             aGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGc                                                                       <  2:3089697/100‑1 (MQ=255)
                             aGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGc                                                                       <  2:3534004/100‑1 (MQ=255)
                              gTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGcc                                                                      <  2:1027122/100‑1 (MQ=255)
                                 cagcaAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCaaa                                                                   >  1:2020454/1‑100 (MQ=255)
                                   gcaAAGGCGAGAAAACTGCTGTCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACg                                                                 >  1:484746/1‑100 (MQ=255)
                                   gcaAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACg                                                                 >  2:2558072/1‑100 (MQ=255)
                                    caAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACgg                                                                >  2:3958315/1‑100 (MQ=255)
                                        ggCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCAcc                                                            <  1:3447604/100‑1 (MQ=255)
                                        gatctgaAAACTGCTGGCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCAcc                                                            <  1:2491171/95‑1 (MQ=255)
                                         gCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCAcc                                                            <  1:3889742/99‑1 (MQ=255)
                                          cGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAg                                                          >  2:3870164/1‑100 (MQ=255)
                                          cGAGAAAACTGCTGCCCGTACCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCActa                                                           >  1:2344080/1‑97 (MQ=255)
                                           gagaAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCCTTTTGCTCCGCTGCAGCAAGAATAAAGCCAAACGGCACCAgc                                                         >  2:4115374/1‑100 (MQ=255)
                                            agaAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAgcg                                                        <  1:3197674/100‑1 (MQ=255)
                                            agaAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAgcg                                                        <  1:3529577/100‑1 (MQ=255)
                                             gaAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAgcgc                                                       >  2:4002949/1‑100 (MQ=255)
                                             gaAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAgcgc                                                       >  2:1101608/1‑100 (MQ=255)
                                             gaAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAgatc                                                       >  2:2491178/1‑97 (MQ=255)
                                                aaCTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGcgcg                                                      >  2:2155931/1‑98 (MQ=255)
                                                aaCTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTa                                                    >  2:775405/1‑100 (MQ=255)
                                                aaCTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTa                                                    <  1:937759/100‑1 (MQ=255)
                                                  ctgctgCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTaaa                                                  >  2:3748302/1‑100 (MQ=255)
                                                  ctgctgCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATCTTGCACCGGGGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTaaa                                                  >  1:2762137/1‑100 (MQ=255)
                                                    gctgcCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAag                                                <  2:3033658/100‑1 (MQ=255)
                                                      tgcCCGTGTCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAaga                                               <  1:2378059/99‑1 (MQ=255)
                                                      tgcCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAagag                                              <  2:2771745/100‑1 (MQ=255)
                                                         ccGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGACTAAAGCCCAATGGCAACAACCCGCAAGAGAGaaa                                           >  1:3719313/1‑100 (MQ=255)
                                                         ccGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGaaa                                           <  1:277637/100‑1 (MQ=255)
                                                          cGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGaaaa                                          >  2:2758723/1‑100 (MQ=255)
                                                            tGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATc                                        >  1:3856072/1‑100 (MQ=255)
                                                             gCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCg                                       >  2:1264492/1‑100 (MQ=255)
                                                              ccGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCCCCAGCGCGTAAAAGAGAAAATCga                                      >  2:1014634/1‑100 (MQ=255)
                                                               cGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCgag                                     <  2:1672459/100‑1 (MQ=255)
                                                                gATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCgaga                                    <  2:3856089/100‑1 (MQ=255)
                                                                gATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCACCGGAGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCgaga                                    <  1:540751/100‑1 (MQ=255)
                                                                    aaCGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGaaa                                >  1:3912248/1‑100 (MQ=255)
                                                                    aaCGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAAACGAGAGaaa                                >  1:1660704/1‑100 (MQ=255)
                                                                     aCGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAat                               >  1:2076427/1‑100 (MQ=255)
                                                                      cGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAata                              <  1:1903924/100‑1 (MQ=255)
                                                                      cGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAata                              <  2:319516/100‑1 (MQ=255)
                                                                       gCACACAACGCCCAGCCGCCTGCCAGGCCATACTGCTCCGGTGCACCAAAAATAAAGCGGAACGGCACTATCGCGCAAAAGAGAAAACCGAGAGaac                                <  1:3777735/97‑2 (MQ=255)
                                                                          aacaacaacCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCaa                          <  2:819006/100‑1 (MQ=255)
                                                                          aacaacaacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCaa                          <  1:1101605/100‑1 (MQ=255)
                                                                          aacaacaacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCaa                          <  2:285134/100‑1 (MQ=255)
                                                                           acaacaacCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAg                         >  2:2861422/1‑100 (MQ=255)
                                                                            caacaacCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGa                        >  1:139031/1‑100 (MQ=255)
                                                                              acaacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGaaa                      >  2:314830/1‑100 (MQ=255)
                                                                              acaacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGaaa                      >  2:312919/1‑100 (MQ=255)
                                                                              acaacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGaaa                      >  2:3480761/1‑100 (MQ=255)
                                                                              acaacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGaaa                      >  2:427320/1‑100 (MQ=255)
                                                                                    aGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAgccgcc                <  2:1503199/100‑1 (MQ=255)
                                                                                        gccTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCcgcg            <  2:1083736/100‑1 (MQ=255)
                                                                                          cTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTc          <  2:903768/100‑1 (MQ=255)
                                                                                           tGCCAGCGCATTTTGCTCCGGTGCAGAAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCg         >  2:2606710/1‑100 (MQ=255)
                                                                                           tGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAAAATCAAGAAAGCCGCCCGCGTCg         >  1:3293742/1‑100 (MQ=255)
                                                                                             ccAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGt       >  1:1285454/1‑100 (MQ=255)
                                                                                             ccAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGt       >  1:833519/1‑100 (MQ=255)
                                                                                              cggcgaATTTTGCTCCGGTGCAGCAAGATTAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAATAAAGCCGCCCGCCTCg         >  1:193711/3‑97 (MQ=255)
                                                                                               aGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTa      <  2:3618106/99‑1 (MQ=255)
                                                                                                 cgcATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAGc   <  1:852373/100‑1 (MQ=255)
                                                                                                 cgcATTTTGCTCCGGTGCAACAAGAATACAGCCAAACGACACCAGCGCGTAAAAGCGAAAATCGAGAGAAATATCAAGAAAGCCGCCCTCGTCGGTaatg   <  1:1848787/100‑3 (MQ=255)
                                                                                                  gcATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAGct  >  1:345146/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
CGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAGCT  >  NC_000913/1840980‑1841177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑