Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 252,594 G→C 15.4% W98C (TGG→TGC yafO → mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913252,5940GC15.4% 479.9 / 76.1 177W98C (TGG→TGCyafOmRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (77/72);  new base C (13/14);  total (91/86)
Fisher's exact test for biased strand distribution p-value = 8.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01

Only 100 of 184 total aligned reads displayed.

TGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGT  >  NC_000913/252495‑252693
                                                                                                   |                                                                                                   
tGAGAATAATCCATTTCCGCCACAGTTGCGTCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATgg                                                                                                     <  1:3450696/100‑1 (MQ=255)
tGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATgg                                                                                                     >  1:2173978/1‑100 (MQ=255)
 gagaATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGt                                                                                                    <  1:2009660/100‑1 (MQ=255)
  agaATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGtt                                                                                                   >  2:521742/1‑100 (MQ=255)
  agaATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGtt                                                                                                   >  1:4125308/1‑100 (MQ=255)
   gaATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTg                                                                                                  <  1:2679769/100‑1 (MQ=255)
     ataatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCt                                                                                                <  2:2496452/100‑1 (MQ=255)
       aatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCa                                                                                              >  2:2170405/1‑100 (MQ=255)
       aatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCa                                                                                              <  2:2046796/100‑1 (MQ=255)
        atCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCAt                                                                                             >  2:3910753/1‑100 (MQ=255)
        atCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAt                                                                                             <  1:3715763/100‑1 (MQ=255)
        atCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAt                                                                                             <  2:3721340/100‑1 (MQ=255)
         tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                            <  2:2257326/100‑2 (MQ=255)
         tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                            <  1:4194502/100‑2 (MQ=255)
         tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                            <  2:1072157/100‑2 (MQ=255)
         tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGATTGATGAGCAACCATGCTTGCTCAtc                                                                                            <  2:2599608/100‑2 (MQ=255)
          ccATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTg                                                                                           >  2:2539857/1‑100 (MQ=255)
          ccATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTg                                                                                           <  2:3766431/100‑1 (MQ=255)
          ccATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTg                                                                                           >  2:3895925/1‑100 (MQ=255)
           cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTg                                                                                           >  1:2906472/1‑99 (MQ=255)
           cATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGc                                                                                          <  2:232675/100‑1 (MQ=255)
            aTTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGcc                                                                                         >  2:1186829/1‑100 (MQ=255)
            aTTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGc                                                                                          >  1:906157/1‑99 (MQ=255)
            aTTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtcaaa                                                                                         <  2:3526003/100‑5 (MQ=39)
                ccGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGGTTGATGGGCAAGCATGGTTGCTCATTGCCATTc                                                                                     <  2:3618030/100‑1 (MQ=255)
                ccGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTc                                                                                     <  1:2875746/100‑1 (MQ=255)
                  gCCTCAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTg                                                                                   >  1:2087982/1‑100 (MQ=255)
                     acaGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGaaa                                                                                <  2:1447058/100‑1 (MQ=255)
                     acaGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGaaa                                                                                <  2:2946773/100‑1 (MQ=255)
                      caGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAAc                                                                               <  1:187213/100‑1 (MQ=255)
                       aGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCGTTCTGAAAcc                                                                              <  1:392072/100‑1 (MQ=255)
                         ttGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTg                                                                            <  2:1898223/100‑1 (MQ=255)
                           gcgcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGaa                                                                          <  1:2535988/100‑1 (MQ=255)
                            cgcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAAc                                                                         >  1:2163995/1‑100 (MQ=255)
                             gcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAAcc                                                                        <  1:475813/100‑1 (MQ=255)
                             gcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAAcc                                                                        >  2:25695/1‑100 (MQ=255)
                              ncAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCt                                                                       >  1:3248484/2‑100 (MQ=255)
                              ccAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGACATTCTGAAACCTGaa                                                                          >  1:3283366/1‑97 (MQ=255)
                               cAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTc                                                                      >  1:3205695/1‑100 (MQ=255)
                                aaTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCa                                                                     <  2:3288688/100‑1 (MQ=255)
                                aaTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCa                                                                     <  2:4113341/100‑1 (MQ=255)
                                 aTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCAt                                                                    <  2:4088789/100‑1 (MQ=255)
                                  ttCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATa                                                                   <  2:3982436/100‑1 (MQ=255)
                                  ttCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATa                                                                   >  2:778863/1‑100 (MQ=255)
                                    cagcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATaaa                                                                 >  1:4051338/1‑100 (MQ=255)
                                      gcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACt                                                               <  2:3337300/100‑1 (MQ=255)
                                           aCGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGTCATTCTGAAACCTGAACCTCATAAACTGGCTc                                                          <  2:4051358/100‑1 (MQ=255)
                                             gAATGACGAAGCGCATTTGGTCTATGGTCAGGGGGCGTTTGATGAGCAAGCATGGTGGCTCATTGCCATGATGAAACCTGAACCTCATCAACTGGCTCga                                                        >  1:1412469/1‑100 (MQ=255)
                                              aaTGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgag                                                       >  2:1986473/1‑100 (MQ=255)
                                                 gACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATa                                                    <  2:469986/100‑1 (MQ=255)
                                                  aCGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaa                                                   >  1:518167/1‑100 (MQ=255)
                                                   cGAAGCGCATTTGGTATATTGTTAGTGTTCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaac                                                  >  1:169923/1‑100 (MQ=255)
                                                   cGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaac                                                  <  2:1088444/100‑1 (MQ=255)
                                                   cGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaac                                                  <  2:1002657/100‑1 (MQ=255)
                                                    gAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaaca                                                 >  2:1511570/1‑100 (MQ=255)
                                                    gAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaaca                                                 >  1:3683761/1‑100 (MQ=255)
                                                      aGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaacaac                                               <  2:735258/100‑1 (MQ=255)
                                                      aGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaacaac                                               <  2:3583615/100‑1 (MQ=255)
                                                      aGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaacaac                                               >  1:1004914/1‑100 (MQ=255)
                                                      aGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaacaac                                               <  2:977053/100‑1 (MQ=255)
                                                      aGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaacaac                                               >  1:375997/1‑100 (MQ=255)
                                                         gcATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCaa                                            >  2:1603264/1‑100 (MQ=255)
                                                           aTTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAAt                                          >  2:3926802/1‑100 (MQ=255)
                                                                gTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATa                                     >  1:2032423/1‑100 (MQ=255)
                                                                  atatTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATaaa                                   <  2:3798901/100‑1 (MQ=255)
                                                                    atTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAAt                                 <  1:937617/100‑1 (MQ=255)
                                                                    atTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAAt                                 >  2:4161437/1‑100 (MQ=255)
                                                                     ttGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAAtt                                <  2:762115/100‑1 (MQ=255)
                                                                      tGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTg                               <  2:931043/100‑1 (MQ=255)
                                                                      tGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCGCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAGCAACCACATGCATAAAATTg                               >  2:694503/1‑100 (MQ=255)
                                                                       gTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTgg                              <  2:1919832/100‑1 (MQ=255)
                                                                       gTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTgg                              >  2:2665477/1‑100 (MQ=255)
                                                                       gTCAGGGGGCGATTGATGAGCACGCATGGTTGCTCATTGCCATAATGAAACCTTAACCTCTTAAACTGGCTAGAGATAACAAACAAATGCCAAAAATTgg                              >  1:179309/1‑100 (MQ=255)
                                                                             gggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAAt                        <  1:3576625/100‑1 (MQ=255)
                                                                              ggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAACCCTGAACCTCATAAACTGGCTCGAGATAACACCCAAATGTCTAAAAATGGGAAAAt                        >  1:29900/1‑99 (MQ=255)
                                                                              ggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATg                       <  1:818351/100‑1 (MQ=255)
                                                                              ggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATg                       >  1:3138190/1‑100 (MQ=255)
                                                                               gCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATgg                      >  1:1583932/1‑100 (MQ=255)
                                                                                cGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGc                     <  1:1181677/100‑1 (MQ=255)
                                                                                atggcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGc                     >  2:672787/7‑100 (MQ=38)
                                                                                 gTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCa                    >  2:2426369/1‑100 (MQ=255)
                                                                                  tttGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAg                   <  2:508331/100‑1 (MQ=255)
                                                                                  ggcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAg                   >  2:2723397/5‑100 (MQ=39)
                                                                                   gcaatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGGAAATGGCAGa                  >  2:4118112/4‑100 (MQ=255)
                                                                                   gcaatgaGCAACCATGCTTGCTCATTGCAATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCa                    >  1:2781938/4‑98 (MQ=39)
                                                                                    caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa                 >  2:136141/3‑100 (MQ=255)
                                                                                    caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa                 >  1:2622576/3‑100 (MQ=255)
                                                                                    caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa                 >  1:3212718/3‑100 (MQ=255)
                                                                                     gatggGCAAGCATGGATGCTCATTGGTATCCTAAAAATTGACCATCACAAATTCGGTCGACATAAAAACCTGATGCAGAAAATTGGGAAAATAGc                     <  1:2436285/95‑1 (MQ=255)
                                                                                     gatgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAg                >  1:4245020/1‑100 (MQ=255)
                                                                                     gatgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAg                <  1:2776263/100‑1 (MQ=255)
                                                                                          gcaagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGttt           >  2:3124386/1‑100 (MQ=255)
                                                                                            aagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCg         <  1:1093032/100‑1 (MQ=255)
                                                                                              gcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCa       <  1:3062764/100‑1 (MQ=255)
                                                                                                aTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATg     <  1:722042/100‑1 (MQ=255)
                                                                                                 tGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGc    >  2:1344724/1‑100 (MQ=255)
                                                                                                 tGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATCCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCAGGc    >  2:1718865/1‑100 (MQ=255)
                                                                                                  ggTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCg   <  2:1345840/100‑1 (MQ=255)
                                                                                                  ggTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCg   <  1:4136445/100‑1 (MQ=255)
                                                                                                   gTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGt  >  1:1009990/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGT  >  NC_000913/252495‑252693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: