Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,870,983 C→T 36.9% A200V (GCG→GTG)  yeaI → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,870,9830CT36.9% 125.3 / 123.2 133A200V (GCG→GTG) yeaIputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (44/39);  new base T (21/28);  total (66/67)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

AACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCA  >  NC_000913/1870884‑1871082
                                                                                                   |                                                                                                   
aaCTCAGTTTTATTTGTTTAACCTCGCTGTCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGgc                                                                                                     <  2:850194/100‑1 (MQ=255)
aaCTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGgc                                                                                                     <  2:251085/100‑1 (MQ=255)
 aCTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGgc                                                                                                     >  1:1253703/1‑99 (MQ=255)
  cTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGGATCCTGTTGCTGgtgc                                                                                                   >  1:2491733/1‑100 (MQ=255)
   tCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCt                                                                                                  <  1:3579400/100‑1 (MQ=255)
    cAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTg                                                                                                 >  2:2590698/1‑100 (MQ=255)
    cAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTg                                                                                                 <  1:2961959/100‑1 (MQ=255)
     aGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGa                                                                                                >  1:3654102/1‑100 (MQ=255)
     aGTTTTATTTGTTCAACCTCGCTGGCGCTCTTTTGTTAGGGAAAAGACAACATCCTTGACGACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGa                                                                                                >  1:2638850/1‑100 (MQ=255)
       ttttATTTTTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATc                                                                                              <  1:3864148/100‑1 (MQ=255)
       ttttATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATc                                                                                              <  2:3914360/100‑1 (MQ=255)
       ttttATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATc                                                                                              >  2:1689088/1‑100 (MQ=255)
        ttaaTTTGTTTAACCTCGCTAGCGCTATTTTGTTATGGAAAAGACTACATCCTTGAAAAAAAAAAAAAAAAAACGGGAATCCTGTTGCTGGCGCTGATc                                                                                              <  1:887178/96‑1 (MQ=255)
          tATTTGTTTAACCTCGCTGGCTCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCt                                                                                           >  2:2949908/1‑100 (MQ=255)
           aTTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCtt                                                                                          >  2:16310/1‑100 (MQ=255)
                tttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTa                                                                                     <  1:1666450/100‑1 (MQ=255)
                 ttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAg                                                                                    <  2:4033437/100‑1 (MQ=255)
                  tAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTACTGATCCCTTTTTTAGt                                                                                   >  1:387005/1‑100 (MQ=255)
                  tAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGt                                                                                   >  2:1021129/1‑100 (MQ=255)
                  tAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGt                                                                                   <  2:3149212/100‑1 (MQ=255)
                   aaCCTCGCTGGCGCTCTTTTGTTATCGAAAAGACAACATCCTTGAAAACAATACGAAATAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGt                                                                                   <  1:1051810/99‑1 (MQ=255)
                   aaCCTCGCTCGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGtt                                                                                  <  1:4148867/100‑1 (MQ=255)
                   aaCCTATCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGATAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGtt                                                                                  <  1:4045992/100‑1 (MQ=255)
                    aCCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGttt                                                                                 <  2:342127/100‑1 (MQ=255)
                     ccTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGtttt                                                                                >  2:3620664/1‑100 (MQ=255)
                            ggCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCtt                                                                         >  2:2156840/1‑100 (MQ=255)
                              cgcTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCTCCtttt                                                                       >  1:2187992/1‑98 (MQ=255)
                              cgcTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCt                                                                       >  1:1922940/1‑100 (MQ=255)
                                  ctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGca                                                                   >  2:2492120/1‑100 (MQ=255)
                                  ctTTTGTTATCGAAAAGACAATATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGca                                                                   <  1:4039887/100‑1 (MQ=255)
                                   ttttGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcac                                                                  <  2:1967313/100‑1 (MQ=255)
                                    tttGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcaca                                                                 >  2:371973/1‑100 (MQ=255)
                                     ttGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcacac                                                                <  1:2915815/100‑1 (MQ=255)
                                      tGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcacaca                                                               >  1:805568/1‑100 (MQ=255)
                                      tGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTAGTTTTTCCCCTTCTGGCACACaa                                                              >  2:1937814/1‑100 (MQ=255)
                                       gTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACaa                                                              >  2:2753023/1‑100 (MQ=255)
                                       gTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACaa                                                              >  2:2092896/1‑100 (MQ=255)
                                         naTGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATc                                                            >  2:2524461/2‑100 (MQ=255)
                                          aTGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCt                                                           <  2:2395267/100‑1 (MQ=255)
                                           tGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTg                                                          <  2:1436457/100‑1 (MQ=255)
                                           tGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTg                                                          >  2:1515396/1‑100 (MQ=255)
                                            ggAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGa                                                         >  2:4268669/1‑100 (MQ=255)
                                            ggAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTCTTTCCCCTTCTGGCACACAATCTGa                                                         >  2:2154828/1‑100 (MQ=255)
                                            ggAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTGTTAGTTTTTCCCCTTCTGGCACACAATCTGa                                                         >  1:925731/1‑100 (MQ=255)
                                             gAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAg                                                        >  1:2297496/1‑100 (MQ=255)
                                             gAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAg                                                        >  1:3215378/1‑100 (MQ=255)
                                             gAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAg                                                        <  2:3395221/100‑1 (MQ=255)
                                             gAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGAGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAg                                                        <  1:1198400/100‑1 (MQ=255)
                                              aaaaGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTTGCACACAATCTGAGc                                                       <  2:3187687/100‑1 (MQ=255)
                                              aaaaGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                                       <  2:2317802/100‑1 (MQ=255)
                                              aaaaGACAACATCCTTGACAAAAAAAAAAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAg                                                        <  1:3634661/99‑1 (MQ=255)
                                               aaaGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCa                                                      >  1:2830390/1‑100 (MQ=255)
                                                aaGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAg                                                     <  2:3748721/100‑1 (MQ=255)
                                                 aGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGt                                                    <  1:3167810/100‑1 (MQ=255)
                                                    caacaTCCTTGACAACAAAAAGAAAAAAAAGGGAATCCTGTTGCTGGAGCTGATTCCTTTTTTAGTTTTTCACCTTCTGGTACACAATCTGAGCAGTtat                                                 >  1:723888/1‑100 (MQ=255)
                                                     aacaTCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTtata                                                <  2:1561696/100‑1 (MQ=255)
                                                      acaTCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATaa                                               <  2:354974/100‑1 (MQ=255)
                                                       caTCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAAc                                              >  2:2954244/1‑100 (MQ=255)
                                                       caTCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAAc                                              >  2:3843917/1‑100 (MQ=255)
                                                           cTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTg                                          >  2:104631/1‑100 (MQ=255)
                                                           cTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTg                                          <  1:3445562/100‑1 (MQ=255)
                                                            ttGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGa                                         >  2:736442/1‑100 (MQ=255)
                                                              gACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACt                                       >  1:750424/1‑100 (MQ=255)
                                                                ccacaaTAAGAAAGAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTAt                                     >  2:2905007/3‑100 (MQ=255)
                                                                  acaaTAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTc                                   >  2:1082768/1‑100 (MQ=255)
                                                                  acaaTAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTc                                   >  2:3296249/1‑100 (MQ=255)
                                                                    aaaaaaaaaaaaaCGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCtt                                 <  1:3101366/94‑1 (MQ=255)
                                                                     aTAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCttt                                <  1:496852/100‑1 (MQ=255)
                                                                     aTAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCttt                                <  1:245145/100‑1 (MQ=255)
                                                                      tAAGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTg                               <  1:3253564/100‑1 (MQ=255)
                                                                       aaGAAAAAAACGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGt                              <  2:364936/100‑1 (MQ=255)
                                                                        aGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTa                             <  2:490853/100‑1 (MQ=255)
                                                                         gAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTCTTTAt                            >  1:311399/1‑100 (MQ=255)
                                                                          aaaaaaaCGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTTTTTTTTTTTTTTCTGGCACACAATCTGAGCAGTTATAATGTTGACTATTTTTTGTAt                            >  1:599992/1‑99 (MQ=255)
                                                                          aaaaaaaCGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg                           >  1:2215820/1‑100 (MQ=255)
                                                                          aaaaaaaCGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg                           <  1:3659056/100‑1 (MQ=255)
                                                                           aaaaaaCGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATgt                          >  2:2446952/1‑100 (MQ=255)
                                                                           aaaaaaCAGGAATCCTGTTGCTGCCGCTGATCCCTTTTTTAGTTTCTCCCCTACTGGCACACAATCTGAGCAGTTATAACGCTTACTATTCTTTTTATg                           <  1:2859585/99‑1 (MQ=255)
                                                                             aaaaCGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATgtcg                        >  1:1702514/1‑100 (MQ=255)
                                                                               aaCGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATgtcgtc                      <  2:2013258/100‑1 (MQ=255)
                                                                               aaCGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATgtcgtc                      <  1:1848309/100‑1 (MQ=255)
                                                                                aCGGGAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGtcgtcg                     <  1:4109956/100‑1 (MQ=255)
                                                                                aCGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGtcgtcg                     <  2:3363483/100‑1 (MQ=255)
                                                                                   ggAATCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGAtt                  <  2:1095465/100‑1 (MQ=255)
                                                                                   ggAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGAtt                  <  2:3095986/100‑1 (MQ=255)
                                                                                    gAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTa                 <  1:4260693/100‑1 (MQ=255)
                                                                                    gAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTa                 >  1:1099843/1‑100 (MQ=255)
                                                                                     aaTCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTAc                <  1:2569929/100‑1 (MQ=255)
                                                                                     aaTCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTAc                <  2:3078689/100‑1 (MQ=255)
                                                                                     aaTCCTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTAc                <  2:2204056/100‑1 (MQ=255)
                                                                                      aTCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACt               <  2:3975713/100‑1 (MQ=255)
                                                                                         cTGTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTc            >  1:2353611/1‑100 (MQ=255)
                                                                                           gTTGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCg          >  2:2223583/1‑100 (MQ=255)
                                                                                           gTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCg          <  1:3005330/100‑1 (MQ=255)
                                                                                           gTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCg          <  2:453294/100‑1 (MQ=255)
                                                                                             tGCTGGTGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGa        >  1:903243/1‑100 (MQ=255)
                                                                                                tGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACaa     >  1:2177780/1‑100 (MQ=255)
                                                                                                 ggCGCTGATCCCTTTTTTAGTTTTTGTTCTTCTGGCACAGAAACTCAGCAATTATAACGCTGACTATTCTTTGTATGTTGTCGCTTACTCTCCg          >  1:485753/1‑94 (MQ=255)
                                                                                                 ggCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAAc    <  2:4056453/100‑1 (MQ=255)
                                                                                                   cgcTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCa  <  1:392945/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCA  >  NC_000913/1870884‑1871082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: