Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,913,273 T→C 41.3% N515S (AAC→AGC)  prc ← carboxy‑terminal protease for penicillin‑binding protein 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,913,2730TC41.3% 69.4 / 184.4 156N515S (AAC→AGC) prccarboxy‑terminal protease for penicillin‑binding protein 3
Reads supporting (aligned to +/- strand):  ref base T (42/49);  new base C (28/36);  total (71/85)
Fisher's exact test for biased strand distribution p-value = 8.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01

Only 100 of 161 total aligned reads displayed.

AGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTAT  >  NC_000913/1913174‑1913371
                                                                                                   |                                                                                                  
aGCGCGTTATCTTCGAATTTCTCACCGGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGt                                                                                                    <  1:2722140/100‑1 (MQ=255)
aGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGt                                                                                                    <  2:726331/100‑1 (MQ=255)
 gcgcGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATATCTGGCTTTACGCCTTTACGTTGCGTACTACCGCCGtt                                                                                                   >  1:946479/1‑100 (MQ=255)
  cgcgTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTa                                                                                                  <  2:1439651/100‑1 (MQ=255)
  cgcgTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTa                                                                                                  <  1:3823024/100‑1 (MQ=255)
  cgcgTTATCTTCGAATTTCTCACCCGTTTCCGTTTCATCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTa                                                                                                  >  2:3013912/1‑100 (MQ=255)
    cgTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTAATGCCGCCGCTaa                                                                                                 >  1:3424888/1‑99 (MQ=255)
      ttATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAAcgc                                                                                              <  1:1058223/100‑1 (MQ=255)
      ttATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAAcgc                                                                                              <  1:770297/100‑1 (MQ=255)
       tATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAAcgcg                                                                                             <  1:1746460/100‑1 (MQ=255)
         tCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGAt                                                                                           <  1:2972430/100‑1 (MQ=255)
         tCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGAt                                                                                           <  1:1371670/100‑1 (MQ=255)
           ttCGAATTTCTCACTCGTTTGCGTTTCTTGATTACCCGTCGGCATGATGATGTCTGGCGTTAGGCCTTTCCGTTGCGTACTGCCGCCCGTAACGCGATat                                                                                         >  1:848917/1‑99 (MQ=255)
           ttCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAg                                                                                         >  2:1234136/1‑100 (MQ=255)
           ttCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAg                                                                                         >  2:3332453/1‑100 (MQ=255)
           ttCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAg                                                                                         >  1:3173224/1‑100 (MQ=255)
              gAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAAt                                                                                      >  2:1372463/1‑100 (MQ=255)
               aaTTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAAtt                                                                                     >  2:4225901/1‑100 (MQ=255)
                 tttCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTc                                                                                   <  2:1349321/100‑1 (MQ=255)
                  ttCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCt                                                                                  <  1:120307/100‑1 (MQ=255)
                   tctcACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTg                                                                                 <  2:3587879/100‑1 (MQ=255)
                      caaccgttacaggttATTCATTACCCGTCGGCATGATGCTTTCTGGCGTTACGCCTTTACGTTGCGTACTGAGGCCGTTTCCGCGATAGAATTTCTGGa                                                                               >  1:1766553/4‑99 (MQ=255)
                      cACCCGTTTCCGTTTCTTCATTACCCGTCGGCCTGATGATGTCTGGCGTTACTCCTTTACGTTGCGTACTGCCGCCGTTACCGCGCTAGAATTTCTGGAt                                                                              >  1:1662762/1‑100 (MQ=255)
                      cACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGAt                                                                              >  1:1497771/1‑100 (MQ=255)
                      cACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGAt                                                                              <  1:2481356/100‑1 (MQ=255)
                        cccGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCg                                                                            >  2:2832283/1‑100 (MQ=255)
                        cccGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCg                                                                            <  2:719331/100‑1 (MQ=255)
                        cccGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCg                                                                            <  1:354516/100‑1 (MQ=255)
                        cccGTTTCCGTTTCTGCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCg                                                                            <  2:4203218/100‑1 (MQ=255)
                          cgtttccgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCgtg                                                                          >  2:1999451/1‑100 (MQ=255)
                           gtttccgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCgtgt                                                                         <  1:2216840/100‑1 (MQ=255)
                            tttccgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTa                                                                        >  2:2747194/1‑100 (MQ=255)
                             ttccgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTAc                                                                       <  1:3936747/100‑1 (MQ=255)
                             tgccgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGACAGAATTTCTGGATCGTGTAc                                                                       <  1:2599397/98‑1 (MQ=255)
                              tccttttcTTCATTACCGGTCGGCATGATGATGTCTCGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACt                                                                      <  1:4005999/100‑1 (MQ=255)
                              tccgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACt                                                                      >  1:4186316/1‑100 (MQ=255)
                                cgtttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGc                                                                    <  2:2487856/100‑1 (MQ=255)
                                cgtttcTTCACTGTTTGTCGACATGGGCATGTTTGGTGTTACGCCCTTAGGGGGCGCACTGGCGGCGTTAGCGCTATAGGATTTCTGGATCGTGTACt                                                                      <  1:1169082/98‑1 (MQ=255)
                                  tttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGcac                                                                  <  1:1644126/100‑1 (MQ=255)
                                  tttcTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACCCCTTTACGTTGCGTACTGCCTCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGcac                                                                  <  2:3726369/100‑1 (MQ=255)
                                   ttcttcATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGcaca                                                                 <  1:1833958/100‑1 (MQ=255)
                                    tcttcATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAg                                                                <  2:2361417/100‑1 (MQ=255)
                                     cttcATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGa                                                               >  2:3519748/1‑100 (MQ=255)
                                     cttcATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGa                                                               >  1:3429763/1‑100 (MQ=255)
                                      ttcATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGaa                                                              >  2:403376/1‑100 (MQ=255)
                                       tcATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAAc                                                             <  1:709310/100‑1 (MQ=255)
                                       tcATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAAc                                                             <  2:1721212/100‑1 (MQ=255)
                                        cATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAAcc                                                            >  1:1571662/1‑100 (MQ=255)
                                        cATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAAcc                                                            >  1:2808116/1‑100 (MQ=255)
                                         aTTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAAccc                                                           <  1:3287112/100‑1 (MQ=255)
                                         aTTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAAccc                                                           <  1:308107/100‑1 (MQ=255)
                                         aTTACCCGTCGGCATGATGATGTCTGGAGTTAAGCCTTTACGTTGCGTACTGCAGCCGCTAAGGCGATAGTATTTCTGGCTCGTGTGCTGCACAGAAcc                                                            >  2:1419449/1‑99 (MQ=255)
                                             cccGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAgcg                                                       <  2:481731/100‑1 (MQ=255)
                                                gTCGGCATGATGCTGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTg                                                    >  2:2812626/1‑100 (MQ=255)
                                                 tCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTgg                                                   <  2:417865/100‑1 (MQ=255)
                                                 tCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTgg                                                   <  2:2012931/100‑1 (MQ=255)
                                                  cGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGc                                                  <  2:1910525/100‑1 (MQ=255)
                                                   tgCATGATGCTGTCTGGCTTTACGCCTTTTCGTTGCGTACTGCCGCCGTTCACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGc                                                  <  1:2271756/98‑1 (MQ=255)
                                                   ggCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGcc                                                 <  1:423214/100‑1 (MQ=255)
                                                    gCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCa                                                >  2:360393/1‑100 (MQ=255)
                                                       tgatgatgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTc                                             >  2:2699649/1‑100 (MQ=255)
                                                        gatgatgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCa                                            <  1:1287189/100‑1 (MQ=255)
                                                           gatgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGa                                         <  1:1959524/100‑1 (MQ=255)
                                                           gatgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGa                                         <  2:525058/100‑1 (MQ=255)
                                                            atgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGAc                                        >  1:1004634/1‑100 (MQ=255)
                                                             tgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACg                                       >  2:3697976/1‑100 (MQ=255)
                                                             tgTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACg                                       >  2:2576685/1‑100 (MQ=255)
                                                               tCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTa                                     >  1:2067317/1‑100 (MQ=255)
                                                               tCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTa                                     <  2:3956095/100‑1 (MQ=255)
                                                                cTGGCGTTACACCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTaa                                    >  2:3117531/1‑100 (MQ=255)
                                                                 tGGCGTTACTCCTTTACGTTGCGTACTGCCCCCGCTAACGCAATAGACTCTCTGGATCGTCCACTGCAAAGGACACAGCTCTTGGCATTCAGCACCTacc                                   >  1:553808/1‑98 (MQ=255)
                                                                  ggCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACa                                  <  2:883436/100‑1 (MQ=255)
                                                                   gCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACAt                                 >  2:2981475/1‑100 (MQ=255)
                                                                   gCGTTACGCCTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACAt                                 >  2:3409281/1‑100 (MQ=255)
                                                                    cGTTACACCTTTACTTCACGTACAGCCACCGTTAACGCGCTAGAATTTCTGGATCGTGTACTGCACAAAACCCAGCACTGGCAATTCAGGACATAACa                                  >  1:1245917/1‑98 (MQ=255)
                                                                        aCGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGAt                            >  2:3463748/1‑100 (MQ=255)
                                                                         cGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATc                           <  2:64015/100‑1 (MQ=255)
                                                                          gCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCg                          <  2:1397021/100‑1 (MQ=255)
                                                                           ccTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGt                         >  1:2677954/1‑100 (MQ=255)
                                                                           ccTTTACGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACACAACCCAGCGCTGGCCATTCAGGACTTAACATCTGATCg                          <  1:2636783/99‑1 (MQ=255)
                                                                                aCGTTGCGTACTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATa                    <  1:600684/100‑1 (MQ=255)
                                                                                     gCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGtt               >  1:2975446/1‑100 (MQ=255)
                                                                                     gCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGtt               <  2:1866928/100‑1 (MQ=255)
                                                                                      cGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTc              <  2:1654933/100‑1 (MQ=255)
                                                                                      cGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTc              >  2:1322834/1‑100 (MQ=255)
                                                                                        tACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCaa            >  1:1699211/1‑100 (MQ=255)
                                                                                        tACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCaa            >  2:1569688/1‑100 (MQ=255)
                                                                                        tACTGACGCCGTTAACGCGATAGAATTTCTGAATCGTGTACTGCACAGAACCCAGCGCTCGCCATTCAGGACGTACCCTATGATCGTAAATACGGTTCaa            >  1:1548259/1‑100 (MQ=255)
                                                                                         aCTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAAt           >  1:437442/1‑100 (MQ=255)
                                                                                         aCTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAAt           <  1:3519735/100‑1 (MQ=255)
                                                                                          cTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCATCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATg          >  2:3363016/1‑100 (MQ=255)
                                                                                          cTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATg          <  2:3686902/100‑1 (MQ=255)
                                                                                          cTGCCGCCGCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATg          >  2:2363971/1‑100 (MQ=255)
                                                                                            gccgacGTTAACGCGATAGAATTTCTGGATCGTGTACCGCACAGAACCCAGCGCTAGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATg          >  1:2093866/1‑98 (MQ=255)
                                                                                             ccgccgTTAACGCGATAGAGTTTCTGGATCGTGTACTCCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCaacgta        >  1:2283977/1‑95 (MQ=255)
                                                                                             ccgccgCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAAc       >  2:1980920/1‑100 (MQ=255)
                                                                                             ccgccgCTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAAc       <  2:2700911/100‑1 (MQ=255)
                                                                                              cgccgTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACg      <  2:2585565/100‑1 (MQ=255)
                                                                                              cgccgTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAAACCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACg      >  1:4028585/1‑100 (MQ=255)
                                                                                                  gTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTAt  <  2:1326742/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
AGCGCGTTATCTTCGAATTTCTCACCCGTTTCCGTTTCTTCATTACCCGTCGGCATGATGATGTCTGGCGTTACGCCTTTACGTTGCGTACTGCCGCCGTTAACGCGATAGAATTTCTGGATCGTGTACTGCACAGAACCCAGCGCTGGCCATTCAGGACGTAACATCTGATCGTAAATACGGTTCAATGAACGGTAT  >  NC_000913/1913174‑1913371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: