Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,925,214 T→C 43.2% I36T (ATC→ACC)  holE → DNA polymerase III, theta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,925,2140TC43.2% 68.1 / 179.1 153I36T (ATC→ACC) holEDNA polymerase III, theta subunit
Reads supporting (aligned to +/- strand):  ref base T (38/48);  new base C (29/37);  total (68/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 157 total aligned reads displayed.

AGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTT  >  NC_000913/1925115‑1925313
                                                                                                   |                                                                                                   
agaaTCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGAt                                                                                                     <  1:3477118/100‑1 (MQ=255)
 gaaTCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATc                                                                                                    >  1:3900207/1‑100 (MQ=255)
 gaaTCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATc                                                                                                    <  2:1461714/100‑1 (MQ=255)
   aTCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGc                                                                                                  <  1:3503905/100‑1 (MQ=255)
   aTCTAGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGc                                                                                                  <  1:2449791/100‑1 (MQ=255)
    tCTGGCGAAACTGGATCGAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGTCTGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGc                                                                                                  >  1:3841420/1‑99 (MQ=255)
     cTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTg                                                                                                >  1:828269/1‑100 (MQ=255)
      tGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGa                                                                                               >  2:725447/1‑100 (MQ=255)
          tAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGc                                                                                            <  2:3988668/99‑1 (MQ=255)
          tAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCg                                                                                           <  1:2938467/100‑1 (MQ=255)
          tAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCg                                                                                           >  1:3903433/1‑100 (MQ=255)
           aaaCTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCgg                                                                                          >  2:109456/1‑100 (MQ=255)
             aCTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGtt                                                                                        <  2:566899/100‑1 (MQ=255)
             aCTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGtt                                                                                        >  2:3820859/1‑100 (MQ=255)
             aCTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGtt                                                                                        >  1:1444809/1‑100 (MQ=255)
              cTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTg                                                                                       <  1:788719/100‑1 (MQ=255)
                ggATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTTCAGCGGtcacc                                                                                     <  2:2830751/100‑5 (MQ=255)
                 gATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAAc                                                                                    >  1:3738466/1‑100 (MQ=255)
                  aTCAAACAGAAATGGATAAAGTGAATGTCTATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAAGATGCCGGTGACCGCTGAAGCGGTTGaa                                                                                     <  2:2442100/98‑1 (MQ=255)
                  aTCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACg                                                                                   <  1:284740/100‑1 (MQ=255)
                   tCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGGATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAAAGt                                                                                  <  1:3747070/100‑1 (MQ=255)
                   tCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGt                                                                                  >  1:2131942/1‑100 (MQ=255)
                    cAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTg                                                                                 >  1:684659/1‑100 (MQ=255)
                     aaaCAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGa                                                                                >  1:461773/1‑100 (MQ=255)
                     aaaCAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGAGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGa                                                                                <  1:3006894/100‑1 (MQ=255)
                     aaaCAGAAATAGATAAAGTGAATGGCGATTTGGCGGCGGCAGGGGTGGCAGTTAAAGAACGCTACAATGTGCGGGTGACCGCTGAAGCGGTTGAACGTGa                                                                                >  1:1232264/1‑100 (MQ=255)
                      aaCAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGaa                                                                               <  1:925288/100‑1 (MQ=255)
                       aCAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAAc                                                                              <  2:4228272/100‑1 (MQ=255)
                          gAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGc                                                                           >  1:290469/1‑100 (MQ=255)
                           cgatctaTAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGcc                                                                          <  1:449131/94‑1 (MQ=255)
                            aaTGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGAGGCATTTAAAGAAGGGTAAAATATGCCGGTGAGGGATGGAGCGGATGAATGTGAAGACCCt                                                                         >  1:3582272/1‑100 (MQ=255)
                             aTGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTg                                                                        <  2:4270324/100‑1 (MQ=255)
                                gATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAAc                                                                     <  2:2124231/100‑1 (MQ=255)
                                 aTAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGccagatcg                                                                    >  2:449133/1‑94 (MQ=255)
                                 aTAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACa                                                                    >  2:539436/1‑100 (MQ=255)
                                    aaGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACAttt                                                                 >  2:2188145/1‑100 (MQ=255)
                                    aaGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACAttt                                                                 >  2:2661482/1‑100 (MQ=255)
                                    aaGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCAGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACAttt                                                                 >  1:3003715/1‑100 (MQ=255)
                                     aGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATTGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTg                                                                <  2:3015622/100‑1 (MQ=255)
                                     aGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTg                                                                <  1:4044767/100‑1 (MQ=255)
                                     aGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTg                                                                >  2:4153572/1‑100 (MQ=255)
                                       tGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTgcg                                                              >  2:1254909/1‑100 (MQ=255)
                                       tGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTgcg                                                              <  2:2053822/100‑1 (MQ=255)
                                       tGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTgcg                                                              <  2:3746568/100‑1 (MQ=255)
                                        gAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTgcgc                                                             <  1:3047387/100‑1 (MQ=255)
                                         aaTGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCa                                                            <  1:1969392/100‑1 (MQ=255)
                                             tCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTg                                                        <  2:2101346/100‑1 (MQ=255)
                                             tCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTg                                                        <  2:1279137/100‑1 (MQ=255)
                                             tCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTg                                                        <  2:4026458/100‑1 (MQ=255)
                                             tCGATTTGGCGGCGGCCGGGGCGGCATGTAAAGAACGCCACAGTATGCCGGTGACGGCTGCAGCAGTTGCACGTGAACAGCCCGTACCTGTgcgc                                                             >  1:2067155/1‑95 (MQ=255)
                                              cGATTTGGCGGCGGCCGGTGTGGCATTTAAAGAACGGTACAAGATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTg                                                        <  1:1503397/99‑1 (MQ=255)
                                              cGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTgg                                                       >  2:2279269/1‑100 (MQ=255)
                                               gATTTGGCGGCGGCCGGGGTGGCATTTAAAGAATGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGt                                                      >  1:2556124/1‑100 (MQ=255)
                                                   tGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTcg                                                  >  1:2451708/1‑100 (MQ=255)
                                                        gcggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGc                                             <  1:1759091/100‑1 (MQ=255)
                                                        gcggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGc                                             >  2:3320093/1‑100 (MQ=255)
                                                        gcgccCGGCGTGGCATTTAAAGAACCCTACAATATGCCGGTAACCGCTAAAGCGGTTCAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGc                                             <  2:2481042/100‑1 (MQ=255)
                                                         cggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGCACGTGAACACCCTGAACATTTGCGCAGCTGGTTTCGCGAGCg                                            >  2:3701623/1‑100 (MQ=255)
                                                         cggcCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCg                                            <  2:4178/100‑1 (MQ=255)
                                                            ccGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCt                                         >  2:1189699/1‑100 (MQ=255)
                                                            ccGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCt                                         <  2:2428951/100‑1 (MQ=255)
                                                              ggggTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTa                                       <  2:2870487/100‑1 (MQ=255)
                                                               gggTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTAt                                      <  1:3336029/100‑1 (MQ=255)
                                                                ggTGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTAtt                                     <  2:181339/100‑1 (MQ=255)
                                                                 gTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTg                                    <  1:2279263/100‑1 (MQ=255)
                                                                  tGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGc                                   <  2:25849/100‑1 (MQ=255)
                                                                  tGGCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGc                                   <  2:3157176/100‑1 (MQ=255)
                                                                   ggCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGcc                                  <  2:3161676/100‑1 (MQ=255)
                                                                   ggCATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGcc                                  >  2:10700/1‑100 (MQ=255)
                                                                    gCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGccc                                 <  1:2826199/100‑1 (MQ=255)
                                                                    gCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCAGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGccc                                 >  1:2336389/1‑100 (MQ=255)
                                                                     cATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCa                                >  2:740375/1‑100 (MQ=255)
                                                                     cATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCa                                >  2:2303327/1‑100 (MQ=255)
                                                                     cATTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCa                                >  2:3622752/1‑100 (MQ=255)
                                                                      aTTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCAc                               >  2:3122085/1‑100 (MQ=255)
                                                                      aTTTAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTAGCGAGCGGCTTATTGCCCAc                               >  1:2917623/1‑100 (MQ=255)
                                                                      aTTTAAAGAACGCTACAATAAGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGccc                                 <  1:2564582/98‑1 (MQ=255)
                                                                         tAAAGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGt                            >  1:1787759/1‑100 (MQ=255)
                                                                            aGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTg                         <  2:828046/100‑1 (MQ=255)
                                                                            aGAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTg                         <  2:11041/100‑1 (MQ=255)
                                                                             gAACGCTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTgg                        >  2:3264752/1‑100 (MQ=255)
                                                                                 gCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACTTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTc                    <  2:3003727/100‑1 (MQ=255)
                                                                                 gCTACAATATGCCGGGGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACAGTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTc                    >  1:1030475/1‑100 (MQ=255)
                                                                                  cTACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCg                   >  1:3623932/1‑100 (MQ=255)
                                                                                  cTAAAATATGCGGGTGACCGCTGTAGCGGTTGAGCGTGGACAGCCTGAACATTTGCGCCGCTTGGTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCg                   >  1:1261041/1‑100 (MQ=255)
                                                                                   tACAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCgg                  <  1:1404845/100‑1 (MQ=255)
                                                                                    aCAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGt                 <  1:2264850/100‑1 (MQ=255)
                                                                                     cAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTc                >  2:1320747/1‑100 (MQ=255)
                                                                                     cAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTc                <  2:3569533/100‑1 (MQ=255)
                                                                                     cAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTc                <  1:3093473/100‑1 (MQ=255)
                                                                                     cAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTc                <  2:2832196/100‑1 (MQ=255)
                                                                                     cAATATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCCTCGGTc                >  2:1832131/1‑100 (MQ=255)
                                                                                      aaTATGCCGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCa               <  2:1630265/100‑1 (MQ=255)
                                                                                            ccGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCATTCTg          >  2:163514/1‑99 (MQ=255)
                                                                                             cGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTc        <  2:2131947/100‑1 (MQ=255)
                                                                                             cGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTc        <  2:2904141/100‑1 (MQ=255)
                                                                                             cGGTGACCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTc        <  1:1463710/100‑1 (MQ=255)
                                                                                              ggTGATCGCTGAAGCGGTTGAGCGTGAACAGCCTGAACTTTTGCGCAGCTGGTTGCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTc        <  1:4247434/99‑1 (MQ=255)
                                                                                                 gATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGt    >  1:107771/1‑100 (MQ=255)
                                                                                                   tCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGtt   >  2:1703793/1‑99 (MQ=255)
                                                                                                   |                                                                                                   
AGAATCTGGCTAAACTGGATCAAACAGAAATGGATAAAGTGAATGTCGATTTGGCGGCGGCCGGGGTGGCATTTAAAGAACGCTACAATATGCCGGTGATCGCTGAAGCGGTTGAACGTGAACAGCCTGAACATTTGCGCAGCTGGTTTCGCGAGCGGCTTATTGCCCACCGTTTGGCTTCGGTCAATCTGTCACGTTT  >  NC_000913/1925115‑1925313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: