Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,946,955 G→A 49.8% A141V (GCC→GTC)  ruvC ← component of RuvABC resolvasome, endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,946,9550GA49.8% 9.1 / 208.1 152A141V (GCC→GTC) ruvCcomponent of RuvABC resolvasome, endonuclease
Reads supporting (aligned to +/- strand):  ref base G (41/35);  new base A (37/38);  total (79/73)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 159 total aligned reads displayed.

AACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGTTT  >  NC_000913/1946857‑1947051
                                                                                                  |                                                                                                
aaCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATcggc                                                                                                 <  2:1827098/100‑1 (MQ=255)
 aCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATcggcg                                                                                                <  2:2030872/100‑1 (MQ=255)
 aCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATcgacg                                                                                                <  1:1174391/100‑1 (MQ=255)
  cGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATcgacgg                                                                                               >  2:490325/1‑100 (MQ=255)
  cGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATcgacgg                                                                                               <  2:2742749/100‑1 (MQ=255)
      gttgCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATc                                                                                           <  2:1324753/97‑1 (MQ=255)
      gTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATc                                                                                           <  2:3683379/100‑1 (MQ=255)
        cGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCg                                                                                         >  1:1793882/1‑100 (MQ=255)
          cccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGcc                                                                                       >  1:1034554/1‑100 (MQ=255)
          cccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGcc                                                                                       <  2:1950572/100‑1 (MQ=255)
          cccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGcc                                                                                       <  1:1560336/100‑1 (MQ=255)
          cccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGcc                                                                                       >  1:317576/1‑100 (MQ=255)
          cccTATCGCCAGGATCAGCCGCGATTCGCTCATCTGCATCGCATTCTGCCTCACGTGGCACGGGCTGATAGCAATCGCCCTCGCATCGCCGGCAGCCGc                                                                                        >  1:2641363/1‑99 (MQ=255)
           ccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCt                                                                                      >  2:2447141/1‑100 (MQ=255)
              ctcGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCtgtg                                                                                   >  1:814844/1‑100 (MQ=255)
                cGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGa                                                                                 <  2:4154131/100‑1 (MQ=255)
                cGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGa                                                                                 >  1:303458/1‑100 (MQ=255)
                cGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGa                                                                                 <  1:3466439/100‑1 (MQ=255)
                cGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGa                                                                                 >  2:1276472/1‑100 (MQ=255)
                cGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGa                                                                                 <  2:2367615/100‑1 (MQ=255)
                 gCCAGGTTCAGCCGCGATTCGCTCATCTGAATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGGGGAt                                                                                >  1:1415860/1‑100 (MQ=255)
                  ccAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGGCTGTGGAtt                                                                               >  1:1364507/1‑100 (MQ=255)
                    aGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAg                                                                             <  2:872430/100‑1 (MQ=255)
                        tCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCggg                                                                         <  2:3001831/100‑1 (MQ=255)
                        tCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCggg                                                                         <  1:1666873/100‑1 (MQ=255)
                         cAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGc                                                                        >  2:4235859/1‑100 (MQ=255)
                           gCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAg                                                                      >  2:2697364/1‑100 (MQ=255)
                           gCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAg                                                                      >  1:2869646/1‑100 (MQ=255)
                            ccGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGt                                                                     <  2:1873529/100‑1 (MQ=255)
                               cgATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTc                                                                  >  2:1933945/1‑100 (MQ=255)
                                gATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCa                                                                 <  1:1241499/100‑1 (MQ=255)
                                  ttCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGc                                                               >  2:431144/1‑100 (MQ=255)
                                  ttCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGc                                                               <  2:945157/100‑1 (MQ=255)
                                   tCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCa                                                              <  2:3181439/100‑1 (MQ=255)
                                    cGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCaa                                                             >  1:1299267/1‑100 (MQ=255)
                                     gCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAg                                                            >  2:38950/1‑100 (MQ=255)
                                         aTCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGc                                                        <  2:2379875/100‑1 (MQ=255)
                                         aTCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTg                                                         <  1:2411888/99‑1 (MQ=255)
                                          tCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCg                                                       <  1:962082/100‑1 (MQ=255)
                                          tCTGCAGCGCATTCTGTCTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCAGCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTg                                                         <  1:836377/98‑1 (MQ=255)
                                           cTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCgg                                                      >  2:426781/1‑100 (MQ=255)
                                            tGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGa                                                     >  2:3549966/1‑100 (MQ=255)
                                            tGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGa                                                     <  1:2210481/100‑1 (MQ=255)
                                            tGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGa                                                     >  1:2862301/1‑100 (MQ=255)
                                             gCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGAc                                                    <  1:436687/100‑1 (MQ=255)
                                             gCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGAc                                                    >  2:1439547/1‑100 (MQ=255)
                                              cATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGAcc                                                   >  1:1027875/1‑100 (MQ=255)
                                               aTCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCa                                                  >  2:1100553/1‑100 (MQ=255)
                                               aTCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCa                                                  >  2:3657292/1‑100 (MQ=255)
                                                tCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCat                                                 <  2:3379065/100‑1 (MQ=255)
                                                tCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCat                                                 <  2:1249872/100‑1 (MQ=255)
                                                 cGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCata                                                <  2:3276705/100‑1 (MQ=255)
                                                   cATTCTGACTAACGTGGCAGTGGGTTATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCata                                                <  1:414941/98‑1 (MQ=255)
                                                      tCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGCCCATAtgctg                                           >  1:203681/1‑100 (MQ=255)
                                                       cTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATAtgctg                                           >  1:2900673/1‑99 (MQ=255)
                                                         gACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCAc                                        >  2:3374108/1‑100 (MQ=255)
                                                              aCGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGc                                   <  1:216784/100‑1 (MQ=255)
                                                              aCGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGc                                   >  2:4017187/1‑100 (MQ=255)
                                                                gTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCtt                                 >  1:3743474/1‑100 (MQ=255)
                                                                  ggCAGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCtttt                               >  1:1359947/1‑100 (MQ=255)
                                                                     aGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTc                            <  2:661370/100‑1 (MQ=255)
                                                                     aGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTc                            <  1:1721564/100‑1 (MQ=255)
                                                                     aGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTc                            <  2:340598/100‑1 (MQ=255)
                                                                     aGTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTc                            <  1:2200640/100‑1 (MQ=255)
                                                                      gTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCg                           <  1:611441/100‑1 (MQ=255)
                                                                      gTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCg                           <  1:363476/100‑1 (MQ=255)
                                                                      gTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCg                           >  2:77498/1‑100 (MQ=255)
                                                                      gTGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCg                           <  2:1684204/100‑1 (MQ=255)
                                                                       tGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCgg                          <  2:1529864/100‑1 (MQ=255)
                                                                       tGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCgg                          >  2:140813/1‑100 (MQ=255)
                                                                       tGGGTGATAGCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCgg                          <  2:3354864/100‑1 (MQ=255)
                                                                        gggTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGc                         >  1:325606/1‑100 (MQ=255)
                                                                          gTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCAc                       >  1:3371687/1‑100 (MQ=255)
                                                                          gTGATAGCAAGCGCCAGCGCATCGGCGGGATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCAc                       <  1:795342/100‑1 (MQ=255)
                                                                           tGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACt                      >  1:642090/1‑100 (MQ=255)
                                                                              tAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGcc                   <  2:2199437/100‑1 (MQ=255)
                                                                              tAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGca                   <  1:3743339/100‑2 (MQ=255)
                                                                              tAGCAATCGCAAGCACATCGGCGGAATACGACTCTGAATTAGCGGGCAGTTTCAACACGGTGCGGATCAAATGCTGGACATGGCTTTTTTCGTCACTGc                    >  1:3898704/1‑99 (MQ=255)
                                                                               aGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCttttttttttttttt                    >  1:2738141/1‑89 (MQ=255)
                                                                               aGCAACCGCCAGCGCATCGGCGGCATCCGCCTGGGGATTAGCGGGCAGTTCCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCa                  <  1:680750/100‑1 (MQ=255)
                                                                                gCAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCaa                 >  2:3981148/1‑100 (MQ=255)
                                                                                 cAATCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAt                >  2:1091404/1‑100 (MQ=255)
                                                                                  aaTCGCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAta               >  1:3509662/1‑100 (MQ=255)
                                                                                     cGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAtacct            <  1:3571026/100‑1 (MQ=255)
                                                                                      gCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAtaccta           >  2:1385948/1‑100 (MQ=255)
                                                                                      gCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAtaccta           >  2:2961384/1‑100 (MQ=255)
                                                                                      gCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAtaccta           <  2:342153/100‑1 (MQ=255)
                                                                                      gCCAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAtaccta           >  2:2555903/1‑100 (MQ=255)
                                                                                       ccAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAtacctac          >  2:3833110/1‑100 (MQ=255)
                                                                                        cAGCGCATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAAtacctacc         <  1:1100550/100‑1 (MQ=255)
                                                                                         aGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACAATATGCTGCACCTGGATTTTTTCGGCACTGCCAATACCTacca        >  1:2540103/1‑100 (MQ=255)
                                                                                          gcgcATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTaccac       >  1:139625/1‑100 (MQ=255)
                                                                                           cgcATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTTGCTTTTTTCGGCACTGCCAATACCTaccacc      <  2:2873019/100‑1 (MQ=255)
                                                                                           cgcATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTaccacc      <  2:317578/100‑1 (MQ=255)
                                                                                           cgcATCGACGGCATCCGCCTGTGGATTAGCGGGCAGCTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTaccacc      <  2:3001162/100‑1 (MQ=255)
                                                                                            gcATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCg     <  2:216627/100‑1 (MQ=255)
                                                                                             cATCGACGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGt    >  1:1117205/1‑100 (MQ=255)
                                                                                              aTCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGtt   >  2:583915/1‑100 (MQ=255)
                                                                                               tCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGttt  <  1:1671574/100‑1 (MQ=255)
                                                                                               tCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGttt  <  1:1498939/100‑1 (MQ=255)
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AACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCAGCGCATCGGCGGCATCCGCCTGTGGATTAGCGGGCAGTTTCAGCAAGGTGCGGACCATATGCTGCACCTGGCTTTTTTCGGCACTGCCAATACCTACCACCGTTT  >  NC_000913/1946857‑1947051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: