Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,036,066 A→C 40.9% T78P (ACG→CCG)  hchA → glyoxalase III and Hsp31 molecular chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,036,0660AC40.9% 70.8 / 189.6 149T78P (ACG→CCG) hchAglyoxalase III and Hsp31 molecular chaperone
Reads supporting (aligned to +/- strand):  ref base A (45/43);  new base C (24/37);  total (69/80)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01

Only 100 of 150 total aligned reads displayed.

CGCGGTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTGGGCTATGCCG  >  NC_000913/2035970‑2036164
                                                                                                |                                                                                                  
cgcgGTAAACATAAGATTCTGGTGATCGCCGCGGACGAACGGTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGt                                                                                                 >  1:1375175/1‑100 (MQ=255)
  cgGTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAACCTGTTCTCGACCGGTAACCATCCGATTGAAACg                                                                                                  >  1:3774958/1‑97 (MQ=255)
   ggTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTtg                                                                                               >  1:808803/1‑99 (MQ=255)
   ggTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTtgc                                                                                              <  1:2064229/100‑1 (MQ=255)
       aaCATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGcc                                                                                          >  1:3983764/1‑100 (MQ=255)
       aaCATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGcc                                                                                          <  2:3222725/100‑1 (MQ=255)
         cATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGt                                                                                        >  1:3960660/1‑100 (MQ=255)
          aTAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGtt                                                                                       <  1:1274134/100‑1 (MQ=255)
          aTAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGtt                                                                                       <  2:4195907/100‑1 (MQ=255)
           tAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTg                                                                                      <  2:1629669/100‑1 (MQ=255)
           tAAAATTCTGGTGATCGCCGCGGACGAACGTTATTAGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAAAGTTGCTGCTGTTg                                                                                      >  1:1264349/1‑100 (MQ=255)
           tAAAATCCTGGTGACCGCAGCGTACGCACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCTACCGGTAATCATCCGATTGAAACGTTGCTGCCGt                                                                                        <  1:1494390/98‑1 (MQ=255)
             aaaTTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTa                                                                                    <  2:1994256/100‑1 (MQ=255)
             aaaTTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTa                                                                                    <  2:4124643/100‑1 (MQ=255)
             aaaTTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTa                                                                                    <  1:2266210/100‑1 (MQ=255)
                ttCTGGTGATCGCCGTGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTatca                                                                                 <  2:4206348/100‑1 (MQ=255)
                ttCTGGTGATCGCCGCGGACGAACGTTATTTGCCTACCGATAACGGAAACCTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTatca                                                                                 >  1:1744452/1‑100 (MQ=255)
                ttCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTatca                                                                                 >  2:3245412/1‑100 (MQ=255)
                ttCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTatca                                                                                 >  1:2374310/1‑100 (MQ=255)
                ttCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACAGTTCTCGACCGGTCACCATCCGATTGAACCGTTGCTGTCGTTGTatc                                                                                  >  1:1845874/1‑99 (MQ=255)
                  cTGGTAATCGCCGCGGACGAACGTAATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGATAACCATCCGATTGAACCGTTGCTGCAATAGTatca                                                                                 >  1:2127219/1‑98 (MQ=255)
                   tGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCAtct                                                                              <  2:2352665/100‑1 (MQ=255)
                    ggTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTatcatc                                                                               >  1:174181/1‑98 (MQ=255)
                    ggTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCAtctc                                                                             >  1:484862/1‑100 (MQ=255)
                       gATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCAt                                                                          >  1:3159984/1‑100 (MQ=255)
                          cgccgcGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCAtgct                                                                       <  2:1330160/100‑1 (MQ=255)
                           gccgcGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCAtgctg                                                                      <  2:1895151/100‑1 (MQ=255)
                            ccgcGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCAtgctgc                                                                     >  2:464621/1‑100 (MQ=255)
                              gcgGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAg                                                                   <  1:3871240/100‑1 (MQ=255)
                               cgGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAgg                                                                  <  2:74323/100‑1 (MQ=255)
                               cgGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAgg                                                                  <  2:125142/100‑1 (MQ=255)
                                ggACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAg                                                                   <  1:2523527/98‑1 (MQ=255)
                                ggACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGt                                                                 <  2:2002099/100‑1 (MQ=255)
                                   cGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttc                                                              <  1:2962586/100‑1 (MQ=255)
                                   cGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttc                                                              >  2:1134579/1‑100 (MQ=255)
                                    gAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcg                                                             >  1:184379/1‑100 (MQ=255)
                                     aaCGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTACATGCTGCAGGTttcga                                                            >  2:1280583/1‑100 (MQ=255)
                                      aCGTTATTTGCCGACCGATAACGGAAAACTGTTCTTGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcg                                                             <  1:2875436/98‑1 (MQ=255)
                                      aCGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcgaa                                                           <  1:2622864/100‑1 (MQ=255)
                                        gTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcgaatt                                                         >  1:217092/1‑100 (MQ=255)
                                        gTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcgaatt                                                         <  2:1574786/100‑1 (MQ=255)
                                           aTTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcgaattcga                                                      <  2:3087999/100‑1 (MQ=255)
                                           aTTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcgaattcga                                                      <  1:594430/100‑1 (MQ=255)
                                             ttGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGATTTCGAAg                                                    <  2:2244444/100‑1 (MQ=255)
                                              tGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGt                                                   <  1:104641/100‑1 (MQ=255)
                                              tGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGt                                                   >  1:4038275/1‑100 (MQ=255)
                                              tGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGt                                                   >  2:691042/1‑100 (MQ=255)
                                                ccgaccgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTgg                                                 >  1:4209856/1‑100 (MQ=255)
                                                ccgaccgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTgg                                                 >  2:821103/1‑100 (MQ=255)
                                                 cgaccgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGc                                                <  2:1704823/100‑1 (MQ=255)
                                                 cgaccgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGc                                                >  1:1336365/1‑100 (MQ=255)
                                                 cgaccgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTgg                                                 >  1:462383/1‑99 (MQ=255)
                                                 cgaccgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGc                                                >  2:1918256/1‑100 (MQ=255)
                                                 cgaccgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGACGTTGTATCATCTCCATGCTGCAGATTTCGAATTCGAAGTGGc                                                >  1:653788/1‑100 (MQ=255)
                                                   accgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGa                                              <  2:1697021/100‑1 (MQ=255)
                                                    ccgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGAc                                             <  2:3983784/100‑1 (MQ=255)
                                                     cgaTAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATATACATACTGAAGGTTTCGAATTCGAAGTGGCGatc                                            >  1:2687036/1‑98 (MQ=255)
                                                        tAACTGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGAcc                                            <  1:3864664/97‑1 (MQ=255)
                                                          aCGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTc                                       >  2:1785812/1‑100 (MQ=255)
                                                          aCGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTc                                       <  2:1820451/100‑1 (MQ=255)
                                                           cGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTcc                                      >  2:3173856/1‑100 (MQ=255)
                                                           cGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTcc                                      >  2:635040/1‑100 (MQ=255)
                                                            ggAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCg                                     >  1:1699276/1‑100 (MQ=255)
                                                             gAAAACTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCgg                                    >  2:2821762/1‑100 (MQ=255)
                                                             gAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCgg                                    <  1:2919678/100‑1 (MQ=255)
                                                             gAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCgg                                    <  1:3632637/100‑1 (MQ=255)
                                                              aaaaCTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGt                                   <  1:3713479/100‑1 (MQ=255)
                                                               aaaCTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTc                                  >  2:2838525/1‑100 (MQ=255)
                                                                 aCTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCACCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtg                                <  2:625197/100‑1 (MQ=255)
                                                                  cTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtga                               <  2:3279496/100‑1 (MQ=255)
                                                                  cTGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtga                               >  2:2962654/1‑100 (MQ=255)
                                                                  cTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtga                               >  2:1431726/1‑100 (MQ=255)
                                                                   tGTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgat                              >  1:3413682/1‑100 (MQ=255)
                                                                   tGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgat                              <  2:3740582/100‑1 (MQ=255)
                                                                    gTTCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgatg                             <  1:986496/100‑1 (MQ=255)
                                                                    gTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgatg                             >  1:795666/1‑100 (MQ=255)
                                                                     ttCTCGACCGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCtgatga                            <  2:4117855/100‑1 (MQ=255)
                                                                      tctcGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGAc                           <  1:4082789/100‑1 (MQ=255)
                                                                      tctcGAACGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATGATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGAc                           <  1:1334286/100‑1 (MQ=255)
                                                                          gACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAg                       >  1:4129679/1‑100 (MQ=255)
                                                                          gACCGGTAACCAGCCGATTTAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGAc                           <  1:1097522/96‑1 (MQ=255)
                                                                            ccGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGtt                     >  1:1763224/1‑100 (MQ=255)
                                                                            ccGGTAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGtt                     <  1:198913/100‑1 (MQ=255)
                                                                              ggTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATTACCAAGtt                     >  1:989014/1‑98 (MQ=255)
                                                                              ggTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTg                   >  1:1948716/1‑100 (MQ=255)
                                                                              ggTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTg                   >  2:398746/1‑100 (MQ=255)
                                                                                tAACCATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTAGCGACCATTTCCGGTCTGATGACCAAGTTTGaa                 <  1:2821751/100‑1 (MQ=255)
                                                                                 aaCCATCCGATTGAACCGTTGCGGCCGTTTTATGACACCCATCCCGGAGGTTTCGAATTCGAAGGGGCGACCATTTCCGGTCTGATGACCAAGttt                    <  1:1770144/96‑1 (MQ=255)
                                                                                 aaCCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAAt                <  2:2270524/100‑1 (MQ=255)
                                                                                  acgaTCCGATGGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCCGGTGTCGAACTCGAAGGGGCGACCATTTCCGGTCTGATGACCAAGTTTGa                  <  1:1917448/94‑1 (MQ=255)
                                                                                  aCCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATa               >  2:848821/1‑100 (MQ=255)
                                                                                  aCCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATa               <  2:3667064/100‑1 (MQ=255)
                                                                                   ccATCCGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATAc              <  2:3389970/100‑1 (MQ=255)
                                                                                     aTCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTg            >  2:3809418/1‑100 (MQ=255)
                                                                                     aTCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTg            >  2:2343240/1‑100 (MQ=255)
                                                                                        cGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTGGGc         <  1:2759402/100‑1 (MQ=255)
                                                                                        cGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTGGGc         <  1:315771/100‑1 (MQ=255)
                                                                                        cGATTGAACCGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTGGGc         <  2:1082355/100‑1 (MQ=255)
                                                                                          aTTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTGGGCTa       >  1:1633648/1‑100 (MQ=255)
                                                                                            tGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTGGGCTATg     >  2:52451/1‑100 (MQ=255)
                                                                                                |                                                                                                  
CGCGGTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCGAAGTGGCGACCATTTCCGGTCTGATGACCAAGTTTGAATACTGGGCTATGCCG  >  NC_000913/2035970‑2036164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: