Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 350,119 T→G 49.9% G36G (GGT→GGG)  prpC → 2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913350,1190TG49.9% 23.3 / 169.0 121G36G (GGT→GGG) prpC2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base T (29/31);  new base G (28/32);  total (57/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

ACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAA  >  NC_000913/350021‑350217
                                                                                                  |                                                                                                  
acaACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATGCGGCGCTCTGCACCGTGGGTa                                                                                                   <  1:3893988/100‑1 (MQ=255)
acaACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTa                                                                                                   <  1:85817/100‑1 (MQ=255)
 caACGATCCTGCAAAACAGTACCCATTTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTaa                                                                                                  >  2:3177705/1‑100 (MQ=255)
 caACGATCCTGCAAAACAGTACCCATGTCATTAAACCAAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCACTGCACCGTGGGTaa                                                                                                  >  1:2288055/1‑100 (MQ=255)
  aaCGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCATGGGTaaa                                                                                                 <  1:1144784/100‑1 (MQ=255)
     gATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGt                                                                                              <  1:2431380/100‑1 (MQ=255)
     gATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGt                                                                                              <  1:3536465/100‑1 (MQ=255)
       tCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTgg                                                                                            <  1:2356463/100‑1 (MQ=255)
        ccTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGc                                                                                           <  2:2125950/100‑1 (MQ=255)
        ccTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGc                                                                                           <  1:3873557/100‑1 (MQ=255)
        ccTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGc                                                                                           <  1:3064094/100‑1 (MQ=255)
        ccTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGc                                                                                           >  1:1911817/1‑100 (MQ=255)
         cTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCa                                                                                          <  1:1584418/100‑1 (MQ=255)
         cTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCa                                                                                          <  1:1964408/100‑1 (MQ=255)
         cTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCa                                                                                          <  2:3750890/100‑1 (MQ=255)
         cTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCCCTGCACCGTGGGTAAAAGTGGCa                                                                                          >  1:3303068/1‑100 (MQ=255)
           gCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAAt                                                                                        >  1:1329603/1‑100 (MQ=255)
           gCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAAt                                                                                        >  1:1865507/1‑100 (MQ=255)
                  aGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGc                                                                                 <  1:3082226/100‑1 (MQ=255)
                  aGTACCCATGTCATTAAACCGAAAAAATCTGTGACACTTTCTGGCGTTCCTGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGc                                                                                 >  2:1922497/1‑100 (MQ=255)
                  aGTACCCATGTCATTAAAACGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGc                                                                                 <  1:2449396/100‑1 (MQ=255)
                     aCCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAGTGAccgg                                                                                  >  1:2653334/1‑94 (MQ=255)
                      cccATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAGAAGTGGCAATGACCTGCAATa                                                                             >  1:3541495/1‑100 (MQ=255)
                       ccATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTAc                                                                            <  1:1229450/100‑1 (MQ=255)
                        cATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTAcc                                                                           <  1:2880763/100‑1 (MQ=255)
                         aTGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACcg                                                                          <  2:3311608/100‑1 (MQ=255)
                          tGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACcgc                                                                         >  1:44040/1‑100 (MQ=255)
                          tGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACcgc                                                                         <  1:3416500/100‑1 (MQ=255)
                            tCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCgg                                                                       <  1:3863658/100‑1 (MQ=255)
                            tCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCgg                                                                       <  2:3721738/100‑1 (MQ=255)
                            tCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCgg                                                                       <  2:3673918/100‑1 (MQ=255)
                              aTTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCt                                                                     <  2:3832313/100‑1 (MQ=255)
                              aTTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCt                                                                     <  2:2466253/100‑1 (MQ=255)
                                  aaCCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGa                                                                 >  1:16701/1‑100 (MQ=255)
                                   aCCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGat                                                                <  2:2768565/100‑1 (MQ=255)
                                    ccGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGata                                                               >  1:4190130/1‑100 (MQ=255)
                                    ccGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATCACCGCGGCTACGaaa                                                               >  2:3987496/1‑98 (MQ=255)
                                     cGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGatat                                                              >  1:3641692/1‑100 (MQ=255)
                                      gAAAAAATCTATGGCACTTTCTGGCGTTCCGGCGAAAAATCCGGAGCGCTGCACACTGGGGCACAGTGGCAATCAGCTGCGATACCGCGCCTACGaca                                                               >  1:3593143/1‑96 (MQ=255)
                                          aaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGGTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCGTTACCGCGGCTACGATATTCTTg                                                         <  1:3007303/100‑1 (MQ=255)
                                          aaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTg                                                         <  2:4000453/100‑1 (MQ=255)
                                           aaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGAGATTCTTGa                                                        >  1:2668219/1‑100 (MQ=255)
                                               tgtgGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTg                                                    <  2:116900/100‑1 (MQ=255)
                                                gtgGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCCTTACCGCGGCTACGATATTCTTGATc                                                      >  2:4270236/1‑97 (MQ=255)
                                                 tgGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGc                                                  <  2:3438625/100‑1 (MQ=255)
                                                 tgGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGc                                                  <  2:1132723/100‑1 (MQ=255)
                                                  ggCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGaaccg                                                    >  2:1583424/1‑93 (MQ=255)
                                                   gCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGa                                                >  1:1058998/1‑100 (MQ=255)
                                                     aCTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGaaa                                              <  2:3261084/100‑1 (MQ=255)
                                                      cTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCAGCGGGGGTAAAAGTGGCAATGACGTGCATTACCGCGGCTACGATATTCTTGATCTGGc                                                  >  1:1141012/1‑95 (MQ=255)
                                                      cTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAAc                                             >  1:474832/1‑100 (MQ=255)
                                                          cTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTg                                         >  1:257829/1‑100 (MQ=255)
                                                           tGGCGTTCCGGCGGGCAATACGGCGCTCTGCACGGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGc                                        >  1:2822447/1‑100 (MQ=255)
                                                           tGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGc                                        <  2:3963301/100‑1 (MQ=255)
                                                           tGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGc                                        <  2:1334964/100‑1 (MQ=255)
                                                            ggCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCg                                       <  2:2755270/100‑1 (MQ=255)
                                                            ggCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCg                                       <  2:546921/100‑1 (MQ=255)
                                                            ggCGTTCCGGCGGGCAATACGGCGCGCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTg                                         <  1:4037238/98‑1 (MQ=255)
                                                            ggCGTTCCGACGGGCAATACGGCGCTCTGCACCGTGGGGAGAAGTGGCAATGACATACATTACCGCGGCTCCGATATTCTTGAAATAGCGAAAAAtaacg                                       >  1:2309870/1‑96 (MQ=255)
                                                             gCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCg                                       <  2:1404725/99‑1 (MQ=255)
                                                              cGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGaa                                     >  1:4193101/1‑100 (MQ=255)
                                                                 tCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGCTCTGGCGAAACATTGCGAAt                                    >  1:2875042/1‑98 (MQ=255)
                                                                 tCCGGCGGGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAAttt                                  <  2:2252624/100‑1 (MQ=255)
                                                                    ggcggGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGCTCTGGCGAAACATTGCGAATTTg                                 >  1:1163532/1‑98 (MQ=255)
                                                                    ggcggGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaa                               <  2:4051341/100‑1 (MQ=255)
                                                                    ggcggGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaa                               <  2:3886796/100‑1 (MQ=255)
                                                                    ggcggGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaa                               >  1:1087207/1‑100 (MQ=255)
                                                                     gcggGCAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaag                              <  2:1719925/100‑1 (MQ=255)
                                                                       gggCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaagaa                            >  1:246480/1‑100 (MQ=255)
                                                                         gCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTACGAATTTGAAGAAGt                          >  1:3557416/1‑100 (MQ=255)
                                                                          cAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCCTTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTg                         >  1:3268055/1‑100 (MQ=255)
                                                                          cAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGaagaag                           >  1:1997512/1‑98 (MQ=255)
                                                                          cAATACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTg                         <  2:3858250/100‑1 (MQ=255)
                                                                           aaTACGGCGCACTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTTATGTGGCGAAACATTGCGAATTTGAAGAAGTgg                        <  1:2809350/100‑1 (MQ=255)
                                                                            aTACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgc                       <  2:3176561/100‑1 (MQ=255)
                                                                            aTACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgc                       <  2:474834/100‑1 (MQ=255)
                                                                            aTACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgc                       >  1:248581/1‑100 (MQ=255)
                                                                            aTACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCCGGCGAAACATTGCGCATTTGaagaag                           >  2:3805641/1‑96 (MQ=255)
                                                                             tACGGCGCTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcg                      <  2:3095428/100‑1 (MQ=255)
                                                                               cGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                     >  1:2983176/1‑99 (MQ=255)
                                                                               cGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCa                    <  2:1804717/100‑1 (MQ=255)
                                                                               cGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCa                    <  2:2687137/100‑1 (MQ=255)
                                                                                ggCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGCATTTGAAGAAGTGgc                       >  1:233226/1‑96 (MQ=255)
                                                                                 gcgcTCTGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATc                  >  1:919032/1‑100 (MQ=255)
                                                                                    ctctGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATctgc               >  1:2203485/1‑100 (MQ=255)
                                                                                    ctctGCACCGTGGGGAAAAGTGGCCATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGCACTTGAAGAAGTGGCGCATctgc               >  1:248535/1‑100 (MQ=255)
                                                                                    ctctGCACCGTGGG‑GAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATctgct              <  2:3226686/100‑1 (MQ=255)
                                                                                      ctGCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATGTGAAGAAGTGGCGCATctggtg             >  1:2421118/1‑100 (MQ=255)
                                                                                        gCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGCATTTGAAGACGTGGCGCATCTGCTGAt           >  1:2511318/1‑100 (MQ=255)
                                                                                        gCACCGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGAt           <  2:1598873/100‑1 (MQ=255)
                                                                                           ccGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCa        <  2:1998861/100‑1 (MQ=255)
                                                                                           ccGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATCGCGAAGTTGAAGAAGTGGCGCATCTGCTGATCCa        >  2:3203955/1‑100 (MQ=255)
                                                                                           ccGTGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATAATCTCGATCTGGCGAAACATTGCGAGTTTGTAGAGGTGGCGCAGCTGCTGATCCa        >  1:1417381/1‑100 (MQ=255)
                                                                                             gTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACg      <  2:696987/100‑1 (MQ=255)
                                                                                             gTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATGGCGAATTTGAAGAAGTGGCGCATCTGCTGATCAAc       >  1:3930888/1‑99 (MQ=255)
                                                                                              tGGGGAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACgg     <  2:1403226/100‑1 (MQ=255)
                                                                                               gggTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGc    >  1:1827190/1‑100 (MQ=255)
                                                                                                ggTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCa   >  1:3484350/1‑100 (MQ=255)
                                                                                                 gTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCaa  >  1:2531399/1‑100 (MQ=255)
                                                                                                 gTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATAGCGAAGTTGAAGAAGTGGCGCATCTGATGATCCACGGCa   >  1:550747/1‑99 (MQ=255)
                                                                                                  |                                                                                                  
ACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAA  >  NC_000913/350021‑350217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑