Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,181,824 A→G 42.0% A243A (GCA→GCG)  yegV → putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,181,8240AG42.0% 73.9 / 209.4 173A243A (GCA→GCG) yegVputative kinase
Reads supporting (aligned to +/- strand):  ref base A (54/46);  new base G (38/34);  total (92/81)
Fisher's exact test for biased strand distribution p-value = 8.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.03e-01

Only 100 of 180 total aligned reads displayed.

CGAACGTTTTGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTT  >  NC_000913/2181725‑2181923
                                                                                                   |                                                                                                   
cgAACGTTTTGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGTAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCa                                                                                                     <  2:3447022/100‑1 (MQ=255)
cgAACGTTTTGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCa                                                                                                     >  1:714455/1‑100 (MQ=255)
cgAACGTTTTGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCa                                                                                                     >  1:371371/1‑100 (MQ=255)
 gAACGTTTTGCTTTATCCGCAGAGATAACAACACGTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCAt                                                                                                    <  1:1885609/100‑1 (MQ=255)
  aaCGTTTTGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATg                                                                                                   <  1:2060212/100‑1 (MQ=255)
  aaCGTTTTGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATg                                                                                                   <  1:3978440/100‑1 (MQ=255)
   aCGTTTTGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATgg                                                                                                  <  2:3228266/100‑1 (MQ=255)
      ttttGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTTCCGCGCCGTTTATCGTTCGTCTCGATAAAGAAGGCGCGTGGTa                                                                                                >  1:3565815/1‑99 (MQ=255)
      ttttGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTAt                                                                                               >  1:1613779/1‑100 (MQ=255)
        ttGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTAttt                                                                                             <  1:632249/100‑1 (MQ=255)
         tGCTTTATCCGCAGATATAACAACACTTGGCAAGGAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTc                                                                                            <  1:3675623/100‑1 (MQ=255)
         tGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTc                                                                                            <  1:1257642/100‑1 (MQ=255)
           cTTTATCCGGAGAGATAACAACACTTTGCAAGCAATGGCAGGAGATATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAg                                                                                          <  1:4114174/100‑1 (MQ=255)
           cTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAg                                                                                          >  1:740686/1‑100 (MQ=255)
           cTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAg                                                                                          <  1:3303848/100‑1 (MQ=255)
           cTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAg                                                                                          >  1:4019629/1‑100 (MQ=255)
            tttATCCTCAGAGATAACAACACTTGGCGAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGCTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAg                                                                                          <  1:2559959/99‑1 (MQ=255)
               aTCCGCAGAGATAACAACACTTAGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAac                                                                                      >  1:2481178/1‑100 (MQ=255)
                tCCGCAGAGATAACAACACTTCGCAAGCAATGGCAAGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGAGAAAGAAGGAGCATGGTAGTTCAGc                                                                                         >  1:1787256/1‑96 (MQ=255)
                 ccGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAacga                                                                                    >  2:425136/1‑100 (MQ=255)
                  cGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAacgac                                                                                   >  2:4002279/1‑100 (MQ=255)
                  cGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAacgac                                                                                   >  1:3515991/1‑100 (MQ=255)
                   gCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAacgacg                                                                                  >  2:3550194/1‑100 (MQ=255)
                   gCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAacgacg                                                                                  >  2:1410303/1‑100 (MQ=255)
                   gCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAacgacg                                                                                  >  2:2794821/1‑100 (MQ=255)
                    cAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGc                                                                                 >  1:4257998/1‑100 (MQ=255)
                       agaTAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTc                                                                              <  1:2902729/100‑1 (MQ=255)
                          tAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTgg                                                                           >  2:1221108/1‑100 (MQ=255)
                           aacaacACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGc                                                                          <  2:1990109/100‑1 (MQ=255)
                             caacaCTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTg                                                                        <  2:268147/100‑1 (MQ=255)
                             caacaCTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTg                                                                        >  1:1051207/1‑100 (MQ=255)
                              aacaCTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGc                                                                       <  2:1065881/100‑1 (MQ=255)
                               acacTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTg                                                                        <  1:1518166/98‑1 (MQ=255)
                                cacTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCAt                                                                     >  2:3572227/1‑100 (MQ=255)
                                cacTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCAt                                                                     >  2:1467279/1‑100 (MQ=255)
                                       caagcaaTGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCa                                                              <  2:1903698/100‑1 (MQ=255)
                                         agcaaTGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCAtt                                                            >  2:3557774/1‑100 (MQ=255)
                                          gcaaTGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCAttt                                                           >  2:621663/1‑100 (MQ=255)
                                            aaTGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTcc                                                         <  2:663369/100‑1 (MQ=255)
                                            aaTGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCACATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTcc                                                         <  1:2830693/100‑1 (MQ=255)
                                             aTGGCAGGAGAGATTGGCCGCGCCGGTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGCCGCTTCTGGCTGCATTCCGGCATTTcc                                                         <  1:1668151/99‑1 (MQ=255)
                                               ggCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAAc                                                      <  1:3431678/100‑1 (MQ=255)
                                               ggCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAAc                                                      <  2:610223/100‑1 (MQ=255)
                                                 cAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGc                                                    >  1:432981/1‑100 (MQ=255)
                                                  aGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCa                                                   >  2:2835080/1‑100 (MQ=255)
                                                   ggAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCaa                                                  >  2:3260593/1‑100 (MQ=255)
                                                    gagaAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAg                                                 <  2:1112697/100‑1 (MQ=255)
                                                     agaAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTACATTCCGGCATTTCCAACGCAAGt                                                <  2:2007070/100‑1 (MQ=255)
                                                     agaAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAg                                                 <  1:3376765/99‑1 (MQ=255)
                                                       aaaTTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTg                                              <  2:2369646/100‑1 (MQ=255)
                                                        aaTTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGt                                             >  2:1677406/1‑100 (MQ=255)
                                                         aTTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTc                                            <  2:365413/100‑1 (MQ=255)
                                                         aTTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTc                                            >  2:312819/1‑100 (MQ=255)
                                                         aTTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTc                                            <  1:781771/100‑1 (MQ=255)
                                                         aTTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTc                                            >  1:1070663/1‑100 (MQ=255)
                                                            tGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGac                                         <  1:2455958/100‑1 (MQ=255)
                                                              ccGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGacac                                       >  2:3074394/1‑100 (MQ=255)
                                                              ccGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGacac                                       >  2:1561409/1‑100 (MQ=255)
                                                               cgcgCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACAcc                                      <  1:2921508/100‑1 (MQ=255)
                                                                       tGATCGTTCGTCTCGATAAGGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGgggc                              <  1:2299254/100‑1 (MQ=255)
                                                                       tGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGgggc                              >  1:2749550/1‑100 (MQ=255)
                                                                        gATCGTTCGTCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGgggcg                             >  1:3260522/1‑100 (MQ=255)
                                                                         aTCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGgggcgg                            >  2:1707087/1‑100 (MQ=255)
                                                                          tcgttcgtCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGgggcggg                           <  2:2975590/100‑1 (MQ=255)
                                                                           cgttcgtCTCGATAAAGAAGGCGCTTGTTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGgggcgggc                          <  1:998336/100‑1 (MQ=255)
                                                                           cgttcgtCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGgggcgggc                          >  1:2413825/1‑100 (MQ=255)
                                                                           cgttcgtCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACTCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGCCACCATTGgggcgggc                          <  1:3843133/100‑1 (MQ=255)
                                                                              tcgtCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGAc                       <  2:92625/100‑1 (MQ=255)
                                                                               cgtCTCGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGgggcagatcgga                      >  2:802332/1‑92 (MQ=255)
                                                                               cctctcGATAAAGAAGGCGCATGGTATGTTAGCAACGGCGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTgg                                 <  1:4009874/87‑1 (MQ=255)
                                                                                gtCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAg                     >  2:2388434/1‑100 (MQ=255)
                                                                                gtCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAg                     <  1:1324895/100‑1 (MQ=255)
                                                                                 tctcGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGt                    >  2:5592/1‑100 (MQ=255)
                                                                                 tctcGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGt                    >  1:2474988/1‑100 (MQ=255)
                                                                                 tctcGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGt                    <  2:1776112/100‑1 (MQ=255)
                                                                                 tctcGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGt                    <  2:1182297/100‑1 (MQ=255)
                                                                                   tcGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCa                  <  2:2700429/100‑1 (MQ=255)
                                                                                   tcGATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCa                  >  1:2031950/1‑100 (MQ=255)
                                                                                   tcGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCa                  >  1:1516422/1‑100 (MQ=255)
                                                                                    cGATAAAGACGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTc                   >  1:1734306/1‑98 (MQ=255)
                                                                                    cGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCAt                 >  2:2956509/1‑100 (MQ=255)
                                                                                    cGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCAt                 >  2:3107533/1‑100 (MQ=255)
                                                                                     gATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATg                >  2:2451832/1‑100 (MQ=255)
                                                                                     gATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATg                <  2:3192991/100‑1 (MQ=255)
                                                                                     gATAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATg                >  1:569652/1‑100 (MQ=255)
                                                                                     gATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCAt                 >  1:1588473/1‑99 (MQ=255)
                                                                                       tAAAGCAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTGATGc               <  1:3831487/99‑1 (MQ=255)
                                                                                       tAAAGAAGGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGcc              <  2:4085752/100‑1 (MQ=255)
                                                                                       tAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGcc              <  2:4045539/100‑1 (MQ=255)
                                                                                        aaaGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCg             >  2:3275199/1‑100 (MQ=255)
                                                                                         aagaagGCGCGTGGTATGTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTACAACGCAAGTTCTCGACCCCATTGGGGCGGGCGACAGTCATGCCgg            >  1:308843/1‑100 (MQ=255)
                                                                                         aagaagGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCgg            <  1:1766484/100‑1 (MQ=255)
                                                                                            aagGCGCGTGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTgg         <  2:2031954/100‑1 (MQ=255)
                                                                                             agGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGc        >  2:3878824/1‑100 (MQ=255)
                                                                                              ggCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCg       <  1:157006/100‑1 (MQ=255)
                                                                                              ggCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCg       >  2:2197623/1‑100 (MQ=255)
                                                                                              ggCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCAGTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCTGTGGCg       <  1:300253/100‑1 (MQ=255)
                                                                                               gcgcATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGt      <  1:1224639/100‑1 (MQ=255)
                                                                                                  cATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCg       <  1:4052866/96‑1 (MQ=255)
                                                                                                   aTGGTATTTCAGCAACGACGCTTCGGGCTGCATTCCGGCAGGTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTAc    <  1:1872400/98‑1 (MQ=255)
                                                                                                   |                                                                                                   
CGAACGTTTTGCTTTATCCGCAGAGATAACAACACTTGGCAAGCAATGGCAGGAGAAATTTGCCGCGCCGTTGATCGTTCGTCTCGATAAAGAAGGCGCATGGTATTTCAGCAACGACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTT  >  NC_000913/2181725‑2181923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑