Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,221,689 G→A 45.3% R101C (CGC→TGC)  bglX ← beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,221,6890GA45.3% 50.9 / 185.6 151R101C (CGC→TGC) bglXbeta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (37/45);  new base A (48/20);  total (85/66)
Fisher's exact test for biased strand distribution p-value = 2.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01

Only 100 of 161 total aligned reads displayed.

CATCGAGGTTAAAAGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATCATCTCGCGGAT  >  NC_000913/2221590‑2221788
                                                                                                   |                                                                                                   
  tCGAGGTTAAAAGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAggcggc                                                                                                   <  2:371676/100‑1 (MQ=255)
  tCGAGGTTAAAAGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAggcagc                                                                                                   >  1:1101237/1‑100 (MQ=255)
    gAGGTTAAAATACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCt                                                                                                  <  1:746023/99‑1 (MQ=255)
    gAGGTTAAAAGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCtt                                                                                                 >  2:383900/1‑100 (MQ=255)
    gAGGTTAAAAGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCtt                                                                                                 >  2:73427/1‑100 (MQ=255)
      ggTTAAAAGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTaa                                                                                               >  2:2223793/1‑100 (MQ=255)
        ttAAAAGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTact                                                                                              >  1:92500/1‑97 (MQ=255)
         tAAAAGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGACAAGAGGAATTTTCAGGCGGCTTAATTc                                                                                            >  1:1366415/1‑100 (MQ=255)
         tAAAAGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTc                                                                                            >  1:3132899/1‑100 (MQ=255)
           aaaGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCa                                                                                          >  2:1841099/1‑100 (MQ=255)
           aaaGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCa                                                                                          <  1:564405/100‑1 (MQ=255)
           aaaGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCa                                                                                          <  1:257172/100‑1 (MQ=255)
           aaaGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCa                                                                                          >  2:3065725/1‑100 (MQ=255)
           aaaGAAAAGGCCACACCCCGGCTAATCCGGAACACCGTCCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCa                                                                                          <  1:114977/100‑1 (MQ=255)
             aGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATc                                                                                        <  1:251933/100‑1 (MQ=255)
              gACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCa                                                                                       >  2:1586723/1‑100 (MQ=255)
                    gCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGAt                                                                                 >  2:942489/1‑100 (MQ=255)
                      cAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATcc                                                                               >  2:551216/1‑100 (MQ=255)
                      cAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATcc                                                                               <  2:755231/100‑1 (MQ=255)
                      cAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATcc                                                                               <  1:3833219/100‑1 (MQ=255)
                      cAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATcc                                                                               >  2:1276670/1‑100 (MQ=255)
                         aCCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGc                                                                            <  1:3587733/100‑1 (MQ=255)
                          ccGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCa                                                                           <  2:3338575/100‑1 (MQ=255)
                           tctggCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCAt                                                                          <  1:2686609/97‑1 (MQ=255)
                               gCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGgcg                                                                      >  1:2345625/1‑100 (MQ=255)
                                cTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGgcgc                                                                     >  2:3784642/1‑100 (MQ=255)
                                cTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGccgc                                                                     >  1:1732348/1‑100 (MQ=255)
                                   aTCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGa                                                                  <  1:438815/100‑1 (MQ=255)
                                   aTCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGa                                                                  >  1:3456207/1‑100 (MQ=255)
                                   aTCGGGAACACCGTGCGCCGACCGAGCAGCACGTCGTACGCAAAGAAAAGAGGATTTTTCCGGCGTCTTAATTCCATCACCTGATCCTGCATGGCGCGGa                                                                  <  2:3437046/100‑1 (MQ=255)
                                      gggAACACCGTGCGCTGACCGTGCAGCACGTCGGAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGat                                                                 >  1:1324507/1‑98 (MQ=255)
                                       ggAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATc                                                              <  2:3888705/100‑1 (MQ=255)
                                        gAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATcc                                                             <  2:2676414/100‑1 (MQ=255)
                                        gAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATcc                                                             >  1:2113946/1‑100 (MQ=255)
                                        gAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCAGATATcc                                                             <  2:2287522/100‑1 (MQ=255)
                                         aaCACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCt                                                            <  2:2448204/100‑1 (MQ=255)
                                          acacCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTg                                                           >  2:498518/1‑100 (MQ=255)
                                          acacCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTg                                                           >  1:152349/1‑100 (MQ=255)
                                            acCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGAc                                                         >  1:3455716/1‑100 (MQ=255)
                                            acCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGAc                                                         >  2:1791307/1‑100 (MQ=255)
                                             ccGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACg                                                        >  1:1216649/1‑100 (MQ=255)
                                               gTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACggg                                                      >  2:2394127/1‑100 (MQ=255)
                                                tGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGt                                                     <  2:3395671/100‑1 (MQ=255)
                                                tGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGt                                                     <  2:4092457/100‑1 (MQ=255)
                                                tGCGCGGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGCTCCTGCATGGCGCGGATATCCTGa                                                          <  1:2827280/95‑1 (MQ=255)
                                                  cgcTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTa                                                   <  1:1880575/100‑1 (MQ=255)
                                                     tGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACgg                                                <  1:2752377/100‑1 (MQ=255)
                                                      gACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGgt                                               >  2:4128345/1‑100 (MQ=255)
                                                      gACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGgt                                               >  2:163920/1‑100 (MQ=255)
                                                      gACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATCTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGgt                                               <  2:848973/100‑1 (MQ=255)
                                                       aCCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGCTCCTGCATGGCGCGGATATCCTGACGGGTTACGgtg                                              <  2:3012749/100‑1 (MQ=255)
                                                        ccGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGgtgt                                             <  1:121857/100‑1 (MQ=255)
                                                        ccGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGgtgt                                             >  1:2468841/1‑100 (MQ=255)
                                                          gTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTg                                           <  1:874048/100‑1 (MQ=255)
                                                          gTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTg                                           >  2:1674609/1‑100 (MQ=255)
                                                          gTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTg                                           <  1:3784625/100‑1 (MQ=255)
                                                           tGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGa                                          >  2:2695032/1‑100 (MQ=255)
                                                           tGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGa                                          >  2:209205/1‑100 (MQ=255)
                                                           tGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGa                                          <  1:1504256/100‑1 (MQ=255)
                                                              agcaCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGaaaa                                       >  2:1486304/1‑100 (MQ=255)
                                                               gcaCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAAt                                      >  2:3073745/1‑100 (MQ=255)
                                                               gcaCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAAt                                      >  2:3218814/1‑100 (MQ=255)
                                                                 aCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCg                                    <  2:359287/100‑1 (MQ=255)
                                                                 aCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCg                                    >  1:4160850/1‑100 (MQ=255)
                                                                 aCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCg                                    >  2:2501487/1‑100 (MQ=255)
                                                                   gtcgtAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGcc                                  <  2:3152041/100‑1 (MQ=255)
                                                                    tcgtAAGCAAAGACAAGAGGAATTTGCCGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGcc                                  <  1:1064126/99‑1 (MQ=255)
                                                                    tcgtAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGccc                                 <  2:1345267/100‑1 (MQ=255)
                                                                    tcgtAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGccc                                 <  2:1872232/100‑1 (MQ=255)
                                                                    tcgtAAGCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGccc                                 <  2:900764/100‑1 (MQ=255)
                                                                     cgtAAGCAAAGAAAAGAGGAATGTGCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGccc                                 <  1:1628875/99‑1 (MQ=255)
                                                                         aGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAAcc                            <  2:716995/100‑1 (MQ=255)
                                                                         aGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAAcc                            <  1:3377017/100‑1 (MQ=255)
                                                                          gCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCt                           >  1:3579687/1‑100 (MQ=255)
                                                                          gCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCt                           >  2:1615790/1‑100 (MQ=255)
                                                                          gCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCt                           >  2:3084778/1‑100 (MQ=255)
                                                                          gCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCt                           >  2:436017/1‑100 (MQ=255)
                                                                          gCAAAGAAAAGAGGAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCt                           >  1:3262838/1‑100 (MQ=255)
                                                                            aaagaaaagaGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGa                         >  1:1598895/1‑100 (MQ=255)
                                                                             aagaaaagaGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGAc                        >  2:302704/1‑100 (MQ=255)
                                                                                  aagaGGAATTTTCAGTTAGCTTAATTCCATCACCTGATCCTCCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCt                   <  1:3017922/100‑1 (MQ=255)
                                                                                   agagGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAAccagatcggaag                  >  2:1209635/1‑90 (MQ=255)
                                                                                   agagGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCtt                  >  2:95414/1‑100 (MQ=255)
                                                                                    gagGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCttt                 >  1:3888249/1‑100 (MQ=255)
                                                                                     agGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTg                <  2:2556889/100‑1 (MQ=255)
                                                                                      ggAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGa               >  2:2916520/1‑100 (MQ=255)
                                                                                       gAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGat              <  2:715945/100‑1 (MQ=255)
                                                                                       gAATTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGat              <  1:3021671/100‑1 (MQ=255)
                                                                                        aaatatCAGGCAACTTAATTCCATAACCTGATCCTGCATGGAGCGGATACCCAGACCGGATACAGTGTTGAAAAACGCCAAACCCTAAACGACTTTGata             >  1:1262739/6‑99 (MQ=255)
                                                                                        aaTTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGatc             <  1:2311315/100‑1 (MQ=255)
                                                                                        aaTTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGatc             <  1:1347877/100‑1 (MQ=255)
                                                                                        aaTTTTACGGCCGCTTCATTCCACCACCTTATCCTGCAAGGCGCGGATATCCTGACTGTTTACGCTGTTGAAAACCGACCCAACCTGACAGTCTTTCatc             <  1:1293872/100‑1 (MQ=255)
                                                                                         aTTTTCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGatca            >  2:2336182/1‑100 (MQ=255)
                                                                                            ttCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATCAtct         <  1:1032041/100‑1 (MQ=255)
                                                                                             tCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATCAtctc        <  1:1937508/100‑1 (MQ=255)
                                                                                             tCAGGCAGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATCAtctc        >  2:140073/1‑100 (MQ=255)
                                                                                                ggcggcTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATCATCTcgcg     >  2:2114495/1‑100 (MQ=255)
                                                                                                ggcagcTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATCATCTcgcg     >  2:3100355/1‑100 (MQ=255)
                                                                                                  cggcTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATCATCTCGCGGa   <  1:110628/100‑1 (MQ=255)
                                                                                                   ggcTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATCATCTCGCGGAt  >  2:3967840/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CATCGAGGTTAAAAGACGAGGCCAGACCGAGGCTAATCGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATCATCTCGCGGAT  >  NC_000913/2221590‑2221788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: