Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,229,392 A→G 41.1% intergenic (+327/+46) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,229,3920AG41.1% 71.1 / 162.0 144intergenic (+327/+46)yohP/dusCuncharacterized protein/tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base A (38/46);  new base G (25/34);  total (64/80)
Fisher's exact test for biased strand distribution p-value = 8.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 147 total aligned reads displayed.

CCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATCAGGGGAATT  >  NC_000913/2229295‑2229491
                                                                                                 |                                                                                                   
ccTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGtt                                                                                                   <  2:2929330/100‑1 (MQ=255)
ccTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGtt                                                                                                   <  2:1413028/100‑1 (MQ=255)
ccTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGtt                                                                                                   <  1:1660418/100‑1 (MQ=255)
ccTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTAtt                                                                                                   <  1:440282/100‑1 (MQ=255)
ccTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGACAATTTTGACGCCACCAAACAACCGTGCGGCCTAtt                                                                                                   >  2:2999396/1‑100 (MQ=255)
 cTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTg                                                                                                  >  2:185400/1‑100 (MQ=255)
 cTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTg                                                                                                  >  1:1360698/1‑100 (MQ=255)
  tGTAAAACGCGCCCTCAGCCCACATCCATGTGTGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGa                                                                                                 <  1:1839304/100‑1 (MQ=255)
   gTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGAt                                                                                                <  2:3417015/100‑1 (MQ=255)
    tAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATa                                                                                               <  1:3809276/100‑1 (MQ=255)
    tAAAACGCGCCCTCACCCCACATCCATGTGGGCTACCCCGGCCTTCAGTGAACGCCTCGTCAATTTTGACGCCACCAAACAACCCTGCGGCCTGCTGATa                                                                                               >  1:3403190/1‑100 (MQ=17)
    tAAAACGAGTTCTCATCCCAGATTCATGTGTGCTGCCCCTGCCTTCAGGGAACGCCTCAGCAATTTTAACGTCACTAAACATCCGTGCGGCATGTTGATa                                                                                               <  1:3315281/100‑1 (MQ=12)
     aaaaCGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAGCGCCTCGTCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATaa                                                                                              <  1:2986333/100‑1 (MQ=255)
     aaaaCGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATaa                                                                                              <  1:575970/100‑1 (MQ=255)
      aaaCGCGCCCTCAGCCCACATCCATGTGGGCCGCCCCGGCCTTCAGGGCACGCCTCGGCAATTCTGAAGCCACCCAACAACCGTGCGGCCTCTTGATaca                                                                                             >  1:2724624/1‑98 (MQ=17)
       aaCGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAag                                                                                            >  1:1583348/1‑100 (MQ=255)
        aCGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAaga                                                                                           <  1:640980/100‑1 (MQ=255)
        aCGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAaga                                                                                           <  1:2858708/100‑1 (MQ=255)
         cgcgCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAagag                                                                                          >  1:3056156/1‑100 (MQ=255)
         cgcgCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAagag                                                                                          <  1:675944/100‑1 (MQ=255)
         cgcgCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAagag                                                                                          >  2:1631290/1‑100 (MQ=255)
          gcgcCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGc                                                                                         <  1:1765596/100‑1 (MQ=255)
          gcgcCCGCAGCCTACATCCATGTGGGCTGCCCCGGCCTTCGGGGACCGCCTCGGCAGTTTTGCCGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGc                                                                                         >  1:3568640/1‑100 (MQ=21)
           cgcCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTGAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCt                                                                                        >  1:2640955/1‑100 (MQ=255)
           ccggggcAGCCCACATGGATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCt                                                                                        >  2:3472090/7‑100 (MQ=21)
            gcCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTa                                                                                       <  2:1155021/100‑1 (MQ=255)
            gcCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTa                                                                                       <  1:762809/100‑1 (MQ=255)
            gcCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTa                                                                                       <  1:1086770/100‑1 (MQ=255)
              gggcAGCCCACATGGATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAac                                                                                     >  2:2154550/4‑100 (MQ=21)
               cTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAaca                                                                                    <  1:165225/100‑1 (MQ=255)
               cTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAaca                                                                                    <  2:1173598/100‑1 (MQ=255)
               cTCAGCCCACATCCATGTGGGCTGCCCCGGCCTCCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAaca                                                                                    <  1:3877409/100‑1 (MQ=255)
                gcAGCCCACATGGATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACACTCGTGCGGCCTATTGATAAAGAGCTAacac                                                                                   >  2:353380/2‑100 (MQ=21)
                 cAGCCCACATGGATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAAcaca                                                                                  >  2:2270250/1‑100 (MQ=255)
                 cAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAAcaca                                                                                  <  2:1576321/100‑1 (MQ=255)
                 cAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAAcaca                                                                                  >  1:3537420/1‑100 (MQ=255)
                  aGCCCACATGGCTGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCTTGCGGCCTGTTGATAAAGAGCTAACACAt                                                                                 >  1:2046693/1‑100 (MQ=21)
                  aGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACAt                                                                                 >  1:1161156/1‑100 (MQ=255)
                  aGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACAt                                                                                 <  2:4226512/100‑1 (MQ=255)
                    cccACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTg                                                                               <  2:2692460/100‑1 (MQ=255)
                     ccACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGt                                                                              <  1:1269496/100‑1 (MQ=255)
                     ccACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCCGTTGATAAAGAGCTAACACATTGt                                                                              >  2:2210090/1‑100 (MQ=255)
                      cacaTCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTc                                                                             >  2:1583502/1‑100 (MQ=255)
                       acaTCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCa                                                                            <  2:194717/100‑1 (MQ=255)
                       acaTCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCa                                                                            <  2:812825/100‑1 (MQ=255)
                       acaTCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGCGCGGCCTATTGATAAAGTGCTAACACAtttt                                                                              <  2:2877933/98‑3 (MQ=255)
                          tggaTGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCaaaa                                                                         >  1:3367997/4‑100 (MQ=255)
                            cATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCaaaaaa                                                                       >  2:1010196/1‑100 (MQ=255)
                            cATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCaaaaaa                                                                       <  1:3035845/100‑1 (MQ=255)
                            cATCTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCCATTTTGACGCCACCTAACAACCGTGCGGCCTGTTGATAACGAGCTAACACATTGTCaaaaa                                                                        >  1:2890060/1‑99 (MQ=25)
                               gtgGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACAt                                                                    >  2:2096553/1‑100 (MQ=255)
                               gtgGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCAGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACAt                                                                    >  1:1429477/1‑100 (MQ=255)
                                tgGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATc                                                                   >  1:3421593/1‑100 (MQ=255)
                                tgGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATc                                                                   <  2:322399/100‑1 (MQ=255)
                                 gggCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCa                                                                  >  2:2736661/1‑100 (MQ=255)
                                 gggCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCa                                                                  >  2:1251111/1‑100 (MQ=255)
                                  ggCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCAc                                                                 >  1:2427775/1‑100 (MQ=255)
                                  ggCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCAc                                                                 <  1:2342104/100‑1 (MQ=255)
                                           ggCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGAGAAAGAGCTAACACATTGTCAAAAAACATCACTATGGtttt                                                        <  2:2122491/100‑1 (MQ=255)
                                             ccTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGtttttt                                                      <  1:3837365/100‑1 (MQ=255)
                                              cTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTa                                                     >  2:700219/1‑100 (MQ=255)
                                              cTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTa                                                     >  2:2469328/1‑100 (MQ=255)
                                                tCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTaga                                                   <  2:3588484/100‑1 (MQ=255)
                                                tCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTaga                                                   <  2:879702/100‑1 (MQ=255)
                                                 cAGGTAACGCCTCGGCAATTTTGACGCCACCAAACACCCGTGCGGCCTATTGATAAATAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTagag                                                  <  1:2056093/100‑1 (MQ=255)
                                                 cAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTagan                                                  <  1:1493447/100‑2 (MQ=255)
                                                 cAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTagag                                                  >  2:1495026/1‑100 (MQ=255)
                                                  aGGGAACGCCTCGGTCATTTTACCGCCACCAAACAACATTGCGGCCTGTTGATAATTAGCTAAAAAAAAATAAAAAAACATCACTATGGtttttt                                                      <  1:812130/95‑1 (MQ=255)
                                                   gggAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGtt                                                >  1:3687102/1‑100 (MQ=255)
                                                        cGCCTCGTCTATTTTGACGCCACCCAACTACCCTGCTGCCTATTGATGAACAGCTAACACATTGTCACCAACCTTCAGGATGGTTTTTTAGGGTTtc                                              <  1:1778042/97‑1 (MQ=255)
                                                        cGCCTCGGCAATTTTGACGCCACCAACCAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCg                                           <  2:220351/100‑1 (MQ=255)
                                                        cGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCg                                           <  1:2401752/100‑1 (MQ=255)
                                                         gCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGa                                          >  2:2322610/1‑100 (MQ=255)
                                                          ccTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGCTAAAGAGCTAACAAATTGTCAAAAAACATCACTATGGTTCTTTAGAGGTTCTCGat                                         >  1:2471669/1‑100 (MQ=255)
                                                            tCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGatat                                       <  2:4227989/100‑1 (MQ=255)
                                                             cGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCTAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATc                                      <  1:2621551/100‑1 (MQ=255)
                                                                cAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAAt                                   <  2:3614345/100‑1 (MQ=255)
                                                                   ttttGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGCTTCTTAGAGTTTCTCGATATCAATTGc                                >  1:3301376/1‑100 (MQ=255)
                                                                    tttGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGcc                               <  2:3930943/100‑1 (MQ=255)
                                                                      tGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTg                             <  1:4166144/100‑1 (MQ=255)
                                                                      tGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTg                             <  1:1182564/100‑1 (MQ=255)
                                                                         cGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAAt                          >  2:386557/1‑100 (MQ=255)
                                                                            caccaAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGc                       <  1:1140284/100‑1 (MQ=255)
                                                                              ccaAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTCTTTAGAGTTTCTCGATATCAATTGCCTGAATAGccc                     >  1:2836142/1‑100 (MQ=255)
                                                                               caAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCt                    >  2:3290578/1‑100 (MQ=255)
                                                                               caAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCt                    <  2:3343502/100‑1 (MQ=255)
                                                                               caAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCt                    >  1:1580390/1‑100 (MQ=255)
                                                                                aaaCAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCtt                   >  2:4080801/1‑100 (MQ=255)
                                                                                aaaCAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCtt                   <  2:245647/100‑1 (MQ=255)
                                                                                  acaacCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGc                 <  2:4144185/100‑1 (MQ=255)
                                                                                  acaacCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGc                 <  1:3019805/100‑1 (MQ=255)
                                                                                  acaacCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGc                 <  1:2787140/100‑1 (MQ=255)
                                                                                   caacCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCTCTTGCa                >  1:2946636/1‑100 (MQ=255)
                                                                                    aacCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCaa               >  2:680760/1‑100 (MQ=255)
                                                                                        gTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATc           >  1:2821854/1‑100 (MQ=255)
                                                                                         tGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATCa          <  2:3214751/100‑1 (MQ=255)
                                                                                            ggCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATCAggg       <  1:1116607/100‑1 (MQ=255)
                                                                                                tATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATCAGGGGAat   >  1:3178676/1‑100 (MQ=255)
                                                                                                 aTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATCAGGGGAatt  >  2:2625722/1‑100 (MQ=255)
                                                                                                 aTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCACTTGCAATATCAGGGTAat   >  2:1743836/1‑99 (MQ=255)
                                                                                                 |                                                                                                   
CCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATCAGGGGAATT  >  NC_000913/2229295‑2229491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: