Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,255,992 A→T 18.6% K213I (AAA→ATA) ‡ yeiL → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,255,9920AT18.6% 441.2 / 88.0 187K213I (AAA→ATA) yeiLputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (75/77);  new base T (24/11);  total (99/88)
Fisher's exact test for biased strand distribution p-value = 5.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

Only 100 of 201 total aligned reads displayed.

TCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCC  >  NC_000913/2255893‑2256091
                                                                                                   |                                                                                                   
tcattcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaa                                                                                                     >  2:2751279/1‑100 (MQ=255)
  attcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAAt                                                                                                   >  1:3504116/1‑100 (MQ=255)
     tcttccgatctattaATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATtct                                                                                                <  2:2961266/90‑1 (MQ=255)
     caCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaagatcg                                                                                                >  1:103932/1‑95 (MQ=255)
     caCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATtct                                                                                                >  1:241446/1‑100 (MQ=255)
       cgacgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTcc                                                                                              <  1:2186611/100‑1 (MQ=255)
       cgacgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTcc                                                                                              <  2:2696516/100‑1 (MQ=255)
        gacgGTTTATGAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGACGCTGGAGATGGACCCGGAGAATTTATTCTCCg                                                                                             <  1:2798509/100‑1 (MQ=255)
          cgGTTTATTAATTAACAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCggg                                                                                           <  2:4206913/100‑1 (MQ=255)
          cgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCggg                                                                                           <  2:393414/100‑1 (MQ=255)
          cgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCggg                                                                                           <  2:1733841/100‑1 (MQ=255)
          cgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCggg                                                                                           <  2:687095/100‑1 (MQ=255)
             tttATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgatg                                                                                        >  2:2204742/1‑100 (MQ=255)
             tttATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgatg                                                                                        >  1:1095175/1‑100 (MQ=255)
              ttattaATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgggtcca                                                                                       <  2:4212252/100‑5 (MQ=39)
               tattaATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgatgat                                                                                      >  2:3117534/1‑100 (MQ=255)
                attaattaAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGatgatg                                                                                     <  1:1170522/100‑1 (MQ=255)
                 ttaattaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGACGGACCCGGAGAATAAATTCTCCGGGATGATGc                                                                                    <  1:227435/100‑1 (MQ=255)
                   aattaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgggtccatctcc                                                                                  <  2:2053441/100‑10 (MQ=38)
                    attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGt                                                                                 >  2:944288/1‑100 (MQ=255)
                    attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGt                                                                                 >  2:2918451/1‑100 (MQ=255)
                    attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGt                                                                                 >  2:3864858/1‑100 (MQ=255)
                    attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGt                                                                                 >  2:4176392/1‑100 (MQ=255)
                    attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGt                                                                                 >  2:1222348/1‑100 (MQ=255)
                    attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGt                                                                                 <  1:992056/100‑1 (MQ=255)
                    attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGt                                                                                 >  1:1581300/1‑100 (MQ=255)
                    attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACACGGAGAATAAATTCTCCGGGATGATGCAGt                                                                                 >  1:2844156/1‑100 (MQ=255)
                      taaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTaa                                                                               >  2:766757/1‑100 (MQ=255)
                      taaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTaa                                                                               >  1:1174388/1‑100 (MQ=255)
                         aaGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTaaaaa                                                                            >  1:3120414/1‑100 (MQ=255)
                         aaGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTaaaaa                                                                            >  1:3552521/1‑100 (MQ=255)
                            cAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAatta                                                                         >  1:1375762/1‑100 (MQ=255)
                            cAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAaata                                                                         >  1:1670191/1‑100 (MQ=255)
                               gAAAGGTTATCTCATTAAAAACAGAAATCAGTTGTCAGGACTGGCGCTGGAGATTGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTAttt                                                                      <  1:828918/100‑1 (MQ=255)
                                 aaGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc                                                                    <  2:3729146/100‑1 (MQ=255)
                                 aaGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc                                                                    >  2:1196700/1‑100 (MQ=255)
                                    ggTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAAt                                                                 <  2:2552906/100‑1 (MQ=255)
                                        tctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAgcg                                                             >  2:189332/1‑100 (MQ=255)
                                         ctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTACGGGAGGCTGCAGTAAACATTATGTTTAATAgcg                                                             >  1:4106814/1‑99 (MQ=255)
                                          tcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGcgcg                                                           >  1:723741/1‑100 (MQ=255)
                                             ttAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGAtt                                                        >  2:2230689/1‑100 (MQ=255)
                                              tAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTg                                                       >  1:3874601/1‑100 (MQ=255)
                                              tAAAAACAGAAAGCAGTTGTCAGGACGGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTg                                                       <  1:2715321/100‑1 (MQ=255)
                                                 aaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCg                                                    <  2:2388357/100‑1 (MQ=255)
                                                     aGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGAt                                                 <  1:3075230/99‑1 (MQ=255)
                                                     aGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATg                                                <  1:4054695/100‑1 (MQ=255)
                                                      gAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGc                                               >  1:459385/1‑100 (MQ=255)
                                                      gAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGc                                               >  1:3739864/1‑100 (MQ=255)
                                                      gAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGc                                               <  2:1448570/100‑1 (MQ=255)
                                                      gAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGc                                               <  2:1240874/100‑1 (MQ=255)
                                                      gAAAGCAGTTGTCAGAACTGGCGCTGGAGATGTACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGc                                               <  2:4012461/100‑1 (MQ=255)
                                                          gCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACg                                           >  2:4115056/1‑100 (MQ=255)
                                                             gTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGTAACGCtt                                        <  2:3783196/100‑1 (MQ=255)
                                                              ttGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTa                                       <  1:3029833/100‑1 (MQ=255)
                                                               tGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTaa                                      <  1:4251980/100‑1 (MQ=255)
                                                                gTCAGGACTGGCGCTGGCGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAACTTATTTTCAATAGCGAGATTGCCGGATCCAACGCTTAAt                                     >  1:3890644/1‑100 (MQ=255)
                                                                gTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAAt                                     <  2:1838333/100‑1 (MQ=255)
                                                                 tCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATa                                    <  2:578005/100‑1 (MQ=255)
                                                                 tCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATa                                    >  2:1975696/1‑100 (MQ=255)
                                                                  cAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATAc                                   >  2:2607623/1‑100 (MQ=255)
                                                                    ggACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGt                                 <  2:2136184/100‑1 (MQ=255)
                                                                    ggACCGGCGCTGGAGACGGACCCGGAGAATAAATTTTCCGGCATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGt                                 <  2:1771971/100‑1 (MQ=255)
                                                                      aCTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGttt                               <  2:15119/100‑1 (MQ=255)
                                                                      aCTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGttt                               <  1:4274910/100‑1 (MQ=255)
                                                                       cTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGTTGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGTATGCAACGCTTAATACGtt                                <  2:2680371/98‑1 (MQ=255)
                                                                       cTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtttt                              >  1:597505/1‑100 (MQ=255)
                                                                         ggCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTAt                            >  2:1228245/1‑100 (MQ=255)
                                                                          gcgcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATc                           <  2:3998884/100‑1 (MQ=255)
                                                                           cgcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATcc                          >  2:3584534/1‑100 (MQ=255)
                                                                           cgcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATcc                          >  2:198742/1‑100 (MQ=255)
                                                                             cTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCgg                        <  1:1244289/100‑1 (MQ=255)
                                                                             cTGGAGACGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAACACGTTTTATCCgg                        <  1:2675180/100‑1 (MQ=255)
                                                                               ggAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCTGGATGCAACGCTTAATATGTTTTATCCGGTc                      <  2:2873930/100‑1 (MQ=255)
                                                                               ggAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTc                      <  2:335751/100‑1 (MQ=255)
                                                                               ggAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTc                      <  1:1800920/100‑1 (MQ=255)
                                                                                 gcatcatcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTa                    >  1:695284/8‑100 (MQ=38)
                                                                                    tGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACaa                 >  2:2970690/1‑100 (MQ=255)
                                                                                     ggACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGCTTGCCGCATGCAACGCTTAATACGTTTTATCCGGTCTACaaa                >  2:4246816/1‑100 (MQ=255)
                                                                                     catcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACaaa                >  1:675632/4‑100 (MQ=255)
                                                                                     catcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACaaa                >  2:1489704/4‑100 (MQ=255)
                                                                                      gACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAAt               >  1:1150220/1‑100 (MQ=255)
                                                                                      gACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAAt               >  1:2526101/1‑100 (MQ=255)
                                                                                       tcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATc              >  1:2303212/2‑100 (MQ=255)
                                                                                       aCCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATc              <  2:1558212/100‑1 (MQ=255)
                                                                                        cccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATc              >  1:2369482/1‑99 (MQ=255)
                                                                                        cccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCg             >  2:1504644/1‑100 (MQ=255)
                                                                                        cccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCg             >  2:3046482/1‑100 (MQ=255)
                                                                                        cccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCg             >  1:661984/1‑100 (MQ=255)
                                                                                        cccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCg             >  1:4118095/1‑100 (MQ=255)
                                                                                         ncGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTAATGTCATCATCGCGATTGCGGGATGCAACACTTATTACGTTTTATCCGGTCGACa                  >  1:2089821/2‑94 (MQ=255)
                                                                                         ccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa            >  2:1620365/1‑100 (MQ=255)
                                                                                          cGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAg           <  2:1188300/100‑1 (MQ=255)
                                                                                            gagaATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGc          <  1:2431787/99‑1 (MQ=255)
                                                                                               aaTAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTa      >  2:3475234/1‑100 (MQ=255)
                                                                                                aTAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTAc     >  1:4071077/1‑100 (MQ=255)
                                                                                                  aaaTTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGc   >  1:1876857/1‑100 (MQ=255)
                                                                                                  aaaTTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGc   <  2:537725/100‑1 (MQ=255)
                                                                                                   aaTTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGcc  <  2:3428003/100‑1 (MQ=255)
                                                                                                   aaTTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGcc  >  2:1117242/1‑100 (MQ=255)
                                                                                                   aaTTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGcc  <  2:2079784/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCC  >  NC_000913/2255893‑2256091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: