Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,269,044 T→C 40.0% F64S (TTC→TCC)  lpxT → lipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,269,0440TC40.0% 93.0 / 156.1 142F64S (TTC→TCC) lpxTlipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (39/46);  new base C (26/31);  total (65/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 146 total aligned reads displayed.

GGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGG  >  NC_000913/2268945‑2269142
                                                                                                   |                                                                                                  
ggTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTTTTTTTTTTTTCATTGACCAACAATCGCGCCtt                                                                                                    >  1:2500034/1‑100 (MQ=255)
ggTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCtt                                                                                                    >  1:304815/1‑100 (MQ=255)
 gTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTc                                                                                                   >  2:1886652/1‑100 (MQ=255)
 gTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTc                                                                                                   <  2:3911980/100‑1 (MQ=255)
    gCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGAc                                                                                                >  2:2458798/1‑100 (MQ=255)
    gCCGATTGATGCGGATAGTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGAc                                                                                                <  2:2170308/100‑1 (MQ=255)
     ccGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACg                                                                                               >  2:1062416/1‑100 (MQ=255)
         ttGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGgttg                                                                                           <  1:3377229/100‑1 (MQ=255)
         ttGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGgttg                                                                                           <  2:2184209/100‑1 (MQ=255)
         ttGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGgttg                                                                                           <  1:1138220/100‑1 (MQ=255)
           gATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGgttgtt                                                                                         <  2:2148697/100‑1 (MQ=255)
            aTGCGGTTATTTTTTATTTCTTTAAGCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTTGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTc                                                                                        <  1:861368/100‑1 (MQ=255)
            aTGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTc                                                                                        <  2:1991584/100‑1 (MQ=255)
            aTGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTc                                                                                        >  2:3617999/1‑100 (MQ=255)
              gCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCAc                                                                                      <  1:1983787/100‑1 (MQ=255)
              gCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCAc                                                                                      >  2:3788128/1‑100 (MQ=255)
                ggATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCActg                                                                                    >  2:1014778/1‑100 (MQ=255)
                 gATATTTTTTATTTCTTTAATCAGAAGCTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCActgc                                                                                   <  2:1842650/100‑1 (MQ=255)
                    atTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTgg                                                                                <  1:2184524/100‑1 (MQ=255)
                     ttttttATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGc                                                                               <  1:2974961/100‑1 (MQ=255)
                      tttttATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGGTCACTGCTGGCg                                                                              <  2:2807637/100‑1 (MQ=255)
                       ttttATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGa                                                                             <  2:2413641/100‑1 (MQ=255)
                        tttATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGAt                                                                            <  2:166393/100‑1 (MQ=255)
                        tttATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGAt                                                                            >  2:2115313/1‑100 (MQ=255)
                          tATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATgg                                                                          <  1:3484899/100‑1 (MQ=255)
                          tATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATgg                                                                          <  1:3484035/100‑1 (MQ=255)
                          tATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATgg                                                                          >  1:2104933/1‑100 (MQ=255)
                           aTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATggg                                                                         >  1:2716433/1‑100 (MQ=255)
                           aTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATggg                                                                         <  2:739735/100‑1 (MQ=255)
                            tttCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGt                                                                        >  2:2111098/1‑100 (MQ=255)
                             ttCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTa                                                                       >  2:352256/1‑100 (MQ=255)
                              tCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTAt                                                                      <  1:700373/100‑1 (MQ=255)
                               cTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTTTTTTTTTTTTTATTGACCAACAATCGCGCCTTCGACGGTTGTTTACTGCTGGCGATGGGTATg                                                                     >  1:1527448/1‑100 (MQ=255)
                               cTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATg                                                                     >  2:2202457/1‑100 (MQ=255)
                                 ttAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTTTTTTTGTTTTCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGt                                                                    >  1:2520850/1‑99 (MQ=255)
                                  tAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTg                                                                  >  2:1171099/1‑100 (MQ=255)
                                   aaTCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGa                                                                 <  1:2458792/100‑1 (MQ=255)
                                   aaTCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGa                                                                 <  1:4175554/100‑1 (MQ=255)
                                     tCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATg                                                               <  1:1824098/100‑1 (MQ=255)
                                      cAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATg                                                               >  2:316080/1‑99 (MQ=255)
                                      cAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGc                                                              <  2:1648751/100‑1 (MQ=255)
                                      cAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGc                                                              <  1:3126266/100‑1 (MQ=255)
                                      cAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGc                                                              >  2:3746633/1‑100 (MQ=255)
                                       aGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCt                                                             >  1:2690102/1‑100 (MQ=255)
                                       aGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCt                                                             >  1:292071/1‑100 (MQ=255)
                                        gAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTg                                                            >  1:2792978/1‑100 (MQ=255)
                                        gAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTg                                                            <  2:4033344/100‑1 (MQ=255)
                                          aaCTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAg                                                          <  1:3687193/100‑1 (MQ=255)
                                              ggTCGAAAGTAAGGCCTTTTTTTTTTTTTTTTCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGCTGCTGAGGTTc                                                      >  1:4277916/1‑100 (MQ=255)
                                              ggTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTc                                                      >  2:3586922/1‑100 (MQ=255)
                                                tCGAAAGTAAGGCCTTTTTGTGGCTAGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTg                                                    >  2:744472/1‑100 (MQ=255)
                                                 cGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTgg                                                   >  2:3397637/1‑100 (MQ=255)
                                                 cGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTgg                                                   <  1:1892836/100‑1 (MQ=255)
                                                 cGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTgg                                                   >  1:1230166/1‑100 (MQ=255)
                                                 cGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTgg                                                   >  1:3037354/1‑100 (MQ=255)
                                                  gAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGc                                                  <  2:1026674/100‑1 (MQ=255)
                                                  gAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGc                                                  <  2:3221667/100‑1 (MQ=255)
                                                   aaaGTAAGGCCTTTTTGTGGCTGGTTGCATTGACGAACAATCCCGCTTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCt                                                 <  1:3001985/100‑1 (MQ=255)
                                                   aaaGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCt                                                 <  2:476266/100‑1 (MQ=255)
                                                   aaaGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCt                                                 <  2:841706/100‑1 (MQ=255)
                                                   aaaGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCt                                                 <  1:362745/100‑1 (MQ=255)
                                                    aaGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTg                                                <  1:534034/100‑1 (MQ=255)
                                                     aGTAAGGCCTTTTTTTTTTTTTTTTCATTGACCAACAACCGCGCCTTTGACGGTTGTTCACTGCTGGCGATGGGTCTGTTGTTGCTGAGTTTCTGGCt                                                 >  1:1762374/1‑98 (MQ=255)
                                                      gTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCTCCTCCGACTGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaa                                              <  1:884926/100‑1 (MQ=255)
                                                      gTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaa                                              >  2:853120/1‑100 (MQ=255)
                                                        aaGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaaag                                            <  2:584083/100‑1 (MQ=255)
                                                        aaGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaaag                                            <  2:4250720/100‑1 (MQ=255)
                                                        aaGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaaag                                            >  2:4264915/1‑100 (MQ=255)
                                                          ggCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaaagaa                                          >  2:3961046/1‑100 (MQ=255)
                                                          ggCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaaagaa                                          >  2:683380/1‑100 (MQ=255)
                                                           gCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaaagaaa                                         >  1:567507/1‑100 (MQ=255)
                                                            ccTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGaaaa                                        <  1:3832626/100‑1 (MQ=255)
                                                             cTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAAc                                       <  1:3690425/100‑1 (MQ=255)
                                                                 ttGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGccc                                   <  2:2925753/100‑1 (MQ=255)
                                                                  tgtgGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGcccc                                  <  1:1062413/100‑1 (MQ=255)
                                                                      gCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGc                              <  1:1701035/100‑1 (MQ=255)
                                                                       cTGGTTGCTTTGACCAACAAGCGCGCCCTAGAGGGTTGTGGACGTCTGGCGATGGGTAGGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCt                                 <  1:4042143/96‑1 (MQ=255)
                                                                       cTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGc                              <  1:4244080/99‑1 (MQ=255)
                                                                        tGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAg                            >  2:3230816/1‑100 (MQ=255)
                                                                             gCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacgag                       >  2:633045/1‑99 (MQ=255)
                                                                             gCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacgac                       >  2:3728709/1‑100 (MQ=255)
                                                                             gCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacgac                       >  2:208467/1‑100 (MQ=255)
                                                                              cATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGacgacg                      >  2:2014219/1‑100 (MQ=255)
                                                                                ttGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTa                    <  2:52129/100‑1 (MQ=255)
                                                                                ttGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTa                    >  2:379536/1‑100 (MQ=255)
                                                                                  gACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATc                  >  1:1602571/1‑100 (MQ=255)
                                                                                   aCCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCg                 >  1:3420253/1‑100 (MQ=255)
                                                                                     caacaaTCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAACACGCCCCTGGCAGACGACGTATGGTg               >  1:3971638/1‑100 (MQ=255)
                                                                                     caacaaTCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTg               >  1:3035859/1‑100 (MQ=255)
                                                                                        caaTCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGat             <  1:3787694/99‑1 (MQ=255)
                                                                                         aaTCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGatt            >  1:761638/1‑99 (MQ=255)
                                                                                            cgcgCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtg        <  1:1883738/100‑1 (MQ=255)
                                                                                            cgcgCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtg        >  1:2354743/1‑100 (MQ=255)
                                                                                             gcgcCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtgg       >  2:223230/1‑100 (MQ=255)
                                                                                             gcgcCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtgg       <  1:1014775/100‑1 (MQ=255)
                                                                                             gcgcCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGGTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtgg       >  2:1374479/1‑100 (MQ=255)
                                                                                                ccTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtct    <  2:464612/100‑1 (MQ=255)
                                                                                                ccTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtct    >  1:1595093/1‑100 (MQ=255)
                                                                                                ccTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGAAGACGACGTATCGTGATTATtggtct    <  2:2590526/100‑1 (MQ=255)
                                                                                                   tCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtctgg  <  1:1717511/99‑1 (MQ=255)
                                                                                                   |                                                                                                  
GGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGG  >  NC_000913/2268945‑2269142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: