Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,281,279 A→T 55.0% A216A (GCA→GCT yejH → putative ATP‑dependent DNA or RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,281,2790AT55.0% 41.1 / 207.8 154A216A (GCA→GCTyejHputative ATP‑dependent DNA or RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (39/29);  new base T (40/44);  total (81/73)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

Only 100 of 159 total aligned reads displayed.

GCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGCT  >  NC_000913/2281183‑2281378
                                                                                                |                                                                                                   
gCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCag                                                                                                  <  2:3534634/100‑1 (MQ=255)
gCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCag                                                                                                  <  2:3534970/100‑1 (MQ=255)
gCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCag                                                                                                  <  2:176758/100‑1 (MQ=255)
gCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACag                                                                                                  <  2:1092816/100‑1 (MQ=255)
   gCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGt                                                                                               >  2:4240994/1‑100 (MQ=255)
   gCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGt                                                                                               >  2:706655/1‑100 (MQ=255)
   gCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGt                                                                                               >  2:526126/1‑100 (MQ=255)
     cGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTaa                                                                                             <  2:4086909/100‑1 (MQ=255)
       cTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACg                                                                                           <  2:1381992/100‑1 (MQ=255)
          cGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGc                                                                                        <  2:328583/100‑1 (MQ=255)
           gTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCt                                                                                       >  2:1561519/1‑100 (MQ=255)
             tataTGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGt                                                                                     >  2:15356/1‑100 (MQ=255)
             tataTGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGt                                                                                     <  1:2091272/100‑1 (MQ=255)
               tatGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTc                                                                                   >  1:1641952/1‑100 (MQ=255)
                 tGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAg                                                                                 >  1:2802503/1‑100 (MQ=255)
                 tGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAg                                                                                 <  1:702427/100‑1 (MQ=255)
                 tGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAg                                                                                 >  1:2221635/1‑100 (MQ=255)
                 tGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAg                                                                                 >  1:3747376/1‑100 (MQ=255)
                 tGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAg                                                                                 <  2:1146793/100‑1 (MQ=255)
                  gATTCAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGc                                                                                <  1:3263412/100‑1 (MQ=255)
                  gATTAAACACGGCTCTCTGACGCCGCTAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAg                                                                                 <  1:2315814/99‑1 (MQ=255)
                  gATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAg                                                                                 >  2:1475899/1‑99 (MQ=255)
                    ttAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTCCAGCCGCTTGCAGGCCCAGAGTAACGGGCTGTTCAGCGa                                                                              >  2:2477614/1‑100 (MQ=255)
                       aaCACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGc                                                                           >  2:3190880/1‑100 (MQ=255)
                       aaCACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGc                                                                           <  2:3763766/100‑1 (MQ=255)
                           cGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGAt                                                                       >  1:3763263/1‑100 (MQ=255)
                           cGGCTATCTGACGACGCCAGAACGACTGGATATGCCAGCAGTGCAATACGATTTCAGCCGCTTGCAGGCTAAGAGTAACGGGCTGTTCAGCGAAGCCg                                                                         >  1:2640697/1‑98 (MQ=255)
                            ggCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGAtc                                                                      <  1:839316/100‑1 (MQ=255)
                            ggCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGAtc                                                                      <  2:818882/100‑1 (MQ=255)
                            ggCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGAtc                                                                      <  2:3862437/100‑1 (MQ=255)
                            ggCTAGCTGACGCCGCAAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGAt                                                                       <  1:2032863/99‑1 (MQ=255)
                             gCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGAtct                                                                     <  1:50479/100‑1 (MQ=255)
                             gCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGAtct                                                                     <  2:1304499/100‑1 (MQ=255)
                              cTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGAtctc                                                                    >  1:312144/1‑100 (MQ=255)
                                 tCTGACGCCGCCAGAACGACTGGATATACCAGTAGTGCAGTACGATCTCAGCTGCTGGCAGGCACAGAATAACGGGCTGTTCCGCGAAGCCGATCTCAAc                                                                 >  1:1798535/1‑100 (MQ=255)
                                     aCGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTg                                                             <  2:194035/100‑1 (MQ=255)
                                       gccgccAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAg                                                           <  2:1641957/100‑1 (MQ=255)
                                        cctccAGATCGCCTTGATATTCCAGTAGTGTATTACGATGTCAGCCGCGCGCAGGCTCACAGTAACGGGCTATTCCGCGAAGCCGATCTCACCCGTGAGc                                                          <  1:1087959/97‑1 (MQ=255)
                                        ccgccAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGc                                                          >  1:1588790/1‑100 (MQ=255)
                                         cgccAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCt                                                         <  1:885132/100‑1 (MQ=255)
                                          gccAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTAAGCGAAGCCGATCTCAACCGTGAGCTg                                                        >  2:1049357/1‑100 (MQ=255)
                                          gccACAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTATCGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCt                                                         <  1:455180/99‑1 (MQ=255)
                                              gAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaaaa                                                    <  2:2067844/100‑1 (MQ=255)
                                              gAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGaaaa                                                    <  2:2547139/100‑1 (MQ=255)
                                                aCGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCGGaaaaaa                                                  <  2:2240974/100‑1 (MQ=255)
                                                 ngACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaac                                                 >  2:1534846/2‑100 (MQ=255)
                                                 cGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaac                                                 <  2:1890675/100‑1 (MQ=255)
                                                  taCTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaaca                                                <  1:3435653/99‑1 (MQ=255)
                                                  gACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaaca                                                >  2:1395210/1‑100 (MQ=255)
                                                    cTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaac                                              <  2:1679762/100‑1 (MQ=255)
                                                      ggATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaacaa                                            >  1:1620308/1‑100 (MQ=255)
                                                           tGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTAt                                       >  2:2755164/1‑100 (MQ=255)
                                                           tGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTAt                                       <  2:3916837/100‑1 (MQ=255)
                                                            gCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTAtt                                      >  1:1199550/1‑100 (MQ=255)
                                                            gCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAAAAGAAAAAAAAAAAAACAACAACGTAtt                                      <  1:3234513/100‑1 (MQ=255)
                                                              cAGTAGTGCAATAAGATTTCAGCCGCTTGCAGGCTCAGAGAAACAGGCTGTTCAGCGAGGCTGATCTTAAAAAAAAAAAAAAAAAACAACAACGTAtt                                      <  1:425215/98‑1 (MQ=255)
                                                                 tagtGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTAcccc                                 <  2:4175685/100‑1 (MQ=255)
                                                                 tagtGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTAcccc                                 <  2:900957/100‑1 (MQ=255)
                                                                 tagtGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTAcccc                                 <  2:4169642/100‑1 (MQ=255)
                                                                 tagtGCAATACGATTTCAGCCGATTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTAcccc                                 <  1:2889660/100‑1 (MQ=255)
                                                                  agtGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTAcccc                                 >  1:2627296/1‑99 (MQ=255)
                                                                   gtGCAATACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGc                               >  2:2150728/1‑100 (MQ=255)
                                                                    tGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGca                              <  1:2361544/100‑1 (MQ=255)
                                                                     gCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac                             >  2:3345640/1‑100 (MQ=255)
                                                                     gCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac                             >  2:814172/1‑100 (MQ=255)
                                                                     gCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac                             >  1:3206285/1‑100 (MQ=255)
                                                                     gCAATACGATTTCAGCCGCTTGCAGACTCATAGTAACGGGCTGTTCATCTAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcac                             >  1:1478805/1‑100 (MQ=255)
                                                                      cAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcaca                            <  2:192448/100‑1 (MQ=255)
                                                                      cAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGcaca                            <  2:2724010/100‑1 (MQ=255)
                                                                       aaTACGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCAcat                           >  2:562888/1‑100 (MQ=255)
                                                                           cGATTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACatcatc                       <  1:3283316/100‑1 (MQ=255)
                                                                             aTTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAg                     <  2:1686242/100‑1 (MQ=255)
                                                                             aTTTCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAg                     >  2:3632018/1‑100 (MQ=255)
                                                                               ttCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGcc                   >  1:924080/1‑100 (MQ=255)
                                                                               ttCAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGcc                   <  2:2640706/100‑1 (MQ=255)
                                                                                tCAGCCGCTTGCAGGCACAGAGTAACGGGCTCTTCAGCGAAGCCAATCTCAACCGTGAACGGAAAAAACAACAACGTATTACCCCGCACATCAACAGc                    >  1:1717779/1‑98 (MQ=255)
                                                                                 cAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGcc                   >  1:1444743/1‑98 (MQ=255)
                                                                                 cAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAg                 >  2:857095/1‑100 (MQ=255)
                                                                                 cAGCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAg                 >  2:933965/1‑100 (MQ=255)
                                                                                 cAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAg                 <  1:1179924/100‑1 (MQ=255)
                                                                                   gCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGAt               >  2:2035982/1‑100 (MQ=255)
                                                                                   gCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGAt               >  2:742008/1‑100 (MQ=255)
                                                                                   gCCGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGAt               >  1:2866404/1‑100 (MQ=255)
                                                                                   gCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGAt               >  1:3663243/1‑100 (MQ=255)
                                                                                     cGCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTa             <  2:2749787/100‑1 (MQ=255)
                                                                                     cGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTa             >  1:1154476/1‑100 (MQ=255)
                                                                                     cGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTa             >  2:1315016/1‑100 (MQ=255)
                                                                                     cGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTa             <  1:2018658/100‑1 (MQ=255)
                                                                                      ncTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGAatat            >  1:1533686/2‑100 (MQ=255)
                                                                                      gCTTGCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTAt            >  1:663315/1‑100 (MQ=255)
                                                                                         tGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGa         >  1:718563/1‑100 (MQ=255)
                                                                                          gCAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAg        <  2:675248/100‑1 (MQ=255)
                                                                                          gCAGGCTCAGAGGAACGGGCTGTTCAGCGAGGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAg        <  1:3553505/100‑1 (MQ=255)
                                                                                           cAGGCTCAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACACCGCACATCATCAGCCAGATTATGGAGt       >  1:3980715/1‑100 (MQ=255)
                                                                                             ggCTCAGAGTAACGGGCTGTTCAGCGAAGCCGAACTCAACCGTGAGCTGAAAAAACCACGACGTATTACCCCGCACATCATCAGCCAGATAACGGAGt       >  1:403333/1‑98 (MQ=255)
                                                                                             ggCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGttt     >  1:2248319/1‑100 (MQ=255)
                                                                                               cacaGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTtgc   >  1:1821843/1‑100 (MQ=255)
                                                                                                acaGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTtgct  <  1:668811/100‑1 (MQ=255)
                                                                                                acaGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTtgct  <  2:2412318/100‑1 (MQ=255)
                                                                                                acaGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAAACGTAAAATAAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTtgct  <  1:1644342/100‑1 (MQ=255)
                                                                                                |                                                                                                   
GCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGCT  >  NC_000913/2281183‑2281378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: